• Biomedical Science Letters
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• v.28 no.3
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• pp.206-210
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• 2022

Calystegia dahurica (Herb.) Choisy is a natural product that has not been studied for efficacy or active ingredients. The purpose of this study is to investigate the activation effect of natural killer cells using a natural extract composition based on Calystegia dahurica (Herb.) Choisy extract (CDCE). We evaluated the activity of natural killer cells in natural products using PBMCs from healthy participants. All natural products were extracted with 50% ethanol. Based on the results of the cell viability assay, PBMCs of healthy participants were treated with extracts at various concentrations. Then, analysis was performed using flow cytometry to measure the cd107a surface expression of natural killer cells. As a result, treatment with a single extract of PBMCs increased the expression of cd107a in a concentration-dependent. Furthermore, it was confirmed that the treatment of the extract composition showed the highest expression of cd107a. In conclusion, it is expected that the extract composition containing CDCE according to this study can be used for prevention or treatment of cancer cells, tumor cells, and immune diseases.

• Parasites, Hosts and Diseases
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• v.52 no.2
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• pp.163-168
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• 2014

Wnt proteins are a family of secreted glycoproteins that are evolutionarily conserved and considered to be involved in extensive developmental processes in metazoan organisms. The characterization of wnt genes may improve understanding the parasite's development. In the present study, a wnt4 gene encoding 491amino acids was amplified from cDNA of metacestodes of Taenia solium using reverse transcription PCR (RT-PCR). Bioinformatics tools were used for sequence analysis. The conserved domain of the wnt gene family was predicted. The expression profile of Wnt4 was investigated using real-time PCR. Wnt4 expression was found to be dramatically increased in scolex evaginated cysticerci when compared to invaginated cysticerci. In situ hybridization showed that wnt4 gene was distributed in the posterior end of the worm along the primary body axis in evaginated cysticerci. These findings indicated that wnt4 may take part in the process of cysticerci evagination and play a role in scolex/bladder development of cysticerci of T. solium.

• Journal of Microbiology and Biotechnology
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• v.29 no.8
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• pp.1281-1287
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• 2019

Nattokinase (NK, E.C. 3.4.21.62) is a serine protease produced by Bacillus subtilis natto that shows promise for the treatment of thrombotic disease. In this study, we assessed the effects of NK on the development of hepatocellular carcinoma (HCC), a principal malignancy of the liver that causes morbidity and mortality worldwide. Crude extracts of NK (NCE) were isolated from fermentation medium by centrifugation and separated into three fractions (<10 K, 100~30 K and >30K). Orthotopic HCC mouse models were established and NCE was administered by oral gavage. H&E staining was performed to examine the pathology of HCC livers. Immunohistochemistry and immunofluorescence were used to evaluate FOXM1, CD31, CD44 and vimentin expression in the liver. Compared to PBS groups, NCE increased the survival rates of HCC-bearing mice to 31% and decreased ascites. Low-intensity ultrasound imaging showed that the hypoechoic mass area was lower in NCE-treated mice and that tumor growth significantly decreased. IHC staining showed that the expression of FOXM1 was inhibited by NCE treatment. Immunofluorescence results revealed lower levels of CD31, CD44 and vimentin in the NCE groups. Taken together, these data demonstrate that NCE from Bacillus subtilis natto improves survival and inhibits tumor growth in HCC mice.

• Journal of Veterinary Science
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• v.23 no.5
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• pp.32.1-32.11
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• 2022

Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

• Journal of The Korean Association For Science Education
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• v.4 no.1
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• pp.15-25
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• 1984

The purpose of this study is to develop a practical and ideal model of science laboratory enrolled 48-60 students by studying optimal facilities such as laboratory tables and chairs proper to elementary school settings. The science laboratory model was figured out in terms of the following six procedures; (1) Current status as well as problems of science laboratory and its facilities was identified by means of the questionnaires conducted to 201 elementary schools, using stratified cluster sampling tehnique, under the consideration of the school size and the regional characteristics across the country. (2) Collected were the anthropometric data with respect to sitting height, popliteal height, buttock popliteal length, elbow rest height, and back width from the 747 students, 4th-6th grade, in Seoul area. (3) It was measured the work apace necessary for individual student to perform his experiment. (4) Using the data of the process (2), we determined the optimal sizes of laboratory tables and chairs fitted for Korean elementary school students. (5) The optimal area of science laboratory for 48-60 students is determined in terms of the data obtained in (3) for individual work space in addition to the appropriate table size figured out by (4). (6) A practical and ideal model for a science laboratory in elementary school was designed according to the above procedures. For the optimal model of science laboratory, the results of this study can be summarized as follows: The sizes of chair and table are categorized into three groups such as small, medium, and large depending on students' physical outfit. The small size base on the 12.5th percentile point of students' sitting height is used for students of the 0-25th percentile ranks. The medium size base on the 50th percentile point of students' sitting height is used for students of the 26-75th percentile ranks. The large size base on the 87.5th percentile point of students' sitting height is used for students of the 76-100th percentile ranks. (1) Sizes of chairs: The small size is 28cm in width and 33cm in height. The medium size is 31cm in width and 36cm in height. The large size is 35cm in width and 38cm in height. (2) Sizes of laboratory tables: The small size is 120cm in length, 86cm in width, and 60cm in height. The large size is 120cm in length, 86cm in width, and 60cm in height. The large size is 120cm in length, 86cm in width, and 65cm in height. (3) Size of science laboratory: The optimal science laboratory for 48-60 elementary school students, which can install the 12 laboratory tables, is 12m in length and 10m in width.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.97-106
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• 2020

The purpose of this study is to determine how to apply ISO/IEC/IEEE 29119 software test procedures and ISO/IEC 25023 software test evaluation items to software-accredited testing laboratories based on the ISO/IEC 17025 international standard requirements. As a method, the overall requirements for their application to a test laboratory were analyzed by mapping ISO/IEC/IEEE 29119 and ISO/IEC 25023 based on ISO/IEC 17025. According to the mapping analysis, a total of 29 documents were created, including quality manuals, procedures, and test guidelines for the operation of the test laboratory. As a result of the study, a test laboratory management system was developed using Confluence 6.15 software by applying the analyzed results of the overall requirements. The test procedures, documents, and records were stored in, and operated from, this system. Verification of suitability for operation of the test laboratory was conducted by KOLAS evaluators through a document review and an on-site audit at the LG Electronics SW Accredited Test Laboratory in Seoul. In conclusion, it was confirmed through the KOLAS evaluation that the test laboratory management system and the operation of the test laboratory are appropriate and, compared with previous cases, the test laboratory operates effectively, and the overall schedule of accreditation could be shortened.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.120-120
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• 2006

In our study grazing incidence X-ray scattering (GIXS) measurements with synchrotron radiation sources were conducted statically and in-situ for a series of nanoscale thin films prepared from nanoporous dielectrics, block copolymers, brush polymers, and molecular assemblies. All GIXS measurements were performed at the Pohang Accelerator Laboratory. The measured scattering data were analyzed in detail by using newly developed GIXS scattering theory. All GIXS results will be discussed in details with considering the materials chemistry and nanostructure formation process parameters.

• Journal of The Korean Association For Science Education
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• v.12 no.1
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• pp.93-101
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• 1992

The purposes of this study were; 1) to apply the network analysis to evaluation of students' laboratory reports, 2) to test the applicability of the systematic network analysis. For this purposes, 7th grade 88 students from 22 middle schools in Inchon city of Korea were selected and their laboratory reports were evaluated by a evaluation criterion network developed in this study. The evaluation criterion consisted of 4 categories and 10 subcategories. The results of this study suggested that the network analysis approach was suitable one for the evaluation of students' laboratory reports. This was supported by total score distribution, means/weight, Cronbach's ${\alpha}$ coefficient(0.70), and the subcategories' reliabilities. The resluts of the factor analysis indicated that the evaluation criterion network of this study had two factors.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.1144-1156
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• 2008

In this paper, heaving phenomenon is analyzed by laboratory tests. A laboratory test is consist of building soft clay foundation in plane-strain soil tank, construction of retaining wall, and excavation work. And range of shear strain, and destruction shape about soft clay foundation is compared, and analyzed with results of proposal formula. Using this study, safety factor is suggested for heaving phenomenon in the construction of wall on the soft clay. Actual theory is suggested by this suggested safety factor. There are various proposal formula for heaving phenomenon. For example, Terzaghi & Peck, Tschebotarioff, Bjerrum & Eide(Experience formula) and so on. Terzaghi & Peck's proposal formula is chosen, compared with laboratory test's result and analyzed in this study. A soft clay used in study is assumed homogeneous. A Depth of foundation is enough to observe shear strain by heaving phenomenon. Retaining wall is enough hard not to have vertical displacement.

• Proceedings of the Ginseng society Conference
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• 2005.05a
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• pp.7-7
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• 2005