• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2000.10a
• /
• pp.58-59
• /
• 2000

최근들어 연근해 어장의 황폐화로 일시 다획성 어종의 어획량이 줄어들고 있을 뿐만 아니라, 배타적 경제수역 선포로 연근해 어장의 상실에 따른 어획량의 감소가 예상되고 있다. 현행의 해수와 육상빙을 50:50으로 하여 어획물을 저장 운반하는 방법인 수빙식은 선도 유지가 잘되지 않으므로 단백질 자원의 고도이용 및 국민건강 증진을 위해서는 어획된 생선을 운반시에 운반선에 적절한 선도관리 장치를 설치하여 선도저하를 억제시켜야 한다. (중략)

• Journal of Chest Surgery
• /
• v.26 no.1
• /
• pp.67-70
• /
• 1993

Asian countries including Korea, Japan, China, Taiwan, and Philippines are endemic area of Paragonimiasis as people who live those countries have special diet habbit to ingest raw fish and meat. Of the Paragonimus westermani infestation, mediastinal paragonimiasis is a rare condition because of its life cycle. We experienced a case of mediastinal parasitic cyst resected under video-thoracoscopy, which was confirmed as paragonimiasis granuloma postoperativley.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 2003.10b
• /
• pp.121-121
• /
• 2003

Cricket (Gryllus bimacutus) is mass-bred in 6 cycles per one year in insect farms. They are used as dry or live foods for animals, tropical fish, reptile and amphibians. Therefore, it is necessary to study the genotoxicity of whole bodies of G. bimaculatus.(omitted)

• Journal of fish pathology
• /
• v.19 no.2
• /
• pp.173-181
• /
• 2006

Miamiensis avidus is a scuticociliate causing mortality in olive flounder Paralichthys olivaceus. To evaluate immune response of olive flounder against M. avidus, 2.6×106cells/fish of Formalin killed cell (FKC) was intraperitoneally (i.p.) injected, and 2.4 × 106cells/㎖ of sonicated FKC was immersion immunized to 14.9 cm (26.8g) fish. Fish were immunized 2 times with 2 weeks intervals. Antiserum from immunized fish caused agglutination and immobilization of the ciliate. In ELISA test, immunized group exhibited higher titers than control group. In addition, i.p. immunized fish showed higher phagocytic ratio, phahgocytic index and chemotaxic activity. To evaluate in vivo efficacy of FKC on fish, 1.43 × 105cells/fish was i.p.. injected, and 2.2 × 105 cells/㎖ of sonicated cell was immersed into 8.6cm (6.3g) fish. After 2 times immunization with 2 weeks intervals, fish were infected with 2.0 × 104 and 2.0 × 103 cells/ml of live ciliates by immersion. After 3 weeks, cumulative mortality was lower in the i.p. immunized group and mortality was delayed in immersion immunized group. In conclusion, specific immune response of oliver flounder against M. avidus was elevated after immunization and these immune response may prevent and/or delay for the M. avidus infection to olive flounder.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.51 no.4
• /
• pp.630-636
• /
• 2015

The study aims at analyzing a species composition of the fish live in bottom of the coastal waters off Sejon island, Korea. To reach the goal of the study, a species composition and it's seasonally fluctuation, a seasonally fluctuation of the dominant species and it's appearance type were analyzed with the fishes caught by the bottom trawl in the coastal waters off Sejon island from May, 2011 to March, 2012. The results obtained can be summarized as follows; Fish caught in this area composes 10 orders, 25 families, and 37 species. The most dominant species in an number of fish was Pennahia argentata which was occupied at 42.1% from the whole number, the second most dominant species was Konosirus punctatus which was occupied at 14.2%. The most dominant species in a biomass of fish was Pennahia argentata which was 31.3% out of the whole catch, the next dominant species was Lophius litulonwhich which was 18.2%. The first group (Trichiurus lepturus, Zeus faber, Pennahia argentata, Chelidonichthys spinosus and Lophius litulon) was appeared at May, August, October, the second group (Sphyraena pinguis) was appeared at May and November, the third group (Pampus argenteus) was appeared at August, the fourth group (Pampus echinogaster, Leiognathus nuchalis, Konosirus punctatus and Engraulis japonicus) was appeared at March.

• Journal of fish pathology
• /
• v.16 no.1
• /
• pp.13-21
• /
• 2003

The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during the year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball or brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started from the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7cm in total length.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.46 no.3
• /
• pp.239-247
• /
• 2010

Species of fish such as striped beakperch, bluefin searobin and konoshiro gizzard shad are commercially very important due to their high demand in the Korean market. When estimating acoustically the abundance of stocks for these species, it is of crucial importance to know the target strength (TS) to the length dependence. In relation to these needs, the TS experiments were conducted on three different species in an acrylic salt water tank using two split-beam echo sounders of 70 and 120 kHz. The TS for these three species under the controlled condition was simultaneously measured with the swimming movement by a DVR system and analyzed as a function of fish length (L) and frequency (or wavelength $\lambda$). The equation of the form TS=a log (L)+b log ($\lambda$)+c was derived for their TS-length dependence. The best fit regression of TS on fork length for striped beakperch was estimated as TS=35.67 log (L, m) -15.67 log ($\lambda$, m) -46.69 ($r^2$=0.78). Furthermore, the best fit regression of TS on fork length for konoshiro gizzard shad was shown to be TS=25.85 log (L, m) -5.85 log ($\lambda$, m) -32.22 ($r^2$=0.51). The averaged TS for 12 bluefin searobins with a mean length of 24.36cm at 70 kHz was analyzed to be -41.55dB. In addition, the averaged tilt angle obtained simultaneously by a DVR system with TS measurements for 27 konoshiro gizzard shads swimming within an acrylic salt water tank was estimated at $-2.7^{\circ}$.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.46 no.2
• /
• pp.157-163
• /
• 2010

The indirect target strength (TS) estimation method which uses the Kirchhoff ray mode model (KRM model) was discussed to apply for a biomass estimation in the water of mixed species. TS of 25 live scorpion fishes for 120kHz were measured by a tethered method and of others dominant 5 species in the marine ranching ground of Jeju coastal water including a scorpion fish were also estimated by KRM model. The measurement TS of scorpion fish well agreed with the theoretical values and the standard formula of scorpion fish was estimated as $TS_{120kHz}=20Log\;(L)-72.9$ ($r^2=0.67$). TScm values estimated on trial to each sample of dominant 5 species were from -69.3dB to -75.1dB at 120kHz and they were in the general range of swimbladdered fish. It was clarified that TS by KRM model can be used to estimate fish biomass estimation by increasing a sample number and is more effective under the condition that there is rare TS information for inhabiting species in mixed-species area.

• Journal of fish pathology
• /
• v.37 no.1
• /
• pp.49-60
• /
• 2024

A viable but non-culturable (VBNC) state is a survival strategy adopted by bacteria when faced with unfavorable environmental conditions, rendering them unable to grow on nutrient agar while maintaining low metabolic activity. This study explored the impact of temperature and nutrient availability on inducing VBNC state in Edwardsiella piscicida, the most important bacterial fish pathogen, and assessed its pathogenicity at VBNC state. E. piscicida was suspended in filtered sterile seawater and exposed to three different temperatures (4, 10, and 25℃) to induce the VBNC state. Subsequently, the induced VBNC cells were subjected to resuscitation by either raising the temperature to 28℃ or inoculating them in brain heart infusion broth supplemented with 1% NaCl. A propidium monoazide (PMA)-qPCR method was also developed to selectively quantify live (VBNC or culturable) E. piscicida cells. The results showed that the bacteria entered the VBNC state after approximately 1 month at 4℃ and 25℃, and 2 months at 10℃. The VBNC E. piscicida cells were successfully revived within 3 days in a nutrient-rich environment at 28℃, highlighting the significance of temperature and nutrition in inducing and resuscitating the VBNC state. In pathogenicity tests, resuscitated E. piscicida cells exhibited high pathogenicity in olive flounder comparable to cultured bacteria, while VBNC cells showed no signs of infection, suggesting they are unlikely to resuscitate in fish. In conclusion, this study contributes to our understanding of fish pathogen ecology by investigating the characteristics of the VBNC state under varying temperature and nutrition conditions.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.45 no.5
• /
• pp.430-437
• /
• 2012

The effects of low temperature and starvation on the physiochemical characteristics of the muscle of the olive flounder Paralichthys olivaceus, were examined. Fish were deprived of feed for 28 days at 2, 4, 6, 8, 10, 12, and $20^{\circ}C$ in order to establish suitable conditions for live fish transportation. Throughout most of the 4 weeks of feed restriction, the physiochemical characteristics of the muscle of olive flounder were found to be dependent on the acclimation temperature. The breaking strength of muscle did not show a significant reduction during feed restriction at 2 and $4^{\circ}C$. With increasing temperatures, however, the breaking strength of muscle differed significantly according to the individual and feed restriction period (P<0.05). The moisture content in muscle acclimated at 10, 12, and $20^{\circ}C$ increased steadily over the feed-restriction period, while the crude lipid content decreased during the same period (P<0.05). At water temperatures above $2-8^{\circ}C$, no significant differences were shown during the same period. After it reached 6, 8, 10, 12, and $20^{\circ}C$, the muscle had an accumulation of lactate, and a loss of ATP. There was no change in the lactate or ATP content during the feed restriction period at 2 and $4^{\circ}C$, although there were differences among the water temperature groups. These results clearly show that temperature can have an important influence on the of muscle of physiochemical characteristics of muscle during live fish transportation.