• Title/Summary/Keyword: Laser Induced Fluorescence

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DEVELOPMENT OF FILTERING SYSTEM OF LASER FLUORESCENCE FOR IMPROVEMENT OF THE DIAGNOSTIC SENSITIVITY FOR DENTAL CARIES (레이저 형광법의 치아우식증 진단감도를 증진시키기 위한 필터링 시스템 개발)

  • Lee, Sang-Ho;Park, Jong-Whi;Kook, Jung-Ki;Lee, Nan-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.2
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    • pp.189-195
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    • 2003
  • The objective of this study is to improve the optical sensitivity of laser fluorescence for detection of incipient enamel caries. An incipient carious lesion was formed in various stages by placing an enamel specimen of a bovine tooth in STPP demineralization solution. After measuring the optical density of the lesion surface by laser fluorescence induced by argon laser and various alter of yellow(500-520nm), amber(520-540nm), orange(540-560nm), and red(560-580nm), the specimen was cut vertically to measure the depth of the lesion using a polarizing microscope. SAS statistical program was used to analyze the relationship between the optical density of the lesion suface and the depth of the lesion. The results were as follows: 1. The optical density of early carious lesion, measured by laser fluorescence with amber and orange filter, and lesion depth observed by polarizing microscope, were increased as demineralization time increased. 2. The correlation coefficient between optical density of the lesion surface and the histological depth of the lesion was the highest in orange filter(r=0.49), followed by amber(r=0.32), yellow(r=0.13) and red(0.01). 3. Regression analysis showed that the most linear relationship between the optical density and the lesion depth was existed in orange filter group. In regard above results, laser fluorescence could be considered to be reliable for optical diagnosis of dental caries.

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The Visualization of Temperature Field for Nanoporous Thin Film using Laser-Induced Fluorescence. (형광 나노포러스 박막의 온도장 가시화)

  • Oh, Young-Su;Baek, In-Gi;Jeon, Pil-Soo;Kim, Hyun-Jung;Yoo, Jai-Suk
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2459-2464
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    • 2007
  • In present study, a temperature field of specimens which was coated with fluorescence dye such as Rhodamine-B(Rh-B) has been measured, based on the fluorescence intensity. Silica(SiO2) nano porous structure with 1um thickness was constructed on a cover glass, and fluorescence dye was digested into these porous thin films. To optimize manufacturing coating process, various solvents, Rh-B concentration, and other chemical materials were applied to fabricate the specimen and all specimens were measured on the various temperature conditions. For the measurement, a 14 bit cooled CCD camera with 1600 by 1200 spatial resolution is equipped with epifluorescence microscope to obtain only fluorescence intensity from 1.2 mm by 0.9 mm field of view of the illuminated coated specimen.

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Micro-LIF measurement of microchannel flow

  • Kim Kyung Chun;Yoon Sang Youl
    • 한국가시화정보학회:학술대회논문집
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    • 2004.12a
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    • pp.65-74
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    • 2004
  • Measurement of concentration distributions of suspended particles in a micro-channel is out of the most crucial necessities in the area of Lab-on-a-chip to be used for various bio-chemical applications. One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1\;to\;3\times3\;or\;5\times5$ pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

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Study on the Characteristics of Laser-induced Fluorescence from Trace Samarium, Europium and Terbium (미량분석을 위한 Sm, Eu과 Tb의 레이저 여기 형광 특성 분석)

  • Lee, Sang-Mock;Shin, Jang-Soo;Zee, Kwang-Yong;Kim, Cheol-Jung
    • Nuclear Engineering and Technology
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    • v.21 no.4
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    • pp.287-293
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    • 1989
  • The purpose of this study was to develop a rapid and effective method of laser-induced fluorescence analysis for thrace amounts of Sm, Eu and Tb in nuclear fuels. The features of the method are the use of the distinct fluorescence wavelengths and the discriminative lifetimes of the respective elements when excited by a pulsed nitrogen laser. Fluorescence signals of the three elements were isolated by adequate selection of the filters or complexing agents (HFA, TTA) or discriminative delay and gate times in the signal processing circuit. It was found that S $m^{+3}$ and E $u^{+3}$ emitted strong fluorescence in the two complexing agent solutions or HFA and TTA. But in the case or T $b^{+3}$, the fluorescence signal was detected only in HFA solution. With respect to the concentrations of S $m^{+3}$, E $u^{+3}$ and T $b^{+3}$, the fluorescence signal intensities gave superior linearities in the range of 5 ppb-10 ppm for S $m^{+3}$, 0.5 ppb-1 ppm for E $u^{+3}$, and 0.1 ppb-300 ppb for T $b^{+3}$, The detection limits obtained were 5 ppb for S $m^{+3}$, 0.1 ppb for E $u^{+3}$, and 0.01 ppb for T $b^{+3}$, respectively.

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Study on the Chemical Speciation of Hydrolysis Compounds of U(VI) by Using Time-Resolved Laser-Induced Fluorescence Spectroscopy (시간분해 레이저 유도 형광 분광학을 이용한 우라늄(VI) 가수분해 화학종 규명 연구)

  • Jung, Euo-Chang;Cho, Hye-Ryun;Park, Kyoung-Kyun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.7 no.3
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    • pp.133-141
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    • 2009
  • Study on the chemical speciation of uranium(VI) species, ${UO_2}^{2+}$, $UO_2(OH)^+$, ${(UO_2)}_2{(OH)_2}^{2+}$, ${(UO_2)}_3{(OH)_5}^+$, has been peformed by using time-resolved laser-induced fluorescence spectroscopy. Speciation sensitivity which depends on the excitation wavelength was investigated. We obtained the speciation sensitivity in the order of $10^{-9}$ M concentration of U(VI) compounds at the excitation wavelength of 266 nm. The fluorescence spectrum and lifetime of ${UO_2}^{2+}$ were carefully measured at pH 1 and ion strength of 0.1 M. The spectrum showed the four characteristic peaks located around 488, 509, 533, 559nm and the fluorescence lifetime of $1.92{\pm}0.17{\mu}s$. The wavelength shifts of fluorescence peaks and the change of lifetimes for uranium hydrolysis compounds were compared with those of ${UO_2}^{2+}$. We report on the characteristic features, the shifts of peaks to the longer wavelength direction and the prolonged lifetimes, in the fluorescence of the U(VI) hydrolysis compounds.

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Effects of MTV on fuel distribution and flame stability in a SI engine (MTV가 연소실내 연료분포 및 화염 안정성에 미치는 영향 연구)

  • Kim, K S.
    • Journal of ILASS-Korea
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    • v.2 no.4
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    • pp.36-46
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    • 1997
  • The present study investigated the effects of MTV(Manifold Throttle Valve) on the fuel distribution and the flame stability in a SI engine at cold, idling condition. For the quantitative measurement of fuel distribution with PLIF method, compensating techniques of various factors such as laser beam nonuniformity, background image and local OTF nonuniformity were developed. As a result, it was found that MTV had a positive effect on the air-fuel mixing and flame stability.

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The Spray Characteristics of Swirl and Slit Injector to DISI Engine Using LIEF and Mie-scattering Method (LIEE와 Mie 산란 방법을 이용한 직분식 가솔린 엔진의 스월 및 슬릿 인젝터의 분무 특성)

  • Lee Kihyung;Hwang Kyumin;Lee Changhee
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.29 no.3 s.234
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    • pp.356-367
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    • 2005
  • The spray characteristics of DISI injector have a great role in gasoline engine efficiency and emission. Thus, many researchers have studied to investigate the spray characteristics of swirl and slit injectors that are used in a DISI engine. In this study, we tried to provide spray parameters, which affect on the spray characteristics such as injection pressure, ambient pressure and ambient temperature. In addition, we calculated $t_{b}\;and\;t_{c}$ to investigate the break up mechanism of test injectors and obtained $C_{v}$ to evaluate the spray characteristics. As the ambient pressure increases in case of slit injector, $C_{v}$ decreases. The laser-induced exciplex fluorescence (LIEF) technique, which is based on spectrally resolved two-color fluorescent emissions, has applied to measure the liquid and vapor phases for on evaporating spray simultaneously. The TMPD/naphthalene proposed by Melton is used as a dophant to detect exciplex signal. The temporal and spatial distribution of liquid and vapor phases during the mixture formation process was measured by this technique. In the LIEF technique, the vapor phase is detected by the monomer fluorescence while the liquid phase is tracked by the exciplex fluorescence. From this experiment, we found that the spray area of the vapor phase is increased with elapsed time after injection and the area of liquid is decreased when the ambient pressure is 0.1MPa. However, the area tends to increase until the end of injection when the ambient pressure is 1.0MPa.

Experimental Study on the Natural Convection in an Enclosure with an Adiabatic Body Using the Two-Color LIF Method (Two-color LIF 기법을 이용한 단열체가 있는 밀페계 내에서의 자연대류에 관한 실험적 연구)

  • Kwo S. H.;Jeong E. H.;Min Y. U.;Kim K. C.
    • Journal of the Korean Society of Visualization
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    • v.3 no.1
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    • pp.90-96
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    • 2005
  • Most of the previous studies on natural convection were based on the numerical analysis, and some experimental studies considered the classic case of thermal convection. In this study, an adiabatic square body was located at the center of an enclosure between the bottom hot and top cold walls. And the measurement of the detailed temperature fields was conducted by the method of two-color Laser Induced Fluorescence using a Nd:Yag laser. As a result, heat transfer characteristics of natural convection with an adiabatic body was estimated as a function of time over a range of Rayleigh numbers.

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