• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.488-494
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• 1999

Hydrolytic peptides of salted and fermented anchovy sauce, and anchovy actomyosin for the development of a rapid fermentation method with conventional tastes and flavors were studied. The optimal temperatures of crude enzymes isolated from anchor, liver and viscera of squid were 55, 40$\~$45 and $45\~60^{\circ}C$, respectively. Crude enzyme isolated from anchovy was more effective on hydrolysis of anchovy actomyosin than that from squid liver and viscera. But the crude enzyme from squid liver was less effective on NaCl than that from anchovy. Three peptides occurred in anchovy actomyosin hydrolyzed with crude enzymes from anchovy and squid liver for 30 min. Their molecular weight were determined by Superdex 200 gel chromatography as 10,800, 5,800 and 2,600 dalton. When anchovy sauce was hydrolyzed with crude enzymes of anchovy, squid liver and viscera, and Protamex during 70 days, ranges of their low molecular weight of hydrolyzed peptides were 300$\~$1,000dalton detected by Sephadex G-50 and their major amino acid compositions were glutamic acid, glycine and alanine, which was related with conventional tastes. Those amino acid compositions were similar to those of anchovy sauce hydrolyzed with squid liver, In the case of Protamex treatment, hydrolyzed peptides had high levels of isoleucine and leucine, being associated with the bitter, but a low level of glutamic acid.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.416-419
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• 1999

To evaluate lipid oxidation in dried shellfish during storage, brown pigment formation and composition of fatty acid were determined. Levels of brown pigment formation occurred at $25^{\circ}C$ were higher than those occurred at $4^{\circ}C$ throughout the storage period. Especially, the formation content of lipophilic brown pigment was 9$\~$10 times higher than that of hydrophilic brown pigment in all samples. Fatty acid composition of total lipid (TL) in fresh sea mussel and baby clam were $34.21\%$ and $32.83\%$ in saturated fatty acid, $19.50\%$ and $20,83\%$ in monoenoic acid, and $46.29\%$ and $46.79\%$ in polyenoic acid, respectively. The prominent fatty acids were 16:0, 16:1 and 20:5 in TL of the both samples. During storage, the contents of saturated fatty acid and monoenoic acid were slightly increased but that of polyenoic acid was decreased. In sea mussel, the content of polyenoic acid was higher in phospholipid (PL) than in neutral lipid (NL), while saturated fatty acid and monoenoic acid content were higher in the latter. The ratios of polyenoic acid/saturated fatty acid (P/S) in NL was similar in all samples. But the ratio of P/S in PL was higher in baby clam ($1.81\%$) than that in sea mussel($1.42\%$).

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.958-964
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• 2004

This study examined the effects of maternal calcium intake levels on mineral metabolism during pregnancy and lactation. Five week-old female Sprague-Dawley rats were fed normal Ca (NCa, 0.5%) and low Ca (LCa, 0.15%) diets for 5 weeks during preconception. The low Ca intake group was divided into three groups and fed low Ca (0.15%), normal Ca (0.5%) and high Ca (1.5%) diets for 6 weeks during pregnancy and lactation. All of the rats were mated with normal male rats. The control group was consistently fed a normal Ca diet for 11 weeks. Pups were adjusted to 10 per dam. After weaning, dams and their weaned rats were sacrificed. We measured total protein, albumin, alkaline phosphatase and mineral content in serum and weight, length, breaking force, ash and mineral content in bone and kidney. There was no significant difference in weight gain and food intake among the groups. Serum total protein and albumin were in the normal range but slightly decreased with a low Ca diet during preconception. Calcium supplementation above normal level increased bone weight, Ca content and breaking force in femur of maternal and their weaned rats. However, high maternal Ca intake significantly decreased Mg and Fe contents in maternal serum and bone and Mg and Zn contents in serum of weaned rats. Also, it induced Ca, P and Mg deposit in maternal kidney. Our experimental results suggest that Ca deficiency during preconception may deteriorate bone status of mother and their offsprings. Moreover, high Ca supplementation during pregnancy and lactation may lead to disturb mineral metabolism in bone and other tissues.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.9
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• pp.1185-1193
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• 2006

This study investigated the effects of green tea on the disorders of lipid metabolism, oxidative system and hepatic functions induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), using adult male rats (SD) for 3 weeks. These 36 animals were divided into four groups. TCDD ($50{\mu}g/kg$ BW) was intraperitoneally injected at the beginning of experiment. Green tea powder was added 1% or 3% levels in basal diets respectively. Relative weights of thymus were decreased about one-third of control group, but those of liver, brain and testis were significantly increased in rats treated TCDD. Neutrophill% and lymphocyte% by TCDD treatment was improved by green tea diets. In liver functional enzyme, elevation of glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (ALP) activities due to TCDD treatment was lowered by green tea diets. The concentrations of serum and liver lipids were significantly increased by TCDD treatment, however, those of serum and liver triglyceride tended to decrease by green tea diets. Fecal lipid excretion was increased in rats fed green tea diets. Especially, fecal total cholesterol level was significantly elevated by 3% green tea diets. The activities of superoxide dismutase (SOD) were increased in rats fed 3% green tea diets. Increment of benzphetamine N-demethylase (BPND) activity by TCDD treatment was declined by 1% green tea diets. These results indicate that green tea can exert improving effects on liver lipid accumulation and unfavorable hepatic functions, and elevate antioxidation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.9
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• pp.1207-1214
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• 2006

The purposes of this study were to determine the estimated dietary fiber (DF) intakes per capita/day of Korean as of 2001 by age groups and sex, and to analyze the major food sources of DF using the data on per capita consumption of each food reported in the 2001 Reports of Korean National Nutrition Survey and the newly-established DF database. The mean daily intakes of DF of Korean increased with increasing age until 49 years and thereafter decreased with aging. The intakes of DF/1,000 kcal increased with increasing age until 64 years and thereafter decreased with aging. On the average, the Korean people under age of 49 did not meet adequate intake (AI) of DF (12 g/1,000 kcal) in Dietary Reference Intakes (DRI) for the Koreans. The male people less satisfied the AI than the female did. The major food groups contributed to DF intakes of Korean adults were high in the order of vegetables, cereals, fruits, seasonings, legumes and seaweeds. Regardless of age and sex, vegetables, cereals and fruits were three major sources of DF for Koreans who obtained approximately 75% of DF from those sources. It has been shown that rice was the first single food source of DF intake for the age groups less than 12 years, however Kimchi was the first major source for all the age groups over 12 years. The infant and elderly groups obtained more DF from 10 major single food sources than the other age groups did. The results of the study revealed that the present levels of DF intakes of Korean in all age groups except the elderly group are insufficient to meet the AI for DF. Therefore the beneficial effects of DF on health and the increased consumption of DF from the variety of food sources should be emphasized through the continuous nutritional education.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.7
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• pp.853-859
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• 2006

This study was carried out to investigate the effect of egg shell calcium salt types and egg shell membrane on calcium metabolism in rats. Sprague-Dawley male rats, 4 weeks of age, were fed on free-calcium diets for 2 weeks after adjustment period. Rats weighing approximately $247{\pm}2.3g$ were divided into 6 groups and were fed on the experimental diets containing 0.2% calcium for 4 weeks. Experimental groups were as follows; {ES(M+)} (egg shell powder diet with egg shell membrane), {ES(M-)} (egg shell powder diet without egg shell membrane), {AC(M+)} (egg shell calcium acetate diet with egg shell membrance), {AC(M-)} (egg shell calcium acetate diet without eg shell membrane), {GC(M+)} (egg shell calcium glucuronate diet with egg shell membrane) and {GC(M-)} (egg shell calcium glucuronate diet without egg shell membrane). Bone length of femur was significantly different by the types (p<0.05) of egg shell calcium salts. Bone mineral density of femur showed the highest level in AC(M-) group. Calcium content of femur and calcium absorption rate were higher in egg shell calcium salt groups than in eg shell powder groups. Calcium absorption rate and retention were significantly different (p<0.05) among the types of eg shell calcium salts and were higher in the AC(M-) group than in the other groups. Alkaline phosphatase activity, parathyroid hormone and osteocalcin levels of serum showed no significant difference among the experimental groups. From the above results, it is concluded that bioavailability of calcium is higher in groups of egg shell calcium salts compared to those in egg shell powder, even though egg shell membrane has no effect on calcium metabolism. Thus, these findings suggest the possibility of using egg shell calcium salts as a functional food material related to calcium metabolism.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.7
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• pp.860-865
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• 2006

This study was performed to investigate the effect of ethanol and citric acid-treated anchovy, caseino-phosphopeptides (CPPs), calcium lactate, and calcium phosphate as dietary calcium supplements on calcium metabolism in rats for 5 weeks. Experimental animals were randomly assigned to five treatments with 15 heads of SD male rats (mean body wt. of 100 g) in each group. The experimental diets were as follows; dried large anchovy powder (C) as control, ethanol+citric acid group (EC), ethanol+citric acid+cpps group (ECC), calcium lactate group (CL) and calcium phosphate group (CP), which were formulated with commercial semi-purified Chow diet, while maintaining the same level of calcium in all diets (1%) groups. The weight gain of EC group was significantly higher than ECC, CL and CP groups (p<0.05), food efficiency (FER) was not different. In vitro and in vivo calcium absorption rates of ECC group treated with citric acid and CPPs were 20.4 and 28.4%, respectively, and the highest among the experimental groups (p<0.05). The blood glucose levels of CL group (105.7 mg/dL) was significantly higher than control group (98.5 mg/dL). In terms of serum lipids, total-cholesterol concentration of EC group (75.1 mg/dL) was significantly higher than CP group (65.6 mg/dL) and triglyceride concentration of CP group (33.5 mg/dL) was the lowest (p<0.05). ALP activity and 057 level were not different among experimental groups. The serum calcium concentration of control group (C) was the lowest among groups (p<0.05). The femur weight of CP group was the lowest (p<0.05) and the femur length of ECC group is the longest (P<0.05). The bone density of CP group $(0.1116\;g/cm^2)$ was the lowest while ECC group $(0.1149\;g/cm^2)$ was the highest, and the bone density was increased by added CPPs. These data demonstrated that ECC group significantly increased in vitro and in vivo calcium absorption rate, serum Ca level, and the length and bone density of femur.

• Journal of Korean Society of Forest Science
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• v.47 no.1
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• pp.1-15
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• 1980

The occurrence of witches' broom of Rhus javanica was first noticed in Korea by the author in 1979. Subsequently, studies were made on the symptomatology, etiology, and transmission of the disease, as well as the effect of some antibiotics on the disease development. The results of these studies are summarized as follows: 1. Symptoms of the infected plant were characterized by dwarfing of the tree accompanied by yellowing and brooming of the foliage. 2. Electron microscopy of witches' broom diseased Rhus javanica plant revealed the occurrence of numerous mycoplasma-like organisms (MLO's) in the phloem tissue cells (sieve tube elements and phloem parenchyma cells) of the rachis and midribs of infected leaves. 3. The MLO's were bounded by a single unit membrane and contained ribosome-like granules and strands presumed to be DNA. It also appears that the MLO multiply possibly by budding as well as binary and plurinary fission. 4. In the midrib of healthy leaves, vascular bundles were collaterally discontinuous. In the diseased leaves, however, xylems were connected to each other and phloem cells showed an atrophy. Granules, which were prominent in the normal abaxial epidermis, were not observed in the peidermis of diseased leaves. 5. Electron microscopy revealed crystals or osmopholic granules in the phloem parenchyma cells, and that normal stacks of grana were not developed in the chloroplasts of infected levels. 6. The disease was experimentally transmitted by grafting. Budding was more effective than crown grafting for transmitting the disease. The disease has been transmitted by grafting even when complete union of stocks and scions has not taken place. The disease agent was not transmitted by sap inoculation. Insect transmission has not been confirmed. 7. Dipping the roots of infected plants into the 500 ppm and 1,000 solutions of either tetracycline HCI or oxytetracycline, HCI was more effective on temporary remision of the symptoms than spraying the 100 ppm and 200 ppm solutions of the same antibiotics. A greater effect was achieved through dipping into 1,000 ppm than into 500 ppm.

• Journal of Korean Society of Forest Science
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• v.76 no.4
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• pp.397-409
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• 1987

This study was executed to analyze the user's impact on vegetation structure changes and user's psychology in Odongdo Island of Hallyo-Haesang National Park. Five sites were sampled for vegetation structure changes vi the study area according to the extent of impact observed. Also user's psychology was studied through questionnaire with the visitors and 366 answers were collected at random sampling in May, 1986. Evergreen broad-leaved forest, i.e. Machilus thunbergii, Cinnamomum camphora, and Camellia japonica forest, took possession of 32.5% (3.91ha) of total forest area when condisering the actual vegetation. Camellia japonica community covered 40.0% (4.72ha) and Sasa coreana community took possession of 41.8% (5.02ha). The area of environmental impact grade 3 and 4 area covered 44.3% of total forest area and it should be restored because self-repair seemed to be impossible. The evergreen broad-leaved forest was destoryed seriously with no younger trees in middle and lower layers by overuse impact and would be bared soon. So the preservation of autochthonous flora is required by the control of the number of users. It was shown that most of visitors come on holidays and Sunday and places which were favorably impressed were shown as the area of showing the sea and Camellia forest. Overall levels of satisfaction was comparatively low, consequently 55% of visitors were satisfied. This level of satisfaction was associated with number of users, landscape of forest and number of facilities.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.420-436
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• 2016

The purpose of this study was to identify the status of task performance and working conditions for nutrition teachers in order to determine new ways to improve the current nutrition system and increase job satisfaction among teachers. From the 14th of July to the 26th of September 2014, email questionnaires were distributed to and collected from 311 nutrition teachers nationwide, including teachers at elementary, middle, and high schools. The results are as follows. First, over 90% of nutrition teachers indicated that their work was demanding. In particular, 63.5% of nutrition teachers at high schools worked more than 40 hours of overtime per month. Second, 73% of nutrition teachers provided nutrition education, but the percentage significantly decreased at upper levels of school. Nutrition teachers had difficulties teaching due to the absence of standard teaching materials and a lack of time due to excessive work. Teachers also wanted 30 hours of job training once per year during their vacation organized by the regional Department of Education. Third, around half of the nutrition teachers considered that promotion and transfer professions are necessary for systematic foodservice and education. An additional allowance was demanded by nutrition teachers at schools that serve two or three meals per day. Considering the results, alleviating the workload of nutrition teachers and more time preparing nutrition classes for student are required. Fair evaluation of job performance that considers work conditions of nutrition teachers should be considered.