• 환경생물
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• 제26권1호
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• pp.15-21
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• 2008

Ionizing radiation causes many alterations in photosynthetic machineries. However, there is no information about effects of ionizing radiation on the development of photosynthetic machineries in plants. We investigated the greening of etiolated mung bean seedlings after gamma-irradiation of 50 to 300 Gy. The irradiation inhibited seedling growth with great dependence on the radiation dose. In particular, growth of stems was more affected than that of hypocotyls. Irradiated leaves showed inhibition in growth, aberration in morphology, and yellowing in color depending on the radiation dose. Contents of photosynthetic pigments such as chlorophylls and carotenoids were significantly decreased in the irradiated leaves. The apparent electron transport rate for photosynthesis, ETR, was similarly changed depending on the radiation dose. However, the maximal photochemical efficiency of Photosystem II (PSII), Fv/Fm, was little affected by the irradiation. Moreover, the 50-Gy seedlings maintained the control level of light saturating for photosynthesis and showed slightly higher Fv/Fm values in spite of significant decreases in the photosynthetic pigment content and ETR. These results suggest that the inhibition of the overall photosynthetic capacity couldn’t be causally relatqaed with the repression in the initial development of irradiated seedlings and that the overall photosynthetic machineries can develop and work to some extent as a concerted system for photosynthesis even after exposure to acute doses of ionizing radiation.

• Journal of Photoscience
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• 제7권2호
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• pp.39-44
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• 2000

To examine the chilling tolerance lipids, we compared the chilling susceptibility of photosystem II of wild type tobacco plants with that of transgenic tobacco plants, in which the sensitivity to chilling had been enhanced by genetic modification of fatty acid unsaturation of chloroplast membrane lipids. The transgenic tobacco plants were found to contain reduced levels of unsaturated membrane fatty acids by being tansformed with cDNA for glycerol-3-phosphate acyltransferase from squash. For the purpose of studying on the functional integrity of photosystem II during low-temperature photoinhibition, the photochemical efficiency was measured as the ration of the maximun fluorescence of chlorophyll (Fv/Fm) of photosystem II. In parallel with an investigation on the transgenic plants, susceptibility of chilling-resistant species, such as spinah and pea, and of chilling-sensitive ones, such as squash and sweet potato, to low-temperature photoinhibition was also compared in terms of room temperature-induced chlorophyll fluorescence from photosystem II. When leaf disks from the two genotypes of tobacco plants were exposed to light at 5$^{\circ}C$, the transgenic plants showed more rapid decline in photochemical activity of photosysytme II than wild-type plants. When they were pretreated with lincomycin, an inhibitor of chloroplast-encoded protein synthesis, the extent of photoinhibition was even more accelerated. More impottantly, they showed a comparable extent of photoinhibition in the presence of lincomycin, making a clear contrast to the discrepancy observed in the discrepancy observed in the absence of lincomycin. Restoration of Fv/Fm during recovery from low-temperature photoinhibition occurred more slowly in the transgenic tobacco plants than the wild-type. These findings are discussed in relation to fatty acid unsaturation of membrane phosphatidylglycerol. It appears that the ability of plants to rapidly regenerate the active photosystem II complex from might explain, in part, why chilling-resistant plants can toleratlow-temperature photoinhibition.

• 한국환경과학회지
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• 제11권10호
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• pp.1023-1030
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• 2002

The effects on photosynthesis of NaCl(0, 0.2, 0.4, 0.6, 0.8 or 1.0 M) were examined in etiolated barley seedlings. Chlorophyll(Chl) a, Chl b and carotenoid contents, Chl a fluorescence and quenching coefficients of Chl fluorescence have been determined in the primary leaves of etiolated barley seedlings cultivated under low light(60 $\mu$㏖ $m^{-2}\;s^{-1}$). Chl a, b, and carotenoid contents were decreased remarkably in comparison with the control at 0.4 M NaCl. However, the value of Fo and Fv were decreased at 0.6 M NaCl and the ratio of Fv/Fm were deceased at 1.0 M NaCl. Chlorophyll synthesis was seriously inhibited from 0.4 M NaCl, and the photosynthetic electron transport system was inhibited from 0.6 M NaCl. Quantum of photosystem II reaction center was inhibited at 1.0 M NaCl. The effects of NaCl on the Chl content were raised in a 6 hrs, but the effects of NaCl on the value of Fo, Fv and Fv/Fm were raised in 30 hrs. The value of qP was decreased in comparison with the control at all concentrations, but there was a small change in the value qE. These results provide evidence that NaCl inhibited effects of various concentration of NaCl were inhibited quinone redox, however, proton gradient between thylakoid membranes was little damaged.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.61-61
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• 2019

본 연구에서는 실내 광조건에서 자생 장미과 상록 활엽 목본 2종의 생육반응을 조사하였다. 식물재료는 다정큼나무[Rhaphiolepis indica var. umbellata (Thunb.) Ohashi]와 비파나무[Eriobotrya japonica (Thunb.) Lindl.]의 3년생 실생묘를 사용하였다. 실내 광량은 10, 50, 100 및 200 PPFD (${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 설정되었으며, 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$), 습도($55{\pm}3%$) 및 관수(1회/3일) 주기는 일정하게 유지하였다. 실내에서 8주간 재배한 다음 유리온실에서 동일 기간동안 재배된 대조구와 생육 및 광합성 능력 등을 비교하였다. 연구 결과, 다정큼나무의 생육은 200 PPFD에서 대조구와 유사한 수준이었으며, 줄기직경은 광량과 정비례하는 경향이었다. 엽수는 대조구에 비해 고 광량(100, 200 PPFD) 조건에서 유의적으로 높은 결과를 보였다. 처리 별 최대 양자수율(Fv/Fm) 및 광계II 성능지수(Pi_Abs)는 대조구와 유사한 수준이었으며, 광량에 따른 유의적인 차이가 없었다. 비파나무는 재배기간 동안 광량에 관계없이 생육변화가 크지 않았으나, 엽록소함량은 200 PPFD에서 가장 많았다. 한편 10 PPFD에서는 모든 개체의 잎 고사에 따른 관상가치가 하락하였다. Fv/Fm은 200 PPFD에서 대조구와 유사한 수준이었으며, Pi_Abs는 저광량(10, 50 PPFD) 조건에서 유의적으로 높은 수치였다.

• 한국정보통신학회논문지
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• 제18권9호
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• pp.2280-2288
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• 2014

기존의 고출력 LED의 디밍(Dimming) 제어 방법에는 펄스폭변조, 주파수변조, 아날로그 제어 방법이 있으며, 아날로그 디밍은 순방향 고출력 LED 전류를 조정하는 방법으로 0%~100%의 전 범위에서 선형 밝기 조절이 가능하나 순방향 전류를 변화시킴으로써 파장의 변화가 존재하고, 펄스폭변조 디밍 제어는 고출력 LED에 흐르는 정격 전류의 듀티를 가변하여 조도를 제어하는 방식으로 저 레벨(0%~10%)에서 선형 밝기 조정이 어렵다. 그리고 주파수변조 디밍 또한 저 레벨의 디밍에서 빛이 깜박거리는 문제를 내포하고 있다. 본 논문은 이러한 디밍 방식의 단점을 보완하고자 저 레벨 디밍 제어 시 아날로그 방식을 적용하고, 10%~100%의 디밍 제어 시에 펄스폭변조 방식을 적용하는 효율적인 디밍 제어 회로의 설계 및 구현을 제안한다. 전 범위에 걸쳐 선형 밝기 조절이 가능하고, 다양한 디밍 레벨에서 일정한 파장의 출력이 가능한 디밍 제어 회로를 구현하였으며, 실험 결과는 제안한 방법의 우수성을 보여주고 있다.

• Fisheries and Aquatic Sciences
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• 제19권10호
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• pp.40.1-40.14
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• 2016

Background: Due to the paucity of oceanic resources utilized in the preparation of diets for cultured fish, commercial feed producers have been trying to replace fishmeal (FM) using alternative protein sources such as vegetable protein meals (VMs). One of the main drawbacks of using VMs in fish feed is related to the presence of a variety of anti-nutritional factors, which could trigger an inflammation process in the distal intestine. This reduces the capacity of the enterocytes to absorb nutrients leading to reduced fish growth performances. Methods: We evaluated the mitigating effects of butyrate and taurine used as feed additives on the morphological abnormalities caused by a soybean meal (SBM)-based diet in the distal intestine of sea bass (Dicentrarchus labrax). We used three experimental diets, containing the same low percentage of FM and high percentage of SBM; two diets were supplemented with either 0.2% sodium butyrate or taurine. Histological changes in the intestine of fish were determined by light and transmission electron microscopy. Infiltration of $CD45^+$ leucocytes in the lamina propria and in the submucosa was assessed by immunohistochemistry. We also quantified by One-Step Taqman$^{(R)}$ real-time RT-PCR the messenger RNA (mRNA) abundance of a panel of genes involved in the intestinal mucosa inflammatory response such as $TNF{\alpha}$ (tumor necrosis factor alpha) and interleukins: IL-8, IL-$1{\beta}$, IL-10, and IL-6. Results: Fish that received for 2 months the diet with 30% soy protein (16.7% SBM and 12.8% full-fat soy) developed an inflammation in the distal intestine, as confirmed by histological and immunohistochemistry data. The expression of target genes in the intestine was deeply influenced by the type of fish diet. Fish fed with taurine-supplemented diet displayed the lowest number of mRNA copies of IL-$1{\beta}$, IL-8, and IL-10 genes in comparison to fish fed with control or butyrate-supplemented diets. Dietary butyrate caused an upregulation of the $TNF{\alpha}$ gene transcription. Among the quantified interleukins, IL-6 was the only one to be not influenced by the diet. Conclusions: Histological and gene expression data suggest that butyrate and taurine could have a role in normalizing the intestinal abnormalities caused by the SBM, but the underling mechanisms of action seem different.

• 한국시뮬레이션학회논문지
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• 제28권2호
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• pp.139-147
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• 2019

적외선 에너지를 추적하는 휴대용대공유도탄(MANPADS: Man Portable Air Defense System)은 개인이 휴대할 수 있을 정도로 가벼우며 다양한 항공기에 대응할 수 있어, 전 세계적으로 널리 배치되어 항공기의 큰 위협이 되고 있다. 이러한 유도탄을 기만하여 아군 항공기와 전투원의 생명을 보호하는 적외선대응책(IRCM: Infrared Countermeasure)으로 섬광탄이 개발되었다. 그러나 기존 적외선대응책의 문제점을 보완할 수 있는 지향성적외선방해장비(DIRCM: Directional Infrared Countermeasure)가 최근 일부 선진국을 중심으로 개발되고 있으며, 그 필요성이 점차 증가하고 있다. 본 논문에서는 다양한 MANPADS 중 AM 변조 방식의 1세대 적외선탐색기, FM 변조 방식의 2세대 적외선탐색기, 펄스변조 방식의 3세대 적외선탐색기와 DIRCM에서 레이저빔이 조사될 때 탐색기에서 발생하는 산란광 현상을 모델링하였다. 이를 이용하여 MANPADS와 DIRCM을 장착한 항공기의 다양한 교전환경에서 기만 시뮬레이션을 수행하였고, 유도탄과 항공기의 최소거리인 Miss Distance를 분석하였다. 시뮬레이션 결과, 하나의 기만코드로 변조된 레이저빔을 조사하는 DIRCM이 교전거리 1km와 2km에서 1, 2, 3세대 MANPADS를 모두 기만하는 것을 시뮬레이션을 통하여 확인하였다. 특히 교전거리가 1km에서 2km로 늘어날 때 DIRCM을 장착한 항공기의 생존율도 증가하였고, 교전거리 2km에서는 생존율이 최소 99% 이상으로 분석되었다.

• 한국농림기상학회지
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• 제14권2호
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• pp.79-89
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• 2012

본 연구는 광환경 변화에 따른 병풍쌈의 엽록소 함량, 광합성 특성, 엽록소형광반응 그리고 생장 특성을 조사하기 위해 실시하였으며, 실험을 위해 병풍쌈을 무처리구(full sunlight)와 3개의 서로 다른 피음조건(88~93%, 65~75% and 45%~55% of full sunlight)에서 생육시켰다. 병풍쌈은 피음수준이 증가할수록 총 엽록소 함량, 광화학반응효율, T/R율, SLA, LAR, LWR은 증가하고 암호흡은 감소하여, 낮은 광조건에서 빛의 흡수량과 광합성에 대한 이용효율을 높이는 반응을 나타냈다. 5월의 중피음처리구에서 가장 높은 최대광합성속도와 순양자수율을 나타냈으며, 피음처리구보다 전광처리구에서 가장 낮은 최대광합성속도, 광화학반응효율, 엽록소 함량을 나타냈다. 위의 결과에 따라 병풍쌈은 음지식물로 강한 광에 대한 민감성이 매우 높고, 광을 제한하여도 강피음 상태를 지속하게 되면 광합성 능력이 크게 감소될 수 있으며, 건전한 생육을 위해서는 노천광의 65~75%를 차단시킨 광환경 조건이 적합하다고 생각된다.

• BMB Reports
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• 제38권4호
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• pp.481-485
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• 2005

The response of Spirulina (Arthrospira) platensis to high salt stress was investigated by incubating the cells in light of moderate intensity in the presence of 0.8 M NaCl. NaCl caused a decrease in photosystem II (PSII) mediated oxygen evolution activity and increase in photosystem I (PSI) activity and the amount of P700. Similarly maximal efficiency of PSII (Fv/Fm) and variable fluorescence (Fv/Fo) were also declined in salt-stressed cells. Western blot analysis reveal that the inhibition in PSII activity is due to a 40% loss of a thylakoid membrane protein, known as D1, which is located in PSII reaction center. NaCl treatment of cells also resulted in the alterations of other thylakoid membrane proteins: most prominently, a dramatic diminishment of the 47-kDa chlorophyll protein (CP) and 94-kDa protein, and accumulation of a 17-kDa protein band were observed in SDS-PAGE. The changes in 47-kDa and 94-kDa proteins lead to the decreased energy transfer from light harvesting antenna to PSII, which was accompanied by alterations in the chlorophyll fluorescence emission spectra of whole cells and isolated thylakoids. Therefore we conclude that salt stress has various effects on photosynthetic electron transport activities due to the marked alterations in the composition of thylakoid membrane proteins.

• ALGAE
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• 제20권2호
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• pp.157-166
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• 2005

Uptake kinetics of Cd and Zn by leaves and rhizome of the seagrass Zostera marina were examined in controlled laboratory radiotracer experiments. Subsequently, acute toxicity of Cd, Cu and TBT on photosynthetic quantum yield (ΔF/Fm’ of Z. marina were determined, and the differential sensitivities of rapid light curve (RLC) to those harmful substances were also compared. All measurements on photosynthetic activity were determined by chlorophyll a fluorescence method using pulse amplitude modulation (PAM). Metal uptake by Z. marina was saturated with increasing exposure time in leaves and rhizomes. Uptake of Zn by Z. marina was faster than that of Cd. Metal uptake rates in Z. marina decreased with the increase of dissolved metal concentrations and also with the increase of biomass. The adverse effect of TBT on effective quantum yield was stronger than other pollutants. Average acute toxicity on the RLC of the seagrass exposed to TBT and two heavy metals (Cd and Cu) was going to decrease as follows: TBT > Cd > Cu. Our preliminary results in this study suggested that Z. marina potentially can be used as a biomonitor of harmful substances contamination in coastal waters.