• Title/Summary/Keyword: Life-from

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Comparison of Alpha-Factor Preprosequence and a Classical Mammalian Signal Peptide for Secretion of Recombinant Xylanase xynB from Yeast Pichia pastoris

  • He, Zuyong;Huang, Yuankai;Qin, Yufeng;Liu, Zhiguo;Mo, Delin;Cong, Peiqing;Chen, Yaosheng
    • Journal of Microbiology and Biotechnology
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    • v.22 no.4
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    • pp.479-483
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    • 2012
  • The secretory efficiency of recombinant xylanase xynB from yeast Pichia pastoris between the ${\alpha}$-factor preprosequence and a classical mammalian signal peptide derived from bovine ${\beta}$-casein was compared. The results showed that although the bovine ${\beta}$-casein signal peptide could direct high-level secretion of recombinant xylanase, it was relatively less efficient than the ${\alpha}$-factor preprosequence. In contrast, the bovine ${\beta}$-casein signal peptide caused remarkably more recombinant xylanase trapped intracellularly. Real-time RT-PCR analysis indicated that the difference in the secretory level between the two signal sequences was not due to the difference in the transcriptional efficiency.

The Phenomenological Study on the Health and Life of Low-income Seniors who live in Poverty Area in Metropolitan City (달동네에 거주하는 저소득층 노인의 건강과 삶에 대한 현상학적 연구 - 광주광역시 발산마을 거주 노인을 중심으로)

  • Jang, Dongyeop;Shin, Heontae
    • Journal of Society of Preventive Korean Medicine
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    • v.21 no.2
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    • pp.79-94
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    • 2017
  • Objectives : The elderly in South Korea are the poorest among OECD countries in 2015. The aim of this study was to explore the health and life of the low-income elderly living in vulnerable areas in a metropolitan city. Methods : Data were collected through in-depth individual interviews with 7 participants from October to November 2015 and analyzed through Colaizzi's phenomenological methodology. The participants were interviewed for over 60 minutes in each person. Results : 7 categories were identified from 17 subcategories: "My life history: sick body," "Living with a sick body," "My poor but precious life," "A sense of distance from the hospital," "Narrowed area of my life," "Thankful for help," and "The village where I have lived my destiny." There is a lack of medical accessibility, mobility, and economic independence for low-income seniors. In addition, full-fledged redevelopment comes to them as violence. Conclusions : The health and life of the low-income elderly in vulnerable areas are products of many social factors, reaffirming the importance of social health.

The Influences of Conflict with Parents, Peer Relationship, and School Adaptation of Adolescents from Multicultural Families and their Satisfaction with Life : The Mediating Effects of Self-Esteem (다문화가정 청소년의 부모자녀갈등, 또래관계, 학교생활적응이 삶의 만족도에 미치는 영향 : 자아존중감의 매개효과를 중심으로)

  • An, Sun-Jung;Lee, Hyun-Chul;Lim, Ji-Young
    • Journal of Families and Better Life
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    • v.31 no.2
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    • pp.77-91
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    • 2013
  • The purpose of this study is to investigate if self-esteem mediates the effects of conflict with parents, peer relationship and school adaptation in relation to the satisfaction with life of adolescents in multicultural families. The subjects of the present study are 152 adolescents of multicultural families ranging from 12-16 years of age from the cities of Seoul, and Daegu, and the provinces of Gyeong-gi, and Gyeong-buk, South Korea. The measured constructs are Conflict with Parents, Friendship, School Adaption, Self- Esteem and Satisfaction with Life Scale. The dates were analyzed using path analysis. The major results of this study demonstrate that self-esteem does not mediate the effects of conflict with parents and peer relationship in relation to the satisfaction with life of adolescents in multicultural families. However, self-esteem does mediate the effects of school adaptation in relation to the satisfaction with life of adolescents in multicultural families.

A Competitive Advantage Strategy Based on Innovative Culture and Quality of Work Life: Evidence from SMEs of the Tourism Industry in Indonesia

  • HERMAWATI, Adya;ANAM, Choirul;SUWARTA, Suwarta;WARDHANI, Arie Restu
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.8
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    • pp.29-36
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    • 2022
  • The objective of this research is to find out the effect of innovative culture and quality of work life on competitive advantage strategy with the mediation of individual performance. This research is the continuance of previous research conducted by Adya Hermawati with an originality aspect emphasizing a concept comprising innovative culture, quality of work life, and individual performance as factors that control competitive advantage strategy. The research subject is Tourism Industry SMEs. Explanatory research is a research method used in this study, by surveying respondents. The data sources in this research are primary and secondary. Primary data is collected from respondents directly through a questionnaire whereas secondary data are obtained from references that are relevant to research problems. In conformity with this explanation, the type of research data is quantitative data. The results of this research show that: innovative culture has an effect on individual performance, quality of work life affects individual performance, innovative culture has an effect on competitive advantage, quality of work life affects competitive advantage, individual performance has an effect on competitive advantage, innovative culture affects competitive advantage with the mediation of individual performance, and quality of work life affects competitive advantage with the mediation of individual performance.

The Effect of Forest Experience on School Life Satisfaction of Korean Boarding Middle School Students

  • Myoung Suk Lee;Man-Leung Ha;Hyun Kim;Chong Kyu Lee
    • Journal of Forest and Environmental Science
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    • v.39 no.3
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    • pp.140-149
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    • 2023
  • We surveyed to assess the impact of the mood states and sentiments resulting from forest experience on the school life satisfaction of Korean boarding middle school students (n=90). The average mood state score was 2.32 on a scale of 1-5, with the subscale of vigor scoring the highest (3.19). The average sentiment score was 3.08, with the subscale of subjective happiness scoring the highest (3.08). The average school life satisfaction score was 3.66, with the subscale of interpersonal relationships scoring the highest (3.91). The mood states resulting from forest experience explained 39.6% of the variance in school life satisfaction, and the regression model showed a significant fit (F=9.077, p<0.001). Similarly, sentiments resulting from forest experience explained 47.4% of school life satisfaction, with the regression model showing a significant fit (F=10.569, p<0.001). It can be concluded that forest experience positively impacts the school life satisfaction of Korean boarding middle school students.

Attitudes and Awareness towards the Withdrawal of Life-Sustaining Treatment among Nurses, Physicians, and Families of Intensive Care Unit Patients (연명치료중단에 관한 중환자실간호사, 의사 및 중환자가족의 태도 및 인식)

  • Lee, Hyea Kyung;Kang, Hyun Sook
    • Journal of Korean Clinical Nursing Research
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    • v.16 no.3
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    • pp.85-98
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    • 2010
  • Purpose: This study was aimed to investigate the awareness and attitudes towards withdrawal of the life-sustaining treatment among nurses, physicians, and the families of intensive care unit (ICU) patients in general hospitals. Methods: The data were collected using a questionnaire from 80 ICU nurses, 80 physicians, and 80 families of ICU patients in general hospitals. Data were collected from February 22nd to May 31st, 2010. Rusults: ICU nurses, physicians, and families of ICU patients felt that objective and ethical guidelines were needed in making a decision to withdraw the life-sustaining treatment. The main reason for withdrawal of the life-sustaining treatment was found that the patients could not recovered despite many efforts. The role of nurses in decision making process on withdrawal of the life-sustaining treatment was considered very positive from the view of physicians and family members. The most important role of nurses for those patients in ICU was found to try their best to care for the patients. Conclusion: ICU nurses should play a major coordinating role in communication among patients, their families, and medical teams. Also, an appropriate roles of nurses in the process of the withdrawal of the life-sustaining treatment should be established.

Death Orientation of the Korean Adult - Data was focused on residents who were living in urban area - (대도시에 거주하는 한국인 성인의 죽음정위)

  • Kim Soon-Ja;Kil Suk-Yong;Park Chang-Seung
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.5 no.2
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    • pp.237-256
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    • 1998
  • Death and dying of human being is a comprehensive system, and death orientation, the subjective meaning related to every component of the death system is developed throughout life. This study was designed and carried out to identify, describe and classify the orientations of Korean adult towards the death system. In an attempt to measure the subjective meaning of death and dying, unstructured Q-methodology was used. The 65 Q-statements developed by Kim(1994), used by Kim(1994) and Park(1996) were adopted as Q-population and 39 Q-statements were selected by the three researchers for Q-items for this study. Thirty-three P-samples were sampled from P-population of literate Korean men and women, 35 and 55 years of age, lived in urban Korea for the last 10 years. Sortings of the 39 Q-items according to the level of personal agreement, and a forced normal distribution into the 9 levels were carried out by the P-samples. The Z-scores of the Q-sort data were computed, and the principal components factor analysis by PC-QUANL Program were carried out. The demographic, socio-cultural and health-related attributes of the P-samples were descriptively analysed. Eight types of death orientation were identified ; Type I ; 'naturalist'. Six P-samples. Death is a natural phenomena, to be accepted as it is and to follow its natural course. Prefer to be informed of all facts and possibilities concernig the course of dying and death to occur to self. Type II ; 'life-after-life negator'. Three P-samples. Time and process of death is the destiny of each person. Death means 'darkness' and 'end to every thing, the absolute end'. Yet, wish physical integrity at the dying and after death. Type III ; 'life-after-life believer'. Six P-samples. Men are travellers passing by this life bound to the life-after-life. Priority concerns are on the activities to prepare self for the eternal life ahead. Disregard premature and sudden death. Type IV ; 'here-now believer' Five P-samples. Positive regard to the cremation of the body and donation of the organs on death. Regard religious and customary post-motem rituals meaningless. Negate life-after life. Type V; 'believer of rituals'. Five P-samples. Death being accepted as a part of, a natural end to, and destiny of human life. Concerned to ensure a dignified end to personal life and dignified post-mortem rituals. Type VI ; 'Realist'(derived from Type I). Two P-samples. Life and death as universal reality. The abrupt death at golden age at the peak of happiness is favored to avoid inevitable physical and mental distress of self and the family. Agreed to the cremation of the body. Disregard rituals. Type VII : 'Fatalist' (derived from Type II). Five P-samples. Not favored, yet, all man are destined to death, the inevitable end of all living beings. To ensure dignified end by personal consummation, information on one's dying and imminent death are to be shared. Type VIII ; 'reality avoider'(derived from Type III). One P-sample. Negative to longevity, artificial prolongation of, meaningless and distressful life. Highly positive to postmortem organ donation.

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Social Support and Life Satisfaction of Living Alone elderly in Rural Area (농촌 독거노인들의 사회적 지원과 생활만족도)

  • Kim, Young-Soon;Yoon, Hee-Jung;Kwon, Jin-Hee;Moon, Hyo-Jung;Lee, Sung-Kook
    • Journal of agricultural medicine and community health
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    • v.27 no.1
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    • pp.65-78
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    • 2002
  • This study was conducted to identify the social support to living alone elderly in some rural communities and the level of life satisfaction. For the purpose of this study, face-to-face interviews and questionnaire were performed with 315 old people(male 43 and female 272) aged over 65 living alone in rural communities covered by 14 community health posts within Gyeongsangbuk-do Province randomly selected. The followings are summaries of findings; The average score of support from their children was $4.29{\pm}2.73$ out of 8. Variables that showed a significant difference were religion, level of living, type of medical insurance, frequency of meeting with children, time taken from houses of children by usual means of transportation, and subjective health status. It was found that the score of support from children was high for the elderly who had a religion, a good level of living, benefit from medical insurance, a high frequency of meeting with children, or a good subjective health status, or who resided close to their children's houses. The level of the support from friends and relatives showed a significant difference depending on the subjective health status, of which the average score was $4.13{\pm}2.61$ out of 8. The average score of the level of life satisfaction was $6.83{\pm}4.24$(male $7.60{\pm}4.09$ and female $6.71{\pm}4.26$) out of 17. Male elderly showed the higher level of life satisfaction than female elderly. Variables that showed a statistically significant difference in the level of life satisfaction were religion, level of living, medical insurance, hobby, children, disease, subjective health status, and ADL. That is, the level of satisfaction with life was found to be higher for the elderly who had a religion, a good level of living, benefit from medical insurance, a hobby, children, no diseases, or a good ADL, or who thought that they are healthy. The regression analysis with support from children as a dependent variable showed that the level of support from children was higher for the elderly who had a good level of living, frequency of meeting with children, or a good subjective health status. The regression analysis with the level of support from friends or relatives was higher for old people who had a good level of living. The regression analysis with the level of life satisfaction as a dependent variable showed that the factors which related to the level of satisfaction were sex, religion, level of living, hobby, ADL, and subjective health status. That is, it was found that for male elderly who had a religion, a good level of living or a hobby, or who thought that they were healthy, the level of life satisfaction was higher.

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Strength Evaluation and Life Prediction of the Multistage Degraded Materials (다단계 모의 열화재의 재료강도 평가와 수명예측)

  • 권재도;진영준;장순식
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.17 no.9
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    • pp.2271-2279
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    • 1993
  • In the case of life prediction on the structures and machines after long service, it is natural to consider a degradation problems. Most of degradation data form practical structures are isolated data obtained at the time of periodical inspection or repair. From such data, it may be difficult to obtain the degradation curve available and necessary for life prediction. In this paper, for the purpose of obtaining a degradation curves, developed the simulate degradation method and fatigue test and Charpy impact test were conducted on the degraded, simulate degraded and recovered materials. Fatigue life prediction were conducted by using the relationship between fracture transition temperature (DBTT : vTrs) obtained from the Charpy impact test through the degradation process and fatigue crack growth constants of m and C obtained from the fatigue test.

Stress, Social Support, and Maladjustment of Adolescents (청소년의 스트레스와 사회적 지원 및 부적응)

  • Sim, Hee Og
    • Korean Journal of Child Studies
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    • v.16 no.1
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    • pp.89-98
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    • 1995
  • The present study explored a factor, social support, that could mediate the relationship between negative life events and behavioral problems during adolescence. It was hypothesized that social support from parents, peers, and teachers would be an effective mediator between negative life events and delinquent behavior. Data were collected from 161 of the 9th graders in a high school in a coastal community of Oregon. A series of path analyses using regression techniques was used to examine the research hypotheses. Results indicated that social support mediated the relationship between negative life events and delinquent behavior, implying the importance of social support from family, peers, and school in preventing delinquent behavior among adolescents. That is, negative life events were found to exert influence indirectly upon delinquent behavior through its direct effect on social support. Results were discussed in relation to the effects of negative life events on social support and delinquent behavior.

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