• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.21-29
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• 2020

Morphology of a strain of unspecified Skeletonema species established from Korean coast was examined by light, fluorescence and scanning electron microscopy, and SSU(small subunit) and LSU(large subunit) rDNA of the strain were also sequenced. The specimen was characterized by solitary or short chains, and each cell contained 1-2 chloroplasts. The valve face was slightly convex, and the terminal fultoportula processes (TFPPs) were open and showed narrow distal ends that could be truncated or spiny with claw-like protrusions. The basal part of the TFPPs was tubular and oblique to the cell axis. The intercalary fultoportula processes (IFPPs) were also narrow, completely open, and joined in a 1 : 1 junction. Occasionally, one IFPP was connected with two opposing IFPPs. The morphological features of the specimen were identical to those of Skeletonema pseudocostatum. Molecular phylogeny based on SSU rDNA revealed that the Korean strain is nested within a clade comprising S. pseudocostatum and S. tropicum. However, based on D1-D2 LSU rDNA sequences, a clade including S. pseudocostatum and a Korean strain was separated from the S. tropicum clade. This indicates that the Korean strain can be identified as S. pseudocostatum. This species represents the first record from Korean coastal waters.

• Journal of Veterinary Clinics
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• v.32 no.3
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• pp.239-242
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• 2015

Trypanorhych plerocercoids of Nybelinia surmenicola are described in the common squid, Todarodes pacificus, through the use of light and scanning electron microscopy. Upon dissection, out of 200 common squid from the Korean coastal water, 54 (27%) were found infected with N. surmenicola plerocercoids. Mean N. surmenicola abundance was 2.28 per squid. Body length and width ranged from $6.1-11.1(8.05){\times}2.0-2.2(2.1)mm$. Bothria are broad, bean-shaped, measured $2.4-2.8{\times}0.62-0.69(2.65{\times}0.64)mm$. The short and long tentacular armatures which are spirally arranged are homeoacanthous and homeomorphous uncinate hooks and consist of compact rose-thorn-shaped tentacular hooks. The tentacle sheaths are coiled in a spiral near the bulbs. Four large bothria are separated by filiform microtriches along the bothridal margins. This is the first description of the plerocercoids of N. surmenicola in the common of squid from the Korean coastal waters.

• Toxicological Research
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• v.34 no.3
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• Journal of Preventive Medicine and Public Health
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• v.29 no.1 s.52
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• pp.43-50
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• 1996

We experienced a case of nephropathy in chronic lead poisoning. The patient was 43-year-old male who has been working in secondary lead smelting plant for 14 years. On admission, blood pressure was 160/90 mmHg and the others were non-specific. In past history, he received chelating agent administration for lead poisoning irregularly and medicated for gout, and the blood lead concentration was $180.0{\mu}g/dl$ on 2 months before admission. Smoking habit has been 1 pack per day for 15 years and drinking habit has been 1 bottle of Soju per day but less flow. In liver function test, AST/ALT were 27/28 IU/l and $\gamma-GT$ was 456 IU/l. In blood test, Hb : 11.5 g/dl, Hct : 34.0% and basophilic stipplings were found in peripheral blood smear. Chest PA was normal and abdominal ultrasonographic finding was non-specific except fatty liver. In the test of lead exposure indices, $PbB:83.0{\mu}g/dl,\;PbU:28.3{\mu}g/l$, and blood ZPP was $300.0{\mu}g/dl$. And in renal function test, BUN : 31.4 mg/dl, blood creatinine : 2.7mg/dl, blood uric acid. 9.1 mg/dl, urinary albumin : 100.0 mg/g creatinine, urinary $\alpha_1-microglobulin$ : 120.5 mg/g creatinine, urinary $\beta_2-microglobulin$ : $183.8{\mu}g/g$ creatinine, and 24 hours urinary creatinine clearance was 31.9 ml/min. The ultrasonoguided renal biopsy showed the global sclerosis of glomerulus, moderate atrophy and loss of tubule, and interstitial fibrosis in light microscopy. There were diffuse losses of brush border of proximal tubule in electronmicroscopy.

• Childhood Kidney Diseases
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• v.1 no.2
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• pp.189-194
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• 1997

Oligomeganephronia is a rare congenital form of bilateral renal hypoplasia histologically characterized by reduction in number and hypertrophy of nephrons. Clinically, this condition is presented in early infancy with vomiting, polyuria, polydipsia and dehydration. The problems are readily corrected, but slowly progressive renal failure follows accompanied by failure to thrive, short stature, and renal osteodystrophy. We experienced three cases of oligomeganephronia. Case 1. : A 3 2/12 years old female child was incidentally diagnosed as renal failure at age of 2 months when she was hospitalized due to pneumonia. She had open renal biopsy and was diagnosed as bilateral dysplastic kidney. On OPD follow-up, she progressed to end-stage renal failure (BUN/Cr 114/4.6 mg/dl) and had renal transplantation. The specimen was shrunk remarkably and light microscopy showed oligomeganephronia. Case 2. : A 14 8/12 years old female child with proteinuria was detected in an annual urine screening program for school children, she was diagnosed as renal failure (BUN/Cr 33.9/4.1 mg/dl), and had $5{\times}4{\times}3\;cm$ sized mass on abdominal CT scan. She had renal biopsy, and the specimen showed oligomeganephronia. She had hemodialysis for six months, and renal transplantation along with bilateral nephrectomy was performed. Case 3. : A 14 8/12 years old male child was diagnosed having chronic nephritis and chronic renal failure at 3 years old, progressed to end-stage renal failure (BUN/Cr 87/9.6 mg/dl) on OPD follow-up, and had a rephrectomy and renal transplantation. The biopsy specimen showed oligomeganephronic hypoplasia, secondary focal segmental glomerolosclerosis, and chronic interstitial nephritis. We report 3 cases of oligomeganephronia that progressed to end-stage renal failure and had successful renal transplantation with a brief review of related literatures.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.11-18
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• 2008

Anatomical characteristics of volumetric composition, fiber length, vessel diameter, and crystalline properties of cellulose in kenaf (Hibiscus cannabinus L.) planted in the reclaimed land of Buan-si, Korea were examined to understand the growth characteristics using a light microscopy and an X-ray diffraction method. The samples of kenaf were taken from six positions (3 cm, 35 cm, 70 cm, 105 cm, 280 cm, and 320 cm) of each stem over the growth period (July, August, September, and October) after seeding in the mid-May. In the kenaf stem, phloem constituted 10 to 15 %, xylem 66 to 82%, and pith 7 to 19%. The ray, bast fiber, and remainder comprised 50%, 20%, and 30% of the phloem, respectively. The volume of vessel, ray, and fiber in the xylem was approximately 10, 15, and 75%, respectively. The proportion of cell wall was 30.92% at the base of stem and 46.40% at the top of stem, respectively. The average length of bast fiber and xylem fiber was about 2.8 mm and 0.9 mm, respectively. Radial and tangential diameters of vessel increased with the increase of growth period, while they decreased with increasing the stem height. Relative crystallinity ranged from 70 to 79% in phloem and from 50 to 56% in xylem. Cellulose crystallite width was about 3 nm both in the phloem and xylem. Thus, the volumetric composition and cell dimensions in the phloem and xylem appeared to be varied with the growth period and the stem height.

• Journal of the Korean Wood Science and Technology
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• v.37 no.1
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• pp.19-28
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• 2009

Some wood anatomical characteristics of seven chestnut cultivars (Tanzawa, Ibuki, Arima, Ginyose, Tsukuba, Riheiguri, and Mansung) grown in Korea were examined in this study. We investigated the radial variation of wood components such as diameter and number of earlywood vessel element and ray density in cross section and number and height of ray in tangential section. Discs were taken from the stem of seven chestnut cultivars at breath height and cut into the radial strip. Cross, radial, and tangential sections, 10 to $20{\mu}m$ thick, were sliced with a sliding microtome. These sections were stained, dehydrated, and mounted with Canada balsam according to common procedure. Measurement and observation were performed with a light microscopy. In all chestnut cultivars, radial and tangential diameter of vessel element increased but number of vessel element per $mm^2$ decreased with increasing tree age. Ray spacing (per mm) in cross section from pith to bark showed no significant variation, while number of ray per $mm^2$ increased but ray height decreased with the increase of tree age. Conclusively, the results obtained in present study were thought to be some valuable information for separating juvenile and adult wood as well as identifying chestnut cultivars.

• Annals of Clinical Neurophysiology
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• v.1 no.2
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• pp.91-98
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• 1999

Background : The pathogenesis of acute inflammatory demyelinating polyradiculoneuropathy (AIDP), Guillain Barre syndrome (GBS) is not clear, but it has been known that the immune mechanisms play an important role. Authors performed this study to establish an animal model of experimental allergic neuritis (EAN) by immunizing the myelin components of peripheral nerves and to understand the electrophysiological and histopathological features as well as the ${CD_5}^+$ B-lymphocyte changes in peripheral bloods in the EAN models. Methods : Lewis rats weighing 150-200 gm were injected subcutaneously in soles two times with total myelin, P0, P1, or P2 proteins purified from the bovine cauda eguina. The EAN induction was assessed by evaluating clinical manifestations. The electrophysiological and histopathological features were studied as routine methods. The ${CD_5}^+$ Blymphocytes were double stained using monoclonal FITC conjugated anti-rat CD45RA and R-PE conjugated anti-rat ${CD_5}^+$ antibodies and calculated using a fluorescence activated cell sorter (FACS). Results : The EAN animal models were established. In two out of five, in one out of two, in none out of three, and in none out of one Lewis rats injected with purified total myelin, P0, P1, P2 proteins respectively, They showed slow spontaneous motor activity and weak resistance against pulling back by tails. The typical electrophysiological and histologic findings in total protein and P0 induced EAN animal models were the decreased conduction velocity, the decreased compound muscle action potential (CMAP) amplitude and the dispersion phenomenon. The perivascular infiltrates of lymphocytes with focal demyelinating process were found in light microscopy. The ${CD_5}^+$ B-lymphocyte expression in three EANs were 2.38%, 3.50% 2.50%, which were not significantly increased, compared with those in normal controls. Conclusion : The EAN animal models were successfully established by injecting the total myelin and P0 myelin and they showed electrophysiological and histological features typical of demyelinating process. However they did not show an increased expression of ${CD_5}^+$ B-lymphocyte in peripheral bloods which could be indirect evidence of humoral autoimmunity.

• Journal of the Mineralogical Society of Korea
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• v.5 no.1
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• pp.14-21
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• 1992

Allanite crystals rich in rare-earth elements(REE) occur in soil developed on top of anorthositic rocks in the Jungsu-ri area of Okjong-myun, Hadong-run, where large Ti orebodies are embedded in the bed rock. In this study allanite is investigated mainly by transmitted light microscopy, electron microprobe analysis, atomic absoption spectrophotometry, X-ray diffraction, infrared spectrocopy. In addition, its specific gravity and micro=indentation hardness value are measured. Allanite occurs with max. dimension of $3cm{\times}6cm$ and coexists with quartz, epidote, zircon, biotite and muscovite. It shows nearly nonmetamict crystallinity, although ${\alpha}$-particles bombardment from the disintegration of the radioactive element Th is detected by an autoradiography. The allanite is particularly enriched in REE(19.88-23.99 wt.%), but is deficient in CaO(8.35-10.29wt.%). Genesis of the allanite in this area is not understood yet. It is, however, assumed to have been formed from magmatic fluid rich in REE and Ti, based on the facts that it ocexists with zircon and that it has high $TiO_2$(0.89-1.13 wt.%) whose concentration is significant in the country rocks.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.371-377
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• 2012

This study was conducted to investigate the physiological and structural damages to cadmium (Cd) in Acorus calamus var. angustatus as a native aquatic species in Korea. In addition to the physiological responses such as plant growth, photosynthesis, and root activity, the structural damages in leaf and root tissues were observed through light and scanning electronic microscopy. The five-leaf plants were treated with different Cd concentrations 0, 10, 25, and 50 ${\mu}M$ for 15 days. The plant damages to Cd were significant at 10-25 ${\mu}M$ Cd physiologically and at 25-50 ${\mu}M$ Cd structurally. The physiological damages in the shoot part (photosynthesis) started at 10 ${\mu}M$ Cd whereas those in root part (root activity) were serious above 25 ${\mu}M$ Cd. On the other hand, the structural damages began at 25 ${\mu}M$ Cd in the leaf and root tissues similarly, but the plant tissue destruction was more serious in the roots than in leaves. Based on the plant physiological and structural damages, 10 ${\mu}M$ was assumed to be the limited concentration for sustainable growth and landscaping ability in Acorus calamus var. angustatus to Cd.