• 한국동물학회지
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• 제36권2호
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• pp.209-222
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• 1993

The present study attempts to explore the first appearance 8nd subsequent development of %cosine hvdroxvlase (TH)-containing neurons in the rat brain from embryonic day (E) 10.5 to the neonate. To increase the senti노ivity and resolution power, a double bridge peroxidase-anti-peroxidase technique was employed for cellular localization of TH. In situ hybridization histochemistw with synthetic TH oligomer (30-mer) codinB TH was also used to detect TH mRNA. TH-containing neurons were first detected at E11.5 in the intermediate zone of prosencepha1on and mesencephalon. At this stage, TH-immunoreactive neurons were small, ovoid bee and emitted their fibres into their immediate surroundings. From this stage, TH-immunoreactive neurons increased in their number and underwent migration and cell differentiation. At E15.5, the distribution pattern of the maior groups of TH neurons was similar to that of adult catecholaminergic groups, and at E19.5 the external laver of median eminence showed TH-immunoreactive processes.

• Molecules and Cells
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• 제20권2호
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• pp.256-262
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• 2005

The neuronal cytoskeleton is essential for establishment of neuronal polarity, but mechanisms controlling generation of polarity in the cytoskeleton are poorly understood. The nonreceptor tyrosine kinase, Fer, has been shown to bind to microtubules and to interact with several actin-regulatory proteins. Furthermore, Fer binds p120 catenin and has been shown to regulate cadherin function by modulating cadherin-${\beta}$-catenin interaction. Here we show involvement of Fer in neuronal polarization and neurite development. Fer is concentrated in growth cones together with cadherin, ${\beta}$-catenin, and cortactin in stage 2 hippocampal neurons. Inhibition of Fer-p120 catenin interaction with a cell-permeable inhibitory peptide (FerP) increases neurite branching. In addition, the peptide significantly delays conversion of one of several dendrites into an axon in early stage hippocampal neurons. FerP-treated growth cones also exhibit modified localization of the microtubule and actin cytoskeleton. Together, this indicates that the Fer-p120 interaction is required for normal neuronal polarization and neurite development.

• 한국동물생명공학회지
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• 제38권3호
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• pp.89-98
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• 2023

Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.

• 대한전자공학회논문지SD
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• 제45권6호
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• pp.49-59
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• 2008

본 논문에서는 SIFT(Scale Invariant Feature Transform) 알고리즘을 임베디드 환경에서 실시간으로 처리하기 위해 가장 연산량이 많은 특징점 위치 결정 단계를 고정 소수점 모델로 설계 및 분석하고 그에 근거한 하드웨어 구조를 제안한다. SIFT 알고리즘은 객체의 꼭지점이나 모서리와 같이 색상 성분의 차가 심한 구역에서 얻어진 특징점 주위 픽셀의 벡터성분을 추출하는 알고리즘으로, 현재 얼굴인식, 3차원 객체 인식, 파노라마, 3차원 영상 복원 작업의 핵심 알고리즘으로 연구 되고 있다. 본 알고리즘에 대한 최적의 하드웨어 구현을 위해 특징점 위치(Keypoint Localization)와 방향(Orient Assignment)에 대한 정확도, 오차율을 사용하여 고정 소수점 모델에서 각 중요 변수들의 비트 크기를 결정 한다. 얻어진 고정 소수점 모델은 원래의 부동 소수점 모델과 비교했을 때 정확도 93.57%, 오차율 2.72%의 결과를 보이며, 고정 소수점 모델은 부동 소수점 모델과 비교하여 제거된 특징점의 대부분이 두 영상에서 추출된 특징점 끼리의 매칭과정에서 불필요한 객체의 모서리 영역에 몰려있음을 확인했다. 고정 소수점 모델링 결과 ARM 400MHz 환경에서 약 3시간, Pentium Core2Duo 2.13GHz 환경에서 약 15초의 연산시간을 갖는 부동 소수점 모델이 동일한 환경에서 약 1시간과 10초의 연산시간을 가지며, 최적화된 고정 소수점 모델을 하드웨어로 구현 시 $10{\sim}15\;frame/sec$의 성능을 보일 것으로 예상한다.

• Parasites, Hosts and Diseases
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• 제39권4호
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• pp.307-312
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• 2001

In the course of Clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. The antigenic proteins stimulating IgG antibody production were chronologically identified by immunoblot and localized by immunogistochemical staining. In the early stage of infection until 12 weeks post-infection (PI) , antigens were proteins with molecular mass larger than 34 kDa which were derived from the tegument, testes and intrauterine eggs. After 20 weeks PI antigens recognized were 29, 27 and 26 kDa proteins from the intestine, excretory bladder and reproductive organs. It is suggested that the tegumental proteins are the most potent antigens and the excretory-secretory proteins with middle molecular mass of 26-45 kDa contribute to the high level production of antibodies after 20 weeks of the C. sinensis infection.

• 한국실내디자인학회논문집
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• 제15권3호
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• pp.33-39
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• 2006

This paper describes Modernization of the public dwelling focused on DK type standard plan during 1950's. in Japan. Shortage of the dwelling stated after World War II, inferior housing environment, required the re-reflection of a dwelling and a life system succeeded to traditionally As a result, it was going to solve a principle application of a new life style for a demand of a development process of the modernization through DK type standard plan. Although it was thought that the modernization process of the public dwelling Japan had aimed at Westernization, it turned out that modernization was developed according to the peculiar housing culture system of Japan. As for it, in the planning stage of DK plan, although known with the public and private space for which the Western-like element was generally accommodated by modernization processes, such as closing-izing of space, and specialization of a function, generally, succession of a traditional element and a modernistic change of that are seen. Moreover, it can be said that Westernization was transcended and peculiar localization of Japan was fixed.

• 비파괴검사학회지
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• 제34권6호
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• pp.419-427
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• 2014

Nonlinear features of ultrasonic waves are more sensitive to the presence of a fatigue crack than their linear counterparts are. For this reason, the use of nonlinear ultrasonic techniques to detect a fatigue crack at its early stage has been widely investigated. Of the different proposed techniques, laser nonlinear wave modulation spectroscopy (LNWMS) is unique because a pulse laser is used to exert a single broadband input and a noncontact measurement can be performed. Broadband excitation causes a nonlinear source to exhibit modulation at multiple spectral peaks owing to interactions among various input frequency components. A feature called maximum sideband peak count difference (MSPCD), which is extracted from the spectral plot, measures the degree of crack-induced material nonlinearity. First, the ratios of spectral peaks whose amplitudes are above a moving threshold to the total number of peaks are computed for spectral signals obtained from the pristine and the current state of a target structure. Then, the difference of these ratios are computed as a function of the moving threshold. Finally, the MSPCD is defined as the maximum difference between these ratios. The basic premise is that the MSPCD will increase as the nonlinearity of the material increases. This technique has been used successfully for localizing fatigue cracks in metallic plates.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2011년도 제36회 춘계학술대회논문집
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• pp.179-182
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• 2011

비행축/자세제어용 단일추진제 추력기의 우주발사체 적용과 국내 설계개발 현황을 조사/분석하였다. 탑재체의 정확한 궤도투입을 위하여 정밀한 자세제어가 요구되는 발사체 최종 단은 대부분 비추력 성능이 우수하고 높은 신뢰도 확보가 가능한 단일추진제 하이드라진 추력기시스템을 장착한다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 제5회 압연심포지엄 신 시장 개척을 위한 압연기술
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• pp.307-316
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• 2004

This study examined the cause of the wrinkle defect which is frequently encountered in wire rod rolling of low carbon steel$(C0.08\~0.13wt.\%)$. Even a small defect on the surface of rolled bars can easily develop into fatal cracks during cold heading process of low carbon steel, and it is therefore necessary to minimize inherent defects on the surface of hot rolled bars. Hot rolling process of low carbon steel was analyzed to identify the cause of the wrinkle defect in conjunction with FE analysis. The integrated analysis revealed that the wrinkle defect initiated in the first stage of rolling, and it was at the billet edge where severe deformation and drastic temperature drop were present. To elucidate the micro-mechanical mechanism of the wrinkle defect, hot compression tests were carried out at various temperatures and strain rates using Gleeble-3800. The surface profile of the each other compressed specimens was compared, and rough surface lines were observed at relatively low temperatures. Those surface defects can develop into wrinkles during multi-pass rolling. To control the wrinkle defect in rolling, it is necessary to design an adequate caliber which can minimize the loss of ductility, and thereby prevent flow localization. To use the result of this study fur other steels, the quantitative measure of the wrinkle defect and flow localization parameter should be proposed.

• 한국동물학회지
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• 제35권2호
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• pp.149-160
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• 1992

Using the protein A-gold complex, the mvoabrillogenesis and actin localization of cultured myoblast were invastisated. In the superstructural changes of mvogenic cell during differentiation, pectoral myoblasts contained large nucleus and numerous ribosomes but no myofibrils during the first 24 hr of cultures. Mvoblast initiated to differentiate at 3-day of culture contained the primitive myofibrillar structure. At 96 hr of culture, the mvofibrillar structure showed reletively discernable Z band but pools defined A, H and M bands. The feature of sarcomeric structure showed more defined form at cultur 5 day. In the aspect of actin localization, actin wvas diffusely detected throughout the cytoplasm of myogenic cell and nucleus during the proliferating stage. At 72 hr of culture, with the appearantc oi primitive mvofibrils, gold particles were observed in surrounding of myofibrils but still presented in overall of cytoplasm, especially in the surface and lumen of endoplasmic reticulum. With the gradual increase of culture time, local distribution of actin was readily detected within cytoplasm. In the 5-day specimen of cultures, gold particles precisely indicate the sites of actin localifation within the sarcomere. These results indicate the time of onset of myofibrill appearance and the biosynthetic and incorporation pathway of actin molecules into sarcomeric structure during myofibrillogenesis. Thus, in the present study, the first mvoabrillar structure was detected at culture 3 day, and the initiation of assembly into a typical sarcmeric structure was observed at culture 5 day. It seems, however, that the course of events on myofibrillogenesis of cultured myoblasts can be changed with great dependence of culture conditions including the number and groluth rate of mononucleated mvoblasts after seeding although the fundamental process shows identical appearances.