• Title/Summary/Keyword: Live Fish

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Analysis and Classification of Broadband Acoustic Echoes from Individual Live Fish using the Pulse Compression Technique (펄스압축기법을 이용한 활어 개체어에 대한 광대역 음향산란신호의 분석 및 식별)

  • Lee, Dae-Jae;Kang, Hee-Young;Kwak, Min Son
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.2
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    • pp.207-220
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    • 2015
  • This study identified the species-specific, frequency-dependent characteristics of broadband acoustic scattering that facilitate classifying fish species using the pulse compression (PC) technique. Controlled acoustic scattering laboratory experiments were conducted with nine commercially important fish species using linear chirp signals (95-220 kHz) over an orientation angle range of ${\pm}45^{\circ}$ in the dorsal plane at approximately $1^{\circ}$ increments. The results suggest that the angular-dependent characteristics of the broadband echoes and the frequency-dependent variability in target strength (TS) were useful for inferring the fish species of interest. The scattering patterns in the compressed pulse output were extremely complex due to morphological differences among fish species, but the x-ray images strongly suggested that spatial separation correlated well with scattering for the head, skeleton, bone, otoliths, and swim bladder within each specimen.

Fish length dependence of target strength for black porgy and fat greenling at two frequencies of 70 and 120kHz (70 및 120kHz에서 쥐노래미와 감성돔에 대한 음향 반사 강도의 체장 의존성)

  • Lee, Dae-Jae
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.48 no.2
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    • pp.137-146
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    • 2012
  • Black porgy and fat greenling are commercially important fish species due to the continuously increasing demand in Korea. When estimating acoustically the fish length by a fish sizing echo sounder, it is of crucial importance to know the target strength (TS) to length dependence. In relation to these needs, the target strength experiments for live fishes were conducted in an acrylic salt water tank using two split-beam echo sounders operating at 70 and 120kHz. The target strength under well-controlled laboratory conditions was simultaneously measured with the swimming movement by digital video recording (DVR) system and analyzed as a function of fish length (L) and frequency (or wavelength ${\lambda}$). Equations of the form TS-alog (L)+blog (1)+c were derived for their TS-length dependence. The best fit regression of TS on fork length for black porgy was TS=20.62 log (L, m)-0.62 log (${\lambda}$, m)-30.68 ($r^2$=0.77). The best fit regression of TS on fork length for fat greenling was TS=12.06 log (L, m)-5.85 log (${\lambda}$, m)-22.15 ($r^2$=0.44).

Visualization of chromatin higher-order structures and dynamics in live cells

  • Park, Tae Lim;Lee, YigJi;Cho, Won-Ki
    • BMB Reports
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    • v.54 no.10
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    • pp.489-496
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    • 2021
  • Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.

Residues of Antibiotics in Wild and Cultured Fishes Collected from Coast of Korea (시중 유통 자연산 및 양식산 활어의 항생제 잔류)

  • Shim, Kil-Bo;Mok, Jong-Soo;Jo, Mi-Ra;Kim, Poong-Ho;Lee, Tae-Seek;Kim, Ji-Hoe;Cho, Young-Je
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.1
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    • pp.12-17
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    • 2010
  • Wild and cultured fish including olive flounder, sea bass, rock bream, yellowtail, gray mullet, gizzard shad, black rockfish, red seabream and squid were collected from a fish market located on the coast of Korea, and the antibiotic content of their muscle was investigated. Tetracycline group antibiotics were not detected in the 108 individuals of 9 species of wild fish. However, oxytetracycline (OTC) and tetracycline(TC) were detected in some samples of the 111 individuals in 7 cultured live fish species. The detected ranges of OTC and TC were ND~ 0.06 and ND~ 0.03, respectively. Five different fluoroquinolone antibiotics were also tested for, but were not detected in the wild fish species. Only small amount of criprofloxacin(ND~0.029 mg/kg) were detected in a few cultured fish samples. Oxolinic acid was not detected in either wild and cultured fish samples. Results showed that even very low levels of antibiotics could be detected by the testing methods used. Antibiotics were identified in a few fish samples but levels were far below the maximum allowable limits of the Korean Food Code, and the safety of fish being sold in markets, with regard to antibiotic levels, was confirmed.

An Overview of Kenyan Aquaculture: Current Status, Challenges, and Opportunities for Future Development

  • Munguti, Jonathan Mbonge;Kim, Jeong-Dae;Ogello, Erick Ochieng
    • Fisheries and Aquatic Sciences
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    • v.17 no.1
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    • pp.1-11
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    • 2014
  • The Kenyan aquaculture sector is broadly categorized into freshwater aquaculture and mariculture. Whereas freshwater aquaculture has recorded significant progress over the last decade, the mariculture sector has yet to be fully exploited. The Kenyan aquaculture industry has seen slow growth for decades until recently, when the government-funded Economic Stimulus Program increased fish farming nationwide. Thus far, the program has facilitated the alleviation of poverty, spurred regional development, and led to increased commercial thinking among Kenyan fish farmers. Indeed, national aquaculture production grew from 1,000 MT/y in 2000 (equivalent to 1% of national fish production) to 12,000 MT/y, representing 7% of the national harvest, in 2010. The production is projected to hit 20,000 MT/y, representing 10% of total production and valued at USD 22.5 million over the next 5 years. The dominant aquaculture systems in Kenya include earthen and lined ponds, dams, and tanks distributed across the country. The most commonly farmed fish species are Nile tilapia Oreochromis niloticus, which accounts for about 75% of production, followed by African catfish Clarias gariepinus, which contributes about 21% of aquaculture production. Other species include common carp Cyprinus carpio, rainbow trout Oncorhynchus mykiss, koi carp Cyprinus carpio carpio, and goldfish Carassius auratus. Recently, Kenyan researchers have begun culturing native fish species such as Labeo victorianus and Labeo cylindricus at the National Aquaculture Research Development and Training Centre in Sagana. Apart from limited knowledge of modern aquaculture technology, the Kenyan aquaculture sector still suffers from an inadequate supply of certified quality seed fish and feed, incomprehensive aquaculture policy, and low funding for research. Glaring opportunities in the Kenyan aquaculture industry include the production of live fish food, e.g., Artemia, daphnia and rotifers, marine fish and shellfish larviculture; seaweed farming; cage culture; integrated fish farming; culture of indigenous fish species; and investment in the fish feed industry.

Fish length dependence of acoustic target strength for 12 dominant fish species caught in the Korean waters at 75 kHz (한국 연근해에서 어획된 주요 12어종의 75 kHz에 대한 음향 반사 강도의 체장 의존성)

  • Lee, Dae-Jae
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.41 no.4
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    • pp.296-305
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    • 2005
  • Acoustic target strength (TS) of 12 commercially important fish species caught in the Korean waters had been investigated and their results were presented. Laboratory measurements of target strength on 12 dominant fish species were carried out at a frequencies of 75 kHz by single beam method under the controlled condition of the water tank with the 241 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N ($-5.0^{\circ}$, $^15.0{\circ}$). The 75 to fish length relationship for each species was independently derived by a least - squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to fish length and fish species. An empirical model for fish target strength(TS, dB) averaged over the dorsal aspect of 158 fishes of 7 species and which spans the fish length(L, m) to wavelength(${\lambda}$, m) ratio between 6.2 and 21.3 was derived: TS: 27.03 Log(L)-7.7Log(${\kanbda}$)-17.21, ($r^2$=0.59).

Development of a Fish-trait Editor for Constructing Cyber Aquarium (사이버 아쿠아리움 구축을 위한 어류속성 편집기 개발)

  • Kang, Gyeong-Heon;Geong, Seung-Moon;Lee, Hyeon-Cheol;Kim, Eun-Seok;Hur, Gi-Taek
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.528-532
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    • 2006
  • In a cyber aquarium, the behavior of fish objects is the most important factor in respect of visual effects. In this paper, we analyze the principal habits of fish objects such as the vitality, the range of movement, the maximum depth of water they can live, and the cycle of eating. Then, we suggest a method for simulating the stable marine ecosystem with controlling the behaviors, the interaction with other species, the average span of life, and all that sort of thing based on the result of analysis. Because we can freely modify the behavior of fish object by altering the values of attributes, it can be utilized in the dynamic cyber aquarium, the 3D aquarium screen saver, and the cyber fish game, and so on.

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Comparison of immunogenecities of three beta-nodavirus proteins, capsid protein, non-structural protein B1 and B2 in olive flounder

  • Cha, Seung-Ju;Do, Jeong-Wan;Ko, Myoung-Seok;Kim, Jin-Woo;Park, Jeong-Woo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.219-228
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    • 2009
  • The genomic and subgenomic RNAs of fish nodavirus encode the four proteins, protein A, capsid protein, non-structural protein B1 and B2. In this study, we describe the immune response of olive flounder Paralichthys olivaceus immunized with live fish nodavirus or recombinant capsid protein, non-structural protein B1 and B2 expressed in E. coli. Nodavirus-infected flounder produced antibodies to capsid protein, B1 and B2 and nodavirus-neutralizing activities were detected in the serum of the nodavirus-infected flounder. The flounder were immunized against the three recombinant proteins of fish nodavirus and the sera from these immunized fishes were assayed for nodavirus-specific antibody by ELISA and a neutralization test. In the immunized flounder, all three recombinant proteins induced the production of similar levels of antibody, but only the antibody to capsid protein significantly neutralized nodavirus. These results indicate that all three nodaviral proteins are immunogenic in flounder, but only the capsid protein can induce neutralizing antibody against nodavirus.

Species composition of the demersal fish assemblage in the coastal waters off Geomun island, Korea (거문도 주변 해역에 출현하는 저어류 군집의 종조성)

  • Kim, Sin-Gon;Jang, Choong-Sik;An, Young-Su;Koh, Eun-Hye;Baeck, Gun-Wook
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.50 no.2
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    • pp.131-138
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    • 2014
  • The study aims at analyzing a species composition of the fish live in bottom of the coastal waters off Geomun island, Korea. To reach the goal of the study, a species composition and it's monthly fluctuation, a monthly fluctuation of the dominant species and it's appearance type was analyzed the fishes caught by the bottom trawl. The result obtained can be summerrized as follows; Fish caught in this area composes 14 orders, 34 families, and 47 species. The largest number of fish species was Perciformes (15 families and 19 species). The most dominant species in an number of fish was jhon dory, Zeus faber which was occupied at 22.5% from the whole number, the second most dominant species was horse mackerel, Trachurus japonicus which was occupied at 21.2%. Four groups were categorized based on appearance time period.