• Current Research on Agriculture and Life Sciences
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• 제17권
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• pp.59-63
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• 1999

벼의 엽록체 small HSP의 고온 스트레스 하에서의 기능을 밝히기 위하여 Oshsp21 cDNA를 pET 발현 vector에 도입하였다. 형질전환된 대장균 배양액에 IPTG를 첨가하여 단백질 발현을 유도시킨 다음 고온 stress 하에서의 생존율을 대조구와 비교하였다. 그 결과 대조균주의 경우 $50^{\circ}C$에서의 생존율이 크게 감소하였으나 Oshsp21이 발현된 대장균의 경우 70% 이상의 생존율을 나타내었다. 또한 대장균 단백질을 $55^{\circ}C$에서 30분간 열처리한 후, 대장균 단백질을 가용성과 비가용성 단백질로 분획한 다음, 각각의 비율을 조사한 결과, 대조균주의 경우 총 단백질의 약 60%가 비가용성 단백질로 변성되었으나, Oshsp21을 발현시킨 대장균의 경우 총 단백질의 약 35%만이 비가용성 단백질로 나타났다. 이러한 결과는 벼의 엽록체 small HSP는 세포내에서 분자 chaperone으로 기능하여 고온내성을 부여할 수 있음을 나타낸다.

• 동의신경정신과학회지
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• 제16권1호
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• pp.129-142
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• 2005

Objectives : This study was to investigate MMPI profile on clinical scales and personality scales of Panic Disorder patients. Methods : Thirty eight Panic Disorder patients and twenty eight normal control were administered the MMPI. Experimental design was done by nonequivalent control group and statistics were crosstabs, chi-square test, ANOVA, t-test and cluster analysis Results and Conclusions : 1. The MMPI clinical scales profile of Panic Disorder patients was neurotic profile of 2-7 type. Panic Disorder patients was elevated the other scales except L, K, Mf and Ma scales for control group. 2. A result of grouping MMPI in Panic Disorder patients, we could classify into 3 different groups. Group 1 is a profile of conversional neurosis. Group 2 is a normal proflie. Group 3 is a proflie of $'{\Lambda}'$ type neurosis. 3. Personality characteristics in Panic Disorder patients was lowed scores in HST, NAR scales, elevated the other scales except ANT scale. Specially in PAG, AVD scales showed high scores.

• Journal of Veterinary Science
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• 제22권3호
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• pp.39.18-39.18
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• 2021

Background: Interferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear. Objectives: The purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV). Methods: The effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods. Results: In this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV. Conclusion: Expression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV.

• Korea-Australia Rheology Journal
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• 제20권2호
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• pp.89-94
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• 2008

To satisfy good mechanical and optical properties of polymer-coated film products, it will be indispensable to elucidate the molecular orientation of polymer chains within coating liquids in coating flows. Using hybridized numerical method between computational fluid dynamics (CFD) and Brownian dynamics (BD) simulations can provide the useful information for the better quality control of coated films. Flexible polymer chains, e.g., ${\lambda}$-DNA molecules here, change their conformation according to the flow strength and the flow type. The molecular conformation within the coated film on the web or substrate is quite different, because the polymer chains experience the complicated flow strength and flow types in flow field. Especially in the slot coating flow, these chains are more extended by the extension-like flow field generated in the free surface curvature just beyond the downstream die region. Also, the polymer chain extension beneath the free surface can be affected by the die geometry, e.g., the coating gap, changing flow field.

• 말소리와 음성과학
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• 제1권4호
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• pp.177-185
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• 2009

This study investigates the differences in mean F0, intensity, jitter and shimmer across hearing aid, gender and vowels. For this study, 20 hearing-impaired adults and 20 normal hearing adults as a control group were asked to read 7 Korean vowels(/$\alpha$, $\Lambda$, o, u, ɯ, i, $\varepsilon$/). Subjects' readings were recorded by NasalView and analyzed by Praat. Results showed that the means of F0 were significantly higher in the hearing impaired group(HL) than in the normal hearing group(NH), in the female group than in male group, and in high vowels than in low vowels. Second, intensity was significantly higher in the normal hearing group(NH) than in the hearing impaired group(HL), in male group than in female group, and in low vowels than in high vowels. Third, jitter was significantly higher in the normal hearing group(NH) than in the hearing impaired group(HL), and in female group than in male group and in the back vowels than in front vowels. Finally, shimmer was significantly higher in the normal hearing group(NH) than in the hearing impaired group(HL), and in male group than in female group. In particular, the male group showed that front vowels tend to have higher shimmer than back vowels.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2004년도 춘계 학술대회논문집
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• pp.126-131
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• 2004

The interaction between the hypersonic free stream and the side jet flow at high altitudes is investigated by direct simulation Monte Carlo(DSMC) method. Since there is a great difference in density between the free stream and the side jet flow, the weighting factor technique which could control the number of simulation particles, is applied to calculate these two flows simultaneously. Chemical reactions are not considered in the calculation. For validation, the corner flow passing between a pair of plates that are perpendicularly attached is solved. The side jet flow is then injected into this comer flow and solution is found for the merged flow. Results are compared with the experiments. For a more realistic rocket model, the flow past a blunted cone cylinder shape is solved. The leeward or windward jet injection is merged with this flow. The effect on the rocket surface is observed at various flow angles. The lambda effect and the wake structure are found like low attitudes. High interaction between the free stream and the side jet flow is observed when the side jet is injected in the windward direction.

• 한국가시화정보학회지
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• 제7권2호
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• pp.64-71
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• 2010

The flow and noise characteristics of wake behind wind-turbine blades have been investigated experimentally using a two-frame particle image velocimetry (PIV) technique. Experiments were carried out in a POSTECH subsonic large wind-tunnel ($1.8^W{\times}1.5^H{\times}4.3^L\;m^3$) with KBP-750D (3-blade type) wind-turbine model at a freestream velocity of $U_o\;=\;15\;m/s$ and a tip speed ratio $\lambda\;=\;6.14$ (2933 rpm). The wind-turbine blades are connected to an AC servo motor, brake, encoder and torque meter to control the rotational speed and to extract a synchronization signal for PIV measurements. The wake flow was measured at four azimuth angles ($\phi\;=\;0^{\circ}$, $30^{\circ}$, $60^{\circ}$ and $90^{\circ}$) of the wind-turbine blade. The dominant flow structure of the wake is large-scale tip vortices. The turbulent statistics such as turbulent intensity are weakened as the flow goes downstream due to turbulent dissipation. The dominant peak frequency of the noise signal is identical to the rotation frequency of blades. The noise seems to be mainly induced by the tip vortices.

• 한국미생물·생명공학회지
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• 제19권3호
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• pp.228-234
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• 1991

DNA 자동합성기를 이용하여 오릴고뉴클레오타이드들을 합성하였으며 이로부터 인간 인터루킨-2를 코드하는 유전자를 제조하였다. 합성 유전자의 염기서열은 대장균에서 사용빈도가 높은 코돈을 고려하여 결정하였으며, 이때 인터루킨-2의 아미노산 서열은 변화시키지 않았다. 합성된 유전자는 람다 피엘 프로모터를 사용하여 대장균에서 발현시켰다. 인터루킨-2는 대장균에서 불용성의 침전물로 생성되었다. 생성된 인터루킨-2는 인터루킨-2 요구 배양세포주의 성장을 촉진하였다.

• 미생물학회지
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• 제26권4호
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• pp.291-297
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• 1988

신호배열 돌연변이인 rbsB 103는 전구체 리보스 결합단백질을 세포질내에 축적시키고, rbsB 106 복귀유전자는 이 전구체를 숙성 가능하게 하여 페리플라슴으로 수송되게 한다.(Iida el at., 1985, Park, el at., 1988). 본고에서는 rbsB 유전자의 세 allele, rbsB, rbsB 103와 rbsB 106를 이들이 코딩하는 단백질을 대량생산 하그l자 람다 P 프로모터 조절하에 클론하고 나아가서 다섯종의 단백질을 순수정제하였다.. 전구체 단백질의 pI는 8.0, 숙성단백질의 $P_{L}$는 7.5임을 밝혔다. 순수정제된 단백질의 아마노 말단의 아미노산 배열을 결정하여 DNA 염기서열로부터 밝혀진 아미노산 변화를 확인하였다.

• 한국정보통신학회논문지
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• 제2권1호
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• pp.71-77
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• 1998

본 논문에서는 LMS와 CMA 알고리즘에 의한 적응 배열안테나의 training 원리와 제어방법을 기술하고 수렴특성, 지향성 패턴의 적응성, SINR 및 신호파의 재현 특성을 비교 분석한다. LMS와 CMA 적응 안테나 원리를 적용한 선형 $\lambda$/4 간격 4소자 배열안테나에 $\pi$/4 QPSK 신호파를 인가했을때 정상상태에서 SINR가 각각 13.8［dB］와 12.8［dB］로써 CMA에 비하여 LMS가 우수한 SINR와 빠른 수렴특성을 보였으며 간섭파의 방향에 적응하여 강한 영점을 잘 형성하는 것으로 나타났다.