• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1506-1512
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• 2023

Quantitative analysis of adenosine triphosphate (ATP) has been widely used as a diagnostic tool in the food and medical industries. Particularly, the pathogenesis of a few diseases including inflammatory bowel disease (IBD) is closely related to high ATP concentrations. A bioluminescent D-luciferin/luciferase system, which includes a luciferase (FLuc) from the firefly Photinus pyralis as a key component, is the most commonly used method for the detection and quantification of ATP. Here, instead of isolating FLuc produced in recombinant Escherichia coli, we aimed to develop a whole-cell biocatalyst system that does not require extraction and purification of FLuc. To this end, the gene coding for FLuc was introduced into the genome of probiotic Saccharomyces boulardii using the CRISPR/Cas9-based genome editing system. The linear relationship (r² = 0.9561) between ATP levels and bioluminescence generated from the engineered S. boulardii expressing FLuc was observed in vitro. To explore the feasibility of using the engineered S. boulardii expressing FLuc as a whole-cell biosensor to detect inflammation biomarker (i.e., ATP) in the gut, a colitis mouse model was established using dextran sodium sulfate as a colitogenic compound. Our findings demonstrated that the whole-cell biosensor can detect elevated ATP levels during gut inflammation in mice. Therefore, the simple and powerful method developed herein could be applied for non-invasive IBD diagnosis.

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2002년도 Proceedings
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• pp.119-120
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• 2002

The aim is to urge the need of elaborate commissioning of 3D RTP system from the firsthand experience. A 3D RTP system requires so much data such as beam data and patient data. Most data of radiation beam are directly transferred from a 3D dose scanning system, and some other data are input by editing. In the process inputting parameters and/or data, no error should occur. For RTP system using algorithm-bas ed-on beam-modeling, careless beam-data processing could also cause the treatment error. Beam data of 3 different qualities of photon from two linear accelerators, patient data and calculated results were commissioned. For PDD, the doses by Clarkson, convolution, superposition and fast superposition methods at 10 cm for 10${\times}$10 cm field, 100 cm SSD were compared with the measured. An error in the SCD for one quality was input by the service engineer. Whole SCD defined by a physicist is SAD plus d$\sub$max/, the value was just SAD. That resulted in increase of MU by 100${\times}$((1_d$\sub$max//SAD)$^2$-1)%. For 10${\times}$10 cm open field, 1 m SSD and at 10 cm depth in uniform medium of relative electron density (RED) 1, PDDs for 4 algorithms of dose calculation, Clarkson, convolution, superposition and fast-superposition, were compared with the measured. The calculated PDD were similar to the measured. For 10${\times}$10 cm open field, 1 m SSD and at 10 cm depth with 5 cm thick inhomogeneity of RED 0.2 under 2 cm thick RED 1 medium, PDDs for 4 algorithms were compared. PDDs ranged from 72.2% to 77.0% for 4 MV X-ray and from 90.9% to 95.6% for 6 MV X-ray. PDDs were of maximum for convolution and of minimum for superposition. For 15${\times}$15 cm symmetric wedged field, wedge factor was not constant for calculation mode, even though same geometry. The reason is that their wedge factor is considering beam hardness and ray path. Their definition requires their users to change the concept of wedge factor. RTP user should elaborately review beam data and calculation algorithm in commissioning.

• 한국연극학
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• 제53호
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• pp.167-202
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• 2014

When video composes mise-en-scene during the performance, it reflects the aspect of contemporary image culture, where the individual as creator joins in the image culture through the device of cell phone and computer remediating the former video technology. It also closely related with the contemporary theatre culture in which 1960's and 1970's video art was weaved into the contemporary performance theatre. With these cultural background, theatre practitioners regarded media-friendly mise-en-scene as an alternative facing the cultural landscape the linear representational narrative did not correspond to the present culture. Nonetheless, it can not be ignored that video in the performance theatre is remediating its historical function: to criticize the social reality. to enrich the aesthetic or emotional reality. I focused video in the performance theatre could feature the object with the image by realizing the realtime relay, emphasizing the situation within the frame, and strengthening the reality by alluding the object as a gesutre. So I explored its two historical manuel. First, video recorded the spot, communicated the information, and arose the audience's recognition of the object to its critical function. Second, video in performance theatre could redistribute perceptual way according to the editing method like as close up, slow motion, multiple perspective, montage and collage, and transformation of the image to the aesthetic function. Reminding the historical function of video in contemporary performance theatre, I analyzed two shows, Schaubuhne's Hamlet and Lenea de Sombra's Amarillo which were introduced to Korean audiences during the 2010 Seoul Theatre Olympics. It is known to us that Ostermeir found real social reality as a text and made the play the context. In this, he used video as a vehicle to penetrate the social reality through the hero's perspective. It is also noteworthy that Ostermeir understood Hamlet's dilemma as these days' young generation's propensity. They delayed action while being involved in image culture. Besides his use of video in the piece revitalized the aesthetic function of video by hypermedial perceptual method. Amarillo combined documentary theatre method with installation, physical theatre, and video relay on the spot, and activated aesthetic function with the intermediality, its interacting co-relationship between the media. In this performance theatre, video has recorded and pursued the absent presence of the real people who died or lost in the desert. At the same time it fantasized the emotional aspect of the people at the moment of their death, which would be opaque or non prominent otherwise. As a conclusion, I found the video in contemporary performance theatre visualized the rupture between the media and perform their intermediality. It attempted to disturb the transparent immediacy to invoke the spectator's perception to the theatrical situation, to open its emotional and spiritual aspect, and to remind the realities as with Schaubuhne's Hamlet and Lenea de Sombra's Amarillo.

• 지구물리와물리탐사
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• 제11권1호
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• pp.26-33
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• 2008

큰 입사각을 가진 탄성파 반사법과 굴절법 자료의 분석은 지각 규모의 구조 연구에서 중요한 역할을 한다. 그러나, 관측된 자료로부터 적합한 속도 구조 모델을 바로 얻는 것은 상당히 어려운 일이며, 지각 구조 분석은 본질적으로 비선형 문제이기 때문에 구조 모델을 단계적으로 향상 시켜야만 한다. 광각 지각 구조 모델링에는 위상식별과 시행착오 전진 모델링과 같은 몇 가지 주관적인 과정들이 있다. 광각 자료 분석에서 이러한 주관적인 과정들은 결과 모델들의 유일성과 신뢰성을 감소시키기 때문에, 분석절차에서 주관성을 감소시키는 것이 중요하다. 이러한 관점에서, 우리는 지각 구조 모델의 개발에 사용될 PASTEUP과 MODELING이라는 2개의 소프트웨어를 설명하고 있다. PASETUP은 기록 단면도의 도시, 광각 탄성파 자료 분석 그리고 위상 피킹을 쉽게 해주는 대화식 응용프로그램이다. PASETUP은 신호대잡음 비를 향상시키고 위상식별을 도와주는 분석 기능과 다양한 필터를 갖추고 있다. MODELLING은 속도모델의 편집과 파선 모델링을 위한 대화식 응용프로그램이다. MODELING에 의해 계산된 주행시간은 PASTEUP에서 관찰된 파형과 바로 비교될 수 있다. 이것은 지각구조 분석에서 가장 주관적인 과정 중 하나인 주행시간 피킹이 필요 없기 때문에 지각 구조 모델링에서 주관성을 감소시킨다. MODELING은 편집 가능한 층서구조 모델을 다중 채널 탄성파(MCS) 반사파 자료의 시간 단면도와 비교할 수 있는 왕복 주시로 변환할 수 있다. 반사파 자료와 광각 자료의 구조 모델 사이의 직접 비교는 모델에 좀 더 신뢰성을 부여한다. 게다가 PASTEUP과 MODELING 둘다 큰 자료를 다루기에 효과적인 도구이다. 이 소프트웨어들은 광각 탄성파 자료를 이용한 좀 더 그럴듯한 지각-규모의 구조 모델을 개발하는데 도움을 준다.