• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2251-2256
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• 2015

To study effects of cellular FLICE (FADD-like IL-$1{\beta}$-converting enzyme)-inhibitory protein (c-FLIP) inhibition by RNA interference (RNAi) on sensitivity of U2OS cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-induced apoptosis, plasmid pSUPER-c-FLIP-siRNA was constructed and then transfected into U2OS cells. A stable transfection cell clone U2OS/pSUPER-c-FLIP-siRNA was screened from the c-FLIP-siRNA transfected cells. RT-PCR and Western blotting were applied to measure the expression of c-FLIP at the levels of mRNA and protein. The results indicated that the expression of c-FLIP was significantly suppressed by the c-FLIP-siRNA in the cloned U2OS/pSUPER-c-FLIP-siRNA as compared with the control cells of U2OS/pSUPER. The cloned cell line of U2OS/pSUPER-c-FLIP-siRNA was further examined for TRAILinduced cell death and apoptosis in the presence of a pan-antagonist of inhibitor of apoptosis proteins (IAPs) AT406, with or without 4 hrs pretreatment with rocaglamide, an inhibitor of c-FLIP biosynthesis, for 24 hrs. Cell death effects and apoptosis were measured by the methods of MTT assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry, respectively. The results indicated that TRAIL-induced cell death in U2OS/pSUPER-c-FLIP-siRNA was increased compared with control cells U2OS/pSUPER in the presence or absence of AT406. Flow cytometry indicated that TRAIL-induced cell death effects proceeded through cell apoptosis pathway. However, in the presence of rocaglamide, cell death or apoptotic effects of TRAIL were similar and profound in both cell lines, suggesting that the mechanism of action for both c-FLIP-siRNA and rocaglamide was identical. We conclude that the inhibition of c-FLIP by either c-FLIP-siRNA or rocaglamide can enhance the sensitivity of U2OS to TRAIL-induced apopotosis, suggesting that inhibition of c-FLIP is a good target for anti-cancer therapy.

• Wind and Structures
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• v.34 no.2
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• pp.215-230
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• 2022

Majority of transmission line system failures at many locations worldwide have been caused by severe localized wind events in the form of tornadoes and downbursts. This study evaluates the structural response of two different transmission line systems under equivalent F2 tornadoes obtained from real incidents. Two multi-span self-supported transmission line systems are considered in the study. Nonlinear three-dimensional finite element models are developed for both systems. The finite element models simulate six spans and five towers. Computational Fluid Dynamics (CFD) simulations are used to develop the tornado wind fields. Using a proper scaling method for geometry and velocity, full-scale tornado flow fields for the Stockton, KS, 2005 and Goshen County WY, 2009 are developed and considered together with a previously developed tornado wind field. The tornado wind profiles are obtained in terms of tangential, radial, and axial velocities. The simulated tornadoes are then normalized to the maximum velocity value for F2 tornadoes in order to compare the effect of different tornadoes having an equal magnitude. The tornado wind fields are incorporated into a three-dimensional finite element model. By varying the location of the tornado relative to the transmission line systems, base shears of the tower of interest and peak internal forces in the tower members are evaluated. Sensitivity analysis is conducted to assess the variation of the structural behaviour of the studied transmission lines associated with the location of the tornado relative to the tower of interest. The tornado-induced forces in both lines due to the three different normalized tornadoes are compared with corresponding values evaluated using the simplified load case method recently incorporated in the ASCE-74 (2020) guidelines, which was previously developed based on the research conducted at Western University.

• International Journal of Highway Engineering
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• v.9 no.4
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• pp.21-32
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• 2007

The linear traffic model(Vertical queueing model) that is adopted widely in traffic flow estimation assumes that all vehicles have the identical motion before joining a queue at the stop-line. Thus, a queue is supposed to form vertically not horizontally. Due to the simplicity of this model, the departure time of the leading vehicle is assumed to coincide with the start of effective green time. Thus, the delay estimates given by the Vertical queueing model is not always realistic. This paper explores a microscopic traffic model(a Kinematic Car-following model at Signalised intersections: a KCS traffic model) based on the one dimensional Kinematic equations in physics. A comparative evaluation in delay and sensitivity of delay difference between the KCS traffic model and the previously known Vertical queueing model is presented. The results show that the delay estimate in the Vertical queueing model is always greater than or equal to the KCS traffic model; however, the sensitivity of delay in the KCS traffic model is greater than the Vertical queueing model.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7137-7141
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• 2013

Several studies have shown that nemo-like kinase (NLK) plays a vital role in apoptosis of cancer cells. The present research concerned effects and mechanisms of Taxol on NLK knockdown human laryngeal cancerHep-2 cell lines in vitro. Using RNAi, methyl-thiazoltetrazolium (MTT) assays, real-time RT-PCR, Western blotting and flow cytometry analysis, growth and the cell cycle progression of NLK knockdown Hep-2 cells and expression of downstream molecules were observed. Cell growth was obviously suppressed in the Taxol treated group (P<0.001, 48 hours). Cell numbers of combined Taxol-based chemotherapy with lentivirus mediated RNAi treatment group (Lv-shNLK+Taxol goup) were significantly different from NLK-specific siRNA lentivirus infected group (Lv-shNLK group) (p<0.001). Flow cytometry analysis revealed that Lv-shNLK+Taxol caused the G0/G1-phase DNA content to decrease from 44.1 to 3.33% (p<0.001) and the S-phase DNA content to increase from 38.4 to 82.0% (p<0.001), in comparison with the Lv-shNLK+Taxol group. Immunoblot analysis showed that knockdown of NLK led to significant reduction in the levels of cyclin D1, PCNA and PARP, whereas cyclin B1 was elevated in. Cell growth was also obviously suppressed in the Hep-2 cell line, knockdown of NLK making them more sensitive to Taxol treatment. NLK is expected to become a target of new laryngeal cancer gene therapies.

• Journal of Energy Engineering
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• v.12 no.2
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• pp.93-100
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• 2003

An estimation methodology of the mass and energy (M/E) release due to the main steam line break (MSLB) has been developed with the RETRAN-3D code. In the case of equipment qualification (EQ), the over-estimated temperature would exceed the design limits of some cables or valves. In order to have a more flexible EQ profiles from the MSLB M/E release, the methodology with the best-estimated code was used. The major conditions affecting the MSLB M/E were found to be the initial SG level, heat transfer between primary and secondary sides, power level, operable protection system, main or auxiliary feedwater availability, and break conditions. The RETRAN-3D models were developed for the Kori unit 1 (KRN-1) which is typical two loop Westinghouse (WH) designed plant. Particularly, a detailed model of the steam generators was developed to estimate a more realistic two-phase heat transfer effect of the steam flow. After the modeling, the methodology has been developed through the sensitivity analyses. The M/E release data generated from the analyses have been used as the input to the inside containment pressure and temperature (P/T) analysis. According to the results at the point of view containment P/T, the Kori unit 1 can have more margin of 5∼15 ㎪ in pressure and 8∼15$^{\circ}C$ in temperature.

• Journal of Biosystems Engineering
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• v.36 no.2
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• pp.140-146
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• 2011

This study was performed to develop a rapid test kit for pathogenic Salmonella in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and biotin conjugated Salmonella antibodies were used as a tag and a receptor, respectively. Manually spotted Salmonella antibody and Neutravidin on nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Salmonella typhimurium in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Salmonella spiked in PBS. Also, the rapid test kit could detect $10^6$ cfu/mL of Salmonella in chicken meat extract.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.65 no.4
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• pp.255-261
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• 2016

Korean Power System are operating a load shedding system to prevent voltage instability phenomenon caused by severe line contingencies. In order to apply the load shedding scheme should be selected a location, amount, delay time. Current load shedding system is load shedding amount that has been calculated in the steady-state analysis to load shed the total amount in first level, load shedding amount calculated in advance, it is possible to perform an unnecessary load shedding. In this paper, set a multi-level load shedding control strategy step-by-step selection of load shedding amount for the prevention of excessive load shedding. In addition, through a voltage resilience analysis of the power system by applying motor load ratio and sensitivity parameter to selection the multi level load shedding ratio and delay time. For this reason, to take advantage of the limit data of interchange power, by utilizing interface power flow data to set a multi-level load shedding control strategy for the stabilization of the Korean Power System.

• Korean Journal of Agricultural Science
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• v.40 no.2
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• pp.139-146
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• 2013

This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect $10^5$ cfu/mL of Staphylococcus in chicken meat extract.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.53 no.2
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• pp.111-120
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• 2004

This paper presents an application of Unified Modeling Language(UML) software technique for developing transmission pricing evaluation package. Also, this paper describes a transmission pricing algorithm applicable to a large-scale power system. The usage-based transmission pricing mechanism is very complex since it requires power flow analysis, fault current analysis, sensitivity evaluation of a transmission line, penalty factors calculation, transmission asset databases, and cost allocation rules, etc. For the efficient and flexible development of the transmission pricing package, a UML. approach is applied, which is composed of a use-case diagram, interaction diagram, class diagram, and package diagram using Rational Rose Unified Process(RUP). The designed transmission pricing package can be efficiently modified and reused as the market environments evolves since it is designed by Object-Oriented Programming(OOP).

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.2227-2232
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• 2003

For a reasonable analysis of grade efficiency of cyclone separator, in-line measurements of particle size and particle concentration are needed. The purpose of this study is to define the correlation of measurement data and to measure grade efficiency and pressure drop. The authors adapted that the grade efficiency could be calculated directly from the counted particle numbers. The problem of collection efficiency calculation was different from counting rate of aerosol counters under the same conditions. It is important factor to use the rate of the particle counters to obtain reliable results. Therefore, the authors should use and calculate compensation factor considering sensitivity of aerosol counters, gas velocity and concentration affect higher 30% than originally measured grade efficiency. As the pressure drop and grade efficiency increase, as flow rate increase and then also pressure drop and grade efficiency increase.