• Journal of Microbiology and Biotechnology
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• v.32 no.10
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• pp.1325-1334
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• 2022

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as Pseudanabaena mucicola GO0704 through 16s RNA analysis. The potential exploitation of P. mucicola GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from P. mucicola GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.520-525
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• 2019

Mines are an important infrastructure for securing resources, but safety problems can arise in the course of operation. Recently, the mining process is very complicated due to the large scale and mechanization. Therefore, it is necessary to construct accurate geospatial information on mine for systematic and safe mine operation. The geospatial information construction using the existing total station has a disadvantage that a lot of work time is required because the target must be collimated and measured. In this study, the data of the mines were acquired with the total station and the 3D laser scanner, and the mine spatial information was constructed by using the shape based registration method. By using the static scanner data of some area applying the reference point surveying result of the total station, it was possible to construct the accurate result on the wide area acquired by the mobile scanner effectively. Also, the accuracy of the constructed geospatial information was evaluated and the deviation of mean 0.083m was shown. Point cloud products constructed through the research can contribute to the efficiency improvement of mine management by enabling quantitative analysis such as visualization of mine shape, distance, area and slope, and automation of drawing creation for cross section shape.