• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.2
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• pp.176-184
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• 2014

Nowadays, consumption of fisheries products is increasing. There are several factors, one of which is a quantitative development through aquaculture. Another factor is an increase qualitative consumption of fish which require that fish be supplied alive. This requires a lot of technical effort to transport the live fish that have low survival rate (c.f. tuna and mackerel) in coastal waters and in the open sea. To develop a towing cage for transporting the live fish, model test in a circulate water channel and simulation by computer tool were carried out. In order to spread vertically, floats were attached at the upper part of the cage, and iron chains attached at the lower part of the cage. For horizontal spreading, kites were attached on the cage. The tension and spreading performance of the cage were measured. The result shows that the tension and reduction ratio of inside volume of the cage were tended to increase with increased towing speeds. The suitable operation condition in towing cage was 1.0 m/s towing speeds with vertical spreading force 8.7 kN, horizontal spreading force 5.6 kN; in this case the reduction ratio of inside volume of the cage was estimated as 25%.

• Fisheries and Aquatic Sciences
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• v.26 no.10
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• pp.583-592
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• 2023

Celebes rainbow (Marosantherina ladigesi) is one of Indonesia's exported ornamental fish commodities, but the exploitation of this fish only relies on wild catches. The rise of unlimited fishing, especially those using poison, has changed the aquatic environment, threatening sustainability and causing fish extinction. This study aimed to evaluate the effectiveness of several types of feed in improving the absolute growth rate (AGR), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), feed efficiency (FE), hematology, and immune response of Celebes rainbow. The fish used in this study were male ornamental Celebes rainbow (M. ladigesi) weighing 1.32 ± 0.21 g/ind, reared in 54 L-aquariums at a stocking density of 30 individuals/aquarium for six weeks. The fish were fed according to the test diet consisting of live Tubifex sp worms, dry Tubifex sp worms, Spirulina platensis, and crumble pellets. The parameters observed were AGR, SGR, SR, FCR, FE, hematology, intestinal histology, liver histology, and a challenge test with the pathogenic bacteria Aeromonas hydrophila. The results showed that fish-fed live Tubifex sp worms had better AGR, SGR, SR, FCR, FE, hematology, and disease resistance compared to all other treatments. These results indicate that live Tubifex sp worms are the best feed for rearing Celebes rainbow.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.542-544
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• 2002

This study was carried out to evaluate the ciliates Euplotes sp. as a live food for marine fish larvae. The ciliates and the rotifers Brachionus plicatilis, which were cultured with the baker's yeast Saccharomyces cerevisiae and the $\omega$-yeast emulsified with cuttle fish liver oil, were supplied to the larvae of flounder Paralichthys olivaceus and grouper Epinephelus akaara. Considering the size difference between the ciliates 68 $\cdot$ 7 $\mu$n and the rotifers 160 $\cdot$ 20 $\mu$n, the rotifers and ciliates were supplied to the larvae tank with the density of 2 inds./mL and 20 inds./mL, respectively. The survival rate and growth in length of the flounder larvae fed on rotifer were significantly higher than those on Euplotes sp.. In grouper larvae which have a small mouth diameter, even the survival rate of the larvae fed on the ciliates was better than that on the rotifers, it was very low less than $20\%$. Therefore, Euplotes sp. seem to be incongruent as a live food for marine fish larvae.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.2
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• pp.78-84
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• 2006

A high performance liquid chromatography assay method for spiramycin in fish muscle was developed. The developed method was evaluated and validated by monitoring spiramycin in olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli) and in live conger eel (Anguilla japonica) in fish farms and distribution centers. Using the developed method, the recovery rate was up to 82.4-88.8%, which was higher than that of conventional methods (77.6-87.1%). In particular, the proposed sample treatment protocol was suitable for use with fish samples to remove low molecular weight materials and pigments that could interfere an accurate analysis. The prepared stock solution was very stable, and it remained chemically stable for 5 weeks at $4^{\circ}C$. The performance limit of the developed method for spiramycin acid in fish muscle was 0.05 ppm. One hundred thirty-four fish samples including olive flounder, black rock fish and live conger eel were analyzed to evaluate the overall efficiency of the modified method and to monitor the actual condition of spiramycin usage in fish farms. Olive flounder and black rock fish were collected from fish farms in coastal areas of Korea, and live conger eels were purchased from a fish market in the Busan area from September 2001 to March 2002. According to the overall performance of the developed method, it was considered suitable for the monitoring of spiramycin in fish muscle. The suggested method of analysis for spiramycin in fish muscle is registered in the Korean Official Methods of Food Analysis and is currently tieing utilized for fishery products inspection by the Korea Food and Drug Administration and the National Fisheries Products Quality Inspection Service.

• Ocean and Polar Research
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• v.36 no.2
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• pp.199-208
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• 2014

Copepods are a major food source for marine fish larvae in nature. Many studies on copepods culture have been conducted to develop a new live food for the seedling production of marine fish larvae. But fish farmers still depend on rotifer and Artemia nauplii. This study was carried out to find suitable copepods as live food for the larvae in hatchery. Eight species of copepods (1 calanoid, 2 cyclpoid, 5 harpacticoid) that were fed Isochrysis galbana were examined in terms of the size of nauplii, fecundity, amino acids, and fatty acids contents. These species were divided into small (nauplii length 46-86 ${\mu}m$) and large (nauplii length 120-188 ${\mu}m$) size group. Nitokra spinipes in the small group and Tigriopus japonicus in the large group showed the highest fecundity with 151.1 and 139.6 nauplii production per gravid female, respectively. With regard to nutrients, essential amino acids were the highest with 21.2% in cyclopoid Paracyclopina nana in the small group and n-3 HUFA were the highest in calanoid Pseudodiaptomus inopinus (8.5 ${\mu}g/mg$) in the large group and P. nana (8.8 ${\mu}g/mg$). In terms of the size, fecundity, and nutritional value of copepods examined in this study, N. spinipes and P. nana seem to be suitable copepod species to develop as a new live food for small mouth fish larvae.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.155-158
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• 1996

Seventy two Black Bengal goats on road side grass based diet were fed on 0, 20, 40, 80, 100 or 120 g of fish meal (FM)/goat/day with or without ad libitum access to urea molasses block(UMB). The purpose was to assess the interaction of undegradable protein (UDP) and fermentable nitrogen(N) supplementation on feed intake, nutrient digestibility and growth of goats. Live weight gain of goats increased linearly with the increasing of dietary fish meal as an undegradable protein source. With the increasing level of fish meal the live weight gain(g/day) was 17, 23, 46, 48, 48 and 52 with urea molasses block and 12, 21, 31, 49, 47 and 47 without urea molasses block. It was concluded that the beneficial effects of urea molasses feeding to accelerate the dry matter intake, TDN intake and nutrient digestibility observed could not be exploited in terms of live weight gain.

• Journal of fish pathology
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• v.34 no.1
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• pp.99-104
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• 2021

In this study, we investigated safety and efficacy of a low temperature immunization protocol with NNV in red spotted grouper, Epinephelus akaara and long tooth grouper, Epinephelus bruneus and seven band grouper, Hyporthodus septemfasciatus. Further, growth rate between immunized and naïve fish was evaluated during the experiment to check side effect of immunization. Three grouper species were immunized by immersion method with live NNV at 10^5.0 TCID₅₀/mL at 16.5℃ for 30 min and reared for 120 days at natural sea water temperature. To evaluate growth rate, total length and wet weight was measured 7 times after immunization. Immunized three grouper species were challenged by intramuscular inoculation with NNV at 10^4.2 TCID₅₀/100 µL/fish. Immunization at low temperature with live NNV did not show any clinical symptoms of infection, mortality and inhibition of growth. After challenge, cumulative mortality of naïve seven band grouper, red spotted grouper, long tooth grouper were 45, 10, 20 %, respectively. However no mortality was observed at immunized groupers. Thus, it was demonstrated that immunization at low temperature with live NNV are able to protect three different species of groupers without inhibition of growth.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.2
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• pp.233-243
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• 2015

Controlled broadband acoustic scattering laboratory experiments were conducted using a linear chirp signal (95-220 kHz), and x-ray images of live and model fish with an artificial swim bladder were analyzed to investigate the changes in orientation and frequency dependence of target strength (TS) due to morphological differences in fish swim bladders. The broadband echoes from live and model fish were measured over an orientation angle range of ${\pm}45^{\circ}$ in the dorsal plane and in approximately $1^{\circ}$ increments. The location of nulls in the simulated echo response of the SINC [sinc function] model was overlaid on the TS map, showing the orientation and frequency dependence of fish TS, and they matched very well. It was possible to infer the equivalent fish scattering size (or swim bladder) using the null spacing in the experimentally obtained broadband TS map. Good agreement was observed for inferring the equivalent scattering size between the SINC model and the broadband echoes measured for the three fish species (black scraper Thamnaconus modestus; goldeye rockfish Sebastes thompsoni; and whitesaddled reef fish Chromis notatus). Some results of this inference are discussed.

• The Journal of Fisheries Business Administration
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• v.37 no.1 s.70
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• pp.45-71
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• 2006

The main objective of this study is to analyze problems of the Country of Origin Labeling(COOL) in small live fish(SLF) trade by applying the asymmetric information theory which is called ${\ulcorner}$Lemon Market Theory${\lrcorner}$. The purpose of importing SLF is to enhance fishing household income and import substitution effect as well. SLF importation is quite different in its nature from importing general fish and fish products for final/direct consumption. SLF are imported for the purpose of domestic aquaculture and naturalization where domestic production/supply of SLF is less than domestic demand for or such species are not native to Korean waters. Information asymmetric problems arise even in SLF trade as the same way in general goods and services. However, the information asymmetry issues in SLF trade are much more complex than non-living goods because SLF are traded in the live state. To alleviate such problems the Korean government initiated the general COOL scheme to imported SLF. However, many experts argues that such policy on SLF would not be appropriate because of SLF's very nature. Applying the lemon market theory, we can analyze how information discovery schemes are able to signal correct information to SLF trading parties and to result in more symmetric information in SLF trade markets. This research carried out a case study about small live eel(SLF) trade and its farming. The results showed that applying the COOL to small live eels just right upon coming into fish farms tends to increase substantially fish farmer's income and at the same time to lower unnecessary transaction costs. In particular, such transaction costs by imposing simply the general COOL on SLF may easily outweigh its benefits. For instance, to resolve the problems, the Korean ministry of agriculture and forestry(KMAF) has developed a quite different COOL rules from the general ones and has applied them to imported live cattle and medium/small livestocks. The KMAF's differentiated COOL policy on some imported livestocks may be a good case which can be applied to imported SLF. In addition to the differentiated COOL on SLF, Sanitary and Phytosanitary (SPS) and traceability system would play crucial complementary roles in alleviating information asymmetry problems in SLF trade. Advanced fisheries tend to strengthen their SPS system rather than to adopt the general comprehensive COOL schemes into imported SLF trades and domestic market exchanges.

• Journal of fish pathology
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• v.37 no.1
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• pp.17-23
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• 2024

Spring viremia of carp virus (SVCV) poses a significant threat to numerous cyprinid fish species, particularly the common carp (Cyprinus carpio), often resulting in substantial mortalities. This study explores the potential use of a chimeric recombinant snakehead rhabdovirus carrying the SVCV G gene (rSHRV-Gsvcv) as a live vaccine against SVCV infection. Through virulence testing in zebrafish at different temperatures (15 ℃ and 20 ℃), no mortality was observed in groups infected with either rSHRV-wild or chimeric rSHRV-Gsvcv at both temperatures, whereas 100% mortality occurred in fish infected with wild-type SVCV. Subsequently, as no mortality was observed by rSHRV-Gsvcv, three independent experiments were conducted to determine the possible usage of chimeric rSHRV-Gsvcv as a vaccine candidate against SVCV infection. Fish were immunized with either rSHRV-Gsvcv or rSHRV-wild, and their survival rates against the SVCV challenge were compared with a control group injected with buffer alone at four weeks post-immunization. The results showed that chimeric rSHRV-Gsvcv induced significantly higher fish survival rates compared to rSHRV-wild and the control groups. These findings suggest that genetically engineered chimeric rSHRV-Gsvcv holds the potential for a prophylactic measure to protect fish against SVCV infection.