• Parasites, Hosts and Diseases
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• 제52권6호
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• pp.595-603
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• 2014

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time-and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.

• Parasites, Hosts and Diseases
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• 제59권6호
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• pp.573-583
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• 2021

Toxoplasma gondii, an intracellular protozoan parasite that infects one-third of the world's population, has been reported to hijack host cell apoptotic machinery and promote either an anti- or proapoptotic program depending on the parasite virulence and load and the host cell type. However, little is known about the regulation of human FHs 74 small intestinal epithelial cell viability in response to T. gondii infection. Here we show that T. gondii RH strain tachyzoite infection or ESP treatment of FHs 74 Int cells induced apoptosis, mitochondrial dysfunction and ER stress in host cells. Pretreatment with 4-PBA inhibited the expression or activation of key molecules involved in ER stress. In addition, both T. gondii and ESP challenge-induced mitochondrial dysfunction and cell death were dramatically suppressed in 4-PBA pretreated cells. Our study indicates that T. gondii infection induced ER stress in FHs 74 Int cells, which induced mitochondrial dysfunction followed by apoptosis. This may constitute a potential molecular mechanism responsible for the foodborne parasitic disease caused by T. gondii.

• 한국항해항만학회지
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• 제34권7호
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• pp.525-532
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• 2010

Mobile Harbor (MH) is a new transportation platform that can load and unload containers onto and from very large container ships at sea. It could navigate near harbors where several vessels run, or it could navigate through very narrow channels. In the conceptual design phase when the candidate design changes frequently according to the various performance requirements, it is very expensive and time-consuming to carry out model tests using a large model in a large towing tank and a free-running model test in a large maneuvering basin. In this paper, a new Planar Motion Mechanism(PMM) test in a Circulating Water Channel (CWC) was conducted in order to determine the hydrodynamic coefficients of the MH. To do this, PMM devices including three-component load cells and inertia tare device were designed and manufactured, and various tests of the MH such as static drift test, pure sway test, pure yaw test, and drift-and-yaw combined test were carried out. Using those coefficients, course-keeping stability was analyzed. In addition, the PMM tests results carried out for the same KCS (KRISO container ship) were compared with our results in order to confirm the test validity.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2000년도 전력전자학술대회 논문집
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• pp.493-497
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• 2000

A battery is the device that transforms the chemical energy into the direct-current electrical energy without a mechanical process. Unit cells are connected in series to obtain the required voltage while being connected in parallel to organize capacity for load current. Because the voltage drop down in one set of battery is faster than in two one it may result in the low efficiency of power converter with the voltage drop and cause the system shutdown. However when the system being shutdown. However when the system being driven in parallel a circular-current can be generated,. It is shown that as a result the new batteries are heated by over-charge and over-discharge and the over charge current increases rust of the positive grid and consequently shortens the lifetime of the new batteries. The difference between the new batteries and old ones is the amount of internal resistance. In this paper we can detect the unbalance current using the microprocessor and achieve the balance current by adjusting resistance of each set, The internal resistance of each set becomes constant and the current of charge and discharge comes to be balanced by inserting the external resistance into the system and calculating the change of internal resistance.

• 전기학회논문지
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• 제58권8호
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• pp.1638-1643
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• 2009

Uroflowmetry is a convenient clinical test to screen the benign prostatic hyperplasia(BPH) common in the aged men. A load cell is located beneath the urine container to measure the weight of urine. However, it is sensitive to the impact applied on the bottom of the container by the urine stream, which could be a noise source lowering the reliability of the system. With this aim, our study proposed a noise reduction technique by computing ensemble average of the weighted signals that were acquired from three-load cells forming a regular triangle beneath the urine container. Simulated urination experiment was performed with three different collection methods, all of which demonstrated significant noise reduction by ensemble averaging. Furthermore, the best results can be obtained without any special urine collection devices. Thus, our novel method can be usefully applied to uroflowmetry for enhancing measurement in terms of accuracy and reliability.

• Structural Engineering and Mechanics
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• 제76권2호
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• pp.175-191
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• 2020

In this study, the effect of Nano silica (SiO2) on the buckling strength of the glass fiber reinforced laminates containing the machining process causes holes were investigated. The tests have been applied on two status milled and non-milled. To promote the mechanical behavior of the fiber-reinforced glass epoxy-based composites, Nano sio2 was added to the matrix to improve and gradation. Nano sio2 is chosen because of flexibility and high mechanical features; the effect of Nanoparticles on surface serenity has been studied. Thus the effect of Nanoparticles on crack growth and machining process and delamination caused by machining has been studied. We can also imply that many machining factors are essential: feed rate, thrust force, and spindle speed. Also, feed rate and spindle speed were studied in constant values, that the thrust forces were studied as the main factor caused residual stress. Moreover, entrance forces were measured by local calibrated load cells on machining devices. The results showed that the buckling load of milled laminates had been increased by about 50% with adding 2 wt% of silica in comparison with the neat damaged laminates while adding more contents caused adverse effects. Also, with a comparison of two milling tools, the cylindrical radius-end tool had less destructive effects on specimens.

• 상하수도학회지
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• 제20권3호
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• pp.329-338
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• 2006

The research aims to provide the basic data for practical applications by correlating the bio-kinetic coefficients with the load of recalcitrant organic matter in box-mill wastewater. The activated sludge process was employed to a Wastewater disposal plant in an industrial setting, increase of consequently leading to the organic load. The parameter values derived by Monod-kinetic analysis were as follows:specific substrate removal rate $K_{max}=0.17day^{-1}$, half saturation constants $K_s=60.37mg/l$, decay coefficient $K_d=0.142day^{-1}$, microbial yield coefficient y = 0.388mg/mg, and max specific growth rate ${\mu}_{max}=0.006day^{-1}$. In view of biodegradability, the $TCOD_{Mn}/TBOD_5$ ratios of inflow and outflow were 1.07 and 1.41, and the $SCOD_{Mn}/SBOD_5$ ratios of inflow and outflow were 1.10 and 1.50, respectively. The higher $TCOD_{Mn}/TBOD_5$ ratio of outflow indicated that metabolites of a microorganism have accumulated in the cells.

• 센서학회지
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• 제27권4호
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• pp.242-248
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• 2018

Bicycle fittings have been used to ride bicycles comfortably while minimizing non-traumatic injuries. To analyze the cause of non-traumatic injuries, it is necessary to measure the body weight distribution in various biking positions. In this study, a weight distribution measurement system was implemented by installing five weighable devices on the saddle, both pedals, and both handle grips of a bicycle. To measure the body weight applied through the saddle, the structure of a commercial seat post was modified and a load cell was installed inside. Weighable pedals and handle grips were designed using a 3D modeling program and fabricated by employing a 3D printer. The body weight distribution for ten bicycle riders was measured when the two pedals were aligned horizontally and vertically. Experimental results showed that the body weight distribution varied significantly depending on human body shape, even after the bicycle fitting was completed. The difference between the body weight measured by the proposed system and a commercial scale was less than 3 %.

• 한국기계가공학회지
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• 제8권4호
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• pp.97-103
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• 2009

Since a demand for an extensive range of earthmoving equipment like a hydraulic excavator with a capacity of 85tons has been asked in construction fields, a performance of its track drive unit has to be verified experimentally. Among the verification of the performances, a torque measurement is at issue, in which a torque meter is utilized widely. However, the very large scaled torque meter is necessary when a discharged torque from the track drive unit is increased significantly. In addition, the price for experimental set-up of a torque meter is too high due to its limitation of the geometry such as long length, and a break down in operation occurs frequently. In this study, to measure a high torque up to 200,000Nm, a new technique was proposed as an alternative of conventional measurement by using a torque meter. The new technique enables to measure the high torque stably in a compact space via a torque arm and two load cells. The experimental results showed a propriety of the proposed torque measuring technique for a track drive unit with very large capacity.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 추계학술대회 초록집
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• pp.92.1-92.1
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• 2011

Anode off-gas of high temperature fuel cells such as MCFC contains a significant amount of combustible components like hydrogen, carbon monoxide and methane according to fuel utilization ratio of the fuel cell stack. Thus, it is important to fully burn anode off-gas and utilize the generated heat in order to increase system efficiency and reduce emissions as well. In the present study, 25 kW catalytic combustor has been developed for the application to a load-following 300kW MCFC system. Mixing and combustion characteristics have been experimentally investigated with the catalytic combustor. Since the performance of catalytic combustor directly depends on the combustion catalyst, this study has been focused on the experimental investigation on the combustion characteristics of multiple catalysts having different structures and compositions. Results show that the exhaust emissions are highly dependent on the catalyst loading and the ratio of catalytic components. Test results at load-following conditions are also shown in the present study.