• Korean journal of applied entomology
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• v.56 no.3
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• pp.301-308
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• 2017

Locusts, Locusta migratoria (Orthoptera: Acrididae) are periodical unpredictable agricultural pests worldwide and cause serious damage to crop production; however, little consideration has been given to the management of this pest. Herein, we constructed a locust-pathogenic fungal library and confirmed that some fungi could be used as resources for locust management. First, the entomopathogenic fungi were collected from sampled soils using a Tenebrio molitor-based baiting system. For the locust assay, a locust colony was obtained from the National Institute of Agricultural Science and Technology. A total of 34 entomopathogenic fungal granules, which were produced by solid cultures, were placed in the plastic insect-rearing boxes (2 g/box) and nymphs of locust were contained in the box. In 3-7 days, mycosis was observed on the membranous cuticles of the head, abdomen, and legs of locusts. In particular, Metarhizium anisopliae, M. lepidiotae, and Clonostachys rogersoniana exhibited high virulence against the locust. Given that the 34 isolates could be used in field applications, their conidial production and stability (thermotolerance) were further characterized. In the thermotolerance assay, Paecilomyces and Purpureocillium isolates had higher thermotolerance than the other isolates. Most of the fungal isolates produced ca. >$1{\times}10^8conidia/g$ on millet grain medium. In a greenhouse trial, the granular application of M. anisopliae isolate on the soil surface resulted in 85.7% control efficacy. This work suggests that entomopathogenic fungi in a granular form can be effectively used to control the migratory locust.

• Journal of Life Science
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• v.29 no.10
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• pp.1104-1110
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• 2019

Recently, there has been an increase in the elderly population of the world. Consequently, bone metabolic diseases such as osteoporosis are emerging as a social problem. Osteoclasts play a role in bone resorption, and osteoporosis is induced when bone resorption occurs excessively. Because currently used bone resorption inhibitors may cause side effects when used for a long period of time, it is necessary to develop a new material that effectively inhibits osteoclast differentiation. This study aimed to confirm the inhibitory effect of ethanol extract of Locusta migratoria on RANKL-induced osteoclast differentiation and its mechanism. The toxicity and proliferation effects of LME on RAW264.7 osteoclasts were measured by an MTS assay. There was no cytotoxicity or proliferation when the osteoclasts were treated with up to $2,000{\mu}g/ml$ of LME. In order to confirm the effect of LME on the differentiation of osteoclasts, osteoclasts were treated with RANKL alone or with LME for 3 days. As a result of a TRAP (tartrate-resistant acid phosphatase) assay, the increasing osteoclast differentiation by RANKL decreased in a concentration-dependent manner with the treatment of LME. In addition, LME suppressed the expression of differentiation-related marker genes (TRAP, RANK, NFATc1, and CK) and proteins (NFATc1 and c-Src) that had been increased by RANKL. Also, LME influenced the $NF-{\kappa}B$, ERK and JNK signaling pathways, resulting in the inhibition of osteoclast differentiation. These results suggest that LME may be used as a novel functional material for the prevention and treatment of osteoporosis by playing a role in inhibiting bone absorption.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.371-376
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• 2017

In a nationwide survey of the occurrence and density of the migratory locust (Locusta migratoria), high density was continuously observed in the reclaimed areas of Mangun-myeon in Muan-gun, Jeollanam-do, and Sanye-myeon in Haenam-gun, Jeollanam-do, Korea. We have analyzed the nucleotide sequences of NADH dehydrogenase subunit (NAD) 2, NAD4, and NAD5 genes in order to determine the origins of the migratory locusts at two sites. According to the analysis, the migratory locusts in Haenam-gun were closely related with those in Liaoning Province and Heilongjiang Province in the northeast China. In contrast, the migratory locusts in Muan-gun were most similar to those in Japan. Because Korean migratory locusts were not included in the previous global study on the evolution and migration of migratory locusts, we did not know the origin of Korean migratory locusts, earlier. Phylogenetic analyses this study suggested that the migratory locusts from the northeast Chinese population might have migrated and settled in Haenam-gun in Korea. Moreover, another northeast Chinese population might have migrated to Muan-gun in Korea though Sakhalin, Russia and Hokkaido, Japan. However, the possibility that the migratory locusts moved from northeast China might be isolated from each other in Korea, and that the Muan population might migrate to Japan cannot be excluded.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.87-92
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• 2003

We describe here the cloning, expression and characterization of a cDNA encoding a putative chemosensory protein (CSP) from the mole cricket, Gryllotalpa orientalis. The G. orientalis chemosensory protein cDNA sequences comprised of 384 bp with 128 amino acid residues. The G. orientalis chemosensory protein showed 75.4% protein sequence identity to the Locusta migratoria CSP, Northern blot analysis revealed that signal was stronger in head than leg and cuticle, indicating that the head part containing antennae is a main site for G. orientalis chemosensory protein synthesis. The cDNA encoding G. orientalis chemosensory protein was expressed as approximately 12 kDa polypeptide in baculovirus-infected insect cells.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.1-6
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• 2001

This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.25-33
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• 2005

Recently a cDNA clone of apoLp-III from Hyphantria cunea was isolated and subjected to computational analysis to compare with other available sequences. Multiple sequence alignments were carried out using the amino acid sequences of apoLp-III from six insects. It was found that the H. cunea apoLp-III has relatively high sequence identities to Spodoptera litura ($69.5\%$), Manduca sexta ($66.8\%$), Galleria mellonella ($65.1\%$), Bombyx mori N4 ($54.3\%$) but less identity to Locusta migratoria ($18.3\%$). The amino acid composition was compared with other insects using EXPASY tools; it shows that alanine (Ala), glutamine (Gln), leucine (Leu) and lysine (Lys) are the major amino acid components of apoLp-III in H. cunea as well as other lepidopterans. Homology modeling performed using PSI-BLAST (PDB template M. sexta) reveals that the apoLp-III molecules consist of five, long amphipathic alpha helical bundles with short loops connecting the helices and shows homology with other insects. Phylogenetic analysis shows that the orthopteran apoLp-III represented by locust was most distantly related to the lepidopteran insects.

• Animal cells and systems
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• v.1 no.3
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• pp.475-482
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• 1997

The antiserum against locustatachykinin I, originally isolated from brain and retrocerebral complex of the locust Locusta migratoria, has been used to investigate changes in number, localization, and structure of locustatachykinin I-immunoreactive (LomTK I-IR) neurons in the brains of the common cutworm, Spodoptera Iitura, during postembryonic development. These neurons are found at larval, pupal, and adult stages. In the larval stages, the first instar larva shows the first appearance of about 8 LomTK I-IR neurons. These neurons gradually increase in number from the second to fourth instar larvae which have the largest number of about 92 in all postembryonic stages. Thereafter, these neurons decrease to about 28 in number in the 5-day-old pupa. However, they begin to rise again from the 7-day-old pupal stage, eventually reaching to about 90 in the l-day-old adult. The developing larval brains contain cell bodies of these neurons in most neuromeres. After the metamorphosis of larva to pupa and adult, localization of these neuronal cell bodies is confined to the specific cerebral neuromeres. The 7-day-old pupal brain shows the location of these neuronal cell bodies in pars intercerebralis, pars lateralis of protocerebrum, deutocerebrum, tritocerebrum, optic lobe-near region, and subesophageal ganglion. In the l-day-old adult, however, the brain has these cell bodies only in some neuromeres of protocerebrum, deutocerebrum, and subesophageal ganglion. Throughout the postembryonic life, changes in structure of these neurons coincide with changes in number and localization of these neurons. These findings suggest that changes in number, localization, and structure of these neurons reflect differentiation of these neurons to adult type.

• Journal of Sericultural and Entomological Science
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• v.53 no.1
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• pp.29-37
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• 2015

Since based on the revised educational curriculum, the insects and spiders in eight biology textbooks I and II of high school are as follows. In class level, two classes, Insecta and Arachnida were investigated and their frequence numbers in the textbooks were a total of 143 times. In order level, 12 orders in insecta, 2 orders in Aranea were appeared in the textbooks. The most appearing frequence number was represented in Lepidoptera (33 times) and then in Araneae (11 times). For each publisher, the most appearing frequencies of insecta and spiders was shown in Gyohaksa (23 times), and then in Hyeongseol and Centurla education research institute (21times, respectively). In the textbooks, four species were found to misidentify in their species name and five mistakes were investigated in their photos and content.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.11-17
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• 2005

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.1
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• pp.1-15
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• 2001

The purpose of this study is to evaluate the creation techniques of eco-pond, one of biotopes to promote biodiversity in urban residence area. Investigation were classified out plant, mammals, amphibia, reptiles, birds, fishes and insects. The results were summarized as follows: Around the eco-pond shows simple vegetation structurs, consisted of Pinus densoflora S et Z. and Robinia pseudoacacia under competition. In case of shrub, consisted of 4 species but plant growing appearence diversely by seasons. The evaluation of vegetation of eco-pond, there are found 4 species of aquatic plants. Inside the revetment of pond, Echinochloa crus-galli, Persicaria hydropiper, Digiaria sanguinalis, Cyperus microiria and Bidens frondosa L. are mainly distributed. Near the revetment, Trifolium repens L. and Digiaria sanguinalis are prevailed. And in its background, Erigeron canadensis, Erigeron annuus and vines are begins to make their appearances. When evaluation animals in eco-pond and contrast plot, it show simple species and numbers of mammals. It seemed to be resulted from its isolation and outside intervention by users In eco-pond, Pica pica and Streptopelia orientalis are mainly found and in contrast plot of Columba livia, which are so strong adaptation to city life environment. In case of amphibia and reptiles, none is observed in contrast plot, but in ecological pond, Rana nigromaculata and Hyla japonica are constantly observed. In case of insects, more species are found in eco-pond than contrast plot. And in eco-pond, more dragonflies are visibly increased one year after its construction. In floral zone inside of pond revetment, grasshopper and Locusta migratoria are frequently observed. In case of butterflies, they are mainly found in log fence and willow(salix) around eco-pond. In case of fishes inside of eco-pond, the species and its density are remarkable increased one year after the construction. With above evaluation results, we have identify the increase effect of biodiversity after construction of the eco-pond.