• Applied Biological Chemistry
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• v.39 no.2
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• pp.104-111
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• 1996

We have isolated several proteinase inhibitor II genes pin2 from a Russet Burbank potato DNA library. One of these, pin2T was subcloned and a 1.8 kb Xbal/Nsil insert was sequenced. This fragment contained the complete Inhibitor II gene including 965 Up of flanking DNA upstream from the gene and 200 bp of flanking DNA downstream from the gene. The open reading frame encodes a protein that is similar to other reported proteinase Inhibitor II proteins. The DNA sequence of the 5' flanking region of pin2T from -714 to +1 is highly homologous (91% identity) with that of the previously isolated wound-inducible pin2K. There are, however, four small deletions in the pin2T promoter which are located at -221 to -200, -263 to -254, -523 to -426 and -759 to -708 relative to the transcription start site of the wound-inducible pin2K. Three of these deletions map to a portion of the promoter that controls the wound-inducibility of the proteinase inhibitor genes. Chimeric genes containing the promoter of the pin2T gene linked with the both CAT and GUS were constructed and transfered into tobacco plants. Analysis of these plants indicated that pin2T is not a wound-inducible gene but is expressed at low levels. Thus, wound-inducibility is lost with the concomitant natural deletion of three small regions of the promoter. Comparision of the sequences deleted in pin2T relative to the pin2K with Genebank sequences indicates that the deleted sequences contain a motif (consensus 5'-AGTAAA-3') that is found in many other wound-inducible genes but not easily found in the published promoter sequences of other plant genes. Nuclear proteins from unwounded and wounded potato leaves were bound to the proximal promoter region, downstream of the 5'-AGTAAA-3', of pin2T. The comparison of the pin2T gone with the pin2K gene indicates that the natural internal promoter deletions are likely responsible for loss of the wound-inducible phenotype in the pin2T gene.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.55-62
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• 1995

To understand the molecular structure of Korean garlic viruses, cDNA cloning of virus genomic RNA was attempted. Virus particles were isolated from virus-infected garlic leaves and a cDNA library was constructed from garlic virus RNA. One of these clones, S81, selected by random sequencing has been identified as a member of potexvirus group other than potyvirus and carlavirus. The clone is 873 bp long contains most of the coat protein (CP) coding region and 3'-noncoding region including poly(A) tail. A putative polyadenylation signal sequence (AAUAAA) and the hexanucleotide motif (ACUUAA), a replicational cis-acting element conserved in the 3'-noncoding region of potexvirus RNAs are noticed. The clone S81 shows about 30-40% identity in both nucleotide and amino acid sequences with CPs of potexviruses. The genome size of the virus was analysed to be 7.46 knt by Northern blot analysis, which was longer than those of other potexviruses. The open reading frame encoding CP was expressed as a fusion protein (S81CP) in Escherichia coli and the recombinant protein was purified by immobilized metal binding affinity chromatography. Polyclonal antibody was raised against S81CP in rabbit to examine the occurrence of garlic potexvirus in Korean garlic plants by immunoblot analysis. Two virus protein bands of Mr 27,000 and 29,000 from garlic leaf extract of various cultivars reacted with the antibody. It was shown that Mr 27,000 band might not be a degradation product of Mr 29,000 band, suggesting that two types of potexvirus different in size of coat protein could exist in Korean garlic plants.

• Radiation Oncology Journal
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• v.17 no.2
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• pp.151-157
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• 1999

Purpose : By sequencing the Erpressed Sequence Tags of human 걸ermal papilla CDNA library, we identified a clone named K872 of which the expression decreased during differentiation of HL6O cell line. Materials and Methods : K872 plasmid DNA was isolated according to QIA plasmid extraction kit (Qiagen GmbH, Germany). The nucleotide sequencing was performed by Sanger's method with K872 plasmid DNA. The most updated GenBank EMBL necleic acid banks were searched through the internet by using BLAST (Basic Local Alignment Search Tools) program. Nothern bots were performed using RNA isolated from various human tissues and cancer cell lines. The gene expression of the fusion protein was achieved by His-Patch Thiofusicn expression system and the protein product was identified on SDS-PAGE. Results : K872 clone is 1006 nucleotides long, and has a coding region of 675 nucleotides and a 3' non-coding region of 280 nucleotides. The presumed open reading frame starting at the 5' terminus of K872 encodes 226 amino acids, including the initiation methionine residue. The amino acid sequence deduced from the open reading frame of K872 shares $70\%$, identity with that of rat glutathione 5-transferase kappa 1 (rGSTKl). The transcripts were expressed in a variety of human tissues and cancer cells. The levels of transcript were relatively high in those tissues such as heart, skeletal muscle, and peripheral blood leukocyte. It is noteworthy that K872 was found to be abundantly expressed in coloreetal cancer and melanoma cell lines. Conclusion : Homology search result suggests that K872 clone is the human homolog of the rGSTK1 which is known to be involved in the resistance of cytotoxic therapy. We propose that meticulous functional analysis should be followed to confirm that.

• Korean Journal of Social Welfare
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• v.57 no.4
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• pp.147-175
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• 2005

Efforts to identify the public's perception of social welfare as an academic discipline have never been conducted in Korea since the establishment of social welfare department in 1947 at Ewha Womans University. Such efforts are very meaningful in identifying directions and tasks to strengthen Korean social welfare as well as in clarifying and promoting our understanding concerning status of the academic discipline. This study attempts to explore and describe the degree of the public's perception in Korea with analyzing data surveyed in 2004 by our interdisciplinary research team. This study develops and uses a questionnaire having a Likert scale format that is composed of 8 points and measures the public's perception in the following dimensions: (1) personal interests on academic discipline; (2) contribution of academic discipline; (3) prospect of academic discipline; (4) importance of academic discipline; (5) expertise of academic discipline; and (6) personal knowledge on academic discipline. To avoid social desirability and promote objectivity with comparative measurement, this study selects ten representative academic disciplines as follows: medicine; physics; biology; social welfare; economics; psychology; sociology; political science; library science; and communication & journalism. This study attempts to identify (1) the degree of the public's perception on ten academic disciplines; (2) the position of social welfare by comparing it with each academic discipline and by comparing mean of social welfare with overall mean of six social science disciplines in the six dimensions; (3) the differences in the public's perceptions of social welfare on six dimensions by the respondents' status factor(high school students, college and graduate students, and citizens) and gender factor by using MANCOVA, and (4) the differences in the public's perceptions of social welfare on six dimensions by major factor(social welfare, social science majors, and natural science majors) and gender factor of college and graduate school students by using MANCOVA. The results of data analysis are as follows: (1) while the 3,319 respondents gave relatively high rating on natural sciences in the dimensions of contribution and expertise, they did the same on social sciences in the dimensions of personal interests and personal knowledge; (2) in overall comparisons, while the 3,319 respondents gave relatively high rating on social welfare in the dimensions of contribution, prospect and importance, they gave the lowest rating on the expertise of social welfare; (3) in the comparisons with social science disciplines, while the 3,319 respondents gave relatively high rating on social welfare in the dimensions of contribution, prospect and importance, they gave the lowest rating on the expertise of social welfare; (4) when analyzing all the respondents, there were differences in the vector of personal interests, contribution, prospect, importance, expertise, and personal knowledge by status factor, gender factor, and interaction effect factor; and (5) when analyzing only the respondents in college and graduate schools, there were differences in the vector of personal interests, contribution, prospect, importance, expertise, and personal knowledge by only major factor and gender factor. The results provide empirical backgrounds for discussing current image, status and major characteristics of social welfare as a discipline in Korea. Indeed, this study provides new meaningful and thoughtful guide for further investigation on the topic. In addition, contributing to clarifying and broadening our understandings about the public's perception on social welfare in Korea, this study discusses the tasks for dealing with expertise issue that is the most vulnerable issue of Korean social welfare discipline and research directions to strengthen and promote social welfare discipline in Korea.