• International Journal of Oral Biology
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• 제38권1호
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• pp.5-12
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• 2013

Recent studies indicate that reactive oxygen species (ROS) can act as modulators of neuronal activity, and are critically involved in persistent pain primarily through spinal mechanisms. In this study, we investigated the effects of NaOCl, a ROS donor, on neuronal excitability and the intracellular calcium concentration ($[Ca^{2+}]_i$) in spinal substantia gelatinosa (SG) neurons. In current clamp conditions, the application of NaOCl caused a membrane depolarization, which was inhibited by pretreatment with phenyl-N-tert-buthylnitrone (PBN), a ROS scavenger. The NaOCl-induced depolarization was not blocked however by pretreatment with dithiothreitol, a sulfhydryl-reducing agent. Confocal scanning laser microscopy was used to confirm whether NaOCl increases the intracellular ROS level. ROS-induced fluorescence intensity was found to be increased during perfusion of NaOCl after the loading of 2',7'-dichlorofluorescin diacetate ($H_2DCF$-DA). NaOCl-induced depolarization was not blocked by pretreatment with external $Ca^{2+}$ free solution or by the addition of nifedifine. However, when slices were pretreated with the $Ca^{2+}$ ATPase inhibitor thapsigargin, NaOCl failed to induce membrane depolarization. In a calcium imaging technique using the $Ca^{2+}$-sensitive fluorescence dye fura-2, the $[Ca^{2+}]_i$ was found to be increased by NaOCl. These results indicate that NaOCl activates the excitability of SG neurons via the modulation of the intracellular calcium concentration, and suggest that ROS induces nociception through a central sensitization.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3467-3472
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• 2007

The effects of the wall geometry on the spray-wall impingement process of a hollow-cone fuel spray emerging from a high-pressure swirl injector of the Gasoline Direct Injection (GDI) engine were investigated by means of a numerical method. The ized Instability Sheet Atomization (LISA) & Aerodynamically Progressed Taylor Analogy Breakup (APTAB) model for spray atomization process and the Gosman model were applied to model the atomization and wall impingement process of the spray. The calculation results of spray characteristics, such as a spray development process and a radial distance after wall impingement, compared with the experimental ones by the Laser Induced Exciplex Fluorescence (LIEF) technique. It was found that the radial distance of the cavity angle of 90$^{circ]$ after wall impingement was the shortest and the ring shaped vortex was generated near the wall after spray-wall impingement process.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2005년도 제31회 KOSCO SYMPOSIUM 논문집
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• pp.305-313
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• 2005

Measurements of flame length, width and NOx emissions have been conducted to investigate the effect of an acoustic excitation on flame structure in turbulent hydrogen diffusion flames with coaxial air. The resonance frequency of oscillations was varied between 259 ,514 and 728 Hz with power rate of 0.405 and 2.88w. When these frequencies imposed to hydrogen flames, dramatic reduction of flame length and NOx emission was achieved. And acetone planar laser-induced fluorescence technique was used to measure a concentration of the near field of driven axisymmetric jet. The air-fuel stoichiometric line was plotted to investigate the mixing layer and development of air entrainment to fuel jet. Consequently, acoustic excitation on flame could enhance the air-fuel mixing resulting in abatement of NOx emission quantitatively.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2002년도 추계학술대회 논문집
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• pp.292-293
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• 2002

대기 중에서 HOx(OH, H $O_2$) 라디칼은 가장 중요한 산화제로서 광화학 반응의 핵심적 역할을 한다. 이러한 OH 라디칼의 정확한 농도를 측정하기 위한 여러 가지 시도가 있었으나, 그 존재량이 적고 수명이 짧기 때문에 측정에 많은 어려움이 있다. 간접적인 측정에 근거한 모델링과 계산적인 이론 값에 의하면 대류권에서의 전 지구적 평균 OH 라디칼의 농도는 대략 $10^{6}$molecules $cm^{-3}$이고, H $O_2$ 라디칼의 농도는 $10^{8}$molecules $cm^{-3}$정도로 추정된다. (중략)

• 한국자동차공학회논문집
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• 제6권5호
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• pp.174-181
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• 1998

Experimental and analytical studies are presented to characterize the break-up mechanism and atomization processes of the intermittent- impinging-type nozzle. Gasoline jets passing through the circular nozzle with the outlet diameter of 0.4mm and the injection duration of 10ms are impinged on each other. The impingement of fuel jets forms a thin liquid sheet, and the break-up of the liquid sheet produces liquid ligaments and droplets subsequently. The shape of liquid sheets was visualized at various impinging velocities and angles using the planer laser induced fluorescence (PLIF) technique. Based on the Kelvin-Helmholtz wave instability theory, the break-up length of liquid sheets and the droplet diameter are obtained by the theoretical analysis of the sheet disintegration. The mean diameter of droplet is also estimated analytically using the liquid sheet thickness at the edge and the wavelength of the fastest growing wave. The present results indicate that the theoretical results are favorably agreed with the experimental results. The size of droplets decreases after the impingement as the impinging angle or the injection pressure increase. The increment of the injection pressure is more effective than the increment of the impinging angle to reduce the size of droplets.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1082-1086
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• 2014

A multi-channel microchip electrophoresis (MCME) method with parallel laser-induced fluorescence (LIF) detection was developed for rapid screening of H1N1 virus. The hemagglutinin (HA) and nucleocapsid protein (NP) gene of H1N1 virus were amplified using polymerase chain reaction (PCR). The amplified PCR products of the H1N1 virus DNA (HA, 116 bp and NP, 195 bp) were simultaneously detected within 25 s in three parallel channels using an expanded laser beam and a charge-coupled device camera. The parallel separations were demonstrated using a sieving gel matrix of 0.3% poly(ethylene oxide) ($M_r$ = 8,000,000) in $1{\times}$ TBE buffer (pH 8.4) with a programmed step electric field strength (PSEFS). The method was ~20 times faster than conventional slab gel electrophoresis, without any loss of resolving power or reproducibility. The proposed MCME/PSEFS assay technique provides a simple and accurate method for fast high-throughput screening of infectious virus DNA molecules under 400 bp.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2585-2590
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• 2007

In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIF) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIF are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

• 대한기계학회논문집B
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• 제31권7호
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• pp.644-652
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• 2007

In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIE) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIE are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2006년도 제27회 추계학술대회논문집
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• pp.59-63
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• 2006

횡단류 아음속유동장에서 연료의 수직 분사시 나타나는 액적영역의 액적들을 직접사진촬영으로 측정하고 PLLIF 실험을 통하여 얻은 강도 값으로 SMD분포를 측정하였다. 본 연구의 목적은 정상유동에서의 액적들의 크기 및 분포를 관찰하고, 캐비테이션 및 수력튀김 현상에 대한 액적들의 차이를 확인하는 것이다. 실험을 통하여 정상유동의 액적들은 분사차압, 공기의 유속, 침투거리, 인젝터 지름에 대한 하류방향 거리비(x/d)에 의하여 결정되며, 캐비테이션에 의한 난류강도, 유효지름에 따라 차이가 있음을 확인하였다.

• 한국가시화정보학회지
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• 제10권1호
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• pp.34-39
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• 2012

In the present study, oxygen transfer process across gas-liquid interface in a Y-shape micro-channel is quantitatively visualized using the micro laser induced fluorescence (${\mu}$-LIF) technique. Diffusion coefficient of Oxygen ($D_L$) is estimated based on the experimental results and compared to its theoretical value. Tris ruthenium (II) chloride hexahydrate was used as the oxygen quenchable fluorescent dye. A light-emitting diode (LED) with wavelength of 450 nm was used as the light source and phosphorescence images of fluorescent dye were captured by a CMOS high speed camera installed on the microscope system. Water having dissolved oxygen (DO) value of 0% and pure oxygen gas were injected into the Y-shaped microchannel by using a double loading syringe pump. In-situ pixel-by-pixel calibration was carried out to obtain Stern-Volmer plots over whole flow field. Instantaneous DO concentration fields were successfully mapped according to Stern-Volmer plots and DL was calculated as $2.0675{\times}10^{-9}\;m^2/s$.