• Journal of Animal Environmental Science
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• v.16 no.2
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• pp.143-152
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• 2010

Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

• The Journal of the Petrological Society of Korea
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• v.10 no.3
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• pp.157-171
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• 2001

This study investigated the effects of Pb/Tl ratio, Pb concentration and concomitant matrix elements on the measurement of Pb isotope ratios using multi-collector ICP/MS (AXIOM MC model). Accuracy and reproducibility of Pb isotope ratios in NBS 981 solution were estimated for 42 data measured from March to August 2001. Pb isotopes measured in rocks, bronzes and sediments were compared to data measured by TIMS. Reproducibilities for $^{206}Pb/^{204}Pb,\; ^{207}Pb/^{204}Pb,\;and\;^{208}Pb/^{204}Pb$ ratio were about 500 ppm (2sd) and for $^{207}Pb/^{206}Pb$\;and\;^{208}Pb/^{206}Pb$ were 100~200 ppm for 200 ng of Pb in NBS 981 solution. The optimum conditions for the analysis of Pb isotope ratios with AXIOM MC for best accuracy and reproducibility were defined as follows; 1) Pb/Tl ratio is about 10 2) Pb concentration is about 100 ng/ml 3) correction for mass discrimination is performed by exponential law using 2.3887 of $^{205}Tl/^{203}Tl$ and Pb mass fractionation factor empirically obtained from $ln(^{208}Pb/^{206}Pb)-ln(^{205}Tl/^{203}Tl)$ relationship. The sample data measured with MC/ICP/MS for acid-digested and chemically separated rock samples, and acid-digested bronze samples and sediment samples coincide with those of TIMS within analytical errors. Therefore, MC/ICP/MS is a rapid analytical technique for Pb isotope ratios with the similar precision compared with TIMS.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.233-240
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• 1999

Understanding of the relationships between seeding rate and planting date is important for production of spring oats during the fall season. Therefore, this experiment was conducted to investigate seeding rate and planting date effects on forage performance and quality of spring oats(Avena sativa L.) at Suweon in 1997. The experiment was arranged in a split plot design with three replications. The main plots consisted of different seeding rates such as 150, 200, and 250kg/ha. The subplots consisted of different planting dates of spring oats such as 15, 22, and 29 August and 5 September. Dry matter content of oats was significantly decreased(P<0.01) as the planting date was delayed except the planting date of 5 September(24.8%). Dry matter content of forage oats planted on 15, 22, and 29 August was 23.0, 21.5, and 20.5%, respectively. A seeding $rate\;{\times}\;planting$ date interaction for dry matter content was highly significant(P<0.01). Crude protein content of oats was significantly increased(P<0.01) from 15.6 to 27.9% as the planting was delayed, while a significant seeding $rate\;{\times}\;planting$ date interaction for crude protein content of oats was observed. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents of oats increased with early planting(P<0.01), but there was no significant difference among the seeding rates. ln vitro dry matter digestibility(IVDMD) of oats was significantly increased(P<0.01) as the seeding rate was increased together with late planting. Average dry matter yield of oats decreased(P<0.01) from 2,647 to 615kg/ha as the planting was delayed, but there was no significant difference among the seeding rates. A seeding $rate\;{\times}\;planting$ date interaction was observed for average dry matter yield of oats that the seeding rate increase in this experiment showed better at early planting dates. Crude protein yield of oats increased from 171 to 410kg/ha as the planting was delayed, and a significant increases(P<0.01) for crude protein yield was found among the seeding rates. ln vitro digestible dry matter(IVDDM) yield of oats decreased(P<0.01) as the planting was delayed, but there was no significant difference among the seeding rates. A significant seeding $rate\;{\times}\;planting$ date interaction for lVDDM of oats was found. The results of this study indicate that both the seeding rate of 200kg/ha and early planting before 29 August would be recommendable for forage yield and quality of oats in Korea.