• 한국동물위생학회지
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• 제29권2호
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• pp.97-102
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• 2006

A total of 1,848 samples was taken from bovine and porcine from January 2003 to November 2005. They were examined for the presence of Clostridiuml perfringens. The properties of the isolates were characterized for Gram staining, biochemical features and enterotoxin production. Forty-one strains (2.2%) of C perfringens were isolated from the 1,848 bovine and porcine carcass using selective media. 30 (3.2%) C perfringens were isolated from the 925 of bovine cacasses, and 11(1.2%) were isolated from the 923 of porcine cacasses. In TSC agar, all isolates showed lecithinase activity. The isolation rate was higher in spring and summer than in autumn and winter. Among 41 isolates, only 1 isolate detected the cpe gene in PCR. The PCR amplified band was observed 233 bp.

• 식물병과 농업
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• 제5권1호
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• pp.1-7
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• 1999

The grain rot of caused by Bukholderia glumae was fist reported in japan in 1955 and then reported in other countries as well as in Korea in 1986. The pathogen causes both seedling and grain rot of rice but it cannot attack any other parts of adult rice plant. Bacterial colonies grow slowly, and are circular and greyish white. The causal bacterium is Gram-negative and rod shape with 1-3 polar flagella, and produce a diffusible yellow-greenish nonfluorescent pigment on King's medium B. Biochemical characteristics such as negative in arginine dehydrolase, oxidase reaction and nitrate reduction and positive in lecithinase, and the utilization of L-arginine and inositol are useful in differentiation of this from other nonfluorescent bacteria pathogenic to rice. This pathogenic bacterium had belonged to the genus of Pseudomonas but recently was transferred to the new genus Burkholderia on the basis of physiological characteristics and DNA-DNA hybridization data. However, other characteristics such as colony heterogenicity or colonial variation after subcultures, phytotoxin, secreting antibiotics, and relationship between yellow greenish pigment production and pathogenicity need to be clarified more. To develop an effective control strategy for this disease, understanding of detailed life cycle of the disease and critical environmental factors affecting disease development is prerequisite. Although 5,435 ha of rice paddy in Korea was infested during 1998, there is no exact estimation of yield losses and distribution of the pathogen. The review will focus on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

• Journal of Microbiology
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• 제39권4호
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• pp.321-325
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• 2001

Baird-Parker agar with egg yolk/tellurite emulsion (BPA) is widely accepted as a medium for the enu-meration of Staphylococcus aureus in foods. Howerver, it is not vompletely selective and colonies of other genera of species could be similar to those of Staphylococcus aureus. Moreover, the strains of Staphylococcus aureus that are lecithinase negatrive could go unnoticed. Both facts could affect the counts. The aim of this study was to dtermine whether the enumeration of the colonies with the typical morphol-ogy of Staphylococcus aureus on BPA is sufficien to quantity this species in poultry meat. Forty chiken carcasses were tested for Staphylococcus aureus by surface plating using BPA, Results indicate that the predictiv value of the morphoogy of the colonies of BPA is 85.71% and 68.42% for typical and atypical colonies of Staphylococcus aureus, respectively. However, Staphylococcus aureus counts (after identification) and counts of typical colonies did not show any significant differences(P>0.05) and are significantly(P<0.001) correlated (r=0.996).These results suggest that , for screening purposes. enumeration of Staphylococcus aureus from poultry meat does not require any identification of stains. resulting in a saving of time and money.

• 한국토양비료학회지
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• 제34권6호
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• pp.394-400
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• 2001

가축분 및 음식물 퇴비 132점에서 517균주를 분리하고 CMC와 xylan 및 PGA 배지에서 강한 활성을 가지는 10여개의 균주를 선별하여 배추종자 발아를 이용한 작물 inhibition test와 오이 육묘실험으로 growth test를 거쳐 생육이 가장 양호한 LYH201 균주를 야생균주로 선발하였다. Bergey's manual 방법으로 특성을 조사한 결과 LYH201균주는 Gram 양성균으로 내생포자 형성, oxidase, CMCCase, xylanase, catalase, V-P test, methyl red test, arabinose 등의 탄소원 이용성은 양성이었으며, indole을 생성하고, urease, lecithinase, dihydrolase test는 음성이었고, 전자현미경으로 관찰한 결과 길이가 $2.5{\sim}3.0{\mu}m$, 너비가 $0.5{\sim}0.7{\mu}m$의 간균인 Bacillus subtilis로 확인되었다. Bacillus subtilis LYH201 균주를 MMY를 이용하여 배양한 결과 온도 $50^{\circ}C$, pH 6.0에서 생육이 가장 좋았다.

• 한국식품위생안전성학회지
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• 제23권3호
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• pp.248-256
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• 2008

본 연구는 축산물 유래 L. monocytogenes에 대한 역학적 연구로서 분리균의 hemolysin(LLO) 및 lecithinase(LCP)생산성, Congo red dye(CRA)흡수성 및 hemolysin activity를 조사하는 한편 inlA, inlBV, actA, hlyA, plcA 및 plcB의 virulence gene을 PCR법으로 분석하였다. LLO, LCP 및 CRA의 양성률은 L. monocytogenes의 경우 68균주 중 각각 100%, 94.1% 및 77.9%이었고, L. ivanovii와 L. seeligeri를 제외한 다른 Listeria spp.(L. innocua, L. gray, L. murrayi, L. welchimeri)는 음성이었다. LLO와 LCP간에는 통계적인 유의성은 없었으나 CRA는 약간 낮게 나타났으며(p<0.05), serotype 1/2b 및 4b 간에도 유의성이 인정되지 않았다. 면양적혈구에 대한 용혈성(MHU)에서 L. monocytogenes의 경우 2배에서 16배까지 다양한 반응을 보였으나 L. ivanovii와 L. seeligeri를 제외한 다른 Listeria spp.는 음성이었다. hemolysin activity(HU)는 L. monocytogenes의 경우 대부분의 균주가 1.0 HU/mg 이상이었으나 다른 Listeria spp.는 대부분 0.04 HU/mg 이하였다. PCR 증폭하여 virulence gene을 분석한 결과 모든 L. monocytogenes는 각기 예상한 크기의 PCR 증폭산물이 검출되어 hlyA, plcA, plcB, inlA 및 inlB gene을 보유하고 있음이 확인되었으나 다른 Listeria spp.는 어떠한 증폭산물도 보이지 않았다. 또한 actA gene에 대한 증폭산물은 385bp와 268bp 크기의 2종류로 각각 57.4%와 42.6%의 분포를 나타내었다. actA gene의 size 분포에서 국내산 쇠고기, 닭고기, 유가공장에서는 큰 size가 많았는데 반하여 미국산 수입쇠고기에서는 작은 size가 많은 것으로 나타났다.