• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1634-1638
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• 2002

Quantification of endogenous amino acid loss at the terminal ileum is an essential means for calculation of the true amino acid digestibility of a feedstuff. Since nitrogen appeared in the determined diet or not could shift the results very much, also, none of digestibility markers could be recovered with 100% rate at the terminal ileum, the objectives of the present study were: (1) to determine endogenous amino acid losses when fed either a casein diet or a protein-free diet and (2) to examine the reliability of chromic oxide or acid insoluble ash in the protein-free diet. Six ileal-cannulated pigs ($65{\pm}1.85 kg$ BW) with a simple T-cannula in the terminal ileum were used in a replicated $3{\times}3$ Latin square designed trial, after allowed a 14 d recuperation period. Each test period ran for 12 days comprised of a 10 d adjustment period and a 2 d collection period. The endogenous AA losses of His, Ile, Lys, Cys, Thr, Val, Trp, Asp, Glu, and Ser from pigs fed the casein diet were significantly higher than those of the protein-free diet (p<0.05). No significant difference was found in the amount of endogenous amino acid loss when determined with the different markers in the protein-free diet (p>0.05). These data suggest that endogenous amino acid loss could be underestimated when a protein-free diet is used. A direct effect of dietary peptides on the endogenous amino acid loss was found when the casein diet was fed. Our results also indicate that acid insoluble ash can be used as an inert marker as an alternative to chromic oxide when measuring endogenous amino acid loss.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.816-824
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• 2005

The effects of the nonionic surfactant Tween 80 and a mixture of fibrolytic enzymes on total tract digestion, in situ disappearance (ISD) and ruminal fermentation characteristics of orchardgrass hay and barley grain were investigated in a 4${\times}$4 Latin square experiment with 4 non-lactating Holstein cows and 4 diets in 4 periods. Cows were offered a total mixed ration of 50% rolled barley grain and 50% orchardgrass hay treated with either 1) water (control), 2) 0.2% (vol/wt) Tween 80, 3) 0.2% (vol/wt) hydrolytic enzyme, or 4) 0.2% hydrolytic enzyme plus 0.2% Tween 80. Total tract digestibility coefficients of DM, nitrogen, NDF and ADF were not affected (p>0.05) by dietary treatment. Compared to the control, the rate of ISD of DM from orchardgrass hay was faster (p<0.05) in cows receiving diets treated with the enzyme alone or with enzyme plus Tween 80 (0.06/h vs. 0.076 and 0.069/h). The rate of digestion was lower (p<0.05) as compared to control when barley grain was treated with these additives. Ruminal fluid pH and concentrations of total VFA, acetate, isobutyrate and butyrate were not affected (p>0.05) by treatments. Cows that consumed diets treated with enzyme plus Tween 80 had higher (p<0.05) ruminal concentrations of propionate and isovalerate, and lower (p<0.05) acetate:propionate ratios. Compared to the control, microbial protein synthesis tended (p = 0.13) to increase with the addition of enzyme to the diet while nonammonia nitrogen flow to the duodenum increased (p<0.05) with both enzyme and Tween 80 treatments. The study indicated that fibrolytic enzymes alone or in combination with Tween 80 could enhance ISD of orchardgrass hay and ruminal concentrations of propionate, valerate and iso-valerate, but do not affect total tract digestibility.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1040-1048
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• 1999

Four ruminally fistulated crossbred $(Sahiwal{\times}Holstein\;Friesan)$ adult (~6 yr.) cattle of about $318{\pm}16kg$ body weight were randomly assigned in an experiment based on $4{\times}4$ latin square design to study the effect of different level of barseem (Trifolium alexendrinum) supplementation to wheat straw based diet on intake, digestion, VFA production, eating behaviour and passage rate. Four dietary treatments were wheat straw ad lib. (I), supplementation to wheat either 15(II), 30(III) or 45(IV)% of berseem. Mean total volatile fatty acids (TVFA) concentration in rumen liquor was 58.45, 66.14, 77.92 and 78.64 mmol/l. TVFA concentration in rumen liquor increased significantly (p<0.01) with increased level of berseem supplementation upon 30% level of breseem. Two peaks of TVFA concentration was observed at 4 and 8 h post feeding. Brseem supplementation showed no significant effect on daily time spent for eating, ruminating or idling, chews per minute or number of rumination boli ingested per minute. Time spent for eating and ruminating one kg neutral detergent fibre (NDF) was significantly (p<0.05) less in breseem supplemented groups. Animals in groups I, II, III and IV consumed 26, 34, 47 and 57% of DM within 1st 4 h and 64, 70, 70 and 77% of total DM within 1st 8 h of offering. All the animals consumed more than 90% of their DMI within 12 h. Active period of rumination was observed 8h post feeding reached the peak at 16 h post feeding, then declined and animals spend considerable time idling in last 4 h of 24 h feeding cycle. Berseem supplementation showed no significant effect on eating and rumination time. It is concluded that berseem supplementation upon 30% increases the efficiency of chewing during eating and rumination, which results in increase intake and TVFA production and nutrient digestibility.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.666-672
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• 2000

Two experiments were conducted to determine the ileal digestibility of the amino acids contained in sunflower meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred ($Yorkshire{\times}Landrace{\times}Beijing$ Black) barrows were fitted with simple Tcannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75% sunflower meal. For the growth trial, 80 crossbred ($Yorkshire{\times}Landrace{\times}Beijing$ Black) growing pigs (21.5 kg) were fed corn-soybean meal diets supplemented with 0, 5, 10 or 15% sunflower meal. Five pens (2 gilts and 2 castrates) were assigned to each treatment. With the exception of arginine and valine, the digestibility coefficients for the indispensible amino acids declined as the level of sunflower meal in the diet increased. During both the growing (21.5-49.1 kg) and finishing (49.1-90.3 kg) periods and over the entire experiment (21.5-90.3 kg), average daily gain declined in a linear manner (p<0.05) with increasing amounts of sunflower meal. Feed intake was not significantly altered while feed conversion declined in a linear manner (p<0.05) during the grower period only. When the entire experimental period was taken into account, there would appear to be little penalty in either growth or feed conversion for including sunflower meal at levels up to 10% of the diet. Therefore, the price relationship between sunflower meal and other high-protein feedstuffs may provide an excellant opportunity for pork producers to use sunflower meal in order to reduce feed costs.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.313-316
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• 2000

Three Holstein steers, fitted with ruminal and duodenal cannulas, were used in a replicated $3{\times}3$ Latin square design to determine the digestibility of dietary nitrogen in total digestive tract by three methods, 1) mobile nylon bag (MNB); 2) total fecal collection (TFC); and 3) washed fecal sample after freezing and thawing through a sieve with a pore size of $45{\mu}m$ (WFS). A basal diet of oaten hay-barley was supplemented with one of the following protein sources; soybean meal, fish meal or blood meal. Steers were fed at a level of 2% of body weight. The experimental diets were contained approximately 1.85% nitrogen. There were no differences (p>0.05) among the diets on DM, NDF and nitrogen disappearances, and the diet results were pooled to assess the methods. Total tract disappearances of dry matter and neutral detergent fiber were 61.6, 71.1 and 78.9 and 25.3, 63.2 and 64.6 for MNB, TFC and WFS methods, respectively. The lower digestibility of DM and NDF in the MNB method could be a result of low ruminal incubation time. The TFC method had the lower (p<0.05) determination of nitrogen disappearance in the total digestive tract than the MNB and WFS methods. On the other hand, nitrogen disappearance in the total digestive tract determined by the WFS technique was comparable to that in MNB technique, as there was no significant difference (p>0.05) between the methods. It is shown that the disappearance of dietary nitrogen in the total digestive tract could be estimated in the intact animals by using washed fecal sample prior to freezing and thawing.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.2
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• pp.213-219
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• 2000

Two experiments were conducted to determine ileal digestibilities for the amino acids contained in sesame meal using the regression technique and then applying the values obtained in a growth trial using growing-finishing pigs. For the digestibility trial, four, 20 kg crossbred (Yorkshire $\times$ Landrace $\times$ Beijing Black) barrows were fitted with a simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75 percent sesame meal. For the growth trial, 210 crossbred (Yorkshire $\times$ Landrace $\times$ Henan Min) growing pigs ($21.8{\pm}1.4kg$), were fed corn-soybean meal based diets supplemented with 0, 3, 6, 9, or 12% sesame meal. Three pens (7 gilts and 7 castrates) were assigned to each treatment. With the exception of arginine and phenylalanine, the digestibility coefficients for the indispensible amino acids declined as the level of sesame meal in the diet increased. There was little agreement between the amino acid digestibilities determined with the regression technique and values previously published for sesame meal determined with the direct method. Daily gain and feed conversion both declined (linear effect p=0.02 and 0.06 respectively) as the level of sesame meal in the diet increased.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.4
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• pp.470-473
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• 2000

The experiment was carried out to evaluate the effect of varying levels of flaked com abomasally infused on energy metabolism and nitrogen metabolism in fattening steers. The starch levels of flaked corn of abomasally infused were 0, 300, 600 and 900 g/d. Four mature fattening steers fitted with permanent abomasum cannulas were allocated to a $4{\times}4$ Latin square design were fed at 1.2 maintenance requirement a basal diet of Chinese wildrye (Aneurolepidium Chinense). Compared with 0 g/d (control group), digestible nitrogen, retention nitrogen (RN, g/d) and the efficiency of digestible nitrogen converted into retention nitrogen (RN/DN, %) of 300, 600 and 900 g/d groups were higher (p<0.05). The post-ruminal starch digestion of flaked corn were 71.36, 80.27 and 64.71 % when the amounts abomasally infused were 300, 600 and 900 g/d, respectively. When the amount of starch abomasally infused was more than 600 g/d, the post-ruminal digestion of starch decreased. 300, 600 and 900 g/d starch infusion groups showed higher metabolizable energy intake (ME) and net energy gains (NEg, MJ/d) than the control group, and the efficiencies of metabolizable energy converted into body weight (Kf, %) of these groups were higher than the control group by 38.31, 73.18 and 67.06% (p<0.05). Kf (Y, %) had a positive curved relation to starch of flaked com abomasally infused (X, g/d), $Y=36.1605X^{0.0760}$ (n=16, r=0.9308).

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.369-375
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• 2000

Two experiments were conducted to determine the apparent ileal digestibility of the amino acids contained in peanut meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred (Yorkshire${\times}$Landrace${\times}$Beijing Black) barrows were fitted with simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75% peanut meal. For the growth trial, 200 crossbred (Yorkshire${\times}$Landrace${\times}$Beijing Black) growing pigs (20.9 kg) were fed corn-soybean meal diets supplemented with 0, 5, 10, 15 or 20% peanut meal. Five pens (4 gilts and 4 castrates) were assigned to each treatment. With the exception of arginine, histidine and phenylalanine, the digestibility coefficients for the indispensible amino acids declined as the level of peanut meal in the diet increased. There was a good agreement between the amino acid digestibilities for lysine, methionine, threonine and tryptophan determined using the regression technique and amino acid digestibilities previously published for peanut meal. During both the growing (21-54 kg) and finishing (54-99 kg) periods, the addition of peanut meal decreased average daily gain (p=0.01) and feed conversion in a linear manner (p<0.05). Feed intake was not significantly different among treatments. The overall results suggest that peanut meal can be used at levels up to 15% in diets fed to growing-finishing pigs provided that the diet has been balanced for digestible amino acids.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.4
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• pp.521-527
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• 2000

Two experiments were conducted to determine the ileal digestibility of the amino acids contained in cottonseed meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred ($Yorkshire{\times}Landrace{\times}Beijing$ Black) barrows were fitted with simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed com-soybean meal based diets supplemented with 0, 25, 50 or 75% cottonseed meal. For the growth trial, 128 crossbred ($Yorkshire{\times}Landrace{\times}Beijing$ Black) growing pigs (21 kg) were fed com-soybean meal diets supplemented with 0, 4, 8 or 12% cottonseed meal. Four pens (4 gilts and 4 castrates) were assigned to each treatment. The digestibility coefficients for the indispensible amino acids declined as the level of cottonseed meal in the diet increased. There was good agreement between the amino acid digestibilities for methionine, threonine and tryptophan determined using the regression technique and amino acid digestibilities previously published for cottonseed meal. However, for lysine, the value of 67% ileal digestibility obtained in the present experiment was higher than most previously published estimates which ranged from 42 to 73%. During both the growing (21-43 kg) and finishing (43-84 kg) periods, the addition of cottonseed meal decreased average daily gain and feed conversion in a linear manner (p<0.05). Feed intake was not significantly different between treatments. The overall results suggest that cottonseed meal can be used at levels of up to 8% in diets fed to growing-finishing pigs provided that the diet. has been balanced for digestible amino acids.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1755-1762
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• 2003

Two experiments were conducted to determine effects of particle size in total mixed ration (TMR) on performance of lactating cows. Three rumen cannulated Holstein cows were used in a $3{\times}3$ Latin square design for the metabolic experiment. The particle size of the diets was determined using the Penn State Particle Size Separator (PSPSS) and weighing the proportion of sample remaining on the top screen (19 mm diameter). The 3 treatments were short, medium or long diets (4.9, 24.2 and 27.8% of sample remaining on the top screen of the PSPSS, respectively). Nine farms in the Edmonton area were surveyed and the farms were placed into groups based on the particle size of the ration fed. The groups were short ${\leq}6%$, medium 7-12% and long ${\geq}13%$ of sample weight remaining on the top screen of the PSPSS. Dry matter intake was greater (p=0.07) for the medium diet than the long diet in the metabolic study and resulted in a higher (p=0.07) efficiency of milk production. On the commercial farms, a significantly (p=0.002) lower milk fat percentage was observed for the long diet compared to the short diet. The results of these studies confirm that forage particle size influences milk composition and milk fat was negatively correlated to TMR particle size.