• 정보과학회 논문지
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• 제44권5호
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• pp.460-468
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• 2017

실시간 클라우드의 실현에 있어서 데이터 분석 프레임워크는 중추 역할을 수행한다. 현존하는 프레임워크들 중에 가장 많은 요구사항들을 충족하는 것은 Spark Streaming이다. 하지만 이 프레임워크는 초 단위 실시간 고장 복구를 충족하지 못하고 있다. Spark Streaming의 고장 복구 기법은 정상 동작시에 기록된 누적 변형 히스토리를 토대로 고장 직전 마지막 상태 데이터를 재연산하여 복구하기 때문에 히스토리의 길이에 비례하여 복구 시간이 증가된다. 따라서 제한된 시간 이내에 고장 복구가 완료됨을 보장되지 않는다. 또한 초기 상태 데이터를 고장 감내 스토리지에서 읽는 시간이 수십 초에 달하여 초 단위고장 복구 시간을 달성할 수 없다. 본 논문에서는 언급된 문제들을 해결하기 위한 두 가지 기법들을 제안한다. 이를 Spark Streaming 1.6.2에 적용하고, 실험을 통해 고장 복구 시간이 제한 시간 이내에 완료되며 평균 약 41.57% 단축됨을 확인했다.

• 한국게임학회지
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• 제6권1호
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• pp.66-69
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• 2009

지속적인 성장을 거듭하고 있는 온라인 게임은 사회적 상호작용과 집단 경쟁 등 스탠드 얼론(stand alone) 게임에서는 느낄 수 없는 새로운 재미를 제공하고 있다. 그럼에도 불구하고 온라인 게임 플레이의 경험 중가장 핵심적인 주제인 재미에 대한 연구는 휴리스틱한(heuristic) 연구에 머물고 있다. 본 연구는 기존 연구의 한계를 극복하고, 게임플레이의 재미에 대한 과학적인 평가를 위해 MMORPG의 재미 평가 모델을 제시하고, 그 유용성을 검증하는 데 목적이 있다. 본 연구는 동기이론, 몰입(Flow) 이론, 기존의 문헌연구와 설문조사를 통하여 온라인 게임의 핵심적인 재미 요소를 도출하였다. 도출된 20여개 의 요인들을 매트릭스 분석을 통해 범주화하고, Ermi & Mayra(2005)의 게임플레이 경험 모델에 통합하였다. 다양하고 정성적인 평가기준을 체계화하기 위해 AHP(Analytical Hierarchy Process) 이론에 입각하여 감각적 재미, 도전적 재미, 상상적 재미, 사회적 상호작용의 재미의 4개 측면을 포함하는 계층분석도를 구성하였고, 게임업계 전문가들의 검토와 중요도 평가를 통해 MMORPG의 재미 평가 모델을 제안하였다. 제안된 모델에 의해 구성된 설문문항을 3개 MMORPG를 즐기고 있는 이용자 87명을 대상으로 평가한 결과, WOW, Aion, Lineage2순으로 각각 75.247점, 68.649점, 62.205점을 얻어냈다. 이러한 평가 결과는 현재 MMORPG 중에서 가장 높은 시장점유율과 매출 실적이 좋은 WOW를 가장 재미있는 게임으로 선정하였고, Aion과 Lineage2도 현재의 시장 점유율과 매출 실적과도 정확하게 일치하고 있다. 이러한 결과로 본 연구에서 제안된 MMORPG의 재미 평가 모델의 유효성이 검증되었다. 본 연구의 결과는 가장 재미있는 게임의 선정뿐만 아니라 각 게임의 재미 요소별 강약점을 분석하여, 왜 어떤 게임은 성공적이며, 다른 게임들은 그렇지 않은가에 대한 이해를 제공할 수 있다. 결론적으로 본 연구에서 제안된 MMORPG의 재미 평가 모델은 어떤 게임이 가장 재미있는 게임인지를 선정하는데 활용될 수 있으며, 궁극적으로는 보다 구체적인 재미 요소별 분석을 가능하게 하여 보다 재미있는 온라인 게임을 개발하는데 기여할 것이다. 그러나 다른 장르까지 확장하여 활용하기 위해서는 후속 연구가 뒷받침되어야 한다.

• 한국어류학회지
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• 제22권4호
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• pp.273-278
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• 2010

왜매치 Abbottina springeri Banarescu and Nalbant의 분자계통학적 위치를 밝히기 위해 한국에 서식하는 버들매치속 2종과 모래주사속 5종의 핵 유전자인 recombination activating gene 1 (RAG1)의 염기서열을 분석하였다. RAG1 유전자 염기서열 정보에 기초한 계통수에서 배들매치 A. rivularis는 단계통군을 형성하는 왜매치, Biwia zezera 및 모래주사속 종들과 분리되었다. 이 계통 내에서 B. zezera는 왜매치와 모래주사속 5종을 구성된 단계통 그룹과 자매계통 관계를 보였다. 분자계통수 상에서 버들매치속 2종은 다계통군으로 나타났고, 이러한 결과는 골격 특징들에 근거한 이들의 계통적 관계를 밝힌 선행연구와 잘 일치하였다. 따라서 입의 피질돌기 유무와 부레의 골낭 유무와 크기 등과 같은 형태적 특징들에 근거한 버들배치속과 모래주사속의 현분류체계는 진화 역사를 잘 반영하지 못하는 것으로 여겨진다.

• ALGAE
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• 제33권2호
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• pp.157-166
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• 2018

Cryptic species complexes are increasingly recognized in phycological research, obscuring taxonomy and raising questions about factors influencing speciation. A recent exploration of kelp genetic diversity on Haida Gwaii, British Columbia revealed the existence of a new species, Saccharina druehlii, which is cryptic with Saccharina sessilis. This suggests that molecular investigations further north may be required to elucidate the taxonomy and evolutionary history of this lineage. Although, for several decades, S. sessilis was considered a single highly variable species, its taxonomy has been far from straightforward. In particular, Hedophyllum subsessile (Areschoug) Setchell is now recognized as a synonym of S. sessilis in North America, but as a growth form of Saccharina bongardiana in Far East Russia. To resolve this taxonomic confusion, we sequenced mitochondrial (CO1-5P) and nuclear (internal transcribed spacer) markers of S. sessilis populations from the Aleutian Islands, Alaska, USA. Interestingly, none of our sequences matched S. sessilis sensu stricto. Instead, CO1-5P sequences from populations in the central and eastern Aleutians matched exactly S. druehlii with increasing sequence divergence occurring westward. Samples from Attu, the western-most island, composed a genetic group that clearly represents Kjellman's concept of Hafgygia bongardiana f. subsessilis and is distinct enough from S. druehlii and S. sessilis to potentially constitute a distinct species. Therefore, Saccharina subsessilis comb. nov. is proposed for this entity. Our results suggest the existence of a species complex at the crown node of S. sessilis and thus further investigation of Saccharina in Alaskan waters should be conducted to reconstruct the evolutionary history of this fascinating lineage.

• Animal Systematics, Evolution and Diversity
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• 제29권2호
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• pp.99-114
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• 2013

Phylogeographic and feeding ecological studies of seven terrestrial mustelid species (Carnivora, Mustelidae), the Japanese marten Martes melampus, the sable Martes zibellina, the Japanese badger Meles anakuma, the ermine or the stoat Mustela erminea, the Japanese weasel Mustela itatsi, the least weasel Mustela nivalis, and the Siberian weasel Mustela sibirica, representing four biogeographic patterns in the Japanese archipelagos (Hokkaido, Honshu-Shikoku-Kyushu, Tsushima, and Hokkaido-Honshu), were reviewed in order to clarify causes for the faunal assemblage processes of those mustelid species in Japan. Here, three main constraints were extracted as important factors on the mustelid assemblage. First, fundamental evolutionary differences maintained by niche conservatism in each ecologically diversified lineage ("evolutionary constraint") would enable the species to co-occur without any major problem (coexistence among Martes, Meles, and Mustela species). Second, "ecological constraints" would force two closely related species to be allopatric by competitive exclusion (Mu. itatsi and Mu. sibirica) or to be sympatric by resource partitions (Mu. erminea and Mu. nivalis). Third and most importantly, "geological constraints" would allow specific species to be embraced by a particular geographic region, primarily deciding which species co-occurs. The allopatric distribution of two Martes species in Japan would have been established by the strong effect of the geological separation in Tsugaru Strait. Elucidating both phylogeny and ecology of co-existing species in a community assemblage is important to know which species possess distinct lineage and which ecological traits are adapted to local environments, fulfilling the requirement of the field of conservation biology that endemism and adaptation should both be considered. The Japanese archipelagos would, therefore, provide valuable insight into the conservation for small carnivoran species.

• International Journal of Stem Cells
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• 제7권2호
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• pp.108-117
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• 2014

Background and Objectives: Genomic imprinting is an inheritance phenomenon by which a subset of genes are expressed from one allele of two homologous chromosomes in a parent of origin-specific manner. Even though fine-tuned regulation of genomic imprinting process is essential for normal development, no other means are available to study genomic imprinting in human during embryonic development. In relation with this bottleneck, differentiation of human embryonic stem cells (hESCs) into specialized lineages may be considered as an alternative to mimic human development. Methods and Results: In this study, hESCs were differentiated into three lineage cell types to analyze temporal and spatial expression of imprinted genes. Of 19 imprinted genes examined, 15 imprinted genes showed similar transcriptional level among two hESC lines and two human induced pluripotent stem cell (hiPSC) lines. Expressional patterns of most imprinted genes were varied in progenitors and fully differentiated cells which were derived from hESCs. Also, no consistence was observed in the expression pattern of imprinted genes within an imprinting domain during in vitro differentiation of hESCs into three lineage cell types. Conclusions: Transcriptional expression of imprinted genes is regulated in a cell type- specific manner in hESCs during in vitro differentiation.

• IMMUNE NETWORK
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• 제16권1호
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• pp.1-12
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• 2016

Th2 cell immunity is required for host defense against helminths, but it is detrimental in allergic diseases in humans. Unlike Th1 cell and Th17 cell subsets, the mechanism by which dendritic cells modulate Th2 cell responses has been obscure, in part because of the inability of dendritic cells to provide IL-4, which is indispensable for Th2 cell lineage commitment. In this regard, immune cells other than dendritic cells, such as basophils and innate lymphoid cells, have been suggested as Th2 cell inducers. More recently, multiple independent researchers have shown that specialized subsets of dendritic cells mediate Th2 cell responses. This review will discuss the current understanding related to the regulation of Th2 cell responses by dendritic cells and other immune cells.

• Asian-Australasian Journal of Animal Sciences
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• 제31권3호
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• pp.439-448
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• 2018

Objective: Myogenic satellite cells were isolated from semitendinosus muscle of prepubertal Korean black goat to observe the differential effect of linolenic and retinoic acid in thepresence of thiazolidinediones (TZD) and also to observe the production insulin sensitive preadipocyte. Methods: Cells were characterized for their stemness with cluster of differentiation 34 (CD34), CD13, CD106, CD44, Vimentin surface markers using flow cytometry. Cells characterized themselves as possessing significant (p<0.05) levels of CD13, CD34, CD106, Vimentin revealing their stemness potential. Goat myogenic satellite cells also exhibited CD44, indicating that they possessed a % of stemness factors of adipose lineage apart from their inherent stemness of paxillin factors 3/7. Results: Cells during proliferation stayed absolutely and firmly within the myogenic fate without any external cues and continued to show a significant (p<0.05) fusion index % to express myogenic differentiation, myosin heavy chain, and smooth muscle actin in 2% horse serum. However, confluent myogenic satellite cells were the ones easily turning into adipogenic lineage. Intriguingly, upregulation in adipose specific genetic markers such as peroxisome proliferation-activated receptor ${\gamma}$, adiponectin, lipoprotein lipase, and CCAAT/enhancer binding protein ${\alpha}$ were observed and confirmed in all given treatments. However, the amount of adipogenesis was found to be statistically significant (p<0.01) with linolenic acid as compared to retinoic acid in combination with TZD's. Conclusion: Retinoic acid was found to produce smaller preadipocytes which have been assumed to have insulin sensitization and hence retinoic acid could be used as a potential agent to sensitize tissues to insulin in combination with TZD's to treat diabetic conditions in humans and animals in future.

• 한국식생활문화학회지
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• 제16권2호
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• pp.109-117
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• 2001

Judging from the studies carried out by Dr. Jo, Yong Jin on the Koreans' faces, Koreans divided into two constitutions according to their facial features and heritages. The one population is the Northern lineage whose ancestor migrated from Siberia in ice age. In order to survive in cold climate, they have developed a high level of metabolic heat production. Cold adaptation for preventing heat loss results in a reduction in the facial surface area with small eyes, nose and lips. The other population is the Southern lineage who is the descent of native in Korean peninsular. They have big eyes with double edged eyelids, broad nose and thick lips. It is generally believed that both genetic and environmetal factors influence eating behaviors. Although we can't recognized their heritage that may contribute to the metabolism and eating behavior, we commonly recognize their physiological heritage acceding to their facial features. In order to investigate the relationship among the size and shape of facial feature, the eating behavior, anthropometric measurement in female college students, the eating behaviors was measured during an instant-noodle lunch eaten in a laboratory setting at the ambient temperature of $23^{\circ}C$. The anterior surface area of left eye and length of right eye were positively correlated with the difference between the peak postprandial and the meal-start core temperature. The surface area of lower lip also negatively correlated with the meal-start core temperature and meal duration. In addition, the total lips' area was positively correlated with the difference between the peak postprandial and the meal-start core temperature and negatively correlated with the meal duration. However anthropometric measurements were not related with the size of facial features.

• IMMUNE NETWORK
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• 제3권2호
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• pp.89-95
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• 2003

Background: Unusually high amounts of N region addition and CDR3 length diversity were found in immunoglobulin (Ig) light chain Vk and Jk joins in patients with rheumatoid arthritis (RA). We sought to determine whether this finding is due to excessive activity of the enzyme responsible for N region addition (terminal deoxynucleotidyl transferase [TdT]) in B lineage cells in bone marrow or from positive antigenic selection of B cells with long CDR3 lengths. Methods: We used FACS to isolate $IgM^+/IgD^+$ B cells (predominantly naive) and $IgM^-/IgD^-$ B cells (predominantly class-switched) B cells from peripheral blood of a patient with RA known to have enrichment for long Vk CDR3s and from that of two normal controls. RT-PCR of VkIII transcripts was performed, followed by sequencing of individual cDNA clones. We analyzed the CDR3 lengths and N region additions in 97 clones. Results: There was enrichment for long CDR3 lengths (11 or 12 amino acids) in both $IgM^+/IgD^+$ and $IgM^-/IgD^-$ B cells in RA compared to B cell subsets in the normal controls. The $IgM^+/IgD^+$ B cell subset in RA was markedly enriched for N region addition and was similar to that seen in the $IgM^-/IgD^-$ subset. Conclusion: These data suggest that enrichment for N region addition and long CDR3 lengths in RA may result from unusually high or prolonged activity of TdT in bone marrow.