• 한국전기전자재료학회논문지
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• 제24권1호
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• pp.42-46
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• 2011

Light can be divided into ultraviolet rays, visible rays, and infrared rays depending on the wavelengths. Visible rays with specific wavelength are those predominantly used for would treatment. Especially low level laser irradiates into cells, effectively stimulating cellular tissues and activating cellular function. This study was intended to verify the effect of white LED irradiation therapy on wound recovery in animal tests by applying white LED irradiator, which was independently designed and developed to emit beams of similar wavelength to that of a laser. The designed LED Irradiator was used to find out how white LED light source affected the skin wound of SD-Rat(Sprague-Dawley Rat). We divided the participants into two groups; white LED irradiation group which was irradiated 1 hour a day for 9 consecutive days, and none irradiation group. The results showed that the study group had lower incidence of inflammation and faster recovery, compared with the control group.

• 한국식품과학회지
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• 제52권5호
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• pp.538-545
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• 2020

본 연구에서는 배추김치(RK)와 백김치(WK) 시료를 이용하여 72시간의 저장 기간 동안, Blue-LED 조사 효과를 확인하였다. BLED 처리에 의해 RK에서의 유산균 증식이 유의적으로 증가하였으나(p<0.05), 곰팡이 및 효모의 생육도에서는 대조구와 차이를 나타내지 않았다. 색도의 경우, BLED 처리 시 대부분 명도가 조금 높아지는 경향을 보였으며, BLED 조사에 의해 색의 변화가 일정기간 지연되는 효과를 확인할 수 있었다. BLED 처리에 의해 WK는 저장기간 동안 pH 감소가 유의적으로 지연되는 것을 관찰할 수 있었다(p<0.05). ABTS법에 의해 측정된 산화방지 활성에서는 두 종류의 김치시료액 모두 BLED처리구에서 유의적으로 높은 활성을 보였다(p<0.05). 두 김치에서 우점종으로 분리된 W. cibaria RK1와 W. cibaria WK1 유산균은 BLED에 민감하게 작용하여 모두 생육이 저해되었고, 표준 균주인 Leu. mesenteroides는 BLED 조사에 의한 생육도의 변화가 나타나지 않았다. 현재까지 BLED 조사를 이용한 유해 균주 억제 연구가 주로 이루어진 반면, 현 연구에서는 BLED조사에 의한 김치 발효상의 특성 변화와 발효미생물들의 생육도를 확인함으로써 발효 식품의 숙성 시기에 따라 적절한 LED 광원을 이용한 발효 미생물들의 생육 조절과 식품의 기능적 향상 가능성을 제시하였다.

• Transactions on Electrical and Electronic Materials
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• 제11권5호
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• pp.226-229
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• 2010

Many methods exist that promote wound healing, including light therapy, which consists of light beams that assist the human body in treating and sterilizing wounds, as well as regenerating cells. Irradiation with specific wavelengths of either laser or LED light has been shown to induce beneficial proliferation of fibroblasts that, depending on the size of the wound, can be effective in promoting wound healing. The experiments in this study utilized 8 week old 250~300 g Male Sprague Dawley Rats (ILAR Code: NTacSam:SD) and included a non-irradiation group and a 525 nm green LED irradiation group (n of each group = 7). In experiments animals were allowed to rest for 24 hours after wounds had been excised, which was followed by non- irradiation or 525 nm green LED irradiation therapy one hour per day for 9 days. Immunohistochemical staining was conducted for cytokeratin in order to precisely measure the defect size. In addition, Masson's trichrome staining was utilized in order to compare levels of collagen between the 525 nm green LED irradiation group and the non-irradiation group. Animals exposed to 525 nm green LED irradiation (p<0.05) healed at a faster rate and had increased collagenosis compared with the non-irradiated control group. Thus, treatment with 525 nm green LED irradiation had a beneficial effect on wound healing and should be considered as a possible alternative to low power laser treatment.

• 한국식품과학회지
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• 제45권6호
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• pp.763-769
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• 2013

본 연구는 전자코를 이용하여 시판 콩기름의 휘발성분이 파장에 영향을 받는지 알아보고자 암실과 형광등 및 파장이 다른 LED 광원을 PE 용기에 조사하면서 12주 동안 변화되는 휘발성분의 패턴 변화를 분석하였다. 콩기름의 휘발성분의 변화는 저장기간동안 암실 처리구에서 가장 적고 천천히 일어났으며, 형광등 처리구에서 가장 크고 급격한 변화가 일어나는 것을 보여주었다. 저장 초기인 4주에는 형광등 처리구를 제외한 모든 처리구에서 미세한 변화가 일어났으며, 저장 12주에는 형광등 처리구 다음으로 파란색과 백색 LED 처리구에서 큰 변화를 보여준 반면 초록색 LED 처리구에서는 변화가 적었다. 파란색과 흰색 LED 처리구는 저장 12주째에서 급격한 휘발성분의 변화가 나타났다. 하지만 빨간색과 초록색 LED 조사 시에는 8주째에 급격한 휘발성분이 생성된 것에 비하여, 저장 12주째에는 휘발성분의 변화가 매우 감소하는 것을 보여주었다. 또한 초록색 LED 처리구에서 가장 적은 변화를 보였다. 이러한 결과를 토대로 볼 때 초록색 LED 광원에서 콩기름을 전시 판매하는 것이 바람직하며, 형광등과 파란색, 백색 LED 광원은 바람직하지 못한 것으로 판단된다. 또한, 전시 판매가 아니라면 암실에서 저장하는 것이 가장 바람직할 것으로 예상되는 바이다.

• 한국전기전자재료학회논문지
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• 제21권8호
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• pp.760-765
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• 2008

Low power laser therapy is internationally certified and is known to be effective in stimulating DNA in living organisms, increasing protein synthesis and activating cell division, smoothing blood circulation, promoting cell activation, cell regeneration and function. It also has anti-inflammatory, anti-edemic, anti-fibrous dysplastic and neuralogic hyperfunctional effects. This study was intended to verify the effect of LED irradiation therapy on wound healing in cell and animal tests by applying LED irradiator using a laser and laser diode, which was independently designed and developed to emit beams of similar wavelength to that of a laser. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity and reservation. In case of cell proliferation experiment, each experiment was performed to irradiation group and non-irradiation group for tissue cells. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590 nm transmittance of micro-plate reader. In the wound healing experiment, 1$cm^2$ wounds on the skin wound of SD-Rat(Sprague-Dawley Rat) were made. Light irradiation group and none light irradiation group divided, each group was irradiated one hour a day for 9 days. As a result, the cell increase of tissue cells was verified in irradiation group as compared to non-irradiation group. And, compared with none light irradiation group, the lower incidence of inflammation and faster recovery was shown in light irradiation group.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.184-184
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• 2022

This study was aimed to evaluate the antioxidant activity and bioactive property of extracts from a purple-colored wheat variety 'Arriheuk' (Triticum aestivum L.) wheatgrass, affected by light emitting diode irradiation (LED). The wheatgrass was cultivated for 10 days after sowing in a growth chamber under the following LED conditions: R1B1 (Red:Blue = 1:1), R7B3 (Red:Blue = 7:3), and R3B7 (Red:Blue = 3:7). We examined antioxidant activity of the hot water extracts of wheatgrass using DPPH and ABTS free radicals scavenging assays. At the concentration of 10,000 ㎍/ml, the extract from R1B1 showed the highest DPPH free radical scavenging activity(79.29%), but its ABTS free radical scavenging activity was the lowest(32.0%). To evaluate bioactive property of the wheatgrass, we examined the change of natural killer (NK) cell activity affected by the wheatgrass extracts in vitro. At the concentration of 500㎍/ml, NK cell activity was most highly enhanced by the extract from R1B1(181.6%), and the activity was 176.1% (R7B3) and 144.6%(R3B7), respectively. These results suggest that the functional property of 'Arriheuk' wheatgrass would be enhanced by LED irradiation condition.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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• pp.416-417
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• 2008

This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. We developed the 4channel light medical therapy apparatus for external injury cure using a LED light source. This equipment was fabricated by using microcontroller, and designed to enable us to control light irradiation timer, intensity and reservation. In this paper, the designed device was used to find out how 470nm LEDs light source affects the skin wound of RAT. In the experiment, $1cm_2$ wounds on the External injury of RAT were made. Light irradiation RAT and none light irradiation RAT divided, each RAT was irradiated one hour a day for 15 days. In result, compared with none light irradiation RAT, the lower incidence of inflammation and faster recovery was shown in light irradiation RAT.

• 수산해양기술연구
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• 제50권4호
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• pp.542-550
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• 2014

We investigated biomass, diatom species and fucoxanthin contents as cell growth, fatty acid and amino acid contents as nutritional composition of diatoms attached on plate to confirm effects of light emitting diodes (LEDs) due to block off natural light. In the single LED irradiation, biomass showed significantly higher to $30.0{\pm}6.48mg/m^2$ in white LED than that of others (P<0.05). The dominate diatom species was Navicula cancellata. Their lipid contents showed significantly higher to $112.9{\pm}19.23ug/mg$ dry matter (DM) in control than that of others LEDs. But eicosapetaenoic acid (EPA) contents showed significantly higher to $3.3{\pm}0.62ug/mg$ DM than others, but not significantly differed with natural control light treatment (P<0.05). And total protein contents are higher in control and blue LED light than that of others, but essential amino acid contents showed significantly higher to $3.2{\pm}4.8%$ in control (P<0.05). In mixing light with natural and LED light, biomass showed $2.6{\pm}0.22mg/m^2$ in blue LED (P<0.05). Fatty acids contents were not significantly differed with all treatments. Amino acid contents showed to $11.0{\pm}0.33ug/mg$ DM in white LED (P<0.05), but not significantly differed with others LED lights (P>0.05). Therefore, we could suggest that irradiation of blue LED in natural light very benefit to diatom culture for larvae of sea cucumber and abalone and do on.

• Restorative Dentistry and Endodontics
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• 제47권1호
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• pp.7.1-7.14
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• 2022

Objectives: This study evaluated the bleaching efficacy of different in-office protocols associated with violet light emitting diode (V-LED), and measured the pulpal temperature rise caused by V-LED with or without gel application. Materials and Methods: Bovine incisors were distributed in 4 groups (n = 10): VL - V-LED; HP - 35% hydrogen peroxide (control); HYB - hybrid protocol, V-LED applied without gel for 10 irradiation cycles followed by V-LED applied with gel for another 10 irradiation cycles; and HPVL - gel and V-LED applied for 20 irradiation cycles. Three bleaching sessions were performed with 7-day intervals. Bleaching efficacy was evaluated with ΔE_ab^*, ΔE₀₀ and ΔWI_D. Data were recorded at baseline, 7, 14, 21 and 70 days. For pulpal temperature rise, thermocouples were placed inside the pulp chamber of human incisors. To determine intrapulpal temperature, the teeth were irradiated with V-LED with or without application of bleaching gel. Color difference data were analyzed by 2-way repeated measures ANOVA and Tukey's test. Pulpal temperature was analyzed by t-test (α = 5%). Results: VL exhibited lower color (ΔE_ab^* and ΔE₀₀) and whiteness changes (ΔWI_D) than the other groups. HPVL presented higher color change values than HYB. HYB and HPVL showed not different ΔWI_D values; and HP showed the highest whiteness changes at all times. There were significant differences comparing ΔT with gel (8.9℃) and without gel application (7.2℃). Conclusions: HPLV was more efficient than HYB. The 2 protocols with VL showed similar results to control. Gel application combined with VL promoted higher pulpal temperature than to the no gel group.

• 대한임상검사과학회지
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• 제47권1호
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• pp.6-10
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• 2015

Photodynamic therapy (PDT) apply photosensitizers and light. The purpose of this study was to evaluate the in vitro efficacy of PDT using blue LED (light emitting diode) with photofrin and radachlorin for Propionibacterium acnes. The colony forming units method was used to assess the antibacterial activity. Suspension (1 mL) containing P. acnes at $1{\times}10^5CFU/mL$ were prepared and then 2 fold serial diluted to $12.5{\mu}g/mL$ from $50{\mu}g/mL$ concentration of photofrin and radachlorin. After 60 minutes incubation, light was irradiated for 10 to 30 minutes using the following light source of wavelength 460 nm, each energy density 36, 72 and $108J/cm^2$. Bacterial growth was evaluated after 72 hours incubation in a Phenylethanol Blood Agar (PEBA) culture. In addition, flow cytometric analysis were performed to measure the live cell after PDT. Also transmission electron microscopy (TEM) was employed to evaluate the effect of pathogens by PDT. The PDT Group was perfectly killed to all kind of photosensitizers dose of $12.5{\mu}g/mL$ with irradiation of 10 minutes. Also other Groups were killed to all kind of photosensitizers dose of $6.25{\mu}g/mL$ with irradiation time of 20 and 30 minutes. The flow cytometry showed a lower number of viable bacteria in the PDT group compared to the control group. The images of the TEM results were showed in cytoplasmic membrane damage and partially deformed to cell morphologies. These results suggest that radachlorin and photofrin combine blue LED PDT can be effectively treated when was proved treatment for acnes therapy.