• 산업진흥연구
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• 제8권4호
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• pp.69-79
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• 2023

본 연구는 직업스트레스가 직업적 가치를 경유하여 행복에 미치는 영향을 그릿이 조절 매개하는지를 검증하여 중국 제품디자이너의 행복 향상을 위한 방안을 제안하고자 하였다. 연구 대상으로 중국 남부 광둥성에 살고 있는 의도적으로 표집한 335명의 중국 제품 디자이너를 선정하였다. 자료 분석은 SPSS PC+ Win. Ver. 26.0과 PROCESS Macro ver. 4.2를 활용하였다. 적용한 통계기법은 빈도분석, 신뢰도분석, 상관분석, 조절된 매개효과분석이었다. 연구결과는 다음과 같다. 첫째, 직무 스트레스는 직업가치 및 그릿과 부적 상관관계를 보였으나 행복과는 유의미한 상관관계를 보이지 않았다. 직무 스트레스를 제외한 다른 변수들 간에는 정적인 유의미한 상관관계를 보였다. 둘째, 그릿이 M-SD, M, M+SD일 M+SD일때 조건부 간접효과가 유의하게 나타났다. 따라서 그릿의 조절된 매개역할이 검증되었다. 그러나 직업적 가치가 높아질수록 그릿은 행복을 높이는 역할을 하였으나, 직무스트레스->직업적 가치->행복의 경로에서는 그릿이 직업적 스트레스를 통해 행복에 미치는 부정적인 영향을 완충시키지 못했다. 직업적 가치와 그릿을 활용한 제품 디자이너들의 행복 증진을 위한 제언을 하였다.

• Asian Journal of Atmospheric Environment
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• 제6권2호
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• pp.83-95
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• 2012

China has been a top producer and exporter of refined lead products in the world since the year 2000. After the phasing-out of leaded gasoline in the late 1990s, non-ferrous metallurgy and coal combustion have been identified as potential major sources of aerosol lead in China. This paper presents the single particle analytical results of ambient aerosol particles collected near a lead smelter using a scanning electron microscopy- energy dispersive x-ray spectroscopy (SEM-EDX). Aerosol particle samples were collected over a 24-hour period, starting from 8 pm on 31 May 2002, using a high volume TSP sampler. For this near source sample, 73 particles among 377 particles analyzed (accounting for 19.4%) were lead-containing particles mixed with other species (S, Cl, K, Ca, and/or C), which probably appeared to be from a nearby lead smelter. Lead-containing particles of less than $2{\mu}m$ size in the near source sample were most frequently encountered with the relative abundances of 42%. SEM-EDX analysis of individual standard particles, such as PbO, PbS, $PbSO_4$, $PbCl_2$, and $PbCO_3$, was also performed to assist in the clear identification of lead-containing aerosol particles. Lead-containing particles were frequently associated with arsenic and zinc, indicating that the smelter had emitted those species during the non-ferrous metallurgical process. The frequently encountered particles following the lead-containing particles were mineral dust particles, such as aluminosilicates (denoted as AlSi), $SiO_2$, and $CaCO_3$. Nitrate- and sulfate-containing particles were encountered frequently in $2-4{\mu}m$ size range, and existed mostly in the forms of $Ca(NO_3,SO_4)/C$, $(Mg,Ca)SO_4/C$, and $AlSi+(NO_3,SO_4)$. Particles containing metals (e.g., Fe, Cu, and As) in this near source sample had relative abundances of approximately 10%. Although the airborne particles collected near the lead smelter contained elevated levels of lead, other types of particles, such as $CaCO_3$-containing, carbonaceous, metal-containing, nitrates, sulfates, and fly-ash particles, showed the unique signatures of samples influenced by emissions from the lead smelter.

• Geomechanics and Engineering
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• 제23권2호
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• pp.151-163
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• 2020

Grouting method is an effective way of reinforcing cracked rock masses and plugging water gushing. Current grouting diffusion models are generally developed for horizontal cracks, which is contradictory to the fact that the crack generally occurs in rock masses with irregular spatial distribution characteristics in real underground environments. To solve this problem, this study selected a cement-sodium silicate slurry (C-S slurry) generally used in engineering as a fast-curing grouting material and regarded the C-S slurry as a Bingham fluid with time-varying viscosity for analysis. Based on the theory of fluid mechanics, and by simultaneously considering the deadweight of slurry and characteristics of non-uniform spatial distribution of viscosity of fast-curing grouts, a theoretical model of slurry diffusion in an oblique crack in rock masses at constant grouting rate was established. Moreover, the viscosity and pressure distribution equations in the slurry diffusion zone were deduced, thus quantifying the relationship between grouting pressure, grouting time, and slurry diffusion distance. On this basis, by using a 3-d finite element program in multi-field coupled software Comsol, the numerical simulation results were compared with theoretical calculation values, further verifying the effectiveness of the theoretical model. In addition, through the analysis of two engineering case studies, the theoretical calculations and measured slurry diffusion radius were compared, to evaluate the application effects of the model in engineering practice. Finally, by using the established theoretical model, the influence of cracking in rock masses on the diffusion characteristics of slurry was analysed. The results demonstrate that the inclination angle of the crack in rock masses and azimuth angle of slurry diffusion affect slurry diffusion characteristics. More attention should be paid to the actual grouting process. The results can provide references for determining grouting parameters of fast-curing grouts in engineering practice.

• Journal of Ginseng Research
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• 제44권3호
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• pp.475-482
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• 2020

Background: Active natural ingredients, especially small molecules, have recently received wide attention as modifiers used to treat neurodegenerative disease by promoting neurogenic regeneration of neural stem cell (NSC) in situ. 20(S)-protopanaxadiol (PPD), one of the bioactive ingredients in ginseng, possesses neuroprotective properties. However, the effect of PPD on NSC proliferation and differentiation and its mechanism of action are incompletely understood. Methods: In this study, we investigated the impact of PPD on NSC proliferation and neuronal lineage differentiation through activation of the Wnt/glycogen synthase kinase (GSK)-3β/β-catenin pathway. NSC migration and proliferation were investigated by neurosphere assay, Cell Counting Kit-8 assay, and EdU assay. NSC differentiation was analyzed by Western blot and immunofluorescence staining. Involvement of the Wnt/GSK3β/β-catenin pathway was examined by molecular simulation and Western blot and verified using gene transfection. Results: PPD significantly promoted neural migration and induced a significant increase in NSC proliferation in a time- and dose-dependent manner. Furthermore, a remarkable increase in anti-microtubule-associated protein 2 expression and decrease in nestin protein expression were induced by PPD. During the differentiation process, PPD targeted and stimulated the phosphorylation of GSK-3β at Ser9 and the active forms of β-catenin, resulting in activation of the Wnt/GSK-3β/β-catenin pathway. Transfection of NSCs with a constitutively active GSK-3β mutant at S9A significantly hampered the proliferation and neural differentiation mediated by PPD. Conclusion: PPD promotes NSC proliferation and neural differentiation in vitro via activation of the Wnt/GSK-3β/β-catenin pathway by targeting GSK-3β, potentially having great significance for the treatment of neurodegenerative diseases.

• 한국콘텐츠학회논문지
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• 제20권4호
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• pp.212-229
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• 2020

최근 중국에서는 생방송을 이용한 마케팅 활동이 적극적으로 진행되고 있는데, 그 중에서 '생방송+판매' 형식이 크게 주목받고 있다. 왕홍은 생방송을 통해 시청자에게 제품을 설명하고 온라인 쇼핑을 촉진하며 충동구매를 일으킨다. 본 연구는 이러한 왕홍이 진행하는 판매 생방송 과정에서 왕홍 특성 중 어떤 요인이 시청자의 충동구매에 영향을 미치는지에 대해 조사하였다. 또한 이러한 과정에서 판매 생방송에 대한 플로우 경험과 시청만족도가 매개 효과를 나타내는지 알아보았다. 나아가, 판매 생방송이 진행되는 판매 환경에 따라 플로우 경험, 시청만족도, 충동 구매 행위가 조절될 것이라고 예상하였다. 이를 위해 판매 생방송 시청 경험이 있는 중국 여성 242명을 대상으로 설문조사를 실시하였다. 연구 결과, 왕홍의 신뢰성과 매력성이 시청자의 충동구매에 영향을 미치는 것으로 나타났으며, 이 과정에서 즐거움, 주의 집중, 시간 왜곡 플로우 경험, 그리고 시청만족도가 매개 효과를 나타냈다. 해외 현장에서 진행하는 방송과 중국 스튜디오에서 진행하는 방송은 유의한 차이를 나타내지 않았다. 본 연구는 판매 생방송에 따른 충동적인 구매행위를 연구함으로써 새로운 마케팅 전략인 판매 생방송에 대한 이해를 높일 수 있다는 점에서 의의를 갖는다.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.71-77
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• 2010

The time, yield, and related genes expression of PHB accumulation of Acidiphilium cryptum DX1-1 were investigated under four different initial C/N ratios, 1.2, 2.4, 7.5, and 24. The results of time and yield of poly-$\beta$-hydroxybutyrate (PHB) accumulation show that the initial C/N ratio of 2.4 was optimum for strain DX1-1 to accumulate PHB, but both higher and lower initial C/N ratios did not favor that process. Based on the genome of Acidiphilium cryptum JF-5, 13 PHB accumulation related genes in strain JF-5 were chosen and successfully cloned from strain DX1-1. The differential expressions of the 13 functional genes, in different C/N ratios as cited above, were then studied by real-time PCR. The results show that all the 13 genes were most upregulated when the initial C/N ratio was 2.4, and among which the gene Acry_3030 encoding poly-$\beta$-hydroxybutyrate polymerase and Aery_0626 encoding acetyl-CoA synthetase were much more upregulated than the other genes, which proved that they play the most important role for PHB accumulation, and acetate is the main initial substance for PHB accumulation for strain DX1-1. Potential regulatory motifs analysis showed that the genes related to PHB accumulation are regulated by different promoters and that the motif had weak similarity to the model promoters, suggesting that PHB metabolism in Acidiphilium cryptum may be mediated by a different mechanism.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1069-1077
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• 2009

Castration of male pig produces significant negative effects on skeletal muscle development. The androgen receptor (AR), two splice variants of insulin-like growth factor-I (IGF-I Ea and MGF) and the myostatin gene may play important roles in this process. In the present study, the expression of AR, IGF-I Ea, MGF and myostatin genes in three skeletal muscles, the brachialis, longissimus and semitendinosus, were studied using real-time quantitative RT-PCR. Our experimental design used 14 pairs of male Landrace sire${\times}$Yorkshire dam piglets. The two piglets in each pair were full sibs, one of which was castrated at 21 d of age; the other remained intact. The study group was divided into subgroups of equal size. Animals in the first subgroup were slaughtered at 147 d and those of the second at 210 d of age. Carcass weight and lean meat yield were similar between boars and barrows at 147 d of age (p>0.05), whereas barrows had lower carcass weight and less lean meat yield at 210 d of age (p<0.05). Castration caused down-regulation of AR gene expression at both 147 and 210 d of age (p<0.05). The two splice variants of the IGF-I gene from porcine skeletal muscle were cloned using RT-PCR, and it was found that MGF differs from IGF-I Ea in having a 52-base insert in the last coding exon of the mRNA. Both splice variants were down-regulated by castration only at 210 d of age (p<0.05). No differences in expression of the myostatin gene were observed between boars and barrows at either 147 or 210 d of age (p>0.05). These results suggest that the downregulation of AR, IGF-I Ea and MGF gene expression following castration helps to explain the negative effect of castration on skeletal muscle development.

• 한국콘텐츠학회논문지
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• 제20권7호
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• pp.302-315
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• 2020

환경설계를 통한 범죄예방이론(Crime Prevention Through Environmental Design : CPTED)은 어떻게 지속 가능한 발전의 수요에 따라 융합할 것인가라는 문제에 있어서 아직 현단계 제3세대 이론의 발전 과정을 지속하고 있다. 게다가 국제적으로도 논쟁과 토론을 많이 일으키고 있다. 더욱 주목할 만한 것은 그 논쟁과 토론하는 관점이 이론 모델 및 이론 모델 속의 원칙 간의 상호 작용 관계에 무시 못 할 영향을 일으켰다는 것이다. 본 연구는 이론 발전의 전체적 맥락을 파악한 후 CPTED이론 발전의 정체성이 환경과 환경 사용자 상호 간에 다양한 이해의 차원과 교류의 방식을 제공한다. CPTED 이론 발전의 정체성을 기초로 해서 CPTED 범죄 예방 디자인 패턴은 지속 가능성의 다양한 차원을 연결시켜야 하며, 거주적합성을 디자인 목표의 출발점으로 삼고 Top-down 패턴에 의거하여 환경의 안전성에 대해 조정해야 한다는 관점을 명확히 했다. 그러나 단지 기존 물리적 설계 방법으로 상향식(Bottom-up)의 패턴을 사용해 환경을 개선해서는 안 된다. 그래서 본 연구는 정체성을 핵심으로 하향식의 범죄 예방 디자인 패턴을 결합해, 이를 이론 모델의 재구성으로 시각화 제안을 제기했다. 이는 어떤 단계의 성과나 원칙의 응용에 보충하는 것이 아니며, 따라서 CPTED의 이론적 모델과 지속 가능한 발전에 참고와 자료를 제공할 것으로 기대된다.

• 한국건설관리학회논문집
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• 제16권1호
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• pp.82-91
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• 2015

최근 전 세계적으로 친환경 및 저탄소에 대한 관심이 증가하고 있으며, 이에 따라 대표적인 친환경연료인 천연가스의 사용량이 급속도로 증가하고 있다. 특히 국내에서도 산업용, 발전용, 가정용으로 사용되는 천연가스 수요증가에 대비하여 도입 물량을 확대하기 위한 전략을 수행하고 있으며, 이에 따른 LNG 저장용량의 확보를 위해 저장기지 증설을 계획하고 있다. 그러나 기존의 LNG 저장기지의 입지선정은 기업의 내부적인 절차나 용역을 통해 수행되어 LNG 및 LNG 저장기지의 특성을 반영하는 데는 미흡하였다. 또한 해외에서도 LNG 저장기지의 입지선정과 관련하여 주요 요인들에 대한 연구가 수행되고 있으나 프로세스 및 모델 등 체계적인 분석은 미흡한 상황이다. 따라서 본 연구에서는 LNG 저장기지의 특성을 고려한 입지선정 모델을 구축하고자 한다. 이를 위해 관련기업의 과거 사례를 분석하여 저장기지 입지선정 과정에서 요구되는 요인들과 기존에 연구되어온 플랜트시설, 공장, 산업단지, 관청청사 등의 입지선정에 대한 요인들을 취합하여 전문가 인터뷰를 실시하였고 최종적으로 47개의 입지선정요인을 도출하였다. 이후 기 수행된 5개 지역(PT지역, IC지역, TY지역, SC지역, BR지역)의 사례에 대한 설문을 기반으로 요인분석, 다중회귀분석을 통하여 지역별 입지선정에 대한 우선순위를 도출하였고 이를 토대로 LNG 저장기지 후보지에 대한 입지선정 모델의 활용 가능성을 검토하였다. 향후 LNG 저장기지의 추가 건설과정에서 본 연구를 기초자료로서 활용한다면, 보다 효과적이고 체계적인 입지선정이 가능할 것으로 기대된다.

• BMB Reports
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• 제41권4호
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• pp.287-293
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• 2008

In a yeast two-hybrid screen, we identified the microtubule-destabilizing protein SCG10 as a potential effector protein of $BRI_3$. The association was verified using GST pull-down, Co-IP, and their perinuclear co-localization. The analysis of in vitro microtubule polymerization/depolymerization showed that the binding of $BRI_3$ to SCG10 effectively blocked the ability of SCG10 to induce microtubule disassembly, as determined by turbidimetric assays. In intact PC12 cells, $BRI_3$ exhibited the ability to stabilize the microtubule network and attenuate the microtubule-destabilizing activity of SCG10. Furthermore, co-expression of $BRI_3$ with SCG10 attenuated SCG10-mediated PC12 cell neurite outgrowth induced by NGF. These results identify a novel connection between a neuron-specific BRI protein and the cytoskeletal network, suggesting possible roles of BRI3 in the process of neuronal differentiation.