• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.838-845
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• 2011

The present work describes the identification, purification, and characterization of bile salt hydrolase (BSH) from Bifidobacterium animalis subsp. lactis. The enzyme was purified to electrophoretic homogeneity by hydrophobic chromatography, ion-exchange chromatography and ultrafiltration. SDS-PAGE analysis of putative BSH and gel filtration revealed that the analyzed protein is presumably a tetramer composed of four monomers each of about 35 kDa. The purified enzyme was analyzed by liquid chromatography coupled to LTQ FT ICR mass spectrometry and unambiguously identified as a bile salt hydrolase from B. animalis. The isoelectric point of the studied protein was estimated to be around pH 4.9. The pH optimum of the purified BSH is between 4.7 to 6.5, and the temperature optimum is around 50oC. The BSH of B. animalis could deconjugate all tested bile salts, with clear preference for glycine-conjugated bile salts over taurine-conjugated forms. Genetic analysis of the bsh showed high similarity to the previously sequenced bsh gene from B. animalis and confirmed the usefulness of bile salt hydrolase as a genetic marker for B. animalis identification.

• Nuclear Engineering and Technology
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• v.51 no.4
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• pp.1024-1031
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• 2019

The Molten Salt Fast Reactor (MSFR) with its liquid circulating fuel and its fast neutron spectrum calls for a new safety approach including technological neutral methodologies and analysis tools adapted to early design phases. In the frame of the Horizon2020 program SAMOFAR (Safety Assessment of the Molten Salt Fast Reactor) a safety approach suitable for Molten Salt Reactors is being developed and applied to the MSFR. After a description of the MSFR reference design, this paper focuses on the identification of the Postulated Initiating Events (PIEs), which is a core part of the global assessment methodology. To fulfil this task, the Functional Failure Mode and Effect Analysis (FFMEA) and the Master Logic Diagram (MLD) are selected and employed separately in order to be as exhaustive as possible in the identification of the initiating events of the system. Finally, an extract of the list of PIEs, selected as the most representative events resulting from the implementation of both methods, is presented to illustrate the methodology and some of the outcomes of the methods are compared in order to highlight symbioses and differences between the MLD and the FFMEA.

• Nuclear Engineering and Technology
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• v.56 no.3
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• pp.793-802
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• 2024

In the framework of the European project SAMOSAFER, this numerical study focuses on some thermal aspects of the Emergency Draining Tank (EDT) located underneath the core of a Molten Salt Reactor. In case of an emergency, this tank passively receives the liquid fuel salt and is designed to ensure a subcritical state. An important requirement is that the fuel does not overheat to maintain the EDT Hastelloy container integrity. The present EDT is based upon a group of hexagonal cooling assemblies arranged in a hexagonal grid and cooled down thanks to conduction through the inert salt layer up to an air flow in charge of removing the heat. This numerical thermal study relies on a conjugated heat transfer analysis coupling a Finite Element solid thermal code (SYRTHES) and two instances of a Finite Volume CFD codes (Code_Saturne). Calculations on an initial design suggest that a simple center airpipe flow is likely to not sufficiently cool the device. Alternative solutions have been evaluated. Introduction of fins to enhance the heat transfer do not bring a noticeable improvement regarding maximum temperature reached. However, a solution in which the central pipe air flow is replaced by several cooling channels located closer to the fuel is investigated and suggests a better cooling.

• Bulletin of the Korean Chemical Society
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• v.28 no.6
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• pp.1060-1062
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• 2007

• Bulletin of the Korean Chemical Society
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• v.16 no.6
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• pp.489-492
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• 1995

A new synthetic route to chiral liquid crystal dopant, 4-[2-(7S-methylnonanyl)oxy-5-pyrimidinyl]phenyl(2S,3S)-2-chloro-3-methylpentanoate (1), starting from 4-nitrophenylacetic acid is described. The key intermediate methylthiopyrimidine compound (8) has been synthesized from 4-nitrophenylacetic acid by Vilsmyer-Haack reaction followed by the formation of pyrimidine ring, and then converted to chiral ester (1) by the replacement of nitro group by (2S,3S)-2-chloro-3-methylpentanoic acid 2 through the formation of diazonium salt.

• Journal of Animal Environmental Science
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• v.19 no.2
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• pp.183-190
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• 2013

Establishment of a new concept of environmental friendly livestock manure management is required based on the facts. But now liquid fertilizer quality in korea shows a large difference among regions and the regulations are uncertain. Focusing on precedent study on main level-grading factors of liquid fertilizer quality certification, the study collected laws and standards related to liquid fertilizer of livestock manure at home and abroad and produced evaluation standards. Liquid fertilizer was divided into four factors (fertilizing value, harmfulness, stability and uniformity). According to each item, scores were awarded based on 16 details: fertilizing value (Nitrogen concentration, the whole concentration of Nitrogen, Phosphoric acid and Kalium), harmfulness (heavy metals, pathogenic microorganism and antibiotics), stability (maturity degree and odour), uniformity (EC, BOD, SS, moisture content and salt). The grade of liquid fertilizer, A (42~48), B (34~41), C (26~33) were rated using total scores.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.4_2
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• pp.595-602
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• 2022

This study was conducted to investigate the characteristics of apple fermentation when different types of salt were used during apple fermentation, and when salt was added or not. The pH decreased in all experimental groups during the fermentation process, and the final pH was 4.72-3.31. The sugar content was 17.8-18.5 °Brix, the lowest in the sea salt (FAS), and the highest in the control group (FA). The acidity was measured as 0.48-0.56%, which was high in the control group (FA), and was 0.83-1.06% on the 7th day of final fermentation. The alcohol content was generated from the 5th day of fermentation, and the control group (FA) showed a distribution of 39.23-29.4%, and the alcohol content was higher in the control group than in the experimental group, As for the total polyphenol content and total DPPH content, it was confirmed that the purified salt (3.11 mg GAE/mL) showed the highest total polyphenol content than the control (2.25 mg GAE/mL). When salt was added, the fermentation progress was excellent, and when the salt concentration was high, it was confirmed that the possibility of apple fermentation was high.

• Proceedings of the KIEE Conference
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• 2001.11b
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• pp.377-379
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• 2001

Contamination flashover is responsible for insulator electrical failures. Particularly, in Korea, with its perennially dry spring, the first spring rain often cause serious line outages by forming a conductive liquid film on the insulator surface. Rainwater and fog are not normally conductive but unfortunately atmospheric dust deposited on the insulator surface contains soluble salts which may lead to bad condition of insulation by combining watery and salts. Transmission design engineers have used a contamination map drawn on the traditional paper map. But it is not convenient because it does not include the information of Geographic Information accurately. This paper explains the newly developed salt contamination map program using Geographic Information System, which provide accurate geographic information. The program is designed to use four parts of datum, salt contamination levels, 345kV & 154 kV transmission lines, power plants & substations and background map. The digital background map is composed of raster files, the others are done by vector map.

• Membrane and Water Treatment
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• v.2 no.4
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• pp.267-279
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• 2011

At steady state, the simultaneous transport of sulphuric acid and nickel sulphate through an anion-exchange membrane Neosepta-AFN (Astom Corporation, Tokyo, Japan) was investigated in a two-compartment counter-current dialyzer with single passes. The transport was quantified by the recovery yield of acid, rejection of salt and four phenomenological coefficients, which were correlated with the acid and salt concentrations in the feed. The phenomenological coefficients were determined by the numerical integration of the basic differential equations describing the concentration profiles of the components in the dialyzer. This integration was combined with an optimizing procedure. The experiments proved that the acid recovery yield is in the limits from 63 to 91 %, while salt rejection is in the limits from 79 to 97 % in the dependence on the volumetric liquid flow rate and composition of the feed.

• Food Science and Industry
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• v.53 no.2
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• pp.200-214
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• 2020

The traditional Korean fermented fish products are classified into mainly three groups; Jeot-gal, Aek-jeot, and Sik-hae. Jeot-gal is a salt-fermented fish. Aek-jeot (Eoganjang) is actually a liquid part of Jeot-gal. Sik-hae is a salt-fermented whole or part fisheries with adjuncts. The production of jeot-gal products has been increased constantly. However, there is not enough fishery for raw materials. Recently, consumers have been preferred low-salted foods because they have become aware that high levels of salt cause adult diseases such as hypertension or gastric cancers. The main consumers of jeot-gal are adults above 40~50 years old. Young generation and school nutrition teachers dislike fishery products because of distinct fish smell, small bone, as well as food safety. Therefore, in order to increase the consumption of jeot-gal and extend its industry, jeot-gal should be developed to match the preference of new generation with good safety, health-oriented, and new concept.