• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.267-274
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• 2010

The objective of this experiment was to investigate effects of total mixed ration (TMR) of different nutrition level on milk production in dairy goats (Sannen). Twenty four lactating goats were randomly assigned to one of four TMRs; medium energy-medium crude protein (CP) TMR (control), high energymedium CP TMR (T1), medium energy-high CP TMR (T2) and high energy-high CP TMR (T3). The content of total digestible nutrients (TDN) and CP in the control diet were 67.0% and 13.9%. The TDN content of the high energy TMR was 73.7% and the CP content of the high CP TMR was 15.3%. Milk yield was significantly (P<0.05) higher for T1 ($2,198{\pm}115 ml$) than for T2 ($1,742{\pm}52 ml$) and T3 ($1,984{\pm}90 ml$). But there were no significant differences in milk composition between the treatments. Daily body weight gain was increased in T1 and T3 but reduced in control and T2. The result of the present study showed that dietary energy level supplied more than that of NRC recommendation did not affect milk yield of dairy goats in early lactation but prevented body weight loss.

• Korean Journal of Organic Agriculture
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• v.27 no.2
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• pp.147-160
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• 2019

This study was conducted to evaluate roughage to concentrate ratio on the changes of productivity and metabolic profiling in milk. Six lactating Holstein cows were divided into two groups, T1 group was fed low-concentrate diet (Italian ryegrass to concentrate ratio = 8:2) and T2 group was fed high-concentrate diet (Italian ryegrass to concentrate ratio = 2:8). Milk samples were collected and its components and metabolites were analyzed by ¹H-NMR (Nuclear magnetic resonance). The result of milk components such as milk fat, milk protein, solids-not-fat, lactose and somatic cell count were not significantly different between two groups. In carbohydrate metabolites, trehalose and xylose were significantly higher (P<0.05) in T1 group, however lactose was not significantly different between two groups. In amino acid metabolites, glycine was the highest concentration however, there was no significant difference observed between two groups. Urea and methionine were significantly higher (P<0.05) in the T2 group. In lipid metabolites, carnitine, choline and O-acetylcarnitine there were no significant difference observed between the two groups. In benzoic acid metabolites, tartrate was significantly higher (P<0.05) in T2 group. In organic acid metabolites, acetate was significantly higher (P<0.05) in T1 group and fumarate was significantly higher (P<0.05) in T2 group. In the other metabolites, 3-methylxanthine was only significantly higher (P<0.05) in T2 group and riboflavin was only significantly higher (P<0.05) in T1 group. As a result, milk components were not significantly different between two groups. However, metabolites concentration in the milk was significantly different depends on roughage to concentrate ratio.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.131-142
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• 2003

We evaluated the effects of adding fibrolytic enzyme into ruminant diets on ruminal fermentation (in vitro) and lactational performances of dairy cows (in vivo). Through the in vitro experiment that was carried out with different contents of NDF (34, 38, 43%) in diets, digestibilities of NDF in the rumen appeared not significantly different by the addition of enzyme but were different by NDF content in diets showing higher digestibility in NDF 43% diet. It could be attributed by the relatively higher amount of hemicellulose in the current experimental diets than in conventional diets that might have been digested easily by the addition of fibrolytic enzyme in the rumen. The addition of fibrolytic enzyme tended to increase NDF digestibilities to a little extent both in 0.05 and 0.1% enzyme levels. Ruminal pH, NH3-N concentrations and VFA production in the rumen were not affected by the addition of fibrolytic enzyme. Activities of CMCase and xylanase were higher in enzyme treated diets of both NDF 34 and 38%. In particular, the activities of xylanase that slowly decreased from 0 to 12 hr but rapidly after 24 hr indicates that the major action of the enzyme in the rumen occurs in early period of incubation. Through an in vivo experiment, fibrolytic enzyme addition into the diets of dairy cows indeed affected lactational performance of milk yield. The cows fed enzyme treated diets produced 8% (1.9kg/d) more amounts of milk than with no enzyme addition. Milk composition of milk fat and protein was not affected by enzyme addition. Overall, the results of this in vivo study indicates that fibrolytic enzyme can be used to improve milk production in lactating cows. In respect that animals in different treatments of this study had the same amounts of intake, the increased milk yield with enzyme addition may be attributed to the improved utilization of nutrients in the digestive tract.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.703-710
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• 2003

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of $\beta$-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F$_2$ population composed of 214 individuals from an intercross between Korean Native Boars and Landrace sows. PCR products from two primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and HinfⅠ, revealed fragment length polymorphisms(RFLPs). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP/HinfⅠ was .38, .41 and .20, respectively, in the population. Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weights at 3, 5, 12, and 30 week of age (p〈.05 to .001). The ‘d’ allele was associated with gaining of body weight. In H-FABP/HinfⅠ locus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p〈.05 or p〈.001) and back fat thickness, body fat including abdominal and trimmed fat (p〈.001) and intramuscular fat(p〈.05) The ‘H’ allele was positively associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.265-272
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• 2009

A total of 486 milk records were collected from 16 diary farms in Imsil-gun, Jeollabuk-do. Results obtained were as follows: The average 3MG (amount of milk within the first three minute) was 7.44 kg and 55% of total milk yield was produced within 3 min. The average of SPL (% of foam in milk) was 33.93% and the average of MNG (strip yield) was 0.14 kg, which was less than 1% of total milk yield. The averages of HMF (highest milk flow), HMG (maximum milk flow rate in one minute) and DMHG (average milk flow in the main milking phase) were 3.03 kg/min, 2.94 kg/min and 2.05 kg/min, respectively and the average milking speed in Imsil-gun was slower than other regions. The average of tS500(time to reach 0.5 kg/min at beginning) was 0.23min (about 14 seconds) and that of tMGG (duration of the total milking) was 7.75min. The average tMBG (duration of the dry milking phase) was 0.58 min (35 seconds) and that of tMNG (duration of the stripping phase) was 0.42min (14 seconds). The averages of ELHMF (electrical conductivity at highest milk flow) and ELAP (beginning peak level of the electrical conductivity) were 6.81 mS/cm and 7.58 mS/cm, respectively. The average of ELMAX (maximum electrical conductivity) was 7.48 mS/cm and that of ELAD (beginning peak difference of the electrical conductivity) was 0.61 mS/cm. While the total milk yields for DMHG, tMHG (duration of the main milking phase), tPL (duration of the plateau phase), tAB (duration of the descending phase) and tMGG were positively correlated (0.35~0.54), those for tMBG and SPL were negatively correlated (-0.11 and -0.27). As the DMHG increased, tMHG, tPL, tAB, tMGG and SPL decreased. While the cows with higher electrical conductivity at the beginning of milking had less somatic cell counts, cows with higher electrical conductivity after the peak of milk yield had more somatic cell counts. The results of this experiment indicated that through milking based on milking and lactating standards and the regular checking of milking status, the qualities of milk and milk yields could be improved.

• Journal of Animal Environmental Science
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• v.2 no.1
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• pp.41-52
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• 1996

This experiment was carried out to study the effect of sprinkler and fan cooling system on the physiological parameter, milk production and milk composition for Holstein cows in hot, humid climates. Thirty cows were assigned to one of two sections of open-sided loose barn. Water nozzles of sprinkler system were spaced in line at 1.2m intervals. Forced air was provided by 85cm diameter fans at rate of 3.4㎥/sec. The results obtained from these experiments are as follows: 1. There was no significant difference in meteorological data between control and fan + sprinkler cooling system(treatment group). 2. Skin temperature and rectal temperature of the treatment group were significantly lower than those of the control group (32.96 : 39.53$^{\circ}C$ vs 34.02 : 41.21$^{\circ}C$ respectively) (P<0.05). 3. Serum cortisol concentration of the treatment group(0.90$\mu\textrm{g}$/dL) was lower than that of control group(1.44$\mu\textrm{g}$/dL)(P<0.05). 4. Milk production of cows cooled with a sprinkler and fan cooling system was significantly higher than that of no cooling system (P<0.0l). 5. Lactose, protein and solid-not-fat content of milk were not changed by the treatments. Milk fat content of the control(3.23%) was low compared with the treatment group(3.38%). Somatic cell count was reduced by 26.63% in the treatment. The results indicate that a sprinkler and fan cooling systems can provide an effective means to relieve heat stress and enhance productivity of lactating Holstein cows during hot and humid summer season.

• Journal of Nutrition and Health
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• v.25 no.2
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• pp.123-131
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• 1992

Concentrations of 5 major minerals(Na, K, Ca, P, Mg) and 3 trace elements(Mn, Mo, Ni) were measured in human milk samples collected from 21 highly selected healty lactating women at 2-5 days and at 1, 2, 4, 6 and 12 weeks postpartum. Significant decrease in contents of the major minerals and trace elements were found with time postpartum. Sodium contents decreased from 293.01ug/N at 2-5 days to 194.27ug/N at 12 weeks. Potassium contents also decreased from 436.18ug/N in colostrum to 358.51ug/N in matured milk at 12 weeks. The Ca/P ratios of colostrum transitional and matured milk were 3.39, 281 and 2.45 respecti-vely. Mean manganese levels of colostrum and transitional were 0.024ug/N and those of matured milk were 0.014ug/N. Molybdenum concentrations in the breast-milk collected at 1, 2, 4 weeks were higher than those in the milk samples at 2-5 days and 12 weeks Nickel content of colostrum was 0.062ug/N and those of trnsitional matured milk were 0.22ug/N and 0.017ug/N, These determinations will provide the basic information on the variability of minerals and trace elements as lactation proceed and the comparison of the components between term and preterm milk.

• Journal of Korean Home Economics Education Association
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• v.35 no.1
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• pp.1-14
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• 2023

The objective of the study was to examine the associations of dietary carbohydrate and fat intake with the prevalence of metabolic syndrome in Korean women. A cross-sectional study was employed based on data from the Korea National Health and Nutrition Examination (2007-2016). A total of 22,850 women aged 19 to 69 years were studied after excluding responses from pregnant or lactating women and those with missing metabolic values. Dietary intake data were collected with a 24-hour recall method. Dietary carbohydrate and fat intakes were divided into quintiles. After controlling for confounding variables, a multivariable logistic regression and general linear model were used. The findings indicated that HDL cholesterol levels were lower (p for trend<0.01), while triglyceride levels (p for trend=0.04), waist circumference (p for trend<0.01), and systolic blood pressure (p for trend<0.01) were higher among participants in the highest quintile of carbohydrate intake compared to those in the lowest quintile. Participants in the highest quintile of fat intake had lower waist circumference (p for trend=0.02), triglyceride level (p for trend<0.01), and systolic blood pressure (p for trend<0.01), while higher HDL cholesterol level (p for trend<0.01) compared to those in the lowest fat intake quintile. Metabolic syndrome was more likely to be present in the highest quintile of carbohydrates intake than in the lowest quintile (5^th quintile vs. 1^st quintile, OR: 1.32; 95% CI: 1.11 to 1.57). However, metabolic syndrome was less likely to be present in the highest quintile of fat intake than in the lowest quintile (5^th quintile vs. 1^st quintile, OR: 0.73; 95% CI: 0.61 to 0.86). This study revealed that high dietary carbohydrate intake and low dietary fat intake were associated with metabolic syndrome in Korean women.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.373-386
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• 2004

This study was conducted to investigate effects of the brown seaweed residues supplementation on in vitro fermentation, and milk yield and milk composition of dairy cows. Therefore, two experiments consisting of an in vitro and an in vivo growth trial were used. In in vitro experiment, brown seaweed residues(BSR) was supplemented in basal diet with 0, 1, 2 and 4% respectively, and incubated for 3, 6, 9, 12, and 24 h. The pH value, ammonia-N and VFA were investigated. The pH value tended to increase with increasing BSR during the incubation. Particularly, pH was significantly higher in BSR treatments compared with control at 9 h(p < 0.05). While, ammonia-N concentration was not significantly different across treatments during the whole incubation. BSR supplementation did not affect total VFA production, but acetate was linearly increased in BSR treatments compared with control at 12 h(p < 0.05), and its concentration was highest(92.70 mM) in 4% BSR among treatments. The concentration of iso-butyrate tended to increase in BSR treatments in comparison to control during the incubation. In addition, the concentration of iso-valerate was higher in BSR treatments compared with control at 12 and 24 h. In growth trial, BSR was added(800 g/d/animaI) to diets of dairy cow. Dry matter intake was not affected by BSR supplementation, but daily milk yield(kg) significantly increased in BSR treatment compared with control(p < 0.05). However, milk composition(%) and milk yield(kg) were not significantly different between treatments. Milk fat(% and kg/d) tended to slightly decrease in BSR treatment compared with control(3.59% and 1.06 kg/d vs. 3.32% and 1.01 kg/d), The contents of C16:0 and C20:4 in milk significantly increased in BSR treatment compared with control reflecting from dietary fatty acid composition. The content of C18:0 in milk which is end product of biohydrogenation of CI8 unsaturated fatty acids in the rumen significantly increased in BSR treatment compared with control(p < 0.05). C18:2 content in milk tended to decrease, but tended to increase trans-II C18:l and CLA contents in milk in BSR treatment compared with control. In conclusion, it could be summarized that BSR may stabilize rumen pH, and it could improve milk yield and CIA content in milk with more than 4% of diet. Therefore, BSR could be beneficially used in dairy diets as a feed additive.

• Korean Journal of Agricultural Science
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• v.29 no.2
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• pp.20-34
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• 2002

This study was conducted to examine the effects of an Aspergillus oryzae fermentation culture on the in vitro digestibilities of dry matter, crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF), crude protein, and pH in in vitro experiment of diets for dairy cows. A fungal species, Aspergillus oryzae was supplied by Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea (KCTC 1229). The experimental diets were commercial compound feed (concentrate) and total mixed ration (TMR) for lactating cows, of which chemical analyses were determined at Research and Development Institute, Woosung Feed Co., Ltd., while the digestibilities were done at the laboratory of Chungnam National University. Aspergillus oryzae culture products were added to compound feed and TMR at the rate of 0, 1.0, 2.0, 3.0% respectively. The experimental diet with the rumen fluid sampled from Holstein fresian milking cows were used and digested for 24 hrs, 48hrs and 72hrs in the shaking incubator. The residues of the digesta were digested for 48hrs in the incubator in which put 30ml of 0.1N HCl with 0.2% pepsin at $39^{\circ}C$. The final precipitates were dried for 48hrs in the drier at $60^{\circ}C$. These experimental procedures were triplicated to determine the in vitro digestibility of dry matter, crude fiber, ADF, NDF, crude protein and pH. Compared to control diet, not added Aspergillus oryzae, the DM digestibility of fungal diets were improved 2.1%(63.1%), 9.7%(68.5%) and 9.0%(68.0%) for 24 hour fermentation in compound feed while 4.8%(60.0%), 6.4%(61.1%) and 2.9%(58.8%) in TMR. On the contrary, for 48 hour and 72 hour digestibilities, the effects of Aspergillus oryzae culture on the digestibility of dry matter were relatively lowered compared to 24 hour digestibility. Referring to the digestibility of dietary fiber, Aspergillus oryzae was believed to significantly improve digestibilities of crude fiber, ADF and NDF. Those were increased up to 13.3%(53.3%) for 24 hour fermentation, while 2.4%(54.6%) for 3.0% added for 72 hour fermentation in compound feed. However, there were no significant differences among the treatments for the inclusion rate of Aspergillus oryzae, even though the more inclusion rate, the better digestibility. The protein digestibilities were significantly improved from 0.4%(79.7%) to 9.4%(71.8%) by adding Aspergillus oryzae into compound feed. However, there were no significant differences between the two experimental diets, 2.0% and 3.0% Aspergillus oryzae included diets. In case of TMR, the protein digestibilities were significantly improved from 4.0%(70.4%) to 6.3%(65.1%) by adding Aspergillus oryzae. However, there were no significant differences between the two experimental diets, 2.0% and 3.0% Aspergillus oryzae included diets. In this study, there were no significant differences among the treatments in pH. On the contrary, there were slightly decrease in pH by adding Aspergillus oryzae into experimental diets but not significant. Summarizing the results of this examination, Aspergillus oryzae fermentation culture is believed to improve the digestibilities of dry matter, fiber and crude protein in cattle diets. However, more detailed research for the mechanism of the fungal culture is required to improve ruminal environment.