• Animal Bioscience
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• 제34권3_spc호
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• pp.449-456
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• 2021

Objective: Seaweeds could be an alternative and functional feed resource. The purpose of this experiment is to investigate the effect of dietary supplementation of Sargassum meal on laying performance and egg quality of layers. Methods: Two hundred 36-wk-old layers were divided into five treatment groups. Each treatment had four replicates with 10 hens per experimental unit. The corn-soybean meal basal diet was formulated as control group. Sargassum meals were included 0%, 1%, 2%, 3%, or 5% to diets for five treatment groups, respectively. Treatment groups were isocaloric-isonitrogenous diets. Laying performance and egg quality were measured for eight weeks. Results: Sargassum meal supplementation did not affect daily feed intake. Supplementation 1% to 3% of Sargassum meal in diets increased daily laying rate and egg mass compared with those from control group (p<0.05). Egg qualities among five groups were all similar. Supplementation of 3% Sargassum meal increased the lightness of egg yolk (p<0.05). Eggs produced from layers fed 1% and 2% Sargassum meal had a higher consumer's acceptability than the control group (p<0.05). In blood characteristics, contents of glucose, nitrogen, triiodothyronine (T3) and thyroxine (T4) increased as the increase of supplementation ratio of Sargassum meal (p<0.05). In serum antibody titers, supplementation of 2% Sargassum meal stimulated a higher immunoglobulin M (IgM) level than that from control group (p<0.05). However, IgM content of layers fed diets with Sargassum meal ≥3% were decreased (p<0.05). There was no difference in IgA and IgG titers among groups. Conclusion: Supplementation of 1% to 3% Sargassum meal has shown to increase egg laying rate and egg mass of Leghorn layers. However, high supplementation (5%) would negatively affect laying performance. In consideration of laying performance, egg quality, consumer responses, and blood antibody, supplementation of Sargassum meal was suggested 2% in the diet for layers.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1460-1464
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• 2001

This study compared the lipid metabolism of white Leghorn layers between the growing and the laying periods. The study was conducted on thirty layers in the growing period (14 weeks old) and in the initial laying period (36 weeks old; 14 weeks from the onset of laying). Results indicated that all plasma lipid traits were significantly different (p<0.05) between the two periods. The estrogen concentration in the laying period was about 3 fold that in the growing period. Triacylglycerol (TG) concentration in the laying period was about 12 fold that in the growing period. The phospholipids, cholesterol, glycerol and non esterified fatty acid (NEFA) in the laying period were also higher than those in the growing period (p<0.05). Lipogenic enzyme activities in the laying period were higher than in the growing period (p<0.05). High-density lipoprotein (HDL) was the largest lipoprotein portion in growing layers. In laying hens, the major lipoprotein portion was very low-density lipoprotein (VLDL). It was also shown that protein is the primary component of HDL and TG is the largest portion of VLDL in both periods. In laying hens, VLDL contained more TG than that in growing layers (p<0.05). The VLDL particle size in laying hens was larger than that in growing layers (p<0.05). This study indicates that the lipid metabolism traits of a layer in the laying period were different from those in the growing period. The lipogenesis related traits in the laying period were markedly greater than in the growing period.

• 한국가금학회지
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• 제16권2호
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• pp.73-81
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• 1989

본 연구는 산란계의 개량을위한 주요 경제형질에 대한 유전력과 유전상관을 추정하고자 1980년 3월 1일부터 1981년 7월 31일까지 사육 되어온 S.C.W. Leghorn종 351수와 R. 1. Red종 326수에서 조사된 주요 경제형질에 대한 자료로서 얻어진 결과들을 요약하면 다음과 같다. 1. 체중은 S.C.W. Leghorn종과 R. I. Red종에서 각각 초산 시 1,409, 1,965 g이었고 300일영기 1,602, 2,305g이었으며, 500일영시 1,709, 2,479 g이었고, 초산일영은 S.C.W. Leghorn종과 R. I. Red종에서 각각 156, 163일이었다. 또한 산란수는 S.C.W,Leghorn종과 R. I. Red종에서 300일영시에 각각 101.18, 101. 05개이었고. 500 일영시에 각각 214.39 및 214.93개이었으며, 난중은 초산시에 41.93, 41.82g, 300일영시에 57.65, 57.62g, 500일영시에 60.33, 60.57g이었다. 2. 유전력 추정치에 있어서는 S.C.W. Leghorn종 및 R. I. Red종의 체중은 부분산성분에서 각각 0.492∼0.612, 0.309∼0.523이었고, 최분산성분에서 각각 0.275∼0.458, 0.134∼0.380으로 부분산성분에서 높았으며, 초산일영은 부분산성분에서 각각 0.167, 0.169이었고, 모분산성분에서 각각 0.169, 0.095로 낮은 추정치이었으며, 산란수는 부분산성분에서 각각 0.214∼0.239, 0.137∼0.259이었고, 모분산성분에서 각각 0.336∼0.341, 0.024∼ 0.102 이었으며, 난중은 부분산성분에서 각각 0.537∼0.796, 0.519∼0.631이었고, 모분산성분에서 각각 0.374∼0.686 및 0.116∼0.365로 부분산성분에서 높게 추정되었다. 3. 유전상관에서 S.C.W. Leghorn종과 R. I. Red종에서 체중과 초산일영간에는 각각 0.328∼0.426, 0.186∼0.244이었고, 체중과 산란수간에는-0.666∼-0.498, -0.542∼-0.073으로 員의 상관인 반면에, 난중과는 각각 0.384∼0.744, 0.126∼0.612로 정의 계수이었고, 초산일영과 산란수간에는 각각 -0.639∼-0.452, -0.754∼-0.320으로 높은 부의 상관인 반면에 난중과는 각각 0.478∼0.705, 0.021∼0.605로 정의 상관이었고, 산란수와 난중간에는 각각 -0.623∼-0.355, -0.861∼-0.327로 부의 상관계수이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.948-953
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• 2007

The relative performance and immune response was evaluated in White Leghorn layers fed liquid DL-methionine hydroxyl analogue-free acid (MHA-FA) relative to dry DL-methionine (DLM) in maize-soybean-sunflower based diets. Three graded levels of methionine (Met) from DLM or MHA-FA were added to the basal diet containing 0.27% Met on an equimolar basis to achieve 0.30, 0.36 and 0.42% Met in the diet. Each diet was fed ad libitum to 25 replicates of one bird (individual feeding) each, from 24 to 40 weeks of age. A regime of 16 h light was provided and all the layers were kept under uniform management throughout the experimental period. None of the parameters studied were influenced by the interaction between source and level of Met in diets. Similarly, the majority of parameters, except for daily feed consumption and immune response (influenced by level) and egg specific gravity and shell thickness (influenced by source), were not affected by either source or level of Met in the diets. Feed consumption was significantly lower in the birds fed a diet containing 0.42% Met compared to those fed lower levels of Met. The cutaneous basophilic hypersensitivity response to PHA-P and antibody titre (32 and 40 wk) to inoculation of sheep red blood cells increased significantly by increasing the concentration of Met in the diet from 0.30 to 0.36%. Thus, the Met requirement for immune competence was higher than for optimum production. The source of Met significantly influenced the egg specific gravity and shell thickness. The specific gravity and shell thickness of eggs increased significantly when MHA-FA was used as the source of Met in the diet compared to DLM. From the study it is concluded that Met requirement for immune competence (360 mg/b/d) is higher than for optimum production (300 mg/b/d). MHA-FA was comparable with DLM as a source of Met for production performance and immunity, when the bioavailability of MHA-FA was considered as 88% of DLM. Further, MHA-FA improved egg shell quality compared to DLM.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1171-1175
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• 2005

An experiment was conducted (28 to 44 weeks) to study the laying performance, shell quality, and nutrient retention of White Leghorn layers fed different levels of non-phytate phosphorus (NPP). Six levels of NPP (0.15, 0.18, 0.21, 0.24, 0.27 and 0.30%) at a constant calcium (Ca) level (3.5%) in maize-soya-deoiled rice bran based diets were formulated, and each experimental diet was offered ad libitum for 16 weeks to five replicates with five birds in each replicate. The body weight of WL layers fed diet containing 0.15% NPP was significantly (p<0.05) lower than those fed diet with 0.30% NPP, at 44 weeks of age. However, the hen day egg production, egg weight, daily feed intake and feed consumed per dozen eggs were not influenced by the variation in the NPP levels in the diet. The bone ash content was significantly (p<0.05) higher in the birds fed 0.30% NPP as compared with those fed diets up to 0.24% NPP. Bone ash content was intermediate in the birds fed diet containing 0.27% NPP. The tibia strength followed the same trend as that of bone ash. Dietary NPP content had no influence on serum Ca and protein concentration and activity of alkaline phosphatase. However, serum inorganic P concentration increased linearly with NPP content in the diet. The concentration of P was significantly (p<0.05) higher in the birds fed 0.27% NPP or higher as compared with those fed 0.15% NPP. Levels of dietary NPP had no influence on egg quality parameters like shell wt, shell thickness, shell strength and specific gravity. The retention of nutrients such as DM, N and Ca were comparable among the WL layers fed different levels of NPP. However, the retention of P decreased linearly with increase in the level of NPP in the diet. The retention of P in the birds fed diets up to 0.24% NPP in the diet was comparable, however further increasing the content of NPP (either 0.27% or 0.30%) reduced the retention of P. Based on the results of the present study, 0.15% NPP (180 mg/b/d) in the diets of WL layers is adequate for optimum production performance during 28 to 44 weeks of age, however, WL layers require 0.27% NPP (324 mg /b/d) in the diet for optimum production with better bone mineralization.

• Animal Bioscience
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• 제36권4호
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• pp.609-618
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• 2023

Objective: TLRI 211-1 is a novel Bacillus subtilis strain. This experiment was to investigate dietary supplementation of TLRI 211-1 on laying performance, egg quality and blood characteristics of layers. Methods: One hundred and twenty 65-wk-old Leghorn layers were divided into four treatment groups for 8 weeks experiment. Each treatment had three replicates. The basal diet was formulated as control group with crude protein 17% and metabolizable energy 2,850 kcal/kg and supplemented with TLRI 211-1 0.1%, 0.3%, and commercial Bacillus amyloliquefaciens 0.1% as treatment 2, 3 and 4 groups, respectively. Both TLRI 211-1 and commercial Bacillus amyloliquefaciens were adjusted to contain 1×10⁹ colony-forming unit (CFU)/mL (g), hence the 0.1% supplemental level was 1×10⁹ CFU/kg. Results: The results showed that TLRI 211-1 0.3% and commercial B. amyloliquefaciens groups had higher weight gain than the other groups; TLRI 211-1 0.1% group had better feed to eggs conversion ratio than the control and commercial B. amyloliquefaciens groups (p<0.05). Bacillus subtilis supplementation increased yolk weight (p<0.05). In egg quality during storage, TLRI 211-1 0.1% had higher breaking strength than the control group at the second week of storage (p<0.05). At the third week of storage, TLRI 211-1 0.3% had higher Haugh unit (p<0.05). Hens fed diets supplemented with TLRI 211-1 0.3% significantly decreased blood triglyceride levels and increased blood calcium levels (p<0.05). TLRI 211-1 0.3% group had lower H₂S (p<0.05) and hence had less unpleasant odor in excreta of hens. Conclusion: In conclusion, supplementation with 0.1% TLRI 211-1 can significantly improve feed to eggs conversion ratio. TLRI 211-1 supplementation also can maintain eggs at their optimum quality level during storage. The study showed that B. subtilis TLRI 211-1 can be used as feed additives for improving egg production performance and egg quality.

• 한국가금학회지
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• 제16권2호
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• pp.83-89
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• 1989

본 연구는 산란계의 개량을 위한 난구성분의 유전역과 유전상관을 추정하고자 1980년 3월 1 일부터 1981년 7월 31일까지 사육 되어온 S.C.W. Leghorn종 351수와 R. I. Red종 326鳶수에서 생산된 계란 6,093개의 난구성분을 조사 분석하여 얻어진 결과를 요약하면 다음과 같다. 1. 평균난백중은 S.C.W. Leghorn종과 R. I. Red종에서 각각 초산시에 28.67, 28.95g, 300일영에 36.28, 36.015, 5일영에 37.15, 36.855이었고, 난황중은 각각 초산시에 9.21, 9.465, 300일영에 15.95, 16.435, 500일영에 17.86, 18.54 g 이었으며, 난각중은 각각 초산시 4.04, 3.66 g, 300일영에 5.39, 5.13g, 500일영에 5.40, 5.28 g이었다. 2. 유전력 추정치에 있어서는 S.C.W. Legrn종 및 R. I. Red종의 난백중은 부분산성분에서 각각 0.637∼0.746, 0.94∼0.563 이었고, 모분산성분에서 각각 0.412∼0.496, 0.477∼0.620으로 대체로 높은 추정치이었으며, 난황중은 부분산성분에서 각각 0.213∼0.530. 0.253∼0.437이었코, 모분산성분에서 각각 0.343∼0.613, 0.389∼0.477로 모분산성분에서 높았으며, 난각중은 부분산성분에서 각각 0.427∼0.602, 0.141 ∼0.281 이었고, 모분산성분에서 각각 0.336∼0.409 및 0.380∼0.552이었다. 3. 난구성분들간의 유전상관은 난백중과 난황중간에는 S.C.W, Leghorn종과 R. I. Red종에서 각각 0.082∼0.399 및 0.142∼0.465로 저도 또는 중도의 상관인 반면에 난백중과 난각중간에는 각각 0.674∼0.952 및 0.216∼0.546으로 높거나 중정도의 정의 상관이었고, 난황중과 난각중간에는 각각 0.273∼0.771 및 0.122∼0.749이었다.각 0.47~0.52, 0.40~0.54로 유전력이 비교적 높은 형질임을 알 수 있다. 그러나 총산란수(TEN)와 총산란중량(TEM)의 유전역은 각각 0.07~0.37, 0.18~0.27로 유전력이 낮은 형질임을 나타내고 있으며 모든 산란형질의 유전력이 모분산성분에 의한 추정치가 부분산성분에 의한 추정치 보다 높게 나타나서 이들 형질의 모체효과를 포함한 비상가적 유전분산의 효과를 시사하고 있다. 3. 퇴교배에 따른 유전력 변화를 부모분산성분에 의하여 살피보면 기초계군(BC0), 퇴교배 1세대(BC1), 퇴교배 2세대(BC2)로 퇴교배가 증가함에 따라 초산일령(SM)은 0.47, 0.42, 0.51 이였으며 총산란수(TEN)에서는 0.28, 0.13, 0.27으로 유전역 변화의 일정한 경향치를 보이지 않았다. 그러나 평균난중(AEW)과 총산란 중량(TEM)에서는 기초계군(BC0), 퇴교배1세대(BC1), 퇴교배2세대(BC2)로 퇴교배가 증가함에 따라 0.59, 0.43, 0.35와 0.28, 0.20, 0.18로 추정되어 뚜렷한 유전력의 감소를 보이고 있다. 이것은 퇴교배가 증가함에 따라 평균난중과 총산란중량에 대한 유전적 변이의 감소에 기인된 것으로 생각된다. 4. 산란형질간의 유전상관을 살펴보면 초산일령(SM)과 총산란수(TEN)간의 유전상관은 -0.55이고 초산일령(SM)과 총산란중량(TEM)간은 -0.42로 부의 상관을 보이고 있다. 그러나 초산일령(SM)과 평균난중(AEW)간은 0.20으로 낮은 정의 상관을 나타내고 있다. 평균난중(AEW)과 총산란수(TEN)간은 -0.29이고 평균난중(AEW)과 총산란중량(TEM)간은 0.31의 낮은 유전상관을 보이고 있다. 한편 총산란중량(TEM)과 총산란수(TEN)간은 0.82의 높은 정의 상관을 나타내므로 이상의 결과에서 총산란중량(TEM)에 관여하는 것은 평균난중(AEW) 보다는 주로 총산란수(TEN)에 기인하는 것 같다. 또한 총산란수( TEN)는 초산일령(

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.290-296
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• 2003

Tsaiya ducks and Leghorn hens are the two major laying birds raised in Taiwan. They are all excellent egg layers. Tsaiya ducks are small in body size (1.3 kg) with bigger egg weight (65 g) and stronger eggshell breaking strength than eggs from hens. The eggshell consists mainly of calcium carbonate, hence calcium plays an important role in the eggshell formation. Magnesium is also present in eggshell in small amounts, which may have effect on maintaining eggshell quality. In comparison studies, it was shown that the duck eggshells contained higher calcium and lower magnesium content than chicken eggshells. The eggshell magnesium content was not affected by the dietary magnesium levels (690-2380 ppm) in ducks, but in hens, it increased linearly with dietary magnesium levels. The palisade layer ($5000{\times}$) of the eggshell was found to have a compact form for ducks while there are many hallow vesicles in chicken eggshells. The eggshell magnesium deposition model is different for ducks and hens with ducks having a one-peak and hens having a two-peak model. The calcium deposition model is similar for both birds. Both the carbonic anhydrase specific activity and total activity in the shell gland mucosa of ducks are higher than those in hens. Ducks retain higher magnesium and lower calcium in the shell gland mucosa and secret less magnesium and more calcium into the shell gland lumen for eggshell deposition. The ATPase specific activity is maintained fairly constant during the eggshell forming stage, indicating continuous calcium transport into the shell gland lumen for eggshell formation. The magnesium content in duck eggshells is much lower than that in hens indicating that the magnesium content in the eggshell may have an effect on eggshell quality.

• Journal of Biosystems Engineering
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• 제31권5호
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• pp.436-442
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• 2006

This research was conducted to design and develop a wired monitoring system for judging if sick or dead layers (SDL) exist in multi-tier layers battery (MLB) by machine vision, and to analyze its performance. In this study, 20 Brown Leghorn (Hi-Brown) layers aged 37 weeks old, were used as the experimental animals. The intensity of concern paid by layers on feed was over 90% during 5 minutes and 30 seconds after providing feed, and normal layers (NL) had been standing to take feed for that period. Therefore, in this study, the optimal judging time was set by this test result. The wired monitoring system developed was consisted of a driving device for carrying machine vision systems, a control program, a RS232 to RS485 convertor, an automatic positioning system, and an image capture system. An image processing algorithm was developed to find SDL in MLB by the processes of binary processing, erosion, expansion, labeling, and reckoning central coordinate of the captured images. The optimal velocity for driving unit was set up as 0.13 m/s by the test results for wired monitoring system, and the proximity switch was controlled not to be operated for 1.0 second after first image captured. The wired monitoring system developed was tested to evaluate the remote monitoring performance at lab-scale laying hen house. Results showed that its judgement success.ate on normal cage (without SDL) was 87% and that on abnormal cage (with SDL) was 90%, respectively. Therefore, it would be concluded that the wired monitoring system developed in this study was well suited to the purpose of this study.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1638-1642
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• 2006

An experiment was conducted to elucidate the effect of graded levels of vitamin $D_3$ in White Leghorn (WL) layer breeders on egg production, shell quality, hatchability of eggs and juvenile performance of offspring during their late laying period (72-88 wk). White Leghorn breeder females were randomly divided into 5 groups of 50 each and were housed in individual California cages in an open-side housing system. Considering birds in five cages as a replicate, 10 such replicates were randomly allotted to each treatment. A basal diet was formulated containing all the nutrients as recommended for WL layers except vitamin $D_3$, which served as control. Another, four diets were formulated by supplementing graded levels of feed grade crystalline cholecalciferol to the basal diet that contained 300, 600, 1,200 and 2,400 ICU of vitamin $D_3$ per kg. Each diet was offered ad libitum to one of the above five treatment groups. The egg production, egg weight, daily feed consumption and the feed intake per dozen eggs or kg egg mass of the birds fed diet without any supplemental vitamin $D_3$ was comparable with those of supplemental groups. Similarly, the level of vitamin in the diet did not have any effect on any of the above parameters. However, the specific gravity of eggs laid by the birds fed the diet without supplemental vitamin $D_3$ was comparable with either 600 or 2,400 ICU supplemental groups but significantly higher when compared to the 300 and 1,200 ICU groups. The egg -shell breaking strength was significantly lowered in the 600 ICU supplemental groups as compared to the strength of other dietary groups. The Haugh unit, egg shell weight, shell thickness, tibia breaking strength, bone ash and calcium content were not influenced by vitamin $D_3$ concentration in the diet. Serum Ca concentration was influenced by vitamin $D_3$ level in the diet. The serum Ca concentration of birds fed either control or the vitamin supplemented diet up to 1200 ICU/kg diet was comparable. However, increasing the concentration of vitamin $D_3$ to 2,400 ICU/kg diet significantly enhanced the concentration of Ca in the serum, which was significantly higher compared to other dietary groups. The serum concentration of P and protein, however, was not influenced by level of vitamin $D_3$ in the diet. Neither fertility nor hatchability was influenced by vitamin $D_3$ concentration in the diet. Feeding a vitamin $D_3$ deficient diet or supplementation of vitamin to hens did not have any influence on their progeny chicks. It can be concluded that dietary supplementation of vitamin $D_3$ may not be essential for optimum production, shell quality, hatchability, and juvenile performance of WL breeders during 72 to 88 weeks of age.