• 한국수자원학회논문집
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• 제51권6호
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• pp.481-490
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• 2018

최근 기후변화와 이상기후의 영향으로 인한 홍수재해의 시 공간적 패턴의 변화가 복잡해짐에 따라 홍수범람 예측은 점점 어려워지고 있다. 이러한 기상이변에 따른 홍수피해를 예방하고 대응하기 위한 비구조적 대책으로 홍수위험등급 및 범람범위 등의 정보를 포함하고 있는 홍수위험지도의 작성이 필요하다. 실제로 고정밀도 홍수위험지도를 작성하기 위해서는 1차적으로 지형, 지질, 토지피복, 기상 등의 자료를 기반으로 강우-유출-범람해석을 통해 침수면적 및 침수깊이 등 범람 정보를 획득해야 되며, 2차적으로 피해액 산정을 위해 사회 경제와 관련된 다양한 DB를 필요로 한다. 하지만 개발도상국에서는 이러한 자료가 부족하고, 일부지역에서는 자료자체를 획득할 수가 없어 홍수위험지도 제작이 불가능하거나 그 정확도가 매우 낮은 실정이다. 본 연구에서는 ASTER 또는 SRTM과 같은 범용 지형자료로부터 주요 지형학적 인자를 선정하고, 선형이진분류법(Liner binary classifiers)과 ROC분석(Receiver Operation Characteristics)을 사용하여 실제 홍수유역을 유사하게 모의하는 최적 지형학적 인자를 도출하고, 이를 기반으로 광역 홍수범람지도를 작성하는 방안을 제시한다. 본 연구에서 제시하는 방법론의 정확도 검증을 위해 북한(2007), 방글라데시(2007), 인도네시아(2010), 태국(2011), 미얀마(2015) 5개국의 대규모 홍수범람에 대해 적용하였다. 실제 홍수범람 영상정보에서 획득된 침수면적과의 공간적 비교 검토 결과, 최저(38%, 방글라데시), 최고(78%)으로 평균적으로 약 60%의 정확도를 나타내는 것으로 분석되었다. 본 연구에서 제시하는 지형학적 인자 기반의 홍수범람지도 작성방법은 미계측유역에 대해서도 DEM만을 사용하여 홍수위험 지역을 쉽게 구분할 수 있다는 장점을 가지고 있어 1 2차원 범람해석 모형의 적용이 어려운 대유역에 대해 홍수범람 우려지역에 대한 공간정보를 제공해줄 수 있을 것으로 판단된다.

• 분석과학
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• 제16권5호
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• pp.349-357
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• 2003

유전학적으로 밝혀지지 않은 생물학적 연구에 있어서 작은 분자량의 아미노산 또는 홀몬과 같은 대사체들은 그 변화와 여러 생물학적 데이타와 결합 되어 직접적인 생화학적 의미 해석을 가능하게 한다. 미량의 생체 시료에 존재하는 아미노산을 분석하기 위해서 HPLC/FLD를 사용하였으며, 감도가 우수하고 반응시간이 빠른 유도체화 방법인 OPA/3-MPA로 형광 유도체화 하여 세포 배지를 바탕시료로 하여 아미노산을 분석하였다. 유도체화물의 시간에 대한 불안정성을 개선하기 위하여 다 단계의 injector program을 사용하여 유도체화 반응 후 시료 주입시간을 일정하게 조절하여 유도체화 과정 중 발생할 수 있는 불순물 제거 및 정량성을 개선하였다. 19종 아미노산의 표준 검정 곡선은 0.5 - 100.0 ppb의 범위에서 $r^2=0.99$ 이상의 직선성을 나타냈으며, 검출 한계는 1.70 pmol(GLU) - 23.81 pmol(SER) 범위로 측정되었다. 이를 통해 다량의 세포를 대상으로 하는 대사 프로필을 위해 감도가 우수하고 안정적으로 정량할 수 있는 분석법을 설정하였다.

• Radiation Oncology Journal
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• 제23권1호
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• pp.51-60
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• 2005

목적 : 분자 표적의 선택적 억제가 방사선 세포 살상 효과를 증진시키는 것으로 알려져 있으므로 선택적 COX-2 억제제와 EGF 수용체 차단제를 HeLa 세포주에 처리한 후 방사선 효과의 상승작용을 알아보고자 하였다. 대상 및 방법 : 자궁경부암 세포주인 HeLa세포에서 EGF 수용체 차단제 AG 1478, 선택적 COX-2 억제제 NS 398과 방사선을 복합 투여하여 세포성장 억제 분석(cell graph inhibition assay)과 세포사멸 분석(apoptosis assay)을 시행하였고, 방사선 감수성 변화를 살펴보기 위해 세포생존 분석(clonogenic survival assay)을 시행하였다. 방사선 감수성 인자로는 2 Gy에서의 세포생존분획($SF_2$)과 linear-quadratic model을 이용한 dose enhancement ratio (DER)를 사용하였다. 방사선 감수성에 대한 작용기전 분석을 위해 flow cytometry로 세포주기 분석(cell cycle analysls)을 시행하였고, western blot 분석을 통하여 bcl-2와 bax 단백질의 발현 변화를 살펴보았다. 결과 : HeLa세포에 NS 398과 AG 1478을 방사선과 함께 복합 투여한 실험 군에서 세포사멸 정도가 가장 높게 나타났다($8.49\%$ vs. $22.70\%$). 세포주기 분석 결과, 방사선과 복합 약물 처리군에서 $G_0/G_l$ 세포주기 정체와 5 세포 분획 소실이 나타났으며 이러한 변화는 72시간 이후까지 지속되었다 세포생존 분석 결과로는 방사선과 AG 1478군에서 $SF_{2}0.68{\pm}0.07$, DER 1.12를 보인 반면, 방사선과 복합약물처리군에서는 $SF_{2}0.12{\pm}0.01,\;DER\;3.00$으로 나타났다. Western blot분석에서는 방사선과 복합약물처리군에서 bcl-2와 bax 단백질 발현이 모두 감소하는 양상을 보였다. 결론 : 신호전달 체계를 억제하는 분자 표적 약제인 선택적 COX-2 억제제와 EGF 수용체 차단제를 방사선과 복합투여함으로써 HeLa세포의 방사선 감수성이 증가됨을 확인하였다.

• 한국콘텐츠학회논문지
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• 제8권3호
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• pp.252-263
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• 2008

본 연구에서는 L-모멘트법에 의한 지역화 빈도분석에 따른 설계강우량 추정에 관한 연구를 수행하였다. 제주도와 울릉도의 강우관측소를 제외한 분석에 사용된 65개 강우관측소의 강우자료 수집과 선정된 강우관측지점의 강우자료의 지속시간, 즉 1, 3, 6, 12, 24, 36, 48 및 72시간 지속의 연최대치 계열을 구성하였다. 관측지점을 대상으로 Cluster분석을 실시한 결과 우리나라의 강우관측지점에 대한 합리적인 지역화로 5개의 지역으로 구분되었다. 지역화된 지역에 대한 지속기간별 극치강우자료의 적정분포모형 결정을 위한 6가지 분포모형의 적용하고 적용분포의 L-모멘트비를 산정하여 L-모멘트비도를 도시하고 K-S 검정에 의한 적정분포모형을 선정하였다. 선정된 적정분포는 GEV 분포이며 이 분포에 의해 강우관측치의 점빈도 및 지역빈도분석에 의한 설계강우량을 유도하였다. Monte Carlo 기법에 의해 모의발생된 강우량의 점빈도 및 지역빈도분석에 의한 설계강우량을 유도하였다. 실측치 및 모의발생치의 점빈도 및 지역빈도분석에 의한 설계강우량의 비교분석을 위해 상대제곱근오차와 상대편의오차에 의해 분석한 결과 점빈도 분석에 의한 설계강우량보다 지역빈도분석에 의한 설계강우량의 사용이 적정한 것으로 나타났다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1526-1526
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• 2001

To improve the accuracy of sweetness sensor in automated sorting operations, it is necessary to clarify unevenness of the sugar content distribution within fruits. And it is expected that the technique to evaluate the content distribution in fruits contribute to the development of the near-infrared (NIR) imaging spectroscopy. Sugiyama (1999) had succeeded to visualize the distribution of the sugar content on the surface of a half-cut green fresh melon. However, this method cannot be applied to red flesh melons because it depends on information of the absorption band of chlorophyll (676 nm), which is affected by the color of the fresh. The objective of this study was to develop the universal visualization method depends on the absorption band of sugar, which can be applied to various kinds of melons and other fruits. The relationship between the sugar contents and absorption spectra of both green and red fresh melons were investigated by using a NIR spectrometer to determine the absorption band of sugar. The combination of 2$\^$nd/ derivative absorbances at 902 nm and 874 nm was highly correlated with the sugar contents. The wavelength of 902 nm is attributed to the absorption band of sugar. A cooled charge-coupled device (CCD) imaging camera which has 16 bit (65536 steps) A/D resolution was equipped with rotating band-pass filter wheel and used to capture the spectral absorption images of the flesh of a vertically half-cut red fresh melon. The advantage of the high A/D resolution in this research is that each pixel of the CCD is expected to function as a detector of the NIR spectrometer for quantitative analysis. Images at 846 nm, 874 nm, 902 nm and 930 nm were acquired using this CCD camera. Then the 2$\^$nd/ derivative absorbances at 902 nm and 874 nm at each pixel were calculated using these four images. On the other hand, parts of the same melon were extracted for capturing the images and squeezed for the measurement of sugar content. Then the calibration curve between the combination of 2$\^$nd/ derivative absorbances at 902 nm and 874 nm and sugar content was developed. The calibration method based on NIR spectroscopy techniques was applied to each pixel of the images to convert the 2$\^$nd/ derivative absorbances into the Brix sugar content. Mapping the sugar content value of each pixel with linear color scale, the distribution of the sugar content was visualized. As a result of the visualization, it was quantitatively confirmed that the Brix sugar contents are low at the near of the skin and become higher towards the seeds. This result suggests that the visualization technique by the NIR imaging spectroscopy could become a new useful method fer quality evaluation of melons.

• Journal of Pharmaceutical Investigation
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• 제36권5호
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• pp.331-338
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• 2006

A rapid, selective and sensitive reverse-phase HPLC methods for the determination of atenolol and chlorthalidone in human serum and whole blood were validated, and applied to the pharmacokinetic study of atenolol and chlorthalidone combination therapy. Atenolol and an internal standard, pindolol, were extracted from human serum by liquid-liquid extraction, and analyzed on a $\mu$-Bondapak C18 $10-{\mu}$ column in a mobile phase of methanol-0.01 M potassium dihydrogenphosphate(30:70, v/v, adjusted to pH 3.5) and fluorescence detection(emission: 300 nm, excitation: 224 nm). Chlorthalidone and an internal standard, probenecid, were extracted form human whole blood by liquid-liquid extraction, and analyzed on a Luna C18 $5-{\mu}$ column in a mobile phase of acetonitrile containing 77% 0.01 M sodium acetate and UV detection at 214 nm. These analysis were performed at three different laboratories using the same quality control(QC) samples. The chromatograms showed good resolution, sensitivity, and no interference by human serum and whole blood, respectively. The methods showed linear responses over a concentration range of 10-1,000 ng/mL for atenolol and 0.05-20 ${\mu}g/mL$ for chlorthalidone, with correlation coefficients of greater than 0.999 at all the three laboratories. Intra- and inter-day assay precision and accuracy fulfilled international requirements. Stability studies(freeze-thaw, short-, long-term, extracted sample and stock solution) showed that atenolol and chlorthalidone were stable. The lower limit of quantitation of atenolol and chlorthalidone were 10 ng/mL and 0.05 ${\mu}g/mL$, respectively, which was sensitive enough for pharmacokinetic studies. These methods were applied to the pharmacokinetic study of atenolol and chlorthalidone in human volunteers following a single oral administration of Hyundai $Tenoretic^{\circledR}$ tablet(atenolol 50 mg and chlorthalidone 12.5 mg) at three different laboratories.

• Gut and Liver
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• 제12권6호
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• pp.728-735
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• 2018

Background/Aims: The combination of nab-paclitaxel and gemcitabine (nab-P/Gem) is widely used for treating metastatic pancreatic cancer (MPC). We aimed to evaluate the therapeutic outcomes and prognostic role of treatment-related peripheral neuropathy in patients with MPC treated with nab-P/Gem in clinical practice. Methods: MPC patients treated with nab-P/Gem as the first-line chemotherapy were included. All 88 Korean patients underwent at least two cycles of nab-P/Gem combination chemotherapy (125 and $1,000mg/m^2$, respectively). Treatment-related adverse events were monitored through periodic follow-ups. Overall survival and progression-free survival were estimated by the Kaplan-Meier method, and the Cox proportional hazards regression linear model was applied to assess prognostic factors. To evaluate the prognostic value of treatment-related peripheral neuropathy, the landmark point analysis was used. Results: Patients underwent a mean of $6.7{\pm}4.2$ cycles during $6.3{\pm}4.4$ months. The median overall survival and progression-free survival rates were 14.2 months (95% confidence interval [CI], 11.8 to 20.3 months) and 8.4 months (95% CI, 7.1 to 13.2 months), respectively. The disease control rate was 84.1%; a partial response and stable disease were achieved in 30 (34.1%) and 44 (50.0%) patients, respectively. Treatment-related peripheral neuropathy developed in 52 patients (59.1%), and 13 (14.8%) and 16 (18.2%) patients experienced grades 2 and 3 neuropathy, respectively. In the landmark model, at 6 months, treatment-related peripheral neuropathy did not have a significant correlation with survival (p=0.089). Conclusions: Nab-P/Gem is a reasonable choice for treating MPC, as it shows a considerable disease control rate while the treatment-related peripheral neuropathy was tolerable. The prognostic role of treatment-related neuropathy was limited.

• 한국지리정보학회지
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• 제4권2호
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• pp.27-37
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• 2001

위성영상과 GIS 공간분석기법을 이용하여 아까시나무(Robinia pseudoacasia L.) 서식지의 공간적 분포 특성을 해석하고 아까시나무 밀원식물단지의 최적지를 선정함으로서 밀원식물단지 조성을 위한 과학적인 공간분석기법의 기초를 구축하였다. 다양한 공간분석을 위하여 지형(고도, 경사, 사면방향) 수계, 토양, 임상, 토지피복 및 기상(온도, 습도, 강수량)등의 자연환경 자료와 도시, 도로 등의 인문환경 자료를 이용하였다. 선형조합법과 요소조합법을 이용한 도면중첩법 분석결과 영천시의 아카시나무 최적지는 고경, 임고, 청통, 화남면 등의 지역으로 선정되었다. 아울러 위성영상과 GIS를 이용하여 영천지역의 아까시나무 밀원단지 적지분석을 수행한 결과 생장가능지역에 대한 현재의 분포는 42.53%, 최적지 지역은 26.77%로 나타났으며 영천시 전체 면적 중 아까시나무 밀원단지 최적지 면적은 $15.79km^2$로 분석결과 산출되었다. 본 연구 결과 위성영상과 GIS 응용기법은 아까시나무 밀원식물단지 적지선정에 효과적으로 적용될 수 있을 것으로 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제17권2호
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• pp.109-129
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• 1995

Many patients with malignancies of the head and neck undergo radiation therapy, either as the only method of treatment or in combination with surgery. Radiation therapy has great effect in the case of fairly advanced malignancies which can't be operated radically. But the complication of radiation therapy arise because of damage to the peri- and operated area. It is fully known that irradiated tissue shows retarded healing process in the skin, mucosa and especially vascuslar tissue. The purpose of this study was to observe the healing process of irradiated free or island flap after operation. As Experimental Models, Femoral arterial and venous anastomosis (Group 1), Epigastric-island flap (Group 2) and free Epigastric falp(Group 3) with irradiated postoperative 24 hrs were made on 30 rats/group. As Control Model(Group 4), Free Epigastric flap was not irradiated after operation was chosen on 30 rats. The amount of irradiation was single fraction of 20 Gy using as linear megavoltage accelerator. Difference between Experimental and Control group was evaluated by the method of clinical examination, histopatholoical findings, biochemical analysis and DNA activity at postoperative 1, 3, 7, 14 and 28 days. The results were as follows, 1. Skin color and new epithelization in group 2 and 3 was similar to control group clinically. 2. Postoperarive patency of femoral artery and vien showed 5% and 22% of ischemity. 3. The externa, media and intima of irradiated femoral artery and vein were similar to control group histopathlogically. 4. Granulation and collagen tissue accumulation of irradiated groups were more active due to degenerative and fibrotic changes than control group at postoperative 7 days histopathologically. 5. The hydroxyproline content of all experimental groups were reduced till 14 days and the group 2 was most prominent at postoperative 7 and 28 days(p<0.05). 6. DNA activities of all groups were reduced till 3 days, but begun to recover at 7 days and more activities in control group than irradiated group(p<0.05). Based on the above results, the clinical healing process of free flaps with irradiated postoperative 24 hrs little difference from control group without complications.

• Ocean Systems Engineering
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• 제4권4호
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• pp.327-342
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• 2014

The tension leg platform (TLP) is one of the compliant structures which are generally used for deep water oil exploration. With respect to the horizontal degrees of freedom, it behaves like a floating structure moored by vertical tethers which are pretension due to the excess buoyancy of the platform, whereas with respect to the vertical degrees of freedom, it is stiff and resembles a fixed structure and is not allowed to float freely. In the current study, a numerical study for square TLP using modified Morison equation was carried out in the time domain with water particle kinematics using Airy's linear wave theory to investigate the effect of changing the tether tension force on the stiffness matrix of TLP's, the dynamic behavior of TLP's; and on the fatigue stresses in the cables. The effect was investigated for different parameters of the hydrodynamic forces such as wave periods, and wave heights. The numerical study takes into consideration the effect of coupling between various degrees of freedom. The stiffness of the TLP was derived from a combination of hydrostatic restoring forces and restoring forces due to cables. Nonlinear equation was solved using Newmark's beta integration method. Only uni-directional waves in the surge direction was considered in the analysis. It was found that for short wave periods (i.e., 10 sec.), the surge response consisted of small amplitude oscillations about a displaced position that is significantly dependent on tether tension force, wave height; whereas for longer wave periods, the surge response showed high amplitude oscillations that is significantly dependent on wave height, and that special attention should be given to tethers fatigue because of their high tensile static and dynamic stress.