• Title/Summary/Keyword: Lateral flow

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Effect of Heat, Pressure, and Acid Treatments on DNA and Protein Stability in GM Soybean (GM 콩 DNA와 단백질의 안정성에 대한 열, 압력 및 산 처리의 영향)

  • Pack, In-Soon;Jeong, Soon-Chun;Yoon, Won-Kee;Park, Sang-Kyu;Youk, Eun-Soo;Kim, Hwan-Mook
    • Korean Journal of Food Science and Technology
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    • v.36 no.4
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    • pp.677-682
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    • 2004
  • Debates on safety of genetically modified (GM) crops have led to mandatory-labeling legislation of GM foods in many countries including Korea. Effects of heat, pressure, and acid treatments on degradation of DNAs or proteins in GM soybean at levels below detection limits of qualitative PCR and lateral flow strip test (LFST) methods were examined. Results showed that genomic DNAs and proteins were degraded into fragment sizes no longer possible for detection of inserted gene depending on thermal, or thermal and pressure treatment period. Detectaability of LFST for toasted meal increased in weakly treated soybean. DNA and protein detection methods were barely effective for detection of GM ingredient after $121^{\circ}C$ and 1.5 atmospheric treatment for 20 min. These results will be useful in determining GM labeling requirements of processed foods.

Detection of LM canola with tolerance to glyphosate and glufosinate-ammonium via the Environmental monitoring in South Korea (자연생태계 모니터링을 통한 glyphosate와 glufosinate-ammonium에 저항성을 가지는 유전자변형 캐놀라의 발견)

  • Shin, Su Young;Jo, Beom-Ho;Moon, Jeong Chan;Lee, Jung Ro;Choi, Wonkyun;Seol, Min-A;Kim, Mi-Jeong;Song, Hae-Ryong
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.479-485
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    • 2016
  • Living modified (LM) crops are imported each year to South Korea as food and feeds, LM canola being one of the imported crops. The cultivation of LM crops is not permitted in South Korea but the import of these crops is increasing. In this study, we surveyed the environmental risk of imported LM canola at 9 provinces, from March 2009 to June 2013. Monitoring of canola was conducted around feed factories, roadsides, harbors, farmhouses, and flower festival regions. From the total of 595 canola samples collected from 1850 monitoring sites, we identified 6 LM canola samples. The LM canola samples were subjected to protein and DNA based analysis. PCR analyses using approved 5 single event primers (T45, MS8, RT73, Rf3 and Topas 19-2) revealed that two crops were glyphosate-resistant LM canolas, and four were glufosinate-resistant LM canolas. This study suggested that environmental monitoring is a useful research tool to manage LM crops unintentionally introduced into the environment in South Korea. This result can be used as a basis for future post-management of canola crops.

Clinical usefulness of rapid antigen test to detect respiratory syncytial virus infection (Respiratory syncytial virus 감염진단을 위한 신속항원검사의 유용성)

  • Kim, Hyung Su;Kim, Hee La;Park, Ki Hyung;Cho, Kyung Soon
    • Clinical and Experimental Pediatrics
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    • v.51 no.10
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    • pp.1071-1076
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    • 2008
  • Purpose : Respiratory syncytial virus (RSV) is the most frequent cause of lower respiratory infections in infants and young children. Early detection allows quarantining of infected inpatients to prevent nosocomial transmission and to choose a treatment. To achieve rapid reporting, to facilitate prompt antiviral therapy, and to avoid unnecessary use of antibiotics, an easy, rapid diagnostic method for RSV is needed. We evaluated a lateral flow immunochromatography (RSV Respi-Strip test) and EIA (Enzyme immuno assay) compared to RT-PCR. Methods : From April 2007 to March 2008, 112 consecutive respiratory specimens (nasopharyngeal aspirates, throat swabs, tracheal aspirates, sputum) from patients who were suffering from the clinical signs and symptoms of respiratory tract infection were enrolled in Busan. A total of 112 patients were tested with RSV Respi-Strip (Corio-BioConcept, Belgium), EIA, and RT-PCR at the same time. Results : Of the 112 specimens tested, the number of children who showed positive results at RT-PCR and Respi-Strip were 45 and 42, respectively. The Respi-Strip rapid antigen test had a sensitivity of 88% and a specificity of 94%. The positive and negative predictive values were 90% and 92%, respectively. The agreement was 83%. Conclusion : In our study, the rapid antigen test had as much sensitivity as any method for detection of RSV. The test has many advantages such as easy performance, simple interpretation, and rapid results. If the rapid antigen test is widely applied in the clinical setting, the may be useful for diagnostic and epidemiological studies of RSV infection.

Resonant Characteristics in Rectangular Harbor with Narrow Entrance (2.Effects of Entrance Energy Loss) (개구부가 좁은 직사각형 항만의 공진 특성 (2.항입구 에너지 손실의 영향))

  • 정원무;박우선;서경덕;채장원
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.11 no.4
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    • pp.216-230
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    • 1999
  • A Galerkin finite element model for the analysis of harbor oscillation has been developed based on the extended mild-slope equation. Infinite elements are used to accomodate the radiation condition at infinity and joint elements to treat the matching conditions at the harbor entrance which include the energy loss due to flow separation. The numerical tests for rectangular harbors with fully or partially open entrances show that the energy loss at the harbor entrance considerably reduces the the amplification ratios at the innermost parts of the harbors and that the amplification ratios decrease considerably with increasing incident wave heights and jet lengths at the harbor entrance. Application of the model to the Gamcheon harbor show that when the incident wave amplitude is small the amplification ratios rather increase when the entrance energy loss is included than when ignored because of the shift of the resonance periods. Even though the entrance energy loss was insignificant for the measured long-period incident waves, it would be of great importance if the incident waves were large as in the attack of tsunamis. The resonance period of the Helmholtz mode at the Gamcheon Harbor was calculated to be 31 minutes, which agrees well with the measured one between 27 and 33.3 minutes. The measured resonance periods between 9.4 and 12.1 minutes and 5.2 and 6.2 minutes were also calculated by the numerical model as 10.4 minutes and 6.6 or 5.6 minutes, indicating good performance of the model. On the other hand, it was shown that a variety of oscillation modes exists in the Gamcheon Harbor and lateral resonances of considerable amplification ratios also exist at the periods of 3.6 and 1.6 minutes as in the Young-II Bay.

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Influence of Intracerebroventricular Isoproterenol on the Renal Function of the Rabbit (가토신장기능에 미치는 측뇌실내 Isoproterenol의 영향)

  • Kim, Haeng-Bae;Choi, Bong-Kyu;Kook, Young-Johng
    • The Korean Journal of Pharmacology
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    • v.18 no.1
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    • pp.1-10
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    • 1982
  • In an attempt to delineate the role of beta-adrenoceptors found to be existing in the brain tissue in the central regulation of renal function, isoproterenol, a ${\beta}-adrenergic$ agonist, was administered directly into a lateral ventricle of the rabbit brain and the changes of renal function were observed. Also, the effects of propranolol, a specific ${\beta}-adrenergic$ blocking agent, and its influence upon the isoproterenol action were studied. Isoproterenol, in doses ranging from 5 to $50\;{\mu}g/kg\;i.c.v.$, elicited antidiuresis which seemed to be related to the decreased renal hemodynamics brought about by the systemic hypotension. With moderate doaes of $15\;{\mu}g/kg$ the antidiuresis was less prominent and there was a tendency toward natriuresis, but with higher doses the natriuretic effect became less evident, overrun by the systemic hypotension. Propranolol, $500\;{\mu}g/kg\;i.c.v.$, produced little effect on the renal function, but it eliminated the antidiuretic action of $50\;{\mu}g/kg$ isoproterenol i.c.v. and reversed it to a diuretic and natriuretic one, along with increases in renal plasma flow and glomerular filtration rate. The systemic hypotension also was markedly attenuated by propranolol pretreatment. Thus, it was evident that the renal action of i.c.v. isoproterenol was not blocked by propranolol and became explicit only when the hypotensive action of isoproterenol which seems to he propranolol-sensitive is removed. Various possibilities to account for this disparity in sensitivity were discussed. It is suggested from these observations that the central ${\beta}-adrenoceptors$ might also be involved in the regulation of renal function along with ${\alpha}-adrenoceptors$, though less significant than the latter.

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Effect of Subsurface Drip Pipes Spacing on the Yield of Lettuce, Irrigation Efficiency, and Soil Chemical Properties in Greenhouse Cultivation (지중 점적관수 호스 설치 간격이 상추 수량, 관수량 및 토양 화학성에 미치는 영향)

  • Park, Jin Myeon;Lim, Tae Jun;Lee, Seong Eun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.683-689
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    • 2012
  • This research was carried out to investigate the effect of installation spacing of subsurface drip irrigation pipe on the mineral content, nutrient uptake, yield of lettuce, water requirement for irrigation, and soil chemical properties in greenhouse cultivation. Semi-forcing and retarding culture were implemented in this experiment, with four treatments containing overhead spray irrigation and three subsurface irrigation lateral spacing intervals of 30, 40, 50 cm at a depth of 30 cm from soil surface, respectively. Each mineral content of lettuce grown under subirrigation system did not show significant difference between treatments, however the uptake of nutrients was lower at 50 cm-distance. The yield was largest in 30 cm-subirrigation (SI), followed by 40 cm-SI, overhead spray, and 50 cm-treatment. Water requirement for irrigation was highest in overhead spray, and it was in reverse proportion to the distance of irrigation pipes. $NO_3$-N content in the soil, at a depth of 10 cm, showed a higher value in 50 cm-SI, followed by 40 cm-SI, overhead spray and 30 cm-SI. Exchangeable K content was highest in 50 cm-SI, Mg was highest in 40 cm-SI, and Ca was lowest in 30 cm-SI. In conclusion, the lettuce yield was not different between 30 and 40 cm-SI, but water requirement for irrigation was lower as the distance of irrigation pipes was further. And it seems to be needed more precise research on this theme, because crop yield and the dynamics of soil minerals in subsurface irrigation can vary with the depth and distance of irrigation pipes, dripper, water flow depending on the soil texture, and plant response to soil minerals.

Numerical study on mechanical and failure properties of sandstone based on the power-law distribution of pre-crack length

  • Shi, Hao;Song, Lei;Zhang, Houquan;Xue, Keke;Yuan, Guotao;Wang, Zhenshuo;Wang, Guozhu
    • Geomechanics and Engineering
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    • v.19 no.5
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    • pp.421-434
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    • 2019
  • It is of great significance to study the mechanical properties and failure mechanism of the defected rock for geological engineering. The defected sandstone modeling with power-law distribution of pre-cracks was built in this paper by Particle Flow Code software. Then the mechanical properties of sandstone and the corresponding failure process were meticulously analyzed by changing the power-law index (PLI) and the number of pre-cracks (NPC). The results show that (1) With the increase of the PLI, the proportion of prefabricated long cracks gradually decreases. (2) When the NPC is the same, the uniaxial compressive strength (UCS) of sandstone increases with the PLI; while when the PLI is the same, the UCS decreases with the NPC. (3) The damage model of rock strength is established based on the Mori-Tanaka method, which can be used to better describe the strength evolution of damaged rock. (4) The failure mode of the specimen is closely related to the total length of the pre-crack. As the total length of the pre-crack increases, the failure intensity of the specimen gradually becomes weaker. In addition, for the specimens with the total pre-crack length between 0.2-0.55 m, significant lateral expansion occurred during their failure process. (5) For the specimens with smaller PLI in the pre-peak loading process, the concentration of the force field inside is more serious than that of the specimens with larger PLI.

Basic study of new concept environment-friendly pile foundations with earthquake resistant foundation and lateral reinforcement on rapid-transit railway bridge (고속철도교 기초 내진 및 수평저항성능 보강형 신개념 친환경말뚝 신공법의 실용화 기초연구)

  • SaGong, Myung;Paik, Kyu-Ho;Lim, Hae-Sik;Cho, Kook-Hwan;Na, Kyung
    • Proceedings of the KSR Conference
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    • 2010.06a
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    • pp.880-894
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    • 2010
  • The Grout injected precast pile is widely used in rapid-transit railway bridge recently. The existing portland cement of well used filling at injected precast method that with low strength and environmental pollution, unstable in which ground water contamination by cement flow out, ground relaxation by water down, decrease of horizontality resistance and durability and load transfer divide etc. In particular, as in rapid-transit railway bridge need to secure safety from different angle with vibration of high speed train, horizontal force when train stop and earthquake. Works of foundation construction consider to requirements of the times to coal yard green growth. Together, new green foundation method for possible economics and securing of reduce the term of works are material to developments. Therefore, we carried out study that it is using and development new concept environment - friendly filling include durability and earthquake resistance, for secure safety and minimize environment pollution. To achieve this, we carried out difference tests that new green fillings of underwater concrete, high liquidity, high viscosity, early stiffness as compared to existing portland cement fillings. As results, new green filling have outstanding application at precast pile method and micropile construction method with vertical bearing capacity, horizontal bearing capacity and many case. From now on we will be looking forward to development of new environment-friendly foundation method from various further studies.

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Applications and Perspectives of Fluvial Biogeomorphology in the Stream Management of South Korea (우리나라 하천 관리에서 생물지형학의 적용과 전망)

  • Kim, Daehyun;Kim, Won;Kim, Eunsuk;Ock, Giyoung;Jang, Chang-Lae;Choi, Mikyoung;Cho, Kang-Hyun
    • Ecology and Resilient Infrastructure
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    • v.7 no.1
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    • pp.1-14
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    • 2020
  • In fluvial and riparian ecosystems, biogeomorphological research has considered the complex, multi-way relationships between biological and hydro-geomorphological components over a wide range of spatial and temporal scales. In this review, we discussed the scope and processes of fluvial biogeomorphology by explaining (1) the multi-lateral interactions between organisms and hydrogeomorphic conditions, (2) the relationships between biodiversity and habitat heterogeneity, and (3) the effects of disturbance on ecosystem patterns. Over time, an organism-landform complex along streams transitions in the sequences of geomorphic, pioneer, biogeomorphic, and ecological stages. Over space, water flow and sediment distributions interact with vegetation to modify channel topography. It is the habitat heterogeneity in streams that enhances riparian biodiversity. However, in the areas downstream of a dam, habitat types and conditions are substantially damaged and biodiversity should be reduced. In South Korea, riparian vegetation flourishes in general and, in particular, invasive species actively colonize in accordance with the changes in the fluvial conditions driven by local disturbances and global climate change. Therefore, the importance of understanding reciprocal relationships between living organisms and hydrogeomorphic conditions will ever increase in this era of rapid climate change and anthropogenic pressure. The fluvial biogeomorphic framework reviewed in this article will contribute to the ecological management and restoration of streams in Korea.

Angiogenic Responce to Transmyocardial Mechanical Reveascularization(TMMR) with Polymer Myocardial Stent (고분자 중합체 심근 스템트를 이용한 기계적 경심근 혈류재건술의 혈관생성 반응)

  • Choi, Ho;Lee, Cheol-Joo;Moon, Kwang-Deok;Kim, Young-Jin;Kang, Joon-Kyu;Hong, Jun-Wha;Jee, Kyung-Soo;Han, Man-Jung;Cho, Sang-Ho
    • Journal of Chest Surgery
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    • v.33 no.6
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    • pp.494-501
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    • 2000
  • Background: Transmyocardial laser revascularization(TMLR) for revascularizing ischemic myocardium in patients was originally based on the assumption that laser channels remain their patency much longer. But recent studies show that laser channels did not remain open and that TMLR could achieve treatment benefits without long-term channel patency. The angiongencesis is currently thought to be induced by non-specific inflammatory response to mechanical tissue injury. This study is to evaluate hypothesis that various transmyocaridal mechanical revascularization(TMMR) may induce the angiogenic responses similar to that seen with TMLR, and transmyocaridal polymer stent revascularization(TMSR), the polymer stent in the myocardial tissue is hydrolyzed in 2 weeks, may enhance the non-specific inflammatory reaction resulting angiogenesis. Furthermore, polymer myocaridal stent channels remain long-term patency. Material and Method: Eight domestic pigs underwent ligation of the proximal circumflex artery, and 2 weeks later they were randomized to undergo transmycardial acupunctural revascularization (TMPR, Group I) of the left lateral wall with 18-G needle(n=2), to undergo transmyocardial (TMDR, Group II) with industrial 2mm steel drill(n=2), to undergo transmyocardial polymer stent revascularization (TMSR, Group III) after drilling the infarcted myocardium(n=2), the stent is poly(lactic acid-co-glycolic acid), which is self-degradated in the myocardium, and to a control group the ischemic zone was unterated(n=2). All the pigs were sacrificed after 4 weeks TMMR. Sections from the ischemic zone were submitted for vascular endothelial growth factor (VEGF) ELISA and histology. Result: There were makedly increase in the VEGF immunoassay in the ischemic zone of the TMMR group compared to the ischemic zone of the control group(control: each 30.85 and 43.15pg/mg protein, TMPR: each 44.14 and 68.61 pg/mg protein, TMDR: each 65.92 and 78.65 pg/mg protein, TMSR: each 177.39 and 168.87 pg/mg protein). TMSR channels caused greatest VEGF expression than channels made by other group and the polymer stent channels remained vacuole after 4 weeks. Conclusion: Transmyocardial polymer stent revascularization promoted the most angiogenci response by the VEGF immunoassay, although our study did not show the statistical significancy. The channels remained but the flow patency was not verified. Transmyocardial polymer stent revascularization (TMSR) is desirable in future experimental trials and in view of the significant cost implications comparable to that of laser.

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