• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.226-230
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• 2007

In order to achieve high-level expression of interferon-${\alpha}1$ (IFN-${\alpha}1$) during fed-batch fermentation of recombinant E. coli, effects of oxygen supply and induction temperature on the expression of recombinant proteins were evaluated. Supplementation of oxygen and its transfer into cells is one of the most important parameters involved in the design and operation of mixing-sparging equipment for bioreactors. Generally, higher oxygen supply stimulates cell growth of aerobic microorganism and consequently the amount of products is increased. In this study, the optimum aeration strategy for the higher production of IFN-${\alpha}1$ during fed-batch fermentation of recombinant E. coli was surveyed. The growth of the cells was also monitored with four different concentrations of dissolved oxygen (DO; limiting, 20%, 35%, 50%) conditions. The DO was controlled by varying aeration rates of air and pure oxygen. Oxygen uptake rate (OUR) and specific oxygen uptake rate (SOUR) were evaluated and compared for the enhanced growth and induction of the cells and IFN-${\alpha}1$, respectively. We confirmed that increased DO by additional oxygen supply, up to 35%, can improve the production of IFN-${\alpha}1$ during the fermentation.

• Journal of Environmental Health Sciences
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• v.34 no.3
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• pp.255-260
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• 2008

This study was conducted to analyse the effects of the UFCA for treating polluted water in a reservoir. The UFCA mixes water by circulation of surface and bottom water layers. The circulation supplies oxygen to bottom of the reservoir, resulting in water quality improvement. With a UFCA in use, we surveyed the changes of temperature, pH, transparency, depth, conductivity, DO, COD, BOD, T-N, T-P and Chlorophyll-a for 7 months from Feb. to Aug. in 2004 in our experimental reservoir. There was little difference in the surface and bottom temperatures of the reservoir because of water mixing by the UFCA. However, pH was changed from 7 to 9. The transparency of water was about 80 cm through the all periods. Conductivity was $150\;{\mu}S/cm$ in early Feb., but increased to $270\;{\mu}S/cm$ in early March. Little change was seen in DO with depth, but it was maintained above 6 mg/l in June and July. BOD increased from 2.1 to 12.2 mg/l. The study reservoir did not undergo any eutrophication during the period of our experiment, but the comparison reservoir had an algae-bloom. The COD in the experimental reservoir increased from 5.4 to 14.5 mg/l. The COD concentration of the experimental reservoir was higher than comparison reservoir at the beginning of the study but in August this situation was reversed. SS concentration increased from 13.5 to 23.5 mg/l in Feb., but it fell from between 8.5 to 11.2 mg/l in July. T-N was increased from 1.3 to 4.9 mg/l. It increased up to 3 times in the rainy season as compared to other components. However the comparison reservoir increased up to 40 times higher than the experimental reservoir in the same period. T-P increased from 0.04 to 0.17 mg/l. The ratio of T-N to T-P increased from 20:1 to 40:1 which means that T-P was a growth limiting factor for algae and aquatic plants. Chlorophyll-a increased from 20 to 120 mg/l, and its concentration was correlated with T-P, such that Chlorophyl-a concentration increased with increased of T-P concentration. The concentrations of COD, T-N, T-P and other parameters were higher in the experimental reservoir than in the comparison reservoir but this situation was reversed in July, when the most severe eutophication occurred. The results show that overall the experimental reservoir was greatly remedied by UFCA. The UFCA accelerated the degradation of aquatic organic materials through effective supply of air with up-flow and circulation of water. We conclude that the UFCA can be very effective in aspect of the remediation of water quality incontaminated reservoirs and lakes.

• Korean Journal of Agricultural Science
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• v.6 no.1
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• pp.65-68
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• 1979

Water of river gets great concern about its quality, which might be polluted with pollutant from various industrial plants booming along the river sides in recent years. As a first step to prevent any applciable damage due to water pollution, survey on the water quality of Keum river at Sintanjin, Maepo, Taepyongri, Kongju, Puyeo and Kanggeng, six regions was conducted during september, 1977 through august, 1978 and the results were summarized as follow: 1. There were no significant regional and monthly difference in pH, $NO_2-N$, and $NO_3-N$ of water. 2. The BOD at Puyeo, Kanggeng was 3.9 to 5.6, 5.6 to 6.0 ppm, respectively, and those values reached the limiting level for tap water sources. 3. The BOD at Maepo, where Daejeon streams meet, was found 1.9, 3.0, and 2.1 times higher than those at Sintanjin region, during september, october and november, respectvely. 4. Content of DO was $7.6{\pm}3.8ppm$ in all surveyed regions and varied with water temperature and regional difference. 5. There were found regional difference and monthly difference in water hardness, suspended solid and $NH_3-N$.

• Journal of Bio-Environment Control
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• v.6 no.1
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• pp.27-33
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• 1997

This experiment was carried out to investigate the effects of limited nutrient supply during 21 days before and after pollination stage on the growth, fruit quality and nutrient uptake of muskmelon in rockwool culture. Muskmelon, cv. Earl's Favorite seeds sowed on rockwool cube and transplanted on rockwool slab($90\times15\times7.5cm$) when 2 to 3 true leaf appeared on Sep. 6, 1991. Three kinds of nutrient composition recommended by Shizuoka university, combinated with the composition of Otsuka house A and composition Shizuoka III. One half of calcium nitrate(Ca(NO$_3$)$_2$.4$H_2O$) for limiting nitrogen supply during 21 days was treated and then fertigated the nutrient composition recommended by Shizuoka university up to harvest time. Trickling nozzles(Netafim Co. Israel) were used for fertigation of nutrient solution and noncirculating system was employed. Temperature was maintained $18^{\circ}C$ in night but 23 to $25^{\circ}C$ for 10 days after pollination for softening the fruit. The drainage ratio of nutrient solution was adjusted 20 to 30 percent. Fertigated and drained amount, and the pH and EC of nutrient solution were recorded. The concentrations of mineral elements including N, P, K, Ca, and Mg were analyzed and compared among treatments. In both autumn and winter cultivation, the limitation of nutrient supply by adjustment of nutrient composition(NO$_3$-N : 8meㆍ$\ell^{-1}$) caused the nutrient deficiency in muskmelon plant due to the limited nutrient supply. After pollination nutrient limitation by the lowering the nitrate retarded the over thickening of upper leaves of muskmelon but plant height and fresh weight of fruit were higher in the plot of nonlimited nutrient supply. The phenomena were attributed to the differences of the amount of nutrient uptake due to the limited time of nutrient solution, duration of nutrient supply and concentration of nutrient solution. These results suggested that increasing nutrient supply in the pollination stage was favorable for better appearance of fruit and improving fruit quality. Further trials would be required for the incre-ment of sugar degree of muskmelon grown in rockwool.

• Korean Journal of Microbiology
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• v.34 no.4
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• pp.188-193
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• 1998

The authors examined the variations of environmental factors, the distributions of cyanobacteria, heterotrophic bacteria, and microorganisms that inhibit the growth of Anabaena cylindrica according to development and extinction of cyanobacterial bloom at a site in Daechung Dam reservoir. And certified the relationship between each other. Water temperature variated in a typical pattern. pH and concentrations of dissolved oxygen and chlorophylla was high in bloom period, and lowered with the decline of bloom. Phosphorus played as a growth-limiting factor at this study site. Total nitrogen concentration increased during blooming period, which indicated that nitrogen has been fixed by aquatic organisms such as cyanobacteria. Cyanobacteria distributed from June 17, and such cyanobacterial species as Anabaena spp., Aphanizomenon spp., Microcystis spp., Oscillatoria spp. and Phormidium spp. was detected during study period. Anabaena spp. distributed relatively highly distributed from July 23 to September 22, and disappeared completely at September 29. Heterotrophic bacterial and cyanobacterial populations varied inverse-proportionally. There was a relevancy between the variations of Anabaena spp., heterotrophic bacteria, and microorganisms that inhibit the growth of Anabaena cylindrica. Microorganisms that inhibit the growth of Anabaena cylindrica distributed from early growth phase of Anabaena spp. population to immediately after the extinction of Anabaena spp. With the population of Anabaena cylindrica growth-inhibiting microorganisms decreasing, increases of heterotrophic bacterial population followed it. Thease results indicate that microorganisms have a part in the extinction of cyanobacterial bloom, especially at its destroying period.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.10 no.3
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• pp.164-170
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• 2005

To investigate the aquatic environmental factors and processes controlling primary production in the Seomjin River estuary, chlorophyll a, nutrients, pH, SS, DO, temperature, salinity and primary productivity were measured in February, April, August and October, 2001. Primary productivity values ranged between 50.7 and 14,120.3 mg C $m^{-3} day^{-1}$ during the sampling period. In contrast to other estuaries, light condition did not seem to be the important limiting factor far primary production due to high water-column transparency during most of the time. The autumn bloom occurred in regions where salinity values ranged between 10 and 20 psu. This phenomenon appeared to develop every year and deserves further investigation. The behavior of nutrients, which is one of the major factors controlling the primary productivity, appeared to be governed by salinity regimes. The main source of nitrogenous nutrients seemed to be the freshwater runoff from the Seomjin River. However, that of phosphorus seemed to be from the industrial wastewater in Gwangyang area. The primary pro-duction of phytoplankton in the study area varied with space and time, showing a close correlation with water column transparency, and exhibited higher values compared to those of adjacent coastal regions in Gwangyang Bay.

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.13-22
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• 2006

Gonadosomatic index (GSI), oogenesis and reproductive cycle of Glossaulax didyma were investigated by the cytological histological obserbations and morphometric data. Samples were collected monthly from the intertidal zone of Biin Bay, Seocheon, Korea, for one year. Monthly variations in the GSI showed similar patterns with gonadal development. In the early vitellogenic oocyte, the Golgi complex and mitochondria were invovled in the formation of lipid droplet and yolk precursor. In the late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of profeid yolk granules in the cytoplasm. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting membrane. The spawning season was from early June to late August, and the main spawning occurred between July and August when the seawater temperature was above $19^{\circ}C$. The female reproductive cycle can be classified into five successive stages: early active stage (December to February), late active stage (February to March), ripe stage (April to July), spawning stage (June to August), recovery stage (August to November). Fully mature oocytes were approximately $250-270{\mu}m$ in diameter.

• Genes and Genomics
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• v.40 no.11
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• pp.1181-1197
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• 2018

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at $4^{\circ}C$ for 2 h) and LT24 (cold treatment at $4^{\circ}C$ for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.178-184
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• 2019

The objective of this study was to evaluate the dry matter yield of (DMY) of Italian ryegrass (IRG) forage crop based on two seeding date (September 25 and October 2) in 2017 and 2018, and evaluation of the potential reason of DMY reduction in IRG cultivars grown at Chuncheon, South Korea. Three cultivars of IRG including Kowinearly, Kowinmaster and Hwasan 104 were used in this experiment. The experimental field was designed as a split-split-plot arrangement. The cultivars were harvested on April 26 before the heading stage of both years. In this study, no significant effect (p>0.05) of seeding date has been found on the DMY, whereas the cultivars and year showed a significant effect on the DMY (p<0.05). Except September 25 of 2017, Hwasan 104 indicated significantly lower (p<0.05) DMY than Kowinearly and Kowinmaster in all seeding periods. Though no significant difference (p>0.05) was observed for the DMY between the two seeding dates in 2018, but the DMY was significantly lower (p<0.05) than that of 2017. Lower crude protein (CP) content of Kowinearly was observed on the seeding date of October 2 of 2017, and also in both seeding dates on 2018 (p<0.05). No significant difference (p>0.05) was observed in acid detergent fiber (ADF) between the cultivars on both seeding dates and years. Similarly, no significant difference (p>0.05) was found in neutral detergent fiber (NDF) between the cultivars in 2017. However, Kowinearly showed higher NDF content as compared to Kowinmaster and Hwasan 104 on both seeding dates and years. The seeding date has no significant difference (p>0.05). Thus, considering the extent of DMY in both years, the mean minimum temperature during winter season could be the limiting factor for the DMY of IRG cultivars. However, based on this study, the seeding dates of September 25 and October 2 have no potential effect on the DMY. Thus, on the basis of the current DMY and forge quality parameters, the cultivation Kowinmaster is recommended in Chuncheon.

• Applied Chemistry for Engineering
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• v.33 no.2
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• pp.151-158
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• 2022

In this paper, parameter characteristics such as pH effect, isotherm, kinetic and thermodynamic parameters and competitive adsorption of dyes including malachite green (MG), direct red 81 (DR 81) and thioflavin S (TS), which have different functional groups, being adsorbed onto activated carbon were investigated. Langmuir, Freundlich and Temkin isotherm models were employed to find the adsorption mechanism. Effectiveness of adsorption treatment of three dyes by activated carbon were confirmed by the Langmuir dimensionless separation factor. The mechanism was found to be a physical adsorption which can be verified through the adsorption heat calculated by Temkin equation. The adsorption kinetics followed the pseudo second order and the rate limiting step was intra-particle diffusion. The positive enthalpy and entropy changes showed an endothermic reaction and increased disorder via adsorption at the S-L interface, respectively. For each dye molecule, negative Gibbs free energy increased with the temperature, which means that the process is spontaneous. In the binary component system, it was found that the same functional groups of the dye could interfere with the mutual adsorption, and different functional groups did not significantly affect the adsorption. In the ternary component system, the adsorption for MG lowered a bit, likely to be disturbed by the other dyes meanwhile DR 81 and TS were to be positively affected by the presence of MG, thus resulting in much higher adsorption.