• 제목/요약/키워드: Laboratory rearing

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Serological survey of diseases to poultry and swine in slaughtered ostriches (도축 타조에서 닭 및 돼지 질병에 대한 혈청학적 조사)

  • Kim Soon-Tae;Park In-Hwa;Kim Young-Hoan;Cho Kwang-Hyun;Oh Kyu-Shil;Son Jae-Kweon;Jyeong Jong-Sik
    • Korean Journal of Veterinary Service
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    • v.27 no.3
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    • pp.281-288
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    • 2004
  • As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

Project to increase productivity of livestock in Honam area against UR (호남지역의 양축농가에 있어서 UR에 대처한 가축의 생산성 향상에 관한 연구)

  • Lee, Joo-mook;Kwon, Oh-deog;Chae, Joon-seok;Kim, Myeong-chul;Kim, Heung-seob;Lee, Sung-jae;Lee, Hoo-sik;Roh, Soo-il;Kim, Kil-soo
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.195-212
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    • 1994
  • For the purpose to increase productivities of livestock the present investigations were undertaken in order to clarify the clinical and suclinical status of Korean native cattle, dairy cattle(holstein) and Korean native goat. Blood, feces and urine samples were collected from 247 Korean native cattle(222 adult and 25 calf), 224 dairy cattle(211 adult and 13 calf) and 142 Korean native goat rearing at Chonbuk area and analyzed for clinical, serum chemical, hematological and urinary findings. In addition, we were examined the infection rate of theileriosis, internal parasite and ring worm. The mean value for each component was calculated by statistical analysis using Excel computer program. From these investigations the following results were obtained. The mean values for RBC, PCV and etc in 433 adult cow(Korean native cattle and dairy cattle) were similar with other reports. But the mean values for MCHC of all species were lower than normal. Adult Korean native cattle and adult dairy cattle which showed hematologically normal levels were only 9.01% and 9.48%, respectively. Abnormally high values for PCV, RBC and Hb were recorded in 7.66% of adult Korean native cattle, 20% of korean native calf, 15.38% of dairy calf and 13.36% of Korean native goat. Adult Korean native cattle and adult dairy cattle which showed hematologically anemia were 4.95% and 19.43% respectively, but Korean native calf and dairy calf showed normal. Adult Korean native cattle, adult dairy cattle, Korean native calf and dairy calf which showed normal serum protein level 84.0%, 90.8%, 50% and 44.4%, respectively. In present investigations, 50% of Korean native calf and 55.6% of dairy calf were decreased serum protein values under range. These abnormally decreased serum protein values mean the shortage of antibody, and these have a possibility to occurs to pneumonia and diarrhea. From these results, the economical loss caused by pneumonia was calculated as 124,038,833 won in the KNC and 742,703,430 won in the dairy calf rearing in Chonbuk area. Calculated economical loss caused by enteritis was 56,658,690 won in Korean native cattle, 476,775,799 won in dairy calf, and the total loss amount of 533,434,488 won in Chonbuk area. Abnormally high values$(21.7{\pm}4.0mg/dl)$ for serum calcium were recorded 49.6% in dairy cattle. The mean values for serum total cholesterol were $170.8{\pm}99.8mg/dl$ in Korean native cattle, $196.0{\pm}40.6mg/dl$ in Korean native calf, $202.9{\pm}86.0mg/dl$ in adult dairy cattle and $289.4{\pm}97.5mg/dl$ in dairy calf. The infection rate of internal parsite were as follows; adult Korean native cattle: 21.2%, Korean native calf: 8.0%, adult dairy cattle: 67.8% and Korean native goat: 81.5%. The estimated economical loss caused by internal parasites infection were 1,120,855,837 won in Korean native calf, 4,994,959,405 won in adult Korean native cattle, 3,334,751,066 won in adult holstein, and the total loss amount to 9,450,566,308 won. The infection rate of theileriosis were 1.4% in Korean native cattle and 6.6% in dairy cattle. The presumed economical loss by T. sergenti infection were 154,408,482 won in Korean native cattle and 171,577,237 won in dairy cattle rearing at Chonbuk area. The infection rate of ringworm were 0.5% in Korean native cattle, 0.9% adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12,061,532 won in Korean native cattle, 16,895,403 won in dairy cattle, and the total estimated loss amount to 28,955,935 won a year in Chonbuk area.

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Increasing Production in Korean Shrimp Farms with White-Spot Syndrome Virus PCR-Negative Brood Stock

  • Seok, Seung-Hyeok;Baek, Min-Won;Lee, Hui-Young;Kim, Dong-Jae;Chun, Myung-Sun;Kim, Jong-Sheek;Chang, Se-Ok;Park, Jae-Hak
    • Journal of Microbiology and Biotechnology
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    • v.17 no.3
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    • pp.511-515
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    • 2007
  • White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSSV-PCR-negative fly epidemiologically decreases white-spot disease outbreaks. Here, we report a retrospective analysis of data from shrimp farms in the western coast of Korea where WSSV-PCR-negative brood stocks were used to stock rearing ponds. A total of 366 shrimp from Heuksan Island were sampled for WSSV with PCR. Of the tested shrimp, 7.2% (28 brood stocks) were identified as WSSV positive; only WSSV-PCR-negative shrimp were used for brood stocks. Total unit production (final shrimp production/ the area of the ponds) was higher, at 1.96, in ponds where WSSV-PCR-negative shrimp were used, as compared with 1.02 in other ponds in Korea in 2004. This retrospective analysis of WSSV in Korea may be useful to the shrimp aquaculture industry, suggesting a testable hypothesis that may contribute to the eventual control of WSSV outbreaks.

Five Genes Regulated by Oryctes rhinoceros nudivirus Infection in the Intestinal Tube of Allomyrina dichotoma (장수풍뎅이(Allomyrina dichotoma)에 Oryctes rhinoceros nudivirus 감염에 의해서 유전자 발현이 조절되는 5개의 유전자)

  • Yoo, Bo-Kyung;Kwon, Kisang;Ko, Young Hwa;Lee, Eun Ryeong;Choi, Ji-Young;Kwon, O-Yu
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1336-1340
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    • 2016
  • Korean rhinoceros beetles (Allomyrina dichotoma), which can be found in broad-leaved forests in mountainous habitats and lives for around one year in wild. This beetle is currently popular as a pet and traditionally regarded as a medicine for liver-related diseases in Korea. It is reported that the economic losses in the mass-rearing facilities by virus infection have been increased since the 2010s in Korea. The causing virus for the A. dichotoma was firstly reported as an Oryctes rhinoceros nudivirus (OrNV) in 2015. We, here, observes that serious morphological changes in the intestinal tube from the OrNV-infected beetles, and report five genes, which are regulated by OrNV infection in the intestine; Krueppel-like factor 15 (Klf15), Endoplasmic reticulum aminopeptidase 2 (ERAP2), U5 small nuclear ribonucleoprotein 200 kDa helicase (Snrnp200), Muscleblind-like protein 2a (mbnl2a), and MIMI_L93. The results may provide a clue to the early diagnosis and disease treatment during the mass-rearing facilities of the A. dichotoma.

Studies on Boring Characteristics and Mortality on the Valves of the Seed and Adult Meretrix petechialis by Glossaulax didyma didyma (Gastropoda: Naticidae) in the Shellfish Aquafarm and the Indoor Aquarium (패류양식장과 실내 사육수조에서 큰구슬우렁이 Glossaulax didyma didyma (Gastropoda: Naticidae)에 의한 말백합 종패와 성패의 패각 위 천공특징 및 폐사에 관한 연구)

  • Park, Young-Je;Kim, Sung-Han
    • The Korean Journal of Malacology
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    • v.27 no.2
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    • pp.131-141
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    • 2011
  • Boring caracteristics, boring rate, and predation, rates on the valves of the seed and adult clams of Meretrix petechialis by Glossaulax didyma didyma in the shellfish aquafarm and the indoor rearing aquarium were investigated by various morphometric data. The investigations were carried out from June to September, 2007. The morphology of drilled holes on the valves of M. petechialis showed the crater type as seen in the spats of Ruditapes philippinarum and M. lusoria. The sizes of the outer diameters of holes were greater than the inner diameters of the holes on the valves of M. petechialis in the shellfish aquafarm and the indoor aquarium at the laboratory. On the whole, the sizes of the holes on the valves of the seeds of M. petechialis was smaller than those bored holes on the adult valves. The location of the bored holes on the valves of the seeds varied widely with the the sizes of the individuals of M. petichialis, while most of holes on the valves of adult clams were located near the umbo position of the valves of the clams in the shellfish aquafarm and the indoor aquarium at the laboratory. On the whole, the outer and inner diameters of the bored holes increased with increase of shell lengths of the clams in the shellfish aquafarm and the indoor rearing aquarium at the laboratory. The authors could confirm experimentally the boring snail, G. didyma didyma, drilled the seeds and adult clams of M. petechialis. In the experiments for 15 days (three times repeated) in the indoor rearing aquarium at the laboratory, the mortality of dead shells to total shells of M. petechialis by boring snail Glossaulax didyma didyma was totally average 41.5%, and percent of drilled shells to total dead shells of clams by the boring snail was average 47.1%. Of total shells, percent of drilled shells to total shells of clams by the boring snail was totally average 19.3%. Predation of G. didyma didyma were greater at night than the day time. Average 0.5 seed and adult individuals of M. petewchialis were consumed per G. didyma didyma, per day. Predation varied with shell lengths of M. petechialis and shell widths of G. didyma didyma.

Historical Details about the Meat Consumption and Taeniases in Joseon Period of Korea

  • Shin, Dong Hoon;Chai, Jong-Yil;Hong, Jong Ha;Seo, Min
    • Parasites, Hosts and Diseases
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    • v.55 no.4
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    • pp.457-460
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    • 2017
  • Previous paleoparasitological studies of Joseon specimens established that the prevalence of Taenia infection was not much different from that of the early 20th century Korean population. As many of taeniases originally diagnosed as Taenia saginata in South Korea were revealed to be actually Taenia asiatica, which share a common intermediate host with T. solium (the pig), Joseon people must have ingested raw pork frequently. However, the current examination of extant Joseon documents revealed that the population ate significant amounts of beef even if the beef ban was enforced; and pork was not consumed as much as we thought. Considering the meat consumption pattern at that time, Joseon people should have been infected by T. saginata more frequently than T. asiatica. This may suggest a low prevalence of T. saginata metacestodes in cattle compared to that of T. asiatica metacestodes in pigs, possibly due to the traditional way of rearing pigs (using human feces). This letter gives us a chance to reconsider the existing preconception about parasitic infections in Korean history though we are still hard to accurately estimate the historical patterns of taeniases at this stage.

Effect of Spirulina platensis and Probiotics as Feed Additives on Growth of Shrimp Fenneropenaeus chinensis

  • Kim Choong-Jae;Yoon Sook-Kyung;Kim Hong-Ik;Park Yong-Ha;Oh Hee-Mock
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1248-1254
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    • 2006
  • The effect of Spirulina platens is and probiotics as feed additives on the growth of the shrimp Fenneropenaeus chinensis was investigated in comparison with a control. The shrimp were cultured in rearing tanks in a seawater pond for 35 days from September 1, 2004. As regards the water quality, the probiotic treatment (T2, commercial diet and 3% probiotics) produced a lower TDN (total dissolved nitrogen) and TDP (total dissolved phosphorus), making it effective in water quality improvement. Nonetheless, the phytoplankton flora succeeded from diatoms to cyanobacteria, regardless of the feed additives. Treatment T3, including 3% S. platensis, produced the highest mean body weight, which was 39% higher than that for all the other treatments (P<0.05). Accordingly, it was found that the use of Spirulina and probiotics as feed additives increased the shrimp body weight and improved the water quality, respectively.

Complete Larval Development of Chiromantes haematocheir (Crustacea: Decapoda: Brachyura: Grapsidae), with a Key to Seven Sesarminid Zoeas in Korea

  • Oh, Seong-Mi;Chung, Dah-Eun;Ko, Hyun-Sook
    • Animal Systematics, Evolution and Diversity
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    • v.23 no.1
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    • pp.9-20
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    • 2007
  • The complete larval development of Chiromantes haematocheir was obtained by laboratory rearing. Five zoeal and one megalopal stages are described and illustrated in detail. Morphological characteristics of the larvae slightly differ from those of Lee (1988), especially in the setal presence of mouthpart appendages. Seven sesarminid zoeas (Parasesarma acis, Parasesarma pictum, Perisesarma bidens, Sesarmops intermedius, Chiromantes dehaani, Chiromantes haematocheir, and Nanosesarma gordoni) can be distinguished from each other in characters of the carapace spines, the antennal exopod and the fork of telson. A provisional key is first provided to aid the identification of seven sesarminid zoeas in Korea.

Development of Resistance to Bombyx mori Densonucleosis Virus into a Susceptible Silkworm Breed

  • Singh, G.P.;Xu, Mengkui;Chen, Yuyin;Datta, R.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.6 no.2
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    • pp.145-149
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    • 2003
  • Seeing inadequate disinfection and unhygenic condition in rearing area, use of disease resistant silkworm variety is the best option. In order to this, an attempt has been made to develop the resistance to Bombyx mori densonucleosis virus (BmDNV-2) into a susceptible silkworm breed Zhenon1 by cross breeding with a resistant silkworm breed SU12 and exposing the subsequent generations to BmDNV-2 followed by the selection of individuals from the surviving batches. After seven generation the evolved DNV-2 resistant strain showed the significantly higher resistance to BmDNV-2 than control Zhenon1. The economic characters of both of the breeds were almost on par.

Development of the Leaf-Footed Bug, Anoplocnemis dallasi (Hemiptera: Coreidae) (장수허리노린재(Anoplocnemis dallasi)의 발육)

  • Park, Sang Ock
    • The Korean Journal of Ecology
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    • v.18 no.4
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    • pp.463-470
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    • 1995
  • Anoplocnemis dallasi Kiritchencho is a sap-sucker which feeds on tips of amorpha fruticosa Linne in Korea. A. Dallasi had one generation a year and overwintered as the young adult stage. Most overwintered survivors emerged in early-mid May to late May. Known host plants were reviewed, and new host records were added. Females mainly laid eggs in linear single chain masses on leaves of Miscanthus sinensis var. purpurascens. Nymphs (except the non-feeding first instars) fed on young shoots. First instar nymphs clustered on leaves or shoots where they hatched, and, if disturbed, reformed aggregations soon. The second to the fifth instar nymphs migrate to the upper part of the shoot and congreagate on a partly expanded leaf. New adults firstly appeared in late August, remained on A.fruticosa, host plant, and fed on until mid October. The duration and survivorship curve, in laboratory rearing, of the egg and each nymphal stadium was determined.

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