• Journal of Fashion Business
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• v.15 no.3
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• pp.51-66
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• 2011

The purpose of this study is to investigate the effect of the traditional color sentiment on the contemporary clothing color by studying the pictures of street fashion of Dalian in China, in view of the clothing color of women in their 20-30 years of age having highest purchasing power, along with traditional Chinese color. The clothing color is various in frequency depending on the items in street fashion. Due to the seasonal impact of summer, the most frequent item was one-piece dress with the Multi of various color patterns, followed by White, Black, PB, R and B category. In the top color, the White was most prevalent color due to the seasonal factor, followed by Black, Grey, Multi, R, Y, RP category. Achromatic color is more dominant with the ratio of 7:3, comparing with its counterpart, which consists of R and Y category of V, B, P, VP tone. In the bottom color, Dp tone of PB shows most high frequency, followed by Black. This results illustrate that Chinese women prefer blue jeans and to be looked as slimmer by using of the dark colors. In the accessories, colors of bags and shoes show different results. The bag colors show the high frequency of Dk tone, YR category, and the chromatic colors are little bit dominant than achromatic ones with the ratio of 5.3:4.7. On the contrary, the shoes colors show the highest frequency in Black, the achromatic colors are more dominant than chromatic ones with the ratio of 6.6:3.4. These results somewhat diverge from the international color trend. Color trend in Dalian street fashion in which the high frequency of V tone is observed through all the colors of the items followed by P, VP, Lgr tone in sequence. In the light of Chinese traditional color preference, this result denotes that the traditional color preferences of red, yellow are still affecting the contemporary color choices of clothing in Chinese women. The high incidence of PB category in the bottom and one-piece dress does not seem to have its origin form traditional Chinese color sentiment.

• Journal of Veterinary Science
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• v.24 no.4
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• pp.53.1-53.12
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• 2023

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied. Objectives: This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an in vitro model for intestinal MRV3 infection. Methods: PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as Lgr5, LYZI, Mucin-2, ChgA, and Villin. The monolayered PIOs and three-dimensional (3D) PIOs, obtained through their distribution to expose the apical surface, were infected with MRV3 for 2 h, washed with Dulbecco's phosphate-buffered saline, and observed. Viral infection was confirmed using PCR and IFA. We performed quantitative real-time reverse transcription-PCR to assess changes in viral copy numbers and gene expressions linked to intestinal epithelial genes and antiviral activity. Results: The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. Conclusions: We suggest that PIOs can be an in vitro model to study the infection mechanism of MRV3 in detail, facilitating pharmaceutical development.