• Parasites, Hosts and Diseases
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• v.59 no.3
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• pp.303-309
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• 2021

In this study, we intended to describe an unrecorded species of heterophyid trematode recovered from the small intestine of a Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 13 small flukes were collected from a deceased Korean raccoon dog which was found in Chuncheon-si, Gangwon-do, Korea in May 2017. The trematode body were covered with many small spines, rectangular, broader than long, 807-1,103 ㎛ long and 1,270-1,550 ㎛ wide. Oral sucker in the anterior end slightly smaller than acetabulum. Pharynx muscular and well developed. Esophagus relatively long and sigmoid. Acetabulum small and located at median in anterior 2/5 portion. Ceca bifurcated at the anterior of genital pore and acetabulum and terminated at testis level. Testes larger, deeply lobed and located at the near of posterior end of body. Ovary small, triangular and located at the slight left of median and the anterior of left testis. Vitelline follicles dendritic and extend from the middle level of esophagus to the posterior portion of body. Eggs embryonated, operculated, small and 33-35×15-16 ㎛ in size. Based on the morphological characteristics, the small heterophyid flukes recovered from the small intestines of Korean raccoon dog, N. procyonoides koreensis, were identified as Euryhelmis squamula (Digenea: Heterophyidae). Accordingly, this species of heterophyid flukes is to be a new trematode fauna in Korea by this study.

• Journal of Genetic Medicine
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• v.18 no.2
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• pp.142-146
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• 2021

Alagille syndrome (AGS) is a rare autosomal dominant inherited disorder, with major clinical manifestations of bile duct paucity, cholestasis, cardiovascular anomaly, ophthalmic abnormalities, butterfly vertebrae, and dysmorphic facial appearance. It is caused by heterozygous mutations in JAG1 or NOTCH of the Notch signaling pathway presenting with variable phenotypic penetrance and involving multiple organ systems. The following case report describes a unique case of a 16-year-old female with AGS who presented with the primary complaint of renovascular hypertension. She had a medical history of ventricular septal defect and polycystic ovary syndrome. The patient had a dysmorphic facial appearance including frontal bossing, bulbous tip of the nose, a pointed chin with prognathism, and deeply set eyes with mild hypertelorism. Stenoocclusive changes of both renal arteries, celiac artery, lower part of the abdominal aorta, and left intracranial artery, along with absence of the left internal carotid artery were found on examination. Whole exome sequencing was performed and revealed a pathologic mutation of JAG1, leading to the diagnosis of AGS. Reverse phenotyping detected butterfly vertebrae and normal structure and function of the liver and gallbladder. While the representative symptom of AGS in most scenarios is a hepatic problem, in this case, the presenting clinical features were the vascular anomalies. Clinical manifestations of AGS are diverse, and this case demonstrates that renovascular hypertension might be in some cases a presenting symptom of AGS.

• Journal of Embryo Transfer
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• v.1 no.1
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• pp.76-80
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• 1986

This study was carried out to investigate the inte actions between recipients and embryos to compare pregnancy rates in bovine embryo transfer, such as synchrony and stage of embryos, synchrony and quality of embryos, synchrony and side of uterine horn, and preservation time and stage of embryos. Fifty-two embryos were transferred by surgically to 42 Holstein heifers, 3 Holstein cows and 7 Korean native heifers from Feb., 1985 to June, 1986. The results were as follows: 1. In the trial of interactions between synchrony and embryo stage, recipients synchronired from- hours to + 12 hours in synchrony and embryos from morulac stage to advanced blastoctyst stages showed reason able pregnancy rate. 2. Excellant (A) and good (B) grade embryos showed good pregnancy rate, 81.8% and 73.3% respectively, but fair (C) grade embryos showed poor, 25% only at the same boundary of recipient synchrony. 3. More recipients had corpus luteum on the right ovary than the left, and also had better pregnancy rate (26/32 vs 13/20,81.3% vs 65%). 4. A good pregnancy rates (over 60%) were obtained with the embryo transferred 17hrs after flush at room temperature or field condition in culture medium.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.225-232
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• 1996

The purpose of this study was to evaluate effects of hormonal treatments on corpus lutea, follicles and development stage of embryos for enhancing the production efficiency of in vivo porcine embryos suitable to introduce fo foreign genes. Hundred and twenty gilts were allocated to 6 experimental group in different combinations of hormones PG 600, PMSG, hCG and altrenogest. When gilts were treated with chorionic gonadotrophin 200 IU and serum gonadotrophin 200 IU(PG 600), altrenogest, serum gonadotrophin (PMSG) 1,000 IU, and chorionic gonadotrophin(hCG) 750 IU (PAPh), the numbers of corpus luteum (30.4) were significantly higher than those of other treatment groups (P<0.05). The number of corpus luteum from ovary in either right (9.1) or left (10.1) side was not significantly changed with hormone treatments. Number of follicles in control was 20.7, which was higher than those of hormonal treatment groups. The average numbers of 1, 2, 4 and 8 cell staged embryos were 8.1, 1.4, 1.6 and 1.0 in control, but the numbers of 1-cell stage in PAPh treatment group was 24.2, which was significantly higher than those of treatment groups (P<0.05). Therefore, these data indicated that hormonal treatment, especially PAPh, enhanced the developments of follicles, corpus lutea and embryos and increased the collection rate of the 1-cell stage embryos to introduce of foreign genes.

• Development and Reproduction
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• v.5 no.2
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• pp.137-144
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• 2001

Gametogenesis and gonadal development of the sea squirt Halocythia roretzi, which is two years old were investigated by histological study. The specimens were collected in Guryong-po coastal area Kyoungsangbuk-do, Korea from May 1996 to April 1997. The sea squirt is hermaphrodite and oviparous. The ovary is located in the inner wall of the tunic year-round, but the testis can be distinguished from in June. The ovary is composed of 6∼8 gonoducts at the left side and 8∼10 ones at the right side, the testis consists of the complex gonad having irregular sacular structures. Oogonia in the ovarian sac were 11.7∼15.6 ${\mu}m$ in diameter. The early developing oocytes were 39.6∼47.6 ${\mu}m$ and nucleus 10.0∼25.0 ${\mu}m$ in diameter. Oocytes in the ovarian sacs during vitellogenesis were 158.6∼210.0 ${\mu}m$, and fully ripe oocytes which were to 210.0∼230.9 ${\mu}m$ in diameter had several test cells in the cortical parts showing a characteristic of vertebrate. The testis showed a general spermatogenesis as in the marine animals. The three-year old sea squirt occurred the first spawning between January to February under 10$^{\circ}C$

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.4
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• pp.236-246
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• 2023

Background: This study helps to evaluate the Ovarian potential of Cameroonian Zebu cattle (Bos indicus) slaughtered in Etoudi - Yaoundé for implementing Assisted Reproductive Techniques (ARTs). The aim was to enhance the productivity of the cattle sub-sector in Cameroon while conserving local genetic resources. Methods: A total of 144 cows, including 94 cycled cows and 50 pregnant cows, were included in the study. Live weights were determined by measuring the thoracic perimeter of each cow using a Rondo measuring tape. Age was determined postmortem through examination of dentition and horns, while the uterus and ovaries were removed, weighed, and classified based on physiological status (pregnant or nonpregnant). Follicles were counted, and their diameters were measured and categorized into small (Ø < 3 mm), medium (Ø 3-8 mm), and large (Ø > 8 mm). Results: The results revealed an average follicular population of 32.02 ± 9.31 per cow, with 22.43 ± 8.45 small follicles, 8.42 ± 3.87 medium follicles, and 0.76 ± 0.34 large follicles. The weight of the right ovary was significantly higher than that of the left ovary (p < 0.05), and cows aged 6 to 9 years exhibited a higher number of follicles compared to other age groups. Cows with medium (BCS = 3) and large (BCS = 4-5) body reserves had the heaviest ovaries. Additionally, pregnant cows had heavier uteri compared to non-pregnant cows, and cows with a body condition score of 3 or higher had higher uterine weights than lean cows (BCS = 1-2). Conclusions: This study demonstrates that age, body condition score, and pregnancy status influence ovarian weight. Body Condition Score serves as a reliable indicator of cow nutritional status, and cows with BCS of 3-5 are excellent candidates for in vitro production of embryos.

• Advances in pediatric surgery
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• v.1 no.2
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• pp.107-114
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• 1995

Three hundred and twenty-seven patients of 2,046 inguinal hernia cases Primarily repaired at Hanyang University Hospital had the history of incarceration or presented as incarcerated inguinal hernia on admission. Incidence of incarceration of all male hernias was 14.2%(234 patients) and 22.7%(93 patients) of all female cases. Incarceration occurred in 17.3% of all right hernia cases and in 13.7% of all left hernia cases. The incarceration occurred 52.6% of the hernia patients in the first month of life, 27.3% in the first year, 26.7% in the second year and 7.8% after 2 years of age. Strangulated inguinal hernia occurred in 8 patients: five patients had ovaries involved, two patients intestines, and one patient omentum. Emergency operations were performed on 66 patients(20.2%) because incarcerated hernia could not be reduced by taxis. At the time of operation, the hernia sacs were empty in 140 of 327 patients and the remainders contained omentum(50), small intestine (44), appendix and/or cecum(28), sigmoid colon(2), ovary and/or tube(66), and omental cyst(1). An elective hernia repair should be performed promptly after presentation of the hernia, especially before 2 years of life because of high incidence of incarceration. In this study, of 327 incarcerated hernia, 187 patients(57.2%) did not have prior history of incarceration and incarceration developed more than 7 days after hernia onset in 95.6%. If the hernia repairs had been performed within 7 days after hernia onset, about half of the incarceration might have been prevented.

• Journal of Embryo Transfer
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• v.17 no.1
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• pp.23-32
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• 2002

To establish an effective in vivo Hanwoo embryo production system, several factors including types of FSH analogue, FSH treatment day, grade of Corpus Luteum were examined. Among FSH analogues. Super-OV was shown to have lower effect on transferable embryo production (P<0.01). Regarding the day of FSH treatment, there were significant difference in estrus status of donors(P<0.1), fertilization rate, and transferable and freezable embryo production(P<0.05). Finally, embryo production was significantly(P<0.01) higher in grade A (8.06 ∼9.49) Corpus Luteum which showed more than 6 on the left or right ovary than in grade B, C and D(2.93∼6.28).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1351-1355
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• 2014

Background: Ovarian sex-cord stromal tumours (SCST) are rare, and relatively infrequent in children. These have to be distinguished from more common germ cell tumors in children and also from benign epithelial neoplasms. Objectives: The purpose of our study was to report the clinical and pathological findings in young patients with these tumours in our population. Material and Methods: The present observational cross-sectional study included all subjects <21 years of age diagnosed with ovarian SCST, in Aga Khan University Hospital Histopathology Laboratory, Karachi, Pakistan, from January 1992 till July 2013. Results: Of the total of 513 SCSTs presented during the study period, 39 fulfilled inclusion criteria and were assessed. The age range was 4-250 months. Most of the tumours presented at stage-1 and an abdominal mass was the most common presenting symptom, along with menstrual disturbance. The left side ovary was slightly more affected (53.5%). Of the total, 15 were juvenile granulosa cell tumours (JGCT), 11 sclerosing stromal tumours (SST), 10 of the fibrothecomas spectrum, 2 Sertoli leydig cell tumours (SLCT) and one a sex cord tumour with annular tubules (SCTAT). Detailed immunohistochemical analyses were performed in 33 cases. Recurrence/metastasis was noted in 4/21 cases with follow-up data. Conclusions: Ovarian sex cord stromal tumours are very rare in young age in our population, and usually present at an early stage. Most common among these are juvenile granulosa cell tumours, although surprisingly sclerosing stromal tumours were also common. Clinical symptoms due to hormone secretion in premenstrual girls and menstrual disturbance in menstruating girls are common presenting features.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.428-430
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• 2005

A female French Bulldog was referred to Veterinary Medical Teaching Hospital, Chungbuk National University. In case, alopecia and erythema nodosum were the main complaints in a 16 months old female dog. Ventral alopecia and dorsal erythema nodosum have been presented on skin lesions for 8 months. There were no specific change with CBC and blood chemistry but plasma estrogen concentration was 68 pg/ml and cornified superficial epithelial cells were detected above $90\%$ by cytology examination of vaginal smear preparation. Ovarian cyst was detected by ultrasonography in the left ovary. Cystic diameter was $14.2\times12.0mm$. Therefore, we diagnosed as unilateral multiple follicular cysts. The dog was treated with GnRH $50{\mu}g$ injection by intramuscularly Cyst size was decreased $7.3\times7.2mm$ after 7 days GnRH treatment and disappeared after 14 days GnRH treatment. Also dermatitis by unilateral multiple follicular cysts were recovered after 21 days GnRH treatment.