• Title/Summary/Keyword: Layer-by-Layer

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Breeding of New Synthetic Egg Production Line in Domestic Chicken by Introducing Sex Linked Gene II. Selection of Superior Lines from Cross Breeding (성 감별 유전자를 도입한 다산계계통 신품종 육종에 관한 연구 II. 우량 교배조합 선발 시험)

  • 오봉국;손시환;조윤연
    • Korean Journal of Poultry Science
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    • v.20 no.1
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    • pp.1-9
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    • 1993
  • This study carried out to build up new synthetic egg production lines which had sex-linked gene for feather color sexing and also superior combining ability for producing the best commercial chicks. The closed flock breeding method was utilized to improve the general performances in the first experiment and combining ability for heterosis was tested for new synthetic line in the second experimental year. In order to test for the egg production ability in cross breeds synthetic lines, the crossing of B$\times$4 B$\times$C, two imported strains and two domestic strains as controls were compared for the general performances. There was on difference in mortality, body weight to 56 weeks of age. Sexual maturity was delayed about 10 days by comparing with other reports, except 153 days of the Manina White, but no difference among mating systems in this experiment. The hen housed egg production in B$\times$A, B$\times$C was 186.3, 191.3 respectively and it was better than the other controls, except ISA imported lines. The hen-day egg production of B$\times$A, B$\times$C was better than other controls, with 75.7%, 76.8% respectively. In the average egg weight, the B$\times$C cross breed was highest with 64.5g. As the sex of hatching chicks was identified by difference of feather color, the genetic composition of synthetic lines must be homogenized. The feather color of female chicks was brown and that of male was silver (99%), In conclusion, the egg production ability of B$\times$A, B$\times$C cross breeds was superior to the imported and domestic lines. Therefore, it suggest that the synthetic lines with sex-linked gene might be utilized for improving egg production performances.

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Effects of Dietary Organic Selenium Levels on Performance and Selenium Retention in Broiler Chickens and Laying Hens (유기태 셀레늄의 첨가가 육계 및 산란계의 생산성 및 셀레늄 축적에 미치는 영향)

  • Na, J.C.;Kim, S.H.;Jang, B.G.;Kim, J.H.;Yu, D.J.;Kang, G.H.;Kim, H.K.;Lee, D.S.;Lee, S.J.;Lee, J.C.;Lee, W.J.
    • Korean Journal of Poultry Science
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    • v.33 no.4
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    • pp.255-262
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    • 2006
  • Two experiments were conducted to investigate the effect of dietary organic selenium levels on performance and selenium retention in broiler chickens and laying hens. In experiment 1, the effects of dietary organic selenium levels on the weight gain, feed intake, feed conversion, and selenium retention of meat and liver in broiler chickens were investigated. For each growth phase, the basal diet was supplemented with 0 (control), 0.60, 1.20, 1.80 and 2.40 ppm Se from selenium yeast(SY). Weight gain, feed intake, and feed conversion were not affected by the selenium addition in diets. Breast muscle Se levels were linearly increased (P<0.05) as dietary Se level increased by SY. Selenium concentration of liver tissue was increased (P<0.05) in supplemental SY compared to the control, and was increased (P<0.05) in supplemental 1.20, 1.80 and 2.40 ppm SY compared to the 0.60 ppm SY. In Experiment 2, 12-week-experiment using Hy-Line laying hens (68 wk of age) was conducted to examine the effects of dietary organic selenium on egg Production, egg weight, daily egg mass, feed intake, feed conversion, egg quality, and selenium concentration of eggs. A corn-soybean meal basal diet was supplemented with 0 (control), 0.30, 0.60, 0.90 and 1.20 ppm Se from selenium yeast (SY). Egg Production was significantly improved(P<0.05) in supplemental 0.30 and 0.90 ppm SY compared to the control and 0.60 ppm SY during week 1 to 12, but daily egg mass, feed intake, and feed conversion showed no difference in supplemental SY and control. Haugh unit, yolk color and eggshell breaking strength showed no difference in supplemental SY and control. Eggshell thickess was significantly (P<0.05) higher in supplemental 0.60 and 1.20 ppm SY compared to the 0.90 ppm SY in week 9. Egg Se levels were linearly increased (P<0.05) as dietary Se level increased by SY.

A Geophysical Study on the Geotectonics and Opening Mechanism of the Ulleung Basin, East Sea (동해 울릉분지의 지구조 및 성인에 관한 지구물리학적 연구)

  • Suh, Man-Cheol;Lee, Gwang-Hoon;Shon, Ho-Woong
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.1
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    • pp.34-44
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    • 1998
  • Analysis of gravity, magnetic, and seismic reflection data from the Ulleung Basin, East Sea has provided some insights into the opening mechanism and crustal type of the basin. Free-air gravity anomaly data show positive anomalies of about 40~60 mgal near the Korea Plateau and Oki Bank and of about -20~20 mgal in the central basin. Bouguer gravity anomaly data exhibit NE-SW trending positive anomalies of about 150 mgal in the central basin which is interpreted to be related to high-density crustal material. Abrupt changes in both Free-air and Bouguer gravity anomaly profiles across the basin margins may be due to transition between continental and oceanic crusts. Magnetic anomalies in the basin are generally less than -400 nT. No stripe pattern is evident in the magnetic anomaly map but a NW-SE trending symmetric pattern is seen in some magnetic profiles. The symmetric pattern is probably associated with the high-density crustal material in the central basin suggested by Bouguer gravity anomaly. The acoustic basement in the deep part of the basin has only a small amount of local relief. No graben or half-graben structures are seen in the acoustic basement from which mechanical extension might be inferred. The lack of high-relief structures in the acoustic basement may suggest that the basin is underlain by oceanic crust or that the basement is overlain by thick volcanic layer which obscures the structures and relief of the basement. High-density crust in the central basin inferred from gravity data, abrupt changes in gravity anomalies across the basin margins, symmetric pattern seen in some magnetic anomaly profiles, and lack of relief in the acoustic basement may suggest sea-floor spreading origin of the Ulleung Basin.

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Development of an Solid Separation System for Pig Slurry (돈 슬러리용 고형물 분리시스템 개발)

  • 김민균;김태일;최동윤;백광수;박진기;양창범;탁태영
    • Journal of Animal Environmental Science
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    • v.8 no.1
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    • pp.9-16
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    • 2002
  • This study was conducted to develope the new solid separating system which can be efficiently and economically removed the solid parts in high pollutants concentration of pig slurry. The pollutants concentration, BOD$_{5}$ , COD and SS of the slurry used in this study was 15,990($\pm$2,389)mg/l, 20,004($\pm$5,512)mg/l and 26,486($\pm$5,935)mg/l, respectively. After removal of solid part in slurry, the pollutants concentration, BOD$_{5}$, COD and SS was change into 5,617($\pm$690)mg/l, 5,553($\pm$633)mg/land 1,456($\pm$341)mg/l, respectively in the Fixed biological membrane tank. The reduction of the pollutants concentration of suspend liquid through membrane will be allowed to greatly improve the water purification by an Activated sludge method. This separating system consisted of a temporary storage, a circulating tank and a Fixed Biological membrane tank. A temporary storage which has a draining system of screw type and an aeration device played a tremendous role in draining the solid by filled an aeration of 0.3 l/min. A Fixed Biological membrane tank of which a styrofoam filled in a 2/3 volume as a Biological media was fixed by a stainless steel net (pore size : 0.5mm) to separate the liquid layer of influx in them. The separating system efficiency factors were the speed of screw motor, cycle number of slurries in a circulating tank and moisture contents of solid effluent through the screw path. Although the pollutants concentration was very variable in temporary storage, the final concentration of $BOD_5$ and SS, except COD of the suspended liquid in a Fixed biological membrane were not different regardless of cycle number of a circulating tank. Moisture contents of effluent from temporary storage was 73% under the speed 1 ppm of screw motor and 62% under the 1/4rpm of it.

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Effects of Several Amendment Materials on Salt Accumulation and Kentucky Bluegrass (Poa pratensis L.) Growth in Sand Growing Media Established Over the Reclaimed Saline Soil (염해지 토양을 기반으로 조성된 모래 지반구조에서 토양개량제 종류에 따른 토양내 염류 집적과 켄터키 블루그래스(Poa pratensis L.)의 생육)

  • Rahayu, Rahayu;Yang, Geun-Mo;Choi, Joon-Soo
    • Asian Journal of Turfgrass Science
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    • v.25 no.2
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    • pp.208-216
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    • 2011
  • The purpose of this study was to find soil-amendment materials those support the growth of Kentucky bluegrass and reduce salt accumulation at the sand based growing media in saline conditions. Rootzone profile in columns consisted of 20 cm of top soil, 20 cm coarse sand as capillary rise interruption layer and 10 cm reclaimed paddy soil as the base of the profile. Top soils were mixtures of dredged sand (DS) and amendment with compositions of 90% sand + 10% peat moss (SP), 80% sand + 10% soil + 10% bottom ash (SSoBa), 80% sand + 20% soil (SSo), 90% sand + 5% peat + 5% zeolite (SPZ), and 80% sand + 20% bottom ash (SBa). The top soil mixtures of DS and amendments were treated with and without gypsum (Gp). The columns were soaked into 5 cm depth saline water reservoir with the salinity level of $3-5dSm^{-1}$. Irrigation of $2dSm^{-1}$ saline water with rate of $5.7mm\;day^{-1}$ was applied by 3 day interval. Application of zeolite decreased SAR, application of gypsum decreased ECe of the sand amended by peat + zeolite and decreased the SAR of sand amended by bottom ash. The SP and SSoGp resulted in higher clipping dry weight of Kentucky bluegrass. The SSoGp and SPZGp showed longer root lengths. The SP and SBaGp showed higher visual quality. Addition of gypsum to soil and bottom ash treatments resulted in the increased shoot growth, whereas additional gypsum to the treatments of peat, soil and zeolite increased the root growth of Kentucky bluegrass.

Forest Community Structure of Mt. Bukhan Area (북한산 지역의 삼림군집구조에 관한 연구)

  • 박인협;이경재;조재창
    • Korean Journal of Environment and Ecology
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    • v.1 no.1
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    • pp.1-23
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    • 1987
  • To investigate the forest structure of Mt. Bukhan. ranging from Seoul to Kyongkido, twenty plots were set up by the vegetation physiognomy and vegetation analysis was carried out. According to the leading dominant tree species in canopy stratum, forest communities were classified into three large groups of natural forest communities, semi-natural forest communities and artificial forest communities, and each of them covered 82.64, 7.03, and 5.71% of Mt. Bukhan area, respectively. Pure or mixed natural forest communities of Pinus densiflora and Quercus mongolica were major forest communities and covered 70.8% of Mt. Bukhan area. The important planted tree species were Robinia pseudoacacia, Pinus rigida, and Alnus birsuta and they were mainly planted at the southern slope and roadside. The degree of human disturbance of vegetation of 8, 7, and 6 area covered 82.64, 0, and 12.74%, respectively. According to forest dimensions, most of forest communities were young aged forests of which mean DBH was 20cm and canopy height below 10m. However, a few mature forest communities of Pinus densiflora or Quercus mongolica were found in the small area. The range of Shannon's species diversity of major natural forest communities, pure or mixed forest communities of Pinus densiflora and Quercus mongolica was 1.085~1.242. According to stand dynamic analysis by DBH class distribution, the present Quercus mongolica communities arid Robinia pseudoacacia communities may last long their present forest structure and most of other communities may be succeeded to Quercus mongolica communities, however, a few communities invaded by Robinia pseudoacacia and Quercus aliena-Quercus acutissima communities may be succeeded to Robinia pseudoacacia communities and Quercus aliena communities, respectively. DCA was the most effective method of this study. DCA ordination were showed that successional trends of tree species seem to be from Pinus densiflora through Quercus serrata. Prunus sargrntii. Sorbus alnifolia to Q. mongolica. Fraxinus mandsburica, F. rhynchophylla in the upper layer and from Zanthoxylum schinifolium, Lespedeza crytobotrya trough Rhus trichocarpa. Rh. verniciflua. Rhododendron mucronulatum. Rh. schlippenbachii to Acer pseudo-sieboldianus. Magnolia sieboldii, Euonymus sieboldianus.

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Major, Trace and Rare Earth Element Geochemistry, and Oxygen-Isotope Systematics of Illite/smectite in the Reindeer D-27 Well, Beaufort-Mackenzie Basin, Arctic Canada (카나다 보포트-맥켄지 분지의 일라이트/스멕타이트의 원소 지화학 및 산소동위원소 연구)

  • Ko, J.;Hesse, R.;Longstaffe, F.J.
    • Economic and Environmental Geology
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    • v.28 no.4
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    • pp.351-367
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    • 1995
  • The elemental geochemistry and oxygen isotopes of illite/smectite (I/S) have been studied in relationship to the mineralogical trend in the Reindeer D-27 well, Beaufort-Mackenzie Basin. The increase in concentrations of $K_2O$, Rb and rare earth elements (REE), the decrease in concentrations of tetrahedral elements such as Mg, Ti, Sc, Zn and Zr, and the increase in concentrations of tetrahedral elements such as Be and V can be related to I/S compositions that vary systematically with depth. Layer formulae of S- and I-layers are estimated as $[Al_{1.57}Fe_{.19}Mg_{.31}Ti_{.07}][Si_{3.84}Al_{.16}]O_{10}(OH)_2$ and $[Al_{1.84}Mg_{.16}][Si_{3.33}Al_{.67}]O_{10}(OH)_2$, respectively. The mobilization of REE appears to occur during illitization. The increase in concentrations of REE, especially La and Ce, with depth is probably linked to incorporation of ions with high valency (e.g. $V^{5+}$) in tetrahedral sites. The excess valency due to V is partly counter-balanced by ions with low valency (e.g. $Be^{2+}$) and, in turn, the local valency deficiency caused by $Be^{2+}$ could be compensated by high-charge interlayer cations such as REE (+3). ${\delta}^{18}O$ values of I/S range from 2.91 to 15.72‰ (SMOW), and increase with depth, contrasting to trends observed in the Gulf Coast and elsewhere. The increase in ${\delta}^{18}O$ of I/S results from the rapid increase in ${\delta}^{18}O$ of pore water that overcomes the decrease in temperature-dependent fractionation values with increasing burial depth (${\delta}^{18}O_{pore\;water}>-d{\Delta}/_{I/S-water};\;d{\delta}^{18}O_{I/S}>0$). Calculated ${\delta}^{18}O$ values of pore water in equilibrium with I/S suggest that the original water was probably meteoric water. The stratification of pore water is postulated from the presence of an isotopically light interval, about 450m thick. The depth range of the isotopically light zone overlaps, but does not coincide with the interval of lowered I-content and $K_2O$ concentrations, suggesting that oxygens may have been exchanged independently of mineralogical and geochemical reactions.

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The Dietary Effects of Marigold Extracts on Egg Production, Egg Quality and the Production of Lutein Fortified Chicken Eggs (사료 내 매리골드 추출물의 첨가 급여가 계란 생산성과 계란 품질 및 난황 내 루테인 함량에 미치는 영향)

  • Kim, Eun-Jib
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.135-142
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    • 2014
  • This study was conducted to evaluate dietary effects of Marigold extract on laying performance, egg quality, oxidative stability of egg yolk and lutein transfer into chicken eggs. A total of one-hundred eighty nine 55-wk-old Hy-Line Brown layers were divided into seven groups and fed control diet or each experimental diet containing 0.1, 0.3, 0.5, 1.0, 1.5 or 2.0% Marigold extract. Egg production, egg weight and daily egg mass were not affected by dietary treatments. The yolk colors in groups fed diets containing Marigold extract were significantly higher than that of control. The Haugh unit were tended to be improved by feeding of diets containing Marigold extract although there were no significant difference in egg shell strength and thickness. The MDA (malondialdehyde) contents in groups fed diets containing Marigold extract above 0.5% were significantly reduced than that of control. After 14d of storage, the Haugh unit values in groups feed diets containing 0.3 and 1.0% Marigold extract were significantly higher than that of control (p<0.05). The concentration of lutein in egg yolk increased by feeding of Marigold extract. When 2% Marigold extract was supplemented to the diet, lutein content of egg was increased as much as 1.71 mg/60 g. These results indicated that the use of Marigold extract in layer diets was effective in egg quality and for the production of lutein fortified eggs.

Reliability Verification of FLUKA Transport Code for Double Layered X-ray Protective Sheet Design (이중 구조의 X선 차폐시트 설계를 위한 FLUKA 수송코드의 신뢰성 검증)

  • Kang, Sang Sik;Heo, Seung Wook;Choi, Il Hong;Jun, Jae Hoon;Yang, Sung Woo;Kim, Kyo Tae;Heo, Ye Ji;Park, Ji Koon
    • Journal of the Korean Society of Radiology
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    • v.11 no.7
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    • pp.547-553
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    • 2017
  • In the current medical field, lead is widely used as a radiation shield. However, the lead weight is very heavy, so wearing protective clothing such as apron is difficult to wear for long periods of time and there is a problem with the danger of lethal toxicity in humans. Recently, many studies have been conducted to develop substitute materials of lead to resolve these problems. As a substitute materials for lead, barium(Ba) and iodine(I) have excellent shielding ability. But, It has characteristics emitting characteristic X-rays from the energy area near 30 keV. For patients or radiation workers, shielding materials is often made into contact with the human body. Therefore, the characteristic X-rays generated by the shielding material are directly exposured in the human body, which increases the risk of increasing radiation absorbed dose. In this study, we have developed the FLUKA transport code, one of the most suitable elements of radiation transport codes, to remove the characteristic X-rays generated by barium or iodine. We have verified the reliability of the shielding fraction of the structure of the structure shielding by comparing with the MCPDX simulations conducted as a prior study. Using the MCNPX and FLUKA, the double layer shielding structures with the various thickness combination consisting of barium sulphate ($BaSO_4$) and bismuth oxide($Bi_2O_3$) are designed. The accuracy of the type shown in IEC 61331-1 was geometrically identical to the simulation. In addition, the transmission spectrum and absorbed dose of the shielding material for the successive x-rays of 120 kVp spectra were compared with lead. In results, $0.3mm-BaSO_4/0.3mm-Bi_2O_3$ and $0.1mm-BaSO_4/0.5mm-Bi_2O_3$ structures have been absorbed in both 33 keV and 37 keV characteristic X-rays. In addition, for high-energy X-rays greater than 90 keV, the shielding efficiency was shown close to lead. Also, the transport code of the FLUKA's photon transport code was showed cut-off on low-energy X-rays(below 33keV) and is limited to computerized X-rays of the low-energy X-rays. But, In high-energy areas above 40 keV, the relative error with MCNPX was found to be highly reliable within 6 %.

Mammalian Cloning by Nuclear transfer, Stem Cell, and Enzyme Telomerase (핵치환에 의한 cloning, stem cell, 그리고 효소 telomerase)

  • 한창열
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.6
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    • pp.423-428
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    • 2000
  • In 1997 when cloned sheep Dolly and soon after Polly were born, it had become head-line news because in the former the nucleus that gave rise to the lamb came from cells of six-year-old adult sheep and in the latter case a foreign gene was inserted into the donor nucleus to make the cloned sheep produce human protein, factor IX, in e milk. In the last few years, once the realm of science fiction, cloned mammals especially in livestock have become almost commonplace. What the press accounts often fail to convey, however, is that behind every success lie hundreds of failures. Many of the nuclear-transferred egg cells fail to undergo normal cell divisions. Even when an embryo does successfully implant in the womb, pregnancy often ends in miscarriage. A significant fraction of the animals that are born die shortly after birth and some of those that survived have serious developmental abnormalities. Efficiency remains at less than one % out of some hundred attempts to clone an animal. These facts show that something is fundamentally wrong and enormous hurdles must be overcome before cloning becomes practical. Cloning researchers now tent to put aside their effort to create live animals in order to probe the fundamental questions on cell biology including stem cells, the questions of whether the hereditary material in the nucleus of each cell remains intact throughout development, and how transferred nucleus is reprogrammed exactly like the zygotic nucleus. Stem cells are defined as those cells which can divide to produce a daughter cell like themselves (self-renewal) as well as a daughter cell that will give rise to specific differentiated cells (cell-differentiation). Multicellular organisms are formed from a single totipotent stem cell commonly called fertilized egg or zygote. As this cell and its progeny undergo cell divisions the potency of the stem cells in each tissue and organ become gradually restricted in the order of totipotent, pluripotent, and multipotent. The differentiation potential of multipotent stem cells in each tissue has been thought to be limited to cell lineages present in the organ from which they were derived. Recent studies, however, revealed that multipotent stem cells derived from adult tissues have much wider differentiation potential than was previously thought. These cells can differentiate into developmentally unrelated cell types, such as nerve stem cell into blood cells or muscle stem cell into brain cells. Neural stem cells isolated from the adult forebrain were recently shown to be capable of repopulating the hematopoietic system and produce blood cells in irradiated condition. In plants although the term$\boxDr$ stem cell$\boxUl$is not used, some cells in the second layer of tunica at the apical meristem of shoot, some nucellar cells surrounding the embryo sac, and initial cells of adventive buds are considered to be equivalent to the totipotent stem cells of mammals. The telomere ends of linear eukaryotic chromosomes cannot be replicated because the RNA primer at the end of a completed lagging strand cannot be replaced with DNA, causing 5' end gap. A chromosome would be shortened by the length of RNA primer with every cycle of DNA replication and cell division. Essential genes located near the ends of chromosomes would inevitably be deleted by end-shortening, thereby killing the descendants of the original cells. Telomeric DNA has an unusual sequence consisting of up to 1,000 or more tandem repeat of a simple sequence. For example, chromosome of mammal including human has the repeating telomeric sequence of TTAGGG and that of higher plant is TTTAGGG. This non-genic tandem repeat prevents the death of cell despite the continued shortening of chromosome length. In contrast with the somatic cells germ line cells have the mechanism to fill-up the 5' end gap of telomere, thus maintaining the original length of chromosome. Cem line cells exhibit active enzyme telomerase which functions to maintain the stable length of telomere. Some of the cloned animals are reported prematurely getting old. It has to be ascertained whether the multipotent stem cells in the tissues of adult mammals have the original telomeres or shortened telomeres.

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