• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.475-481
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• 1996

This study was carried out to acquire the basic information of root growth under different pot size, imposing different space limitation on rhizosphere. Different size of pots that had same surface area but different depth, 5cm(Iength)$\times$5cm(width)$\times$30, 15, 5cm(depth), were used during the seedling stage of tobacco plant. Space limitation on rhizosphere affected not only the aerial growth, stem height, leaf area and shoot dry weight, but also root growth and root architecture. Aerial growth was highly related to growth of underground part, so space limitation on rhizosphere decreased aerial growth. Limitation on pot volume by reducing pot depth induced new rooting on crown. Root number and relative multiplication rate were higher in small pot that had 5cm depth than large pot, but total root length and mean extension rate showed reverse patterns. Root numbers of 1st order and 2nd order were increased as pot depth was increased, but the root number of 3rd order was increased in small pot. Root system of seedling grown in large pot distributed more horizontally than that in small pot at 20 days after temporary planting (DAT), but the root architecture of seedling was reversed at 25 DAT.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.970-972
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• 2003

Due to rapid increase in the demand of Natural Rubber (NR) few years ago, NR price sore very higher. The rubber plantation in Thailand expanded very fast to non traditional areas with the result Thai become the biggest NR exporting country in the world. However, the average yield is still lower compared to experimental yield of RRIT (Rubber Research Institute of Thailand) or just 60 % (RRIT, 1998). This is due to many of new rubber planting areas, which are not suitable. The Thai Ministry of Agriculture and Cooperatives thus has set 'The complete cycle development strategies for natural rubber' in the medium-term measures by reducing the rubber planting areas by 300,000 rai (1 rai = 0.16ha) through replanting with oil palm. The aim of this study is to find out land having lowest potential for rubber production (R3) but highest for oil palm production (P1). Find areas which are unsuitable for rubber and can be replaced by oil palm in order to get a better agricultural production. The study was applied upon Krabi province, Thailand. Crops requirement, degree of limitation to crops growth, climatic data, crops yield, soil map, topographic map etc., were used to evaluate land potential for both rubber and oil palm production according to FAO framework (Sys, 1992). An Agro-ecological suitability map for rubber and oil palm were produced. This was done by mean of GIS. The database was generated and guide map for the decision makers in view of suitable crop substitution was prepared.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.3
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• pp.196-207
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• 2016

In monsoon climates, persistent shade is a troublesome weather condition with an impact on the growth and yield of corn (Zea mays L.). We imposed 7, 14, 21, and 28 days of consecutive shade (CS) on Gwangpyeongok and P3394 corn hybrids at the beginning of the kernel filling stages. Shade had little impact on leaf area and dry matter accumulation in the stem and leaves. However, dry matter accumulation in the ear was severely reduced by approximately 28% and 53% after 14 and 28 days of CS, respectively. For the components of grain yield, 7 and 14 days of shade did irreparable damage to the number of filled kernels, the kernel number per ear row, and the percent of filled kernels, but did little damage or reversible damage after removal of the shade to the 100-grain weight and the row number per ear. Shade significantly reduced the relative growth rate (RGR) due to a decrease in the net assimilation rate (NAR). These results suggest that source activity limitation by shade during the kernel filling stages leads to the inhibition of sink activity and size. The yield of biomass, ear, and grain logistically declined as the length of CS increased. Probit analysis revealed that the number of days of CS needed to cause 25% and 50% reductions in grain yield were 3.7 and 23.1, respectively. These results suggest that the plant yield loss induced by shade at the beginning of the kernel filling stages is mainly achieved within the first 7 days of consecutive shade.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.22-33
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• 1991

The studies aimed to distinguish between initial (ionic or osmotic) effects of salinisation on growth and the longer-term consequences of excessive salt accumulation. Tall and dwarf varieties of rice were used to provide different growth rates. There was no significant effect upon the day-to-day pattern of growth, upon the ultimate length of leaves that were developing at the time of, or shortly after, salinisation with 50 mM NaCl. Leaves that developed after prolonged exposure of the plants to salinity were shorter. Addition of NaCl, KCl or mannitol to the root medium brought about a cessation of leaf elongation within one minute. Growth at a reduced rate restarted abruptly after a lag period that depended upon the external concentration. Elongation rate recovered to its original value within 24 hours after exposure to 50 mM NaCl, though not at higher concentrations. Addition of NaCl at concentrations up to 100 mM elicited no short-term effect upon photsynthetic gas exchange. No change in turgor pressure was detectable in the growing zone with the resolution of the miniature pressure probe used (about 70 kPa). It is concluded that the initial growth reduction in rice caused by salinisation is due to a limitation of water supply. A clear distinction is made between the initial effects of salt which are recoverable, and the long-term effects which result from the accumulation of salt within expanded leaves.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.111-116
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• 2015

Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.1
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• pp.68-73
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• 2002

High temperature during sprout emergence period of potato (Solanum tuberosum L.) is a major limitation to the yield of fall-grown potato in Korea. To explore the possibility of improving the yield of fall-grown potato through recycled paper mulching, the changes of soil environment and the growth and yield of potato cv. Daeji as affected by three mulching treatments of non-mulched control, recycled paper mulching (RPM), and black polyethylene film-mulching (BPFM) were examined over two fall seasons at Suwon, Korea. The mulching materials were a recycled mulch paper with 110 g/$m^2$ and a thickness of 0.1 mm, which was manufactured from old corrugated containers for this experiment and a commercial black polyethylene film with a thickness of 0.01mm. On an average throughout the growing period, the soil temperature with RPM at the 5-cm depth was lower by 0.4-1.1$^{\circ}C$ than that of the control. The maximum temperature during daytime in RPM was lowered by approximately 1 to 5$^{\circ}C$ according to the weather condition during emergence period, the difference being great on a fine day. The soil temperature with BPFM was much higher than the control. The effect of the mulching treatments diminished as the canopy became developed. The mulching treatments were more advantageous than the control in the conservation of soil water. Moreover, RPM and BPFM efficiently suppressed the occurrence of weeds until the potato harvest. RPM improved the emergence significantly due to lower soil temperature, whereas BPFM showed much poorer emergence than the control. Growth after emergence and yield were significantly higher in RPM than in the control and BPFM. It was concluded that the significantly improved yield in RPM compared to that in the control was mainly due to the improvement of the sprout emergence and tuber growth accompanied by lower soil temperature and better conservation of soil water.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.632-639
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• 1997

The experiment which imposed the water stress to tobacco(Nicotiana tabacum L.) plant was carried at the late of maximum growth period. In order to know the influence of drought stress on the growth and developmemt of tobacco leaves of different position and to elucidate the physiological response of plant to various soil water content, stomatal conductance, and leaf water potential were measured. The drought stress at the maximum growth period negatively affected to the overall growth characteristics of shoot. The response of the growth was small at the middle and the lower leaves, and great at the upper leaves. The relative water content of upper, middle, and lower leaves at the fifth day after treatment were 74, 64, and 59%, respectively, as soil water content was reduced by 4.3%. This suggested that the wilting point of tobacco leaf was about 75%. The leaf water potential was -0.58 MPa in control and dropped to -1.20 MPa at the fifth day after treatment. This indicated that wilting of leaf may occur at the condition in which the difference of water potential between treatment and control, well watered, was greater than about 20%. Stomatal conductance at the fifth day after treatment dropped from 12 mol /$\textrm{m}^2 sec^{-1}$ to 0.8 mol /$\textrm{m}^2 sec^{-1}$ in the middle and the upper leaves. Stomatal conductance of lower leaves already matured were not affected highly by drought stress at the maximum growth period, but maturing leaves, middle and upper leaves, were highly affected by limitation of soil water.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.3
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• pp.47-54
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• 1978

Weed control is an essential part in rice culture and has been practiced by hand. But the shortage of man power in rural area in recent years has brought about the use of herbicides. The effective use of herbicides in rice has contributed toward improving labor situation and maintaining rice production. The use of herbicides will extend more in the future. The only limitation of present uses of monoherbicides might be the predominant growth of a certain weeds in paddy field, and subsequent effect on ecology system. It is, therefore, wanted to develop a herbicide which will be effective on several kinds of weeds. In the weed control system, ecological and integrated weed control would be necessary in the future. To obtain the most effective weed control, studies on physiological ecotype of weeds and machanism of herbicides and their interactions will be necessary.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.640-646
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• 1997

To determine variations in leaflet length (LL), leaflet width (LW), leaflet size (LS), and leaflet shape index (LSI), and their association with eight agronomic traits, characterization data of 884 soybean accessions which were grown in the autumn of 1992 in Taiwan were analyzed. LL ranged from 4.3 to 14.7 cm, and LW ranged from 2.8 to 9.7 cm. Also, LS (LL $\times$ LW) ranged from 12.1 to 124.6 $\textrm{cm}^2$. The absolute variation of LL, LW, and LS was not large because of limitation in vegetative growth by short day length. None was classified as a large leaflet based on the International Board for Plant Genetic Resources (IBPGR) descriptors. LSI (LL /LW) ranged from 1.21 to 3.06, and three accessions were classified as narrow leaflet. There were differences in ranges and means of LL, LW, LS, and LSI between and within temperate and tropical accessions. LL, LW, LS, and LSI had highly significant positive correlations with seven agronomic traits and highly significant negative correlation with 100-seed weight except LW for all accessions. There was variation in the closeness of association among leaflet traits, and between and within temperate and tropical accessions. Generally, LL, LW, and LS were more closely associated with days to flowering, plant height at $R_1$ and $R_8$, number of pods per plant; LSI was more closely associated with 100-seed weight than other traits.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.