• Title/Summary/Keyword: Leuconostoc mesenteroides subsp. cermoris DLAB19

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Antimutagenic Effects against N-methyl-N`-nitro-N-nitrosoguandine and 4-nitroquinoline-1-oxide on Cultrue Conditions of Leuconostoc mesenteroides subsp. cremoris DLAB19 isolated from Dongchimi (동치미에서 분리한 Leuconostoc mesenteroides subsp. cremoris DLAB19의 배양 조건에 따른 N-methyl-N`-nitro-N-nitrosoguandine과 4-nitroquinoline-1-oxide에 대한 항돌연변이 효과)

  • Rhee, Chang-Ho;Joo, Gil-Jae;Woo, Cheol-Joo
    • Journal of Life Science
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    • v.11 no.5
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    • pp.439-446
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    • 2001

  • Leuconostoc mesenteroides subsp. cremoris DLAB19 were investgated under various culture conditions to maximize the production of antimutagenic substance(s) against N-methyl-N\`-nitro-N-nitrosoguandine(MNNG) on Salmonella enterica serovar typhimurium TA100 and 4-nitroquinoline-1-oxide(4-NQO) on S. enterica serovar typhimurium TA98. The MRS medium containing glucose (2%) as a carbon source and yeasty extract (1%) as a nitrogen source resulted in the highest production of the antimutagenic substance(s) against both mutagens in the culture supernatant of Leu. mesenteroides subsp. cremoris DLAB19. Optimal pH of the culture medium, culture temperature and shaking speed for the antimutagenic substance(s) production were pH 7.0, 3$0^{\circ}C$ and 150 rpm, respectively. Under the optimal condition, the antimutagenic effects of Leu. mesenteroides subsp. cremoris DLAB19 culture supernatant were 96.4% against MNNG on S.enterica serovar typhimurium TA100 and 53.8% against 4-NQO on S. enterica serovar typhimurium TA98.

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