• Journal of Animal Science and Technology
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• 제63권2호
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• pp.262-271
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• 2021

Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice, rats, bats, and pigs; however, these two markers have not yet been described in the epididymis of bulls. Here, we examined the presence and distribution of the B1 subunit of V-ATPase (B1-VATPase) and KRT5 in the distinct regions of adult bovine epididymides, specifically, the caput, corpus, and cauda. Immunofluorescence staining and confocal microscopy showed that narrow shaped-clear cells were placed in the caput and corpus regions of the bovine epididymis; however, they were absent in the cauda epididymis. In addition, B1-VATPase was highly expressed in the cauda spermatozoa; however, it was rarely detected in the caput spermatozoa. On the other hand, KRT5-positive cells, basal cells, were maintained beneath the basal lamina and they had the traditional form with a dome-shaped morphology from the caput to cauda region of the bovine epididymis. The co-expression of B1-VATPase and KRT5 was confined to basal cells placed in the basal region of the epithelium. In summary, 1) clear cells were present with region-specific localization, 2) B1-VATPase was present in the corpus and cauda spermatozoa but absent in the caput, 3) co-expressed cells with B1-VATPase and KRT5 were present in the adult bovine epididymis, and 4) B1-VATPase was not a specific marker for clear cells in the bovine epididymis. Therefore, the perfect epididymal luminal condition created by the specific expression and localization patterns of B1-VATPase might be necessary to obtain fertilizing capacity of spermatozoa in the bovine epididymis.

• 한국항공우주학회지
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• 제50권5호
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• pp.309-316
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• 2022

본 논문에서는 14개의 다른 적층을 갖는 CFRP 적층판의 강도를 비선형 수치해석으로 계산하고 시험 결과와 비교하였다. 복합재 적층판 쿠폰은 일방향 테이프로 만들어진 프리프레그를 오토클레이브에서 경화하여 제작하였다. 라미나 시험으로 획득한 메트릭스의 비선형 특성을 해석에 고려하였다. Hashin 파손조건과 점진적 손상모델을 사용하여 비선형 유한요소 해석을 수행하였다. 비교 결과 본 논문의 접근 방법이 다양한 적층을 갖고, 손상이 없는 CFRP 적층판의 인장강도를 예측할 수 있음을 보여주었다. 그러나 구멍이 있는 적층판 시편의 강도 예측에는 부적합하였다.

• 대한정형외과학회지
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• 제56권4호
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• pp.351-356
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• 2021

부고환 결핵으로 부고환 절제술 및 항결핵제제를 복용중인 67세 남자 환자가 급성 요통 및 방사통을 주소로 내원하였다. 환자는 통풍의 과거력은 없었으나 혈액 검사상 고요산혈증 소견을 보였으며, 요추 후관절 및 후궁 부위의 골 파괴 병변이 관찰되었다. 요추 후관절 부위의 컴퓨터 단층촬영 유도하 바늘 생검을 실시하여 조직학적 검사를 시행한 결과 통풍성 척추관절병증 및 결핵성 척추염이 진단되었다. 환자는 통풍성 관절염에 대한 보존적 치료를 통해 증상은 호전되었다. 항결핵제제 복용 등 고요산혈증 위험을 가진 환자가 급성 요통을 호소하는 경우 통풍성 척추관절병증을 감별진단해야 하겠다.

• 대한소아치과학회지
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• 제49권3호
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• pp.348-356
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• 2022

과잉치는 치판의 과도한 성장과 분화에 의해서 발생된다. 이로 인해 이소맹출, 맹출지연, 인접치의 치근 흡수, 그리고 치간이개 등의 문제가 발생할 수 있다. 영유아기에서의 과잉치는 드물며 국내에서는 거의 보고되지 않았다. 이 증례에서는 3개의 매복된 과잉치가 있는 생후 2일된 신생아에 대해 보고하고 있으며, 환자는 21개월간 추적 관찰되었다. 유치열기에 맹출된 하나의 과잉치는 14개월이 되었을 때 중등도 진정 하에 발치되었다. 발치된 과잉치에 대해 micro-computed tomography (CT) 분석을 진행하여 치근단 부위의 미세한 형태 이상을 확인하였다. 과잉치 발치 후 치간이개는 감소하였고 구강위생도 개선되었다. 조기 진단 및 치료를 통해 과잉치로 인한 합병증을 예방할 수 있다.

• Journal of Veterinary Science
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• 제21권5호
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• pp.78.1-78.15
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• 2020

Background: Enteritis is one of the most frequently reported symptoms in piglets infected with porcine circovirus type 2 (PCV2), but the immunopathogenesis has not been reported. Objectives: This study examined the effect of a PCV2 infection on the intestinal mucosal immune function through morphological observations and immune-related molecular detection. Methods: Morphological changes within the ileum of piglets during a PCV2 infection were observed. The expression of the related-molecules was analyzed using a gene chip. The immunocyte subsets were analyzed by flow cytometry. The secretory immunoglobulin A (SIgA) content was analyzed by enzyme-linked immunosorbent assay. Results: The PCV2 infection caused ileal villus damage, intestinal epithelial cells exfoliation, and an increase in lymphocytes in the lamina propria at 21 days post-infection. Differentially expressed genes occurred in the defense response, inflammatory response, and the complement and coagulation cascade reactions. Most of them were downregulated significantly at the induction site and upregulated at the effector site. The genes associated with SIgA production were downregulated significantly at the induction site. In contrast, the expression of the Toll-like receptor-related genes was upregulated significantly at the effector site. The frequencies of dendritic cells, B cells, and CD8⁺T cells were upregulated at the 2 sites. The SIgA content decreased significantly in the ileal mucosa. Conclusions: PCV2 infections can cause damage to the ileum that is associated with changes in immune-related gene expression, immune-related cell subsets, and SIgA production. These findings elucidated the molecular changes in the ileum after a PCV2 infection from the perspective of intestinal mucosal immunity, which provides insights into a further study for PCV2-induced enteritis.

• 한국임상수의학회지
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• 제39권6호
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• pp.294-301
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• 2022

Three-dimensional (3D) printing technique has been widely used for accurate screw and pin placement in orthopedic surgery and neurosurgery. However, there are few reports comparing the accuracy between the patient-specific guides and freehand Kirschner wire (K-wire) placement in toy, small and medium breed dogs. This study aimed to assess the accuracy of 3D printed patient-specific guides (PSGs) in pin insertion in the thoracolumbar vertebrae of toy breed dogs and compare the outcomes between novice and experienced surgeons. The experiment was conducted on the thoracolumbar vertebrae of 21 euthanized toy breed dogs (median weight, 5.95 kg). The optimal insertion angle placement was determined and patient-specific guides for K-wire insertion were designed and 3D printed using computed tomography (CT) and a 3D computer-aided design program of three vertebrae (Thoracic 12-Lumbar 1). K-wire tracts were made by experienced and novice surgeons and compared to assess the accuracy based on postoperative CT. Based on postoperative CT, in the experienced group, 61 out of 63 pins (96.8%) were fully contained inside the vertebral body and lamina, whereas two pins (3.2%) had perforated the vertebral canal (grade 3, 2-4 mm breach). However, all the pins in the novice group were fully contained. The use of 3D printed PSGs for pin insertion in the thoracolumbar region is an accurate and safe alternative to freehand screw placement by novice surgeons in toy, small and medium breed dogs. Operations with 3D printed PSGs allow novice surgeons to achieve better or similar outcomes in accurate placement of pin/screws in vertebrae.

• Composites Research
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• 제22권6호
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• pp.32-38
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• 2009

본 연구에서는 탄소, 케블라 및 탄소-케블라 하이브리드 등 4가지 소재로 제작된 원형튜브시편에 대한 에너지 흡수능력 및 파손모드를 평가하였다. 이를 위해, 본 연구에서 일방향 프리프레그를 이용해서 원형튜브 제작하고 10mm/min의 하중속도로 준정적 압축시험을 수행하였다. 시험을 통해 취성파괴모드로 압축되는 탄소/에폭시로 제작된 튜브가 가장 우수한 에너지 흡수 특성을 보인 반면, 좌굴에 의해 압축되는 케블라/에폭시 튜브가 가장 낮은 에너지 흡수특성을 보였다. 하이브리드 [$90_C/0_K$]튜브의 경우 국부좌굴모드에 의해 에너지를 흡수했으며 우수한 압축후 구조온전성 특성을 보였다. [$90_K/0_C$] 튜브의 경우 주 파손모드는 단층굽힘모드이고 압축후 구조온전성이 확보되지 못했다.

• 한국환경복원기술학회지
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• 제26권5호
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• pp.47-56
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• 2023

The Quercus variabilis and Quercus dentata, which are said to be relatively drought tolerant among the important genus Quercus that represent deciduous broad-leaved forests in Korea. These two species are widely distributed worldwide in Korea, Japan and China (northern, central, western and eastern subtropical regions). This study compared the ecological niche breadth and overlap according to growth response in 4 soil moisture gradients for the two species and tried to reveal degree of competition and ecological niche characteristics. The ecological niche breadth was 0.977±0.020 for Q. variabilis and 0.979±0.014 for Q. dentata, the latter being slightly wider. And they were similar in 5 traits (stem length, leaf lamina length, leaf width length, stem weight, leaf petiole weight), Q. variabilis was more dominant in 4 traits (leaves number, stem diameter, leaf area, leaf petiole length), and Q. dentata was more dominant in 7 traits (root length, shoot length, plant weight, root weight, shoot weight, leaf weight, leaf petiole weight). The ecological niche overlap for soil moisture between the two species overlapped most in plant structure-related traits and least in photosynthetic organ-related traits such as petiole length. As a result of principal component analysis, degree of competition between the two species for soil moisture was more severe when the soil moisture condition was low than high. Among the measured traits that affect the two-dimensional distribution, 8 traits (Leaves number, Shoot length, Stem length, Plant weight, Root weight, Shoot weight, Stem weight, Leaves weight) were correlated with the factor 1, and 2 traits (Leaf width length, Leaf petiole weight) were correlated with the factor 2 (r>0.5). These results show that the ecological response of the two species to soil moisture is not a few traits involved, but several traits are involved simultaneously.

• Structural Engineering and Mechanics
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• 제88권2호
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• pp.169-178
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• 2023

Lightweight aggregate concrete (LWAC) has various advantages, but it has limitations in ensuring sufficient ductility as structural members such as reinforced concrete (RC) columns due to its low confinement effect of core concrete. In particular, the confinement effect significantly decreases as the axial load increases, but studies on evaluating the ductility of RC columns at high axial loads are very limited. Therefore, this study examined the effects of concrete unit weight on the seismic performance of RC columns subjected to constant axial loads applied with different values for each specimen. The column specimens were classified into all-lightweight aggregate concrete (ALWAC), sand-lightweight aggregate concrete (SLWAC), and normal-weight concrete (NWC). The amount of transverse reinforcement was specified for all the columns to satisfy twice the minimum amount specified in the ACI 318-19 provision. Test results showed that the normalized moment capacity of the columns decreased slightly with the concrete unit weight, whereas the moment capacity of LWAC columns could be conservatively estimated based on the procedure stipulated in ACI 318-19 using an equivalent rectangular stress block. Additionally, by applying the section lamina method, the axial load level corresponding to the balanced failure decreased with the concrete unit weight. The ductility of the columns also decreased with the concrete unit weight, indicating a higher level of decline under a higher axial load level. Thus, the LWAC columns required more transverse reinforcement than their counterpart NWC columns to achieve the same ductility level. Ultimately, in order to achieve high ductility in LWAC columns subjected to an axial load of 0.5, it is recommended to design the transverse reinforcement with twice the minimum amount specified in the ACI 318-19 provision.

• 대한영상의학회지
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• 제83권5호
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• pp.1153-1159
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• 2022

긴머리확장증은 확장, 연장 및 구불구불한 형태의 대뇌동맥을 특징으로 하는 드문 질환이다. 주요 병리기전은 내탄력판의 파괴로 알려져 있으며, 위험요인으로 고령, 만성 고혈압, 그리고 대사성 질환 등이 있다. 긴머리확장증은 주로 후순환계의 척추뇌기저동맥을 침범하는 것으로 알려져 있으나, 전순환계, 특히 전대뇌동맥에 이환되는 경우도 있다. 긴머리확장증이 전순환계와 후순환계를 모두 침범한 사례는 아직 국내에 보고된 바가 없다. 이에 우리는 기저 질환이 없는 젊은 여자 환자에서 전순환계와 후순환계가 모두 이환된 매우 희귀한 형태의 긴머리확장증 사례를 현저한 영상 소견을 토대로 보고하고자 한다.