• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.364-370
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• 1997

Four local cattle were ($145{\pm}9.7kg$) used in a $4{\times}4$ Latin square design to study the effect of different levels of rumen degradable protein (RDP) in straw based ration on purine derivatives excretion and microbial N supply in cattle. The four rations were formulated at the same amount of energy but varying RDP approximately 50 (U0), 75 (U1), 100 (U2) and 150 (U3) percent levels of RDP requirement for maintenance. They were fed ranged from 101 to 304 g RDP/d. Apparent digestibility of all nutrients increased significantly (p < 0.01) in cattle fed ration U2 than other rations. Rumen $NH_3-N$ concentration increased from 43 to 130 mg/l in response of RDP intake. Purine derivatives excretion increased significantly (p < 0.01) with incremental level of 203 g RDP/d (U2) intake and positively correlated (r=0.69, p < 0.01, n=16) with amount of RDP intake. The rates of rumen microbial N supply were 16.8, 27.2, 39.1 and 32.9 g/d for rations U0, U1, U2 and U3 respectively. Efficiency of microbial N supply (EMNS) per kg of DOMR were 19.0, 25.3, 33.0, and 28.6 g and per MJ of ME. Intake were 0.62, 1.00, 1.44 and 1.21 g for U0, U1, U2 and U3 respectively and highest results were obtained in cattle fed U2 ration. Results of this study suggest that PD excretion and EMNS were increased as incremental level of RDP intake (U2) in local cattle.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.391-397
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• 1997

Six medium-framed steers, fitted with ruminal cannulas, were utilized in a $6{\times}6$ Latin square design with a $3{\times}2$ arrangement of treatments to determine the effects of sorghum hybrid and grain supplementation on nutrient digestibilities and passage rates and ruminal metabolism of silage-based diets fed to growing steers. The diets consisted of three wholes-plant silages (a high grain-containing, grain sorghum and middle-season, moderate grain-containing, and late-season, low grain-containing forage sorghums), each fed with or without 25% rolled grain sorghum. No significant interactions occurred between sorghum hybrid and grain supplementation for the digestion or passage rate criteria measured. Ruminal butyrate concentration was the only fermentation characteristic affected by a hybrid ${\times}$ grain supplementation interaction. The grain sorghum silage diets had the highest DM, OM, and ADF digestibilities; the late-season silage diets, the lowest. Digestibility of NDF tended to be highest (p < 0.10) for the grain sorghum silage, whereas starch digestibility was not affected by sorghum hybrid. Ruminal ammonia, acetate, propionate, butyrate, and total VFA concentrations were highest for the grain sorghum silage diets. Grain supplementation increased DM and OM digestibilities, but had no effect on digestibilities of NDF, ADF, and starch. Ruminal pH was decreased, but total VFA concentration and acetate : propionate ratio were not affected by grain supplementation.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.725-732
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• 2007

The effects of supplementing Gamba grass (Andropogon gayanus) with varying levels of hay from cassava (Manihot esculenta Crantz) and dried cassava root chip on growth and diet digestibility were studied using local male goats with an average initial body weight of 14.0 kg. Thirty-two animals were allocated to a completely randomized $2{\times}2$ factorial design with eight animals per treatment. The factors were two levels of cassava hay (25% and 35% of an expected dry matter (DM) intake of 3% of body weight) and cassava root chips (0 or 1% of body weight) on an individual basis with grass offered ad libitum. Another four animals were assigned to a $4{\times}4$ Latin square design to study digestibility, and were given the same four diets as in the growth experiment. Total DM intake was significantly higher in the group fed diets with cassava hay and root while the DM intake of Gamba grass was not significantly different between treatments. The supplementation with cassava hay and root increased the apparent digestibility of DM, organic matter and N and resulted in a higher N-retention. The apparent digestibility of neutral detergent fibre and acid detergent fiber was not affected significantly. The average daily gain of animals fed diets supplemented with both cassava hay and root was significantly higher than for the animals supplemented with cassava hay alone. The highest daily gain recorded was 70 g/day. In conclusion, supplementing a basal diet of Gamba grass with cassava hay and root chips improved DM intake, digestibility, N-retention and weight gain. In order to minimize the waste of cassava hay, the inclusion level of cassava hay can be recommended to be 25% of expected DM intake, which would give acceptable intake and growth performance when cassava root is included in the diet.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.378-387
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• 2009

To investigate the effects of sago palm pith (SPP) substitution of corn in the diets on intake, digestibility, rumen fermentation characteristics, nitrogen balance and microbial N Supply, five ruminally fistulated Southern indigenous cattle (mean initial BW = 226${\pm}$5 kg) were randomly assigned to a 5${\times}$5 Latin Square Design to receive five diets, $T_1$ = concentrate with 0% SPP, $T_2$ = 25% SPP, $T_3$ = 50% SPP, $T_4$ = 75% SPP and $T_5$ = 100% SPP, of dietary dry matter, respectively. Plicatulum hay (PH) was offered ad libitum as the roughage. A metabolism trial lasted for 21 days during which liveweight changes and feed intakes were measured. Based on this experiment, there were no significant differences (p>0.05) among treatments groups regarding total DM intake (OMI, NDFI and ADFI) and digestion coefficients of nutrients (DM, OM, CP, NDF and ADF), while total DM intake (% BW) was significantly (p<0.05) higher as higher levels of SPP were incorporated into diets. Rumen parameters (ruminal temperature, pH, glucose, packed cell volume, volatile fatty acid and rumen microorganism populations) were similar among treatments (p>0.05), whereas $NH_3-N$, blood urea nitrogen and molar proportion of propionate concentrations were significantly (p<0.05) higher as higher levels of SPP were incorporated into diets. The amount of N absorption, N retention and microbial protein synthesis were similar among treatments. These results indicate that SPP can be included in diets for Southern indigenous cattle to supply up to 100% of supplemental corn when fed with PH without negative impact on animal performance and it was a good approach in exploiting the use of local feed resources for beef cattle production.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.234-239
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• 2012

The objective of this study was to evaluate the effects of forage level and oil supplement on selected strains of rumen bacteria believed to be involved in biohydrogenation (BH). A continuous culture system consisting of four fermenters was used in a $4{\times}4$ Latin square design with a factorial arrangement of treatments, with four 10 d consecutive periods. Treatment diets were: i) high forage diet (70:30 forage to concentrate (dry matter basis); HFC), ii) high forage plus oil supplement (HFO), iii) low forage diet (30:70 forage to concentrate; LFC), and iv) low forage plus oil supplement (LFO). The oil supplement was a blend of fish oil and soybean oil added at 1 and 2 g/100 g dry matter, respectively. Treatment diets were fed for 10 days and samples were collected from each fermenter on the last day of each period 3 h post morning feeding. The concentrations of vaccenic acid (t11C18:1; VA) and c9t11 conjugated linoleic acid (CLA) were greater with the high forage diet while the concentrations of t10 C18:1 and t10c12 CLA were greater with the low forage diet and addition of oil supplement increased their concentrations at both forage levels. The DNA abundance of Anaerovibrio lipolytica, and Butyrivibrio fibrisolvens vaccenic acid subgroup (Butyrivibrio VA) were lower with the low forage diets but not affected by oil supplement. The DNA abundance of Butyrivibrio fibrisolvens stearic acid producer subgroup (Butyrivibrio SA) was not affected by forage level or oil supplement. In conclusion, oil supplement had no effects on the tested rumen bacteria and forage level affected Anaerovibrio lipolytica and Butyrivibrio VA.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.7
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• pp.956-961
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• 2012

Eight male cattle of Local Yellow breed with an average live weight of 121 kg and an average age of 18 months were used to evaluate the effects of different levels of sun-dried cassava foliage supplementation (Manihot esculenta) on intake, digestibility and N retention. Rice straw ad libitum and para grass (Brachiaria mutica) at 1% DM of BW comprised the basal diet. The study was arranged as a $4{\times}4$ double Latin square design, with cassava foliage contributing 0, 0.8, 1.6 or 2.4 g CP/kg BW. The cattle selected cassava leaves in preference to petioles. Petiole intake decreased from 64 to 48% of offered petioles when the cassava foliage proportion increased from the lowest to the highest level. The cattle consumed all the leaves at the two lower levels of cassava foliage inclusion and 91% at the highest level. Rice straw intake decreased significantly as the level of cassava foliage increased. Intake of DM, OM, NDF, and ADF increased significantly with increasing intake of cassava foliage. Daily DM intake per 100 kg BW increased from 2.7 to 3.2 kg with increasing cassava foliage intake. No effect on CP digestibility was detected when the level of cassava foliage increased. Digestibility of DM, OM, NDF and ADF was significantly higher in the group fed no cassava foliage than in the other groups. N retention increased from 16 to 28 g/d with the first level of cassava foliage inclusion, but levelled out at the two highest levels. N excretion increased in both faeces and urine as a response to higher intake of cassava foliage. Maximum N retention occurred when 40% of total N intake came from cassava foliage (equivalent to 1.3 g CP/kg BW).

• The Journal of the Korea Contents Association
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• v.15 no.9
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• pp.425-435
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• 2015

Though most researchers studying humor in recent years agree that 'incongruity' is essential factor of humor elicitation, they have different views in the course of schema activation in processing humor text. One of the different views on schema activation in processing humor text is wether schemata are activated concurrently or selectively. While concurrent activation view suggests that different schemata are concurrently activated because we perceive them at the same time, selective activation view suggests that different schemata are selectively activated because we use selective attention for their perception. This study was conducted to verify these two different views. We clarified that different schemata were activated in processing of humor text, and we examined whether Vaid's experiment failed or not. Experiment was designed mixed 2 (schema1, schema2) ${\times}$ 3 (setup, incongruity, resolution) ${\times}$ (humor, control) factorial design and latin square counterbalancing. As a result of experiment, we got data that different schemata were activated in the course of 'Incongruity' at the same time. Most of all, the activated schemata were kept activation in the course of 'Resolution'. This result suggest that different schemata were activated concurrently.

• Korean Journal of Agricultural Science
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• v.42 no.3
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• pp.237-244
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• 2015

Four ruminally cannulated Holstein steers (BW $401.0{\pm}2.22kg$) fed TMR containing low protein (CP 9.63 %) as a basal diet were used to investigate the effects of cornell net carbohydrates and protein system (CNCPS) fraction enriched protein feeds on rumen fermentation and blood metabolites. The steers used in a $4{\times}4$ Latin square design consumed TMR only (control), TMR with rapeseed meal (AB1), TMR with soybean meal (B2) and TMR with perilla meal (B3C), respectively. The protein feeds were substituted for 30 % crude protein of TMR intake. For measuring ruminal pH, ammonia-N and volatile fatty acids (VFA), ruminal digesta was sampled through ruminal cannula at 1 h-interval after the afternoon feeding. Blood was sampled via the jugular vein after the ruminal digesta sampling. Different CNCPS fraction-enriched proteins did not affect (p>0.05) ruminal pH except B3C being numerically low compared with the other groups. Ammonia-N and VFA were not significantly different among the experimental groups. Numerically low ammonia-N appeared in the steers fed rapeseed meal even though it contained high soluble N composition (A and B1 fractions). The discrepancy is unclear; however this may be related to low protein level in the diet and/or low DM intake. Blood metabolites were not significantly affected by the protein substitution except for blood urea nitrogen that was significantly (p<0.05) increased.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1585-1590
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• 2002

Thirty female goats of local breed (mature weight 28 kg), weighing 11 to 18 kg, were used in a growth experiment with the objectives to evaluate the effect of different levels of Gliricidia sepium leaves in a diet of Guinea grass (Panicum maximum) on feed intake, weight gain and apparent digestibility. The goats were allotted to five treatments, consisting of 5 levels, 0 (GL-0), 20 (GL-20), 30 (GL-30), 40 (GL-40) and 50% (GL-50) of Gliricidia sepium leaves in the diet based on the energy requirements of the goats. Another six female goats were used to study the digestibility of three dietary treatments, GL-0, GL-30 and GL-50, according to a double $3{\times}3$ Latin square arrangement. The crude protein (CP) content in Gliricidia was higher and the neutral detergent fibre (NDF) and acid detergent fibre (ADF) lower than in the Guinea grass. Increasing the amount of Gliricidia leaves resulted in similar intake of total dry matter (DM), organic matter (OM), NDF and ADF among treatments, but CP intake increased with increasing Gliricidia in the diet. The highest total DM intake was 3.0% of body weight in the GL-20 diet. The highest daily live weight gain was 43 g/day in the GL-30 diet. Inclusion of 30% of Gliricidia leaves improved the apparent DM, OM and CP digestibility compared to 0% or 50% inclusion. NDF and ADF digestibility declined with increasing levels of Gliricidia leaves in the diet.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1573-1578
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• 2002

This study was conducted to investigate the effects of diets that contained different levels of fungal treated wheat straw on the intake, digestibility and performance of lactating cows. Eight primiparous Holstein cows, in late lactation ranging from $170{\pm}10$ days in milk and yielding $14.3{\pm}1.3$ kg/d of fat corrected milk (FCM) were allocated into four diets with 0, 10, 20 and 30% fungal (Pleurotus ostreatus coded P-41) treated wheat straw in a $4{\times}4$ Latin Square experiment. The daily intake of DM, OM, DOM, CP and TDN were not affected by substitution of alfalfa hay with fungal treated wheat straw. Inclusion of the treated straw at different levels in the diet did not affect the digestibility of nutrients, except for the ADF that was significantly (p<0.05) reduced in the diet contained 30% treated straw. The types of the diet did not significantly affect daily milk and FCM production. The milk composition including fat, protein, lactose, solid non-fat (SNF) and total solid (TS) were not statistically (p>0.05) different among the diets. All cows gained weight, but the inclusion of treated straw to the diet significantly (p<0.05) increased the body weight gain and the highest amount was obtained in the diet containing 20% treated. Inclusion of fungal treated wheat straw up to 30% of the diet of lactating cows supplemented with a protein source such as cottonseed meal had not affected the nutrients intake and lactation performance.