• Tuberculosis and Respiratory Diseases
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• v.45 no.4
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• pp.861-869
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• 1998

Backgrounds: The injury of a tissue results in the infalmmation, and the imflammed tissue is replaced by the normal parenchymal cells during the process of repair. But, constitutional or repetitive damage of a tissue causes the deposition of collagen resulting in the loss of its function. These lesions are found in the lung of patients with idiopathic pulmonary fibrosis, complicated fibrosis after diffuse alveolar damage (DAD) and inorganic dust-induced lung fibrosis. The tissue from lungs of patients undergoing episodes of active and/or end-stage pulmonary fibrosis shows the accumulation of inflammatory cells, such as mononuclear cells, neutrophils, mast cells and eosinophils, and fibroblast hyperplasia. In this regard, it appears that the inflammation triggers fibroblast activation and proliferation with enhanced matrix synthesis, stimulated by inflammatory mediators such as interleukin-1 (IL-1) and/or tumor necrosis factor (TNF). It has been well known that TGF-$\beta$ enhance the proliferation of fibroblasts and the production of collagen and fibronectin, and inhibit the degradation of collagen. In this regard, It is likely that TGF-$\beta$ undergoes important roles in the pathogenesis of pulmonary fibrosis. Nevertheless, this single cytokine is not the sole regulator of the pulmonary fibrotic response. It is likely that the balance of many cytokines including TGF-$\beta$, IL-1, IL-6 and TNF-$\alpha$ regulates the pathogenesis of pulmonary fibrosis. In this study, we investigate the interaction of TGF-$\beta$, IL-1$\beta$, IL-6 and TNF-$\alpha$ and their effect on the proliferation of fibroblasts. Methods: We used a human fibroblast cell line, MRC-5 (ATCC). The culture of MRC-5 was confirmed by immunofluorecent staining. First, we determined the concentration of serum in cuture medium, in which the proliferation of MRC-5 is supressed but the survival of MRC-5 is retained. Second, we measured optical density after staining the cytokine-stimulated cells with 0.5% naphthol blue black in order to detect the effect of cytokines on the proliferation of MRC-5. Result: In the medium containing 0.5% fetal calf serum, the proliferation of MRC-5 increased by 50%, and it was maintained for 6 days. IL-1$\beta$, TNF-$\alpha$ and IL-6 induced the proliferation of MRC-5 by 45%, 160% and 120%, respectively. IL-1$\beta$ and TNF-$\alpha$ enhanced TGF-$\beta$-induced proliferation of MRC-5 by 64% and 159%, but IL-6 did not affect the TGF-$\beta$-induced proliferation. And lNF-$\alpha$-induced proliferation of MRC-5 was reduced by IL-1$\beta$ in 50%. TGF-$\beta$, TNF-$\alpha$ and both induced the proliferation of MRC-5 to 89%, 135% and 222%, respectively. Conclusions: TNF-$\alpha$, TGF-$\beta$ and IL-1$\beta$, in the order of the effectiveness, showed the induction of MRC-5 proliferation of MRC-5. TNF-$\alpha$ and IL-1$\beta$ enhance the TGF-$\beta$-induced proliferation of MRC-5, but IL-6 did not have any effect TNF-$\alpha$-induced proliferation of MRC-5 is diminished by IL-1, and TNF-$\alpha$ and TGF-$\beta$ showed a additive effect.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.2
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• pp.47-58
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• 1981

Relationships between the electrical conductivity and the contents of the chloride, sulfate, calcium, magnesium, sodium, potassium and total major inorganic ions, and between each, chemical conservative constituents were calculated with the data which sampled at the lesions of Mulgeum and between Namji and Wondong from March 1974 to April 1980. Semilogarithmic relations were found between the electrical conductivity and the contents of monovalent ions, and logarithmic relations were found between the electrical conductivity and the contents of divalent ions at the both regions. The relational equations between the electrical conductivity $\lambda_{25}$and the contents of the major inorganic ions at Mulgeum are as follows: $log\;Cl(ppm)\;=\;2.37{\cdot}\lambda_{25}(m{\mho}/cm)+0.733{\pm}0.141$, $log\;SO_4(ppm)=1.12{\cdot}log\lambda_{25}(m{\mho}/cm)+2.14{\pm}0.18$, $log\;Ca(ppm)=0.615{\cdot}log\lambda_{25}(m{\mho}/cm)+1.67{\pm}0.12$, $log\;Mg(ppm)=0.756{\cdot}log\lambda_{25}(m{\mho}/cm)+1.27{\pm}0.11$, $log\;Na(ppm)=2.82{\cdot}\lambda_{25}(m{\mho}/cm)+0.551{\pm}0.133$, $log\;K(ppm)=1.33{\cdot}\lambda_{25}(m{\mho}/cm)+0.136{\pm}0.095$, and total inorganic ions $C(ppm)=399{\cdot}\lambda_{25}(m{\mho}/cm)-0.9{\pm}14.6$. The relational equations between the electrical conductivity ($\lambda_{25}$) and the contents of the major inorganic ions at the region between Namji and Wondong a.e as follows: $log\;Cl(ppm)=4.27{\cdot}\lambda_{25}(m{\mho}/cm)+0.380{\pm}0.138$, $log\;SO_4(ppm)=0.915{\cdot}log\lambda_{25}(m{\mho}/cm)+1.95{\pm}0.18$, $log\;Ca(ppm)=0.756{\cdot}log\lambda_{25}(m{\mho}/cm)+1.74{\pm}0.12$, $log\;Mg(ppm)=1.00{\cdot}log\lambda_{25}(m{\mho}/cm)+1.41{\pm}0.10$. $log\;Na(ppm)=2.47{\cdot}\lambda_{25}(m{\mho}/cm)+0.614{\pm}0.065$, $log\;K(ppm)=1.62{\cdot}\lambda_{25}(m{\mho}/cm)+0.030{\pm}0.060$, and total inorganic ions $C(ppm)=323{\cdot}\lambda_{25}(m{\mho}/cm)+11.7{\pm}9.3$. Logarithmic relations were found between each chemical conservative constituents at Mulgeum and the equations are as follows: $log\;Cl(ppm)=0.711{\cdot}log\;SO_4(ppm)+0.488{\pm}0.206$, $log\;Cl(ppm)=0.337{\cdot}log\;Ca(ppm)+0.822{\pm}0.130$, $log\;Cl(ppm)=0.605{\cdot}log\;Mg(ppm)-0.017{\pm}0.154$, $Cl(ppm)=0.676{\cdot}Na(ppm)+2.31{\pm}4.67$, $log\;Cl(ppm)=0.406{\cdot}log\;K(ppm)-0.092{\pm}0.112$, $log\;SO_4(ppm)=0.378{\cdot}log\;Ca(ppm)+0.721{\pm}0.125$, $log\;SO_4(ppm)=0.462{\cdot}log\;Mg(ppm)+0.107{\pm}0.118$, $log\;SO_4(ppm)=0.592{\cdot}log\;Na(ppm)+0.313{\pm}0.191$, $log\;SO_4(ppm)=0.308{\cdot}log\;K(ppm)-0.019{\pm}0.120$, $Ca(ppm)=0.262{\cdot}Mg(ppm)+0.74{\pm}1.71$. $log\;Ca(ppm)=1.10{\cdot}log\;Na(ppm)-0.243{\pm}0.239$, $Ca(ppm)=0.0737{\cdot}K(ppm)+1.26{\pm}0.73$, $log\;Mg(ppm)=0.0950{\cdot}Na(ppm)+0.587{\pm}0.159$, $log\;Mg(ppm)=0.0518{\cdot}K(ppm)+0.111{\pm}0.102$, and $Na(ppm)=0.0771{\cdot}K(ppm)+1.49{\pm}0.59$. Logarithmic relations were found between each chemical conservative constituents except a relationship between the chloride and calcium contents at the region between Namji and Wondong, and the equations are as follows : $log\;Cl(ppm)=0.312{\cdot}log\;SO_4(ppm)+0.907{\pm}0.210$, $log\;Cl(ppm)=0.458{\cdot}log\;Mg(ppm)+0.135{\pm}0.130$, $Cl(ppm)=0.484{\cdot}logNa(ppm)+0.507{\pm}0.081$, $Cl(ppm)=0.0476{\cdot}K(ppm)+1.41{\pm}0.34$, $log\;SO_4(ppm)=0.886{\cdot}log\;Ca(ppm)+0.046{\pm}0.050$, $log\;SO_4(ppm)=0.422{\cdot}log\;Mg(ppm)+0.139{\pm}0.161$, $log\;SO_4(ppm)=0.374{\cdot}log\;Na(ppm)+0.603{\pm}0.140$, $log\;SO_4(ppm)=0.245{\cdot}log\;K(ppm)+0.023{\pm}0.102$, $log\;Ca(ppm)=0.587{\cdot}log\;Mg(ppm)+0.003{\pm}0.088$, $log\;Ca(ppm)=0.892{\cdot}log\;Na(ppm)+0.028{\pm}0.109$, $log\;Ca(ppm)=0.294{\cdot}log\;K(ppm)-0.001{\pm}0.085$, $log\;Mg(ppm)=0.600{\cdot}log\;Na(ppm)+0.674{\pm}0.120$, $log\;Mg(ppm)=0.440{\cdot}log\;K(ppm)+0.038{\pm}0.081$, and $log\;Na(ppm)=0.522{\cdot}log\;K(ppm)-0.260{\pm}0.072$.