• Title/Summary/Keyword: LeeWhang

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Analysis of Vaginal Lactic Acid Producing Bacteria in Healthy Women

  • Nam, Hye-Ran;Whang, Kyung-Hee;Lee, Yeon-Hee
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.515-520
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    • 2007

  • Vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on Blood and DeMan-Rogosa-Sharpe agar, PCR with group-specific primers for Lactobacillus-denaturing gradient gel electrophoresis (DGGE), and PCR with specific primers for V3 region in 16S rRNA-temporal temperature gel electrophoresis (TTGE). Conventional isolation method on media detected only one lactobacillus (Lactobacillus brevis) while TTGE detected only Lactobacillus sp. DGGE detected seven Lactobacillus species; L. coleohominis, L. crispatus, L. iners, L. reuteri, L. rhamnosus, L. vaginalis, and Leuconostoc lactis. L. acidophilus and L. gasseri, which are prevalent in Western women, were not detected in Korean women. Furthermore, L. rhamnosus, Leuc. lactis, L. coleohominis, and Weissella cibaria, which were not previously reported in the vaginal microbiota of Korean women, were detected. The five most prevalent LABs in vaginal microbiota in Korean women were L. iners, Enterococcus faecalis, L. crispatus, Leuc. lactis, and W. cibaria.

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Stability of Cinnamadehyde and Cinnamic acid of Cinnamomi Cortex Preparations (계피함유 제제의 Cinnamaldehyde 및 Cinnamic acid의 안정성)

  • Lee, Yu-Jin;Park, Hee-Juhn;Park, Jong-Hee
    • Korean Journal of Pharmacognosy
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    • v.37 no.1 s.144
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    • pp.53-55
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    • 2006

  • Using HPLC, we examined quantitative change of cinnamaldehyde and cinnamic acid, caused by time-progress and temperature-change for the Cinnamoni Cortex preparations, Gye Ji Ryung Whan(桂枝茯?丸), Pal Mi Ji Whang Whan(八味地黃丸) and Do Haig Sung Ki Tang(桃核承氣湯). The content of cinnamaldehyde significantly decreased by the increase of temperature. It is suggested that cinnamaldehyde and cinnamic acid are considerably stable when kept frozen.

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