• Plant Resources
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• v.8 no.3
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• pp.286-292
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• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.150-160
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• 2006

This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the leaves and genetic variation of the leaves of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean leaf length and width were 6.09cm and 3.36cm, respectively. Mean petiole length was 1.08cm, and mean shoulder width and incision depth were 1.50cm and 0.84cm, respectively. Mean leaf base angle was $98.56^{\circ}$. Leaf index and petiole length were the most important components of leaf characteristics. Two groups were clustered in distance level 5.4. The result of the cross between Pyungsung and Bulsae are as follows: Mean leaf length and width were 5.25cm and 3.10cm, respectively. Mean petiole length was 0.87cm, and mean shoulder width and incision depth were 1.50cm and 0.76cm, respectively. Mean leaf base angle was $107.87^{\circ}$, which was the widest among ten cross combinations. Two groups were clustered with seven leaf characteristics in distance level 4.2. Group I included two small groups, and group II included four small groups.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.333-339
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• 2017

This research was aimed to establish rapid analysis technique for the determination of nitrate ($NO_3{^-}$) concentration in the leaves of paprika, which has key role for the stable vegetative and reproductive growth. Leaf petiole and blade sap of two paprika cultivars ('Raon red' and 'Raon yellow') were used for the determination of $NO_3{^-}$ concentration, separately using rapid detection kit (RQ-flex) and spectroscopy quantification methods. In addition, two paprika cultivars namely, 'Nicole' and 'TP2001' were used to determine the status of $NO_3{^-}$ concentration in leaf of each fruiting group. $NO_3{^-}$ concentration in leaf blade sap and the content in leaf showed significant correlation ($R^2=0.8628$), analysed by RQ-flex and spectroscopy methods, respectively. Furthermore, leaf petiole sap and the content in leaf also showed significant correlation ($R^2=0.6734$) but the relationship was poor compared to leaf blade sap and the leaf content. $NO_3{^-}$ concentration in petiole sap decreased in all the cultivars from early to late fruiting group. The higher concentration in the lower leaves and the continuous decrease towards the upper leaves in the both years were found through the analysis of $NO_3{^-}$ concentration in different leaf position. In addition, daily short-term fluctuation of $NO_3{^-}$ in petiole sap could be rapidly monitored. These results showed that long-term or short-term monitoring by test strip-based rapid analysis technique might be useful tool for the diagnosis of nutritional status for the stable of nutritional management in paprika.

• Journal of Life Science
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• v.8 no.6
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• pp.623-626
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• 1998

Conditions for somatic embryogenesis and plant regeneration from stem tissues of Euphorbia pulcherrima were esta-blished. Explants from leaf, petiole, stem were examined for their embryogenesis on MS solid medium supplemented with plant growth hormones in combination at various concentrations. From leaf or petiole explants, callus was indu-ced well but never proceeded to the embryonic stage in our expermental conditions. From stem explants, however, multiple shoots following callus induction emerged in about 6 to 8 weeks on MS agar medium supplemented with 1.5 mg/L of benzyladenine. Upon transfer, roots were developed on hormone-free MS solid medium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.245-255
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• 1994

Weak persistence of white clover (Trifolium repens L.) under continuous grazing management has been limited its availability in the mixture with grasses. The experiment was carried out to determine the effect of defoliation interval on the regrowth and morphological characters of the clover cultivars. Individual plants of Regal (large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 15cm plastic pot containing a 1: 2: 1 soil:sand:Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. For the analysis of the cultivar response, plants were sampled on the final harvest date(0), and 1, 3, 7, 14 and 28 days after the final harvest date. Harvested dry weight of all cultivars declined as defoliation interval was increased, and that of Regal was the highest compared to the other cultivars. During the regrowing period, increase of total plant dry weight was due to that of leaf and petiole dry weight, and that of Osceola was greater than the others. Although total leaf area and mean single leaf area were increased during the regrowing period, they were reduced with increased defoliation interval and those of Osceola were the greatest until 14-days regrowth. S184 possessed the most number of leaves and Osceola did the longest petiole since 14-days regrowth although the more defoliation, the less nunber of leaves per plant and the shorter petiole. Stolon length and growing tips of all cultivars increased steeply during regrowth, while they were decreased with increased defoliation interval and those of S184 were the highest. More frequent defoliation had detrimental effects on regrowth of white clover, although larger leaf type was productive but less persistent in a mixture with grasses than smaller leaf type.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.316-325
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• 2001

To develope a technique for efficiently managing fertilizer for cucumber, a quick test method to quantify nitrate content in soil solution and leaf petiole juice using a simple instrument was investigated. Among the nitrate analyzing instruments such as compact ion meter, nitrate ion meter, and test strip with reflectometer, the paper test-strip used in conjunction with a hand-held reflectometer was most closely correlated with ion chromatography method in nitrate content, and then it would be suggested with a tool that a farmer can use rapidly, conveniently and accurately for nitrate analysis in a field. Nitrate content in soil solution collected by porous cup was very variable on the lapsed time after drip irrigation and the sampling positions such as soil depth and the distance from dripper. As a result, a significant correlation between nitrate contents of soil solutions and 2M KCl soil extract was not found. However, nitrate content in soil solution extracted with a volume basis (soil:water=1:2) showed the highly significant correlation with that in 2M KCl extract. Nitrate contents of cucumber leaf petiole juices was greatly different between upper and lower leaves. Eleven to sixteen positioned-leaf would be a proper sampling position to determine nitrate content in leaf petiole for evaluating nutrient state by plant tissue analysis. From the secondary regression equations between nitrate contents of soil and petiole juice and the yield of cucumber, nitrate levels for real time diagnosis were estimated as $400mg\;l^{-1}$ soil solution by porous cup. $300mg\;l^{-1}$ in a soil volume extraction, and $1400mg\;l^{-1}$ in petiole juice from spring to summer season. In addition, the maximum yield of cucumber fruit in pot test was obtained in nitrate $1500mg\;l^{-1}$ level of petiole juice, which was similar to nitrate $1400mg\;l^{-1}$ in greenhouse trial.

• Plant Resources
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• v.8 no.3
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• pp.235-243
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• 2005

The purpose of this research is to establish basic database on Cornus kousa by way of investigation, analysis and comparison of characteristics of leaf and flower, then fostering good cultivar on each morphological characteristics - leaf length, leaf width, petiole length, left lateral vein, right lateral vein. Leaf length and leaf width in Mt. Jiri shows big tendency which is 83.5 mm, 52.4 mm each in comparison with the whole mean of 72.5 mm, 41.2 mm, whereas populations of Suwon and Mt. Halla has leaf length of 66.0 mm, 65.7 mm - 9.8%, 10.4% lower value in comparison with seven mean population; leaf width is 38.4 mm, 35.3 mm - 7.3%, 16.7% lower than whole mean and shows lowest tendency among seven selected populations. Long width of flower and short width of flower in Boeun shows big tendency which is 99.9mm, 96.5 mm each in comparison with the whole mean of 76.0 mm, 73.6 mm, whereas populations of Mt. Halla has 50.1 mm, 48.2 mm which shows lowest tendency. On petal length, petal width and length of flower petiole, Boeun populations have bigger and Mt. Halla shows little tendency. The measurement result of flower colors on each population by using Spectrum Color Mater shows followings: populations of Mt. Duckyoo and Mt. Halla shows lower lightness than any other populations, but wholly shows higher lightness which is refers brightness in seven selected populations. Thus it shows peculiarities of white flower color.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.206-213
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• 1998

This experiment was conducted to investigate the effects of concentration of NO$_{3}$$^{[-10]}$ , $K^{＋}$ and $Ca^{＋＋}$ in nutrient solution on the seedlings growth of ‘Mudeungsan’watermelon. Seeds were sown on March 16, 1997. NO$_{3}$$^{[-10]}$ , $K^{＋}$ and $Ca^{＋＋}$ was treated with three different levels, respectively, NO$_{3}$$^{[-10]}$ = 106, 206, 406ppm; $K^{＋}$ = 150, 200, 400 ppm; $Ca^{＋＋}$ = 150, 200, 400 ppm. Plant growth was investigated at four-days interval in 10 days after treatment. Increasing N concentration in the nutrient solution increased the plant height, leaf area, number of leaves, fresh and dry weight of shoot while high concentration of K and Ca in the nutrient solution decreased the seedling growth in terms of leaf area, leaf dry weight. The uptake of N, K and Mg in petiole sap was positively affected by the adding of 206 ppm of nitrate, 150 ppm of potassium and 200 ppm of calcium levels, respectively. The P and Ca uptake in petiole sap was not affected by the N and K concentrations in the nutrient solution while K concentration above 200 ppm decreased the N and Mg content in petiole sap. High concentration of Ca in the nutrient solution increased the Ca content in petiole sap while Ca concentration above 200 ppm decreased the N content.

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.89-92
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• 2007

The purpose of this study was to establish basic database on Sorbus alnifolia by investigating, analyzing and comparing characteristics of leaf, and then foster good cultivar on each morphological characteristics - leaf length, leaf width, petiole length, left lateral vein, right lateral vein. Leaf length and leaf width in Osan showed distinct tendency in comparison with the whole mean : 103.4mm and 63.3mm, 86.4mm and 62.9mm, respectively. Populations of Mt. Dukyoo and Mt. Halla showed leaf length of 52.8mm and 58.2mm respectively 32.6% and 39% lower than the mean of ten population. Leaf width of Mt. Gwangyang and Mt. Chink was 52.8mm and 51.9mm, respectively 17.6% and 16.2% lower than whole mean, respectively, and showed the lowest tendency among ten selected populations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.480-488
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• 1994

Low seedling growth rates of white clover (Trifolium repens L.) have been limited its good establishment to pastures. The experiment was done to determine the effect of removal of cotyledon and unifoliolate on the growth and morphological characters of contrasting white clover cultivars for 8 weeks after the treatment. Individual plants of cv. Regal (large leaf), Louisiana S-l (medium-large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 10cm plastic pot containing a 2:1:1 soil:sand:peat moss mixture until the cotyledon or unifoliolate stage and then removed one (C1) or two cotyledons (C2) at cotyledon stage, and unifoliolate only (U), unifoliolate and one cotyledon (DC1) or unifoliolate and two cotyledons (DC2) at the unifoliolate stage. To measure the removal effect on biomass and morphological characters (leaf area, petiole and stolon lengths, growing tips and leaves), plants were sampled 4, 6 and 8 weeks after the treatment. Intact plants had greater biomass and morphological characters than removal-treated ones, Removal treatments at cotyledon stage, C1 and C2, were decreased more biomass and morphological characters than removal ones at unifoliolate stage while the severer cotyledon removal, the more reduction. Stolon length per plant and petiole length markedly inclined 6 weeks after the treatments although biomass and the other characters continuously did. Relatively large-leaved cultivar had more biomass, leaf area per plant and longer petiole than the other(s) but the reverse results were true in stolon length, growing tips and no. of leaves per plant. Biomass was linearly increased with increased leaf area but the earlier and severer removal, the less slope. The severer damage of cotyledon and unifoliolate had detrimental effects on the growth and aftermath establishment of white clover