• Food Science and Preservation
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• v.7 no.4
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• pp.362-365
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• 2000

Changes in eleutherosides by havest time and part of Acanthopanax koreanum Nakai were investigated. Eleutherosides contents in stem root and leaf of Acanthopanax koreanum harvested at September were 549.2 ppm 483.2 ppm and 255.9 ppm, respectively. Eleutheroside E content in stem was 653.1 ppm, which was higher than that in root, but it was not detected in leaf. As the trees were grown eleutheroside B and eleutheroside E contents were increased gradually. Eleutheroside B content of 5 year's age tree was 567.3 ppm, which was 1.6 times higher than that of 1 year's age tree.

• Korean Journal of Organic Agriculture
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• v.7 no.1
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• pp.115-127
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• 1998

Under the visual judgement of consumers, to reduce nitrate intake through vegetables, this experimentation analyzed the content of nitrate, in heading leaf vegetables such as chinese cabbage(Brassica campestris L. ssp. perkinensis (Lour.) Rupr), cabbage(Brassica oleracea L. var. capitata) and lettuce(Lactuca sativa L.) by the leaf number. And the result is summarized as follows In the nitrate content change by the leaf number, the nitrate content is increased as it goes by from inner leaf to outer leaf and the nitrate content in leaf midrib is higher than that in leaf blade. In case of chinese cabbage, the nitrate content in the leaf midrib from the most inner leaf to the most outer leaf changed 40-3,177ppm and in the leaf blade it changed 40-2,887ppm. But the nitrate content in the leaf blade of cabbage from the most inner leaf to the most outer leaf changed 89~2,297ppm and in the leaf blade it changed 25~765ppm. In case of lettuce, the nitrate content change of the leaf midrib by the leaf position was 419~4,349ppm, and in the leaf blade it changed 260~2894ppm. It was conclude that the outer leaf of chinese cabbage, cabbage and lettuce should be removed to keep the lower nitrate intake by population before it is consumed.

• Journal of Korean Society of Forest Science
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• v.99 no.5
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• pp.726-735
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• 2010

Publications with the data on allometric equation, biomass and productivity of major oak forests in Korea were reviewed. Different allometric equations of major oak species showed site- or speciesspecific dependences. The biomass of major oak forests varied with age, dominant species, and location. Aboveground tree biomass over the different oak species was expressed as a power equation of the stand age. The proportion of tree component (stem, branch and leaf) to total aboveground biomass differed among oak species, however, biomass ranked stem > branch > leaf in general. The leaf biomass allocation over the different oak species was expressed as a power equation of total aboveground biomass while there were no significant patterns of biomass allocation from stem and branch to the aboveground biomass. Tree root biomass continuously increased with the aboveground biomass for the major oak forests. The relationship between the root to shoot ratio and the aboveground tree biomass was expressed by a logarithmic equation for major oak forests in Korea. Thirteen sets of data were used for estimating the net primary production (NPP) and net ecosystem production (NEP) of oak forests. The mean NPP and NEP across different oak forests was 10.2 and 1.9 Mg C $ha^{-1}year^{-1}$. The results in biomass allocation, NPP and NEP generally make Korean oak forests an important carbon sinks.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.186-193
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• 2021

BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11^th, 15^th, 19^th, and 23^rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.239-246
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• 2013

The purpose of this study was to find out the potential of the Mosidae(Adenophora remtriflora) leaf, one of the natural ingredients, for the cosmetics by measuring their antioxidant functions and skin improving effects. The result of the phytonutrient of the Mosidae leaf test shows, that the heat extract of Mosidae leaf DPPH has 9% higher ability than Ethanol extract.(p<0.05), but significant a difference was not found between the hydroxyl Radical of Mosidae leaf heat extract (65.22%) and Ethanol extract(63.50%). Mosidae leaf heat extract(40.82mg/mL) has much lower polyphenol compound than Ethanol extract(47.90mg/mL)(p<0.05). A clinical test of Mosidae leaf powder pack was performed for a group of 16 middle aged women(age between 35-60) from October 20 to November 17, 2011. Control group was used on the right cheek, and test group was used on left cheek for comparison. The procedure was twice a week for 4 weeks to identify and grade level of moisture, roughness, pore size and spot. After 4 weeks of testing, there were significant improvements in moisture enhancement and spot(p<0.05), subtle change in roughness and the size of the pores. Therefore, we confirmed skin improvement effect of Mosidae leaf as new cosmetic material.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.4
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• pp.605-610
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• 1992

Two experiments were conducted to investigate into the effects of replacing maize with a mixture of cassava root and leaf meal (CRLM) on the performance of broiler chickens. In experiment 1, CRLM replaced 50 or 100 percent of maize in the control diet. In experiment 2, the 100 percent CRLM based-diet was fortified at a further 3 percent level with coconut oil. A total of 180, one-day old Shaver Starbro chicks, raised up to 49 days of age, were used. There were no significant (p<0.05) differences in final body weights, feed intake and feed efficiency between the control group and the group fed the diet in which 50 percent of the maize was replaced with CRLM. At the 100 percent level of replacement of maize with CRLM, however, final body weights, feed efficiency and carcass yields, were significantly (p<0.05) reduced. Dressing percentage and feed intake were not affected (p>0.05) by level of substitution of maize with CRLM. In experiment 2, when the diet in which all of the maize was replaced with CRLM was fortified with coconut oil at an additional 3 percent level, broiler performance improved and equaled (p=0.05) that obtained on the control maize diet. It was concluded the CRLM can replace 50 percent of maize in broiler diet without adversely affecting performance. However, for the complete replacement of maize with CRLM, it is necessary to further supplement such a diet with a high energy density ingredient if broiler performance is to be maintained.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.75-78
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• 2001

The whitefly, Dialeuropora decempuncta Quaintance and Baker (Homoptera : Aleyrodidae) causing considerable damage to mulberry, Morus alba. Crop loss caused by the whitefly was estimated in mulberry. Quantitative yield loss was estimated on the basis of harvest data from both sprayed and unsprayed plots. Data on pest incidence has been recorded at weekly intervals in both sprayed and unsprayed plots from 30 th day of plant age till harvesting i.e., 60 days after pruning in August October season. An attempt has been made to establish a relationship between whitefly population and percentage of crop loss due to it. Percentage of crop loss due to whitefly has got a linear relationship with the whitefly population. An initial population of 24 adults/top leaf would be able to cause 24% loss (l,630 Kg leaf/ha ) in a period of 30 days. Economic analysis postulates that application of pesticide can stove a net amount of 1,630 Kg leaf/ha which is sufficient to produce 67.65 Kg of additional multivoltine cocoons. This determines the cost/benefit ratio which will enable to workout the economics of management practices.

• Korean Journal of Organic Agriculture
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• v.13 no.1
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• pp.101-112
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• 2005

In order to select the suitable soybean cultivars for paddly field cultivation, growth response and yields characters were investigated with 37 Korean domestic soybean cultivars, in Yeoncheon, Gyeonggi province. The results can be summarized as follows ; When the soybeans were cultivated in paddy field, significant differences were observed in growth responses at each growth stage and yields among the cultivars. Yields of six cultivars, such as Danbaekkong, Paldokong, Pungsannanulkong, Myeongjunamulkong, Saeolkong, and Namhaekong, were less than 200kg/10a, however, seven cultivars, such as Shinpaldalkong 2, Sohokong, Dormikong, keumkangkong, Bukangkong, Dajangkong, and Geomjeongkong 2, showed highest yields ranged from 301~385kg/10a. At V5 stage, highly positive correlation(r=091$^{{\ast}{\ast}}$) was observed between the top dry weight and leaf area while negative correlation was existed between T/R ratio and root dry weight. However, at R2 age, leaf area was correlated with both top and root dry weight while T/R ratio was correlated with leaf area and top dry weight. Thirty-seven soybean cultivars used in this experiments were classified into 3 different clusters based on 19 important agronomic characters, and genotypic properties of the cultivars were similar in each cluster. In results, high yields could be achieved by selected cultivation of suitable soybean cultivars such as Shinpaldalkong 2 which showed early maturation date and highly ripening ratio.

• Journal of Sericultural and Entomological Science
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• v.41 no.3
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• pp.129-134
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• 1999

The purpose of this study is to investigate the effects of the ice-cream containing mulberry leaves on glucose levels in blood and on sensory characteristics. Mulberry leaves were treated with sodium hydrogencarbonate (NaHCO3) to remove the fresh green note out of dried mulberry powder with ball mill. The recovery rate of dried mulberry leaf powder was 28.9% from fresh mulberry leaves. The points of ice-cream with (treatment 2 : milk-fatty 23%) and without (treatment 7 : coconut butter 9.5%) milk-fatty in the sensory characteristics were 8.31 and 8.47, respectively, of 9.00 as a full point. The range of age of volunteers taken part in the glucose level test were 21∼25 years old. The glucose levels in blood were measured before and after taking up ice-cream. In case of ice-cream with vanilla, the glucose levels increased 27.8%, while those in the up take of ice-cream with mulberry powder decreased 1.6%. In conclusion, the present study has demonstrated that ice-cream containing mulberry leaf powder has an effect to decrease blood glucose levels after the application.

• Korean Journal of Weed Science
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• v.18 no.1
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• pp.69-75
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• 1998

Responses of tobacco(Nicotiana tabacum cv. Xanthi) leaves of different age to diphenyl ether herbicide oxyfluorfen were evaluated with respect to cellular leakage, chlorophyll loss, and membrane lipid peroxidation. When tobacco leaves of different age were incubated under light condition at $25^{\circ}C$ following 12hr dark incubation. Significant electrolyte leakage from the treated tissues into the bathing medium occurred. The change of electrolyte leakage was proportional to the oxytluorfen concentration and the duration of light exposure to the tissues. Electrolyte leakage from the tissues treated with oxyfluorfen was highly dependent on the leaf age. From the tissues of younger age, more electrolyte leakage occurred and lag period was greatly reduced. Chlorophyll loss and membrane lipid peroxidation, as measured by malondialdehyde production, caused by oxyfluorfen treatment were also dependent an the age of treated leaf tissues. In conclusion, physiological responses of tobacco leaves to oxyfluorfen greatly varied with the age of treated tissues, and thus tobacco plants could be used as appropriate materials for studying the mechanisms of tolerance to diphenyl ether herbicides.