• Journal of Environmental Science International
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• v.18 no.11
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• pp.1283-1289
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• 2009

Eco-physiological research and the control of Cochlodinium polykrikoides was carried out to elucidate eco-physiological characteristics of red tide organism through culture experiment depending on the condition of photon irradiance. Oxygen consumption of C. polykrikoides was high with a value of 1.12 mg/L/hr in the dark compared with that of 0.13 mg/L/hr at $100{\mu}mEm^{-2}s^{-1}$. DO values in a circular chamber with the lapse of time in seawater containing C. polykrikoides were declined in the dark period. DO values of seawater containing C. polykrikoides in the dark were declined from 7.01 mg/L to 2.65 mg/L in 30 cm depth and from 7.01 mg/L to 6.63 mg/L in 5 cm depth depending on the depth of circular culture vessel. Olive flounder, Paralichthys olivaceus and file fish, Stephanolepis cirrhifer exposed to Cochlodinium showed the separation of the lamella epithelium from gill filament, which disrupted the respiratory process at the gill level.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.4
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• pp.265-271
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• 1976

Parasitic protozoans of fish, such as Chilodonella, Costia, Trichodina, Ichthyophthirius are effectively eradicated by treating with a mixture of $50\~60\;ppm$ formalin, 0.2 ppm malachite green and 0.6 ppm dipterex in water recycling aquariums. In this case any histopathological changes in the gill of fish were not observed. The parasites began to disappear 6 hours after treatment. Upon increased concentrations of formalin of 130 to 140 ppm, a significant change on the epithelium of gills was observed in $24\~48\;hours $ after treatment. In this case gill epithelium degenerated and necrosis of the gill lamella supporting cells follows.

• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.161-166
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• 2018

The olfactory organ of Korean endemic fish Microphysogobio yaluensis are described anatomically and histologically, focused on relationship to its habitat and ecology. The paired olfactory organs are located at the dorsal snout, and externally consist of two semicircular nostrils and single nasal flap. They internally have rosette structure with 22 to 24 units of lamellae and the raphe inside the olfactory chamber. The lamella is made up of the sensory and the non-sensory epitheliums. The sensory epithelium has olfactory receptor neurons, supporting cells and basal cells whereas the nonsensory epithelium has stratified epithelial cells, ciliated non-sensory cells and mucous cells with acidic and neutral mucins. These structures might be considered that M. yaluensis has the olfactory organ which corresponds to the high sensitivity for its habitat and ecology, and is usable as a taxonomic key.

• Applied Microscopy
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• v.30 no.3
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• pp.311-319
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• 2000

The gill morphology and ultrastructure of the fleshy shrimp, Penaeus chinensis were investigated by light and electron microscopy. Fleshy shrimp has dendrobranchiate gills. Gill has a longitudinal septum dividing them into afferent and efferent channel. Each gill lamella is covered by multi-layered thin cuticle of different electron density. The lamella basal cell is squamous and contains cytoplasm of electron dense. Simple epithelial layer consists of squamous epithelium contained large nucleus. The lamella pillar structures are characterized by the axial microtubules and lateral membrane interdigitations Secretory cells of AB-PAS negative are multicellular gland. In active gland each cell boundary is not apparent and the cytoplasm contains smooth endoplasmic reticula, mitochondria, membrane-bounded secretory vesicles of low electron density and granular resettes. In inactive gland each cell boundary is apparent and the cytoplasm is occupied with numerous small granules of electron dense. The well-developed rough endoplasmic reticula and Golgi apparatus are observed in the unicellular gland of alcian blue positive.

• Applied Microscopy
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• v.30 no.1
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• pp.101-111
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• 2000

In this study, the distribution of lectin receptors in Paragonimus westermani tissue was explored using colloidal gold label complexed with lectin WGA purified from wheat germ (Triticum vulgare). The lectin WGA gold complex, shown to recognize GlcNAc (N-acetylgalactosamine) and NeuNAc (N-acetylneuraminic acid) regions, was applied to detect binding sites in Lowicryl HM 20 sections viewed under electron microscope. Labeled sections of the metacercaria revealed gold particles specifically distributed on the tegumental syncytium and lamella of the excretory canal. Labeling of young adult tissue was then quantified and compared to that of adult worm tissue. Adult worm tissue sections resulted in specific gold particle distribution on the lamella of caecal epithelium and excretory canal. These results indicate that lectin WGA receptors are located in the tegumental syncytium and lamella of the excretory canal of the metacercariae, and in the lamella of the caecum and excretory canal of the young adult and adult. Therefore, the GlcNAc and NeuNAc regions in the tegumental syncytium appear to be functionally associated with cell-recognition and protection from the immune system of the host, and linked with membrane transport and absorption of nutrients in the lamella of the excretaory canal and caecal epithelia.

• Journal of fish pathology
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• v.9 no.2
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• pp.119-126
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• 1996

We report on the variation of infection and histopathological change of Microcotyle sebastisci parasitic on cultured rockfish, Sebastes schlegeli, in Namhae Islands and Kamak Bay from April to October in 1995. Microcotylosis due to Microcotyle sebastisci occurred among cultured rockfish, Sebastes schlegeli. This parasite was not found on the host fish from June to July. Parasite sites were mainly consist of 2nd and 3rd gill arch`s filaments of rockfish. Also, the sites were secreted in large quantity of mucus with a very small bleeding. In Namhae Islands, maximum values of prevalence, relative density and mean intensity were found on September 1995, as 40.0%, 30.7 and 76.8, respectively. In Kamak Bay, maximum values of prevalence, relative density and mean intensity were obtained on October 1995, as 46.0%, 40.5 and 88.0, respectively. Histopathological changes of the heavily infested gills were showed necrosis, epithelium of the gill filaments underwent hyperplasia with fusion of the lamella and filamental clubbing. And a bacterial colony is invaded on the surface of lamella epithelium.

• Korean Journal of Ichthyology
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• v.34 no.3
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• pp.153-159
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• 2022

The olfactory organ of a Korean endemic species, Pseudobagrus koreanus, was investigated to describe its morphology and histology and analyze correlation between habitat environment and its ecological habits and characters, using a stereo microscope, a light microscope, and a scanning electron microscope. Its external morphology showed tubular anterior nostril at the tip of the snout and slit posterior nostril at the base of the nasal barbel. The olfactory chamber showed the rosette structure consisting of numerous lamellae radial and parallel to the medium raphe. The olfactory lamella has the sensory epithelium with olfactory receptor nuerons, supporting cells, basal cells, lymphatic cells, plasma cells and the non-sensory epithelium with stratified epithelial cells, mucous cells, lymphatic cells. In particular, 1) 41~43 (n=20) lamellae, 2) lymphatic cells and plasma cells, 3) mucous cells including neutral polysaccharid may be olfactory characteristics relevant to a stagnant or a standing habitat environment, nocturnal habit dependent on olfaction, and taxonomic characters compared at least to other catfish species.

• Applied Microscopy
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• v.34 no.2
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• pp.139-143
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• 2004

The ultrastructure of the gills of Oplegnathus fasciatus was examined by means of light and transmission electron microscopes. The gills have primary and secondary filaments (lamellae). The following cells are identified and described : epithelial cell, pillar cell, chloride cell and mucose cell. The simple epithelial layer consists of squamous epithelium containing a large nucleus and the surface is covered with some of microridges. The lamella pillar structures are characterized by axial microtubules and lateral membrane interdigitations. Chloride cells contain a lot of mitochondria and specifically developed tubular systems. The rough endoplasimic reticulum and golgi complex, and some of mucous granules were observed in immature mucous cells. The mature mucous cells were AB-PAS positive, globular in shape, and had mucous granules of similar size with various electron densities.

• Applied Microscopy
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• v.39 no.4
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• pp.319-324
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• 2009

Morphology of the gill in Lateolabrax japonicus was investigated after staining the gill, as a result, we found the gill is composed of gill raker, gill arch, gill filament and gill lamellae. The number of gill raker was 7~10 in the upper and 13~18 in the lower. Ultrastructure of the gill in Lateolabrax japonicus were examined by means of the light and transmission electron microscopes. The gill have primary filament and secondary filament (lamellae). The following cells are identified and described: pavement cell, pillar cell, blood cell, mucose cell and chloride cell etc. Simple epithelial layer consists of squamous epithelium contained large nucleus, intracellular organelles etc. and the surface is covered with some of microridges. The lamella pillar structures are characterized by the axial microtubules and lateral membrane interdigitations. The mucous cells were globular in shape, and had almost the mucous granules of same size with various electron density. Chloride cells contain a lot of mitochondria and specifically developed tubular systems.

• Journal of fish pathology
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• v.6 no.1
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• pp.65-70
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• 1993

The delicate histological structure of gill in teleosts can be easily affected by a variety of biological, chemical or physical detrimental agents because it is directly exposed to the surrounding water. The epithelium of secondary lamella is thin to allow efficient gaseous exchange and this also renders it particularly vulnerable to various pathogens. As well as the main respiratory role, the gill has other various important functions such as acid-base balance, osmoregulation or the excretion of nitrogenous waste products. Thus destruction of epithelial integrity such as epithelial necrosis or thickening can render a fish very vulnerable to respiratory, secretory and excretory difficulties. This article was tried to describe in detail the common processes of pathological responses correlated to the normal histological structures of the gill in teleosts.