• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7339-7344
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• 2013

To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.1
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• pp.49.2-49.2
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• 2011

To investigate whether radio galaxies have systematically different accretion disk compared to radio-quiet AGN, we obtained high quality optical spectra for a sample of 22 young radio galaxies, using the KAST Double Spectrograph at the Lick 3-m telescope. Young radio galaxies are particularly useful since the age of the radio phenomena is comparable to that of accretion disk. Based on the optical emission-line diagnostics of narrow line region, which is thought to be photoionized by the nuclear radiation, we constrain the states of the accretion disk. In addition to strong emission lines, i.e., [O I], [O II], [O III], and [Ne III], we use the [Ar III] line to break the degeneracy between the ionization parameter and the SED shape. We find that young radio galaxies show systematically different emission line ratios compared to radio-quiet Type II AGN, suggesting that young radio galaxies probably have the power-law SED without a strong big blue bump. We will present the main results of the emission-line diagnostics.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.04c
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• pp.111-114
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• 2008

The paper considers the relation between on-line monitoring and diagnostics on the one hand and high-voltage (HV) withstand and partial discharge (PD) on-site testing on the other. HV testing supplies the basic data (fingerprints) for diagnostics. In case of warnings by on-line diagnostic systems, off-line withstand and PD testing delivers the best possible information about defects and enables the classification of the risk. Because alternating voltage (AC) is the most important test voltage, the AC generation on site is considered. Frequency tuned resonant (ACRF) test systems are best adapted to on-site conditions. They can be simply combined with PD measuring equipment. The available ACRF test systems and their application to electric power equipment -from cable systems to power transformers - is described.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.13 no.2
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• pp.129-139
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• 2009

Sagittal spinal balance means standing postural balance at sagittal plane. Postural imbalance with displacement of the patient's center of gravity can cause chronic back pain and ambulatory difficulty. The sagittal spinal balance is determined based on the deviation of the C7 plumb line, originating at the middle of the C7 vertebral body, from the posterior superior endplate of S1. The line is called as sagittal vertical axis (SVA). In the traditional Korean medicine, the meridian sinews, which are the most superficial pathways of the meridian system, associated with movement, muscle balance and defense. They too are separate from the main meridians, though they intersect the main meridians. Some creative and pioneer researchers in Korea thought that the anatomy trains, which suggested by Myers is a concept familiar to the meridian sinews. A reciprocal relationship between the superficial back line and the superficial front line used to be compared to the rigging of a sailboat. Therefore, We suggest that spine may be compared to a mast of the sailboat and that the sagittal spinal balance can be maintained with systemic reciprocal interacts between the front line muscles and the back.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.04c
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• pp.103-105
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• 2008

In, this paper, we consider the relation between on-line monitoring and diagnostics on the one hand and high-voltage (HV) withstand and partial discharge (PO) on-site testing on the other. HV testing supplies the basic data (fingerprints) for diagnostics. In case of warnings by on-line diagnostic systems, off-line withstand and PO testing delivers the best possible information about defects and enables the classification of the risk. Because alternating voltage (AC) is the most important test voltage, the AC generation on site is considered. Frequency tuned resonant (ACRF) test systems are best adapted to on-site conditions. They can be simply combined with PO measuring equipment. The available ACRF test systems and their application to electric power equipment -from cable systems to power transformers - is described.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.2008-2009
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• 2007

The paper considers the relation between on-line monitoring and diagnostics on the one hand and high-voltage (HV) withstand and partial discharge (PD) on-site testing on the other. HV testing supplies the basic data (fingerprints) for diagnostics. In case of warnings by on-line diagnostic systems, off-line withstand and PD testing delivers the best possible information about defects and enables the classification of the risk. Because alternating voltage (AC) is the most important test voltage, the AC generation on site is considered. Frequency tuned resonant (ACRF) test systems are best adapted to on-site conditions. They can be simply combined with PD measuring equipment. The available ACRF test systems and their application to electric power equipment -from cable systems to power transformers - is introduced.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.2114-2115
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• 2008

We consider the relation between on-line monitoring and diagnostics on the one hand and high-voltage (HV) withstand and partial discharge (PD) on-site testing on the other. HV testing supplies the basic data (fingerprints) for diagnostics. In case of warnings by on-line diagnostic systems, off-line withstand and PD testing delivers the best possible information about defects and enables the classification of the risk. Frequency tuned resonant (ACRF) test systems are best adapted to on-site conditions. They can be simply combined with PD measuring equipment. The available ACRF test systems and their application to electric power equipment -from cable systems to power transformers is described.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.74.4-75
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• 2015

We investigate the narrow emission-line ratios of 64 radio-loud (log $L_{1.4GHz}$ > 40) AGNs available in the SDSS archive, in order to examine whether there is a systematic difference in the accretion disk condition of radio-loud AGNs compared to radio-quiet AGNs and compact young radio-loud AGNs. The fluxes of narrow-emission lines, [O II], [Ne III], [O III], [O I], [Ar III], are measured for diagnostics. Based on the [O I]/[O III] and [Ar III]/[O III] ratios with photoionization models, we constrain the states of the accretion disk. We will present the results of the emission-line diagnostics.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.185.1-185.1
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• 2016

Cutoff probe, diagnostics instrument for plasma density, have been received an extensive attention due to simple, robust and lowest assumption. Although the cutoff probe has a long history, physical model is limited in low density plasma. For that reason, we propose a novel transmission line model of cutoff probe for precise measurement of high density plasma. In addition simplified circuit model can be obtained from transmission line model. It can explain simply physics of cutoff probe in high density plasma.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.98-99
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• 2012

Deposition of thin films using magnetron sputtering plasmas is a well-developed, classical technology. However, detailed investigations using advanced diagnostics are insufficient in magnetron sputtering, in comparison with plasma-aided dry etching and plasma-enhanced chemical vapor deposition. In this talk, we will show examples of diagnostic works on magnetron sputtering employing metal targets. Diagnostic methods which have fine spatial resolutions are suitable for magnetron sputtering plasmas since they have significant spatial distributions. We are using two-dimensional laser-induced fluorescence spectroscopy, in which the plasma space is illuminated by a tunable laser beam with a planer shape. A charge-coupled device camera with a gated image intensifier is used for taking the picture of the image of laser-induced fluorescence formed on the planer laser beam. The picture of laser-induced fluorescence directly represents the two-dimensional distribution of the atom density probed by the tunable laser beam, when an intense laser with a relatively wide line-width is used. When a weak laser beam with a relatively narrow linewidth is used, the laser-induced fluorescence represents the density distribution of atoms which feel the laser wavelength to be resonant via the Doppler shift corresponding to their velocities. In this case, we can obtain the velocity distribution function of atoms by scanning the wavelength of the laser beam around the line center.