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Effects of 835-MHz Radiation on the Intracellular Calcium, Reactive Oxygen Species, and F-actin Polymerization in Rat-2 Fibroblasts

  • Hong Sae-Yong;Lee Zee-Won;Son Tae-Ho;Chang Sung-Keun;Choi Jong-Soon
    • Biomedical Science Letters
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    • v.12 no.1
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    • pp.9-16
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    • 2006
  • We investigated the effects of 835-MHz electromagnetic field (EMF), one of the most popular communication frequency band in Korean code-division multiple-access (CDMA) mobile phone system, on cellular signal transduction. For this, we examined the change of intracellular calcium $([Ca^{2+}]_i)$, reactive oxygen species (ROS) and F-actin polymerization after exposure to 835-MHz EMF followed by the treatment of agonists in Rat-2 fibroblast cells. Culture cells were pretreated with serum-tree medium and concomitantly exposed to 835-MHz at specific absorption rate (SAR) of 4.0 W/kg for 24 hr in a specialized designed apparatus based on Transverse Electro Magnetics (TEM) wave theory. Intracellular $Ca^{2+}$ responses to lysophosphatidic acid (LPA) and epidermal growth factor (EGF) in Rat-2 fibroblast after exposure to 835-MHz EMF were shown to be similar pattern as observed in normal cultured cells. However, the LPA-induced calcium spiking was slightly delayed to 7 sec and sustained thereafter to a little higher ground level under 835-MHz EMF radiation compared to unexposed cells. ROS production level by LPA in the exposed cells was not different from that in control. Furthermore, LPA induced the production of stress fibers with no significant difference in the exposed and unexposed cells. These results suggest that mobile phone radiation (835-MHz, SAR 4.0 W/kg) may not be directly related to signal transduction in Rat-2 fibroblasts except the slight effect of calcium spiking in LPA-induced cells but remain to be further elucidated for possible indirect intervention.

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Moleculay Cloning of the cDNA Encoding the 16 kDa Subunit of V-ATPase in Rat Brain (흰쥐 뇌에서 발현되는 16 kDa Vacuolar (H$^{+}$)-ATPase의 유전자 클로닝)

  • Shin, Song-Woo;Yoo, Min
    • Biomedical Science Letters
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    • v.6 no.3
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    • pp.165-170
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    • 2000
  • Vacuolar (H$^{+}$)-ATPase (V-ATPase) is an intracellular protein which consists of multiple subunits. It carries out acidification by pumping protons in the cell. This enzyme has also been found in the synaptic vesicles and may play an important role in the neurotransmission. We cloned cDNA fragments encoding the 16 kDa subunit of V-ATPase from the rat brain by RT-PCR and PCR using total RNA or recombinant phage DNA as templates. They contained the full coding sequences (468 bp) and one nucleotide at 3' region turned out to be different (A to C) when compared to the liver counterpart. However, this polymorphic difference did not cause any significant change in the primary structure of the protein because both GCA and GCC code for alanine. Our study would contribute to the understanding of the function of 16 M)a V-ATPase in the brain and of the mechanisms of neurotransmission.

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Assessment of Dose Distribution using the MIRD Phantom at Uterine Cervix and Surrounding Organs in High Doserate Brachytheraphy (자궁주위 방사선 근접치료시 MIRD 팬텀을 이용한 주변장기의 피폭환경평가)

  • Lee, Yun-Jong;Nho, Young-Chang;Lee, Jai-Ki
    • Korean Journal of Environmental Biology
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    • v.24 no.4
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    • pp.387-391
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    • 2006
  • Computational and experimental dosimetry of Henschke applicator with respect to high dose rate brachytherapy using the MIRD phantom and a remote control afterloader were performed. A comparison of computational dosimetry was made between the simulated Monte Carlo dosimetry and GAMMADOT brachytherapy Planning system's dosimetry. Dose measurements was performed using ion chamber in a water phantom. Dose rates are calculated using Monte Carlo code MCNP4B and the GAMMADOT. Thecomputational models include the detailed geometry of Ir-192 source, tandem tube, and shielded ovoids for accurate estimation. And transit dose delivered during source extension to and retraction from a given dwell position was estimated by Monte Carlo simulations. Point doses at ICRU bladder/rectal pointswhich have been recommened by ICRU 38 was assessed. Calculated and measured dose distribution data agreed within 4% each other. The shielding effect of ovoids leads to 19% and 20% dose reduction at bladder surface and rectal points.

Fast Search with Data-Oriented Multi-Index Hashing for Multimedia Data

  • Ma, Yanping;Zou, Hailin;Xie, Hongtao;Su, Qingtang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.7
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    • pp.2599-2613
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    • 2015
  • Multi-index hashing (MIH) is the state-of-the-art method for indexing binary codes, as it di-vides long codes into substrings and builds multiple hash tables. However, MIH is based on the dataset codes uniform distribution assumption, and will lose efficiency in dealing with non-uniformly distributed codes. Besides, there are lots of results sharing the same Hamming distance to a query, which makes the distance measure ambiguous. In this paper, we propose a data-oriented multi-index hashing method (DOMIH). We first compute the covariance ma-trix of bits and learn adaptive projection vector for each binary substring. Instead of using substrings as direct indices into hash tables, we project them with corresponding projection vectors to generate new indices. With adaptive projection, the indices in each hash table are near uniformly distributed. Then with covariance matrix, we propose a ranking method for the binary codes. By assigning different bit-level weights to different bits, the returned bina-ry codes are ranked at a finer-grained binary code level. Experiments conducted on reference large scale datasets show that compared to MIH the time performance of DOMIH can be improved by 36.9%-87.4%, and the search accuracy can be improved by 22.2%. To pinpoint the potential of DOMIH, we further use near-duplicate image retrieval as examples to show the applications and the good performance of our method.

A methodology for design of metallic dampers in retrofit of earthquake-damaged frame

  • Zhang, Chao;Zhou, Yun;Weng, Da G.;Lu, De H.;Wu, Cong X.
    • Structural Engineering and Mechanics
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    • v.56 no.4
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    • pp.569-588
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    • 2015
  • A comprehensive methodology is proposed for design of metallic dampers in seismic retrofit of earthquake-damaged frame structures. It is assumed that the metallic dampers remain elastic and only provide stiffness during frequent earthquake (i.e., earthquake with a 63% probability of exceedance in 50-year service period), while in precautionary earthquake (i.e., earthquake with a 10% probability of exceedance in 50-year service period), the metallic dampers yield before the main frame and dissipate most of the seismic energy to either prevent or minimize structural damages. Therefore by converting multi-story frame to an equivalent single-degree-of-freedom system, the added stiffness provided by metallic dampers is designed to control elastic story drifts within code-based demand under frequent earthquake, and the added damping with the combination of added stiffness influences is obtained to control structural stress within performance-based target under precautionary earthquake. With the equivalent added damping ratio, the expected damping forces provided by metallic dampers can be calculated to carry out the configuration and design of metallic dampers along with supporting braces. Based on a detailed example for retrofit of an earthquake-damaged reinforced concrete frame by using metallic dampers, the proposed design procedure is demonstrated to be simple and practical, which can not only meet current China's design codes but also be used in retrofit design of earthquake-damaged frame with metallic damper for reaching desirable performance objective.

Seismic behavior of composite walls with encased steel truss

  • Wu, Yun-tian;Kang, Dao-yang;Su, Yi-ting;Yang, Yeong-bin
    • Steel and Composite Structures
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    • v.22 no.2
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    • pp.449-472
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    • 2016
  • This paper studies the seismic behavior of reinforced concrete (RC) walls with encased cold-formed and thin-walled (CFTW) steel truss, which can be used as an alternative to the conventional RC walls or steel reinforced concrete (SRC) composite walls for high-rise buildings in high seismic regions. Seven one-fourth scaled RC wall specimens with encased CFTW steel truss were designed, manufactured and tested to failure under reversed cyclic lateral load and constant axial load. The test parameters were the axial load ratio, configuration and volumetric steel ratio of encased web brace. The behaviors of the test specimens, including damage formation, failure mode, hysteretic curves, stiffness degradation, ductility and energy dissipation, were examined. Test results indicate that the encased web braces can effectively improve the ductility and energy dissipation capacity of RC walls. The steel angles are more suitable to be used as the web brace than the latticed batten plates in enhancing the ductility and energy dissipation. Higher axial load ratio is beneficial to lateral load capacity, but can result in reduced ductility and energy dissipation capacity. A volumetric ratio about 0.25% of encased web brace is believed cost-effective in ensuring satisfactory seismic performance of RC walls. The axial load ratio should not exceed the maximum level, about 0.20 for the nominal value or about 0.50 for the design value. Numerical analyses were performed to predict the backbone curves of the specimens and calculation formula from the Chinese Code for Design of Composite Structures was used to predict the maximum lateral load capacity. The comparison shows good agreement between the test and predicted results.

A Study on Emission Characteristics of Mercury from Coal Combustion at a Lab-scale Furnace (실험용 연소로에서 석탄 연소 시 발생하는 수은 배출특성 연구)

  • Park, Kyu-Shik;Lee, Ju-Hyoung;Kim, Jeong-Hun;Lee, Sang-Hyeob;Seo, Yong-Chil
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.2
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    • pp.238-248
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    • 2008
  • This study investigated mercury emission at various combustion conditions and analyzed mercury species in flue gas from coal combustion at a laboratory scale furnace in coal. The results of this study can be used to predict and to assess mercury emission at coal boilers and power plants. The coal used in the plants generally contains about $0.02{\sim}0.28\;mg$ of mercury per kg. Bituminous and anthracite coal used for the experiment contained 0.049 and 0.297 mg/kg of mercury, respectively. Mercury emissions during coal combustion at temperatures range of $600^{\circ}C$ to $1,400^{\circ}C$ was measured and analysed using Ontario Hydro method; the speciation changes were also observed in mercury emissions. The results showed higher fraction of elemental mercury than that of oxidised mercury at most temperatures tested in this experiment. The fraction of elemental mercury was lower in combustion of anthracite coal than in bituminous combustion. As expected, equilibrium calculations and real power plants data showed good similarity. The distribution of particle size in flue gas had the higher peak in size above $2.5\;{\mu}m$. However the peak of mercury enrichment in dust was at $0.3\;{\mu}m$, which could be easily emitted into atmosphere without filtration in combustion system. When the CEA(Chemical equilibrium and Application) code was used for combustion equilibrium calculation, Cl was found to be the important component effecting mercury oxidation, especially at the lower temperatures under $900^{\circ}C$.

Addressing Mobile Agent Security through Agent Collaboration

  • Jean, Evens;Jiao, Yu;Hurson, Ali-R.
    • Journal of Information Processing Systems
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    • v.3 no.2
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    • pp.43-53
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    • 2007
  • The use of agent paradigm in today's applications is hampered by the security concerns of agents and hosts alike. The agents require the presence of a secure and trusted execution environment; while hosts aim at preventing the execution of potentially malicious code. In general, hosts support the migration of agents through the provision of an agent server and managing the activities of arriving agents on the host. Numerous studies have been conducted to address the security concerns present in the mobile agent paradigm with a strong focus on the theoretical aspect of the problem. Various proposals in Intrusion Detection Systems aim at securing hosts in traditional client-server execution environments. The use of such proposals to address the security of agent hosts is not desirable since migrating agents typically execute on hosts as a separate thread of the agent server process. Agent servers are open to the execution of virtually any migrating agent; thus the intent or tasks of such agents cannot be known a priori. It is also conceivable that migrating agents may wish to hide their intentions from agent servers. In light of these observations, this work attempts to bridge the gap from theory to practice by analyzing the security mechanisms available in Aglet. We lay the foundation for implementation of application specific protocols dotted with access control, secured communication and ability to detect tampering of agent data. As agents exists in a distributed environment, our proposal also introduces a novel security framework to address the security concerns of hosts through collaboration and pattern matching even in the presence of differing views of the system. The introduced framework has been implemented on the Aglet platform and evaluated in terms of accuracy, false positive, and false negative rates along with its performance strain on the system.

Development of Stress Based on Pore Pressure Model (응력 기반 간극수압 모델 개발)

  • Park, Du-Hee;Ahn, Jae-Kwang;Kim, Jin-Man
    • Journal of the Korean Geotechnical Society
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    • v.28 no.5
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    • pp.95-107
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    • 2012
  • Even though the importance of predicting build-up of pore pressure under cyclic loading is recognized, effective stress analysis is rarely performed due to difficulties in selecting the parameters for the pore pressure model. In this paper, a new stress based numerical model for predicting pore pressure under cyclic loading is developed. The main strength of the model is that it is easy-to-use, requiring only the CSR-N curve in selecting the parameters. Another advantage of the model is that it can be used for any loading pattern and therefore can be implemented in an effective stress time-domain dynamic analysis code. The accuracy of the model is validated through its comparisons with measurements in literature and laboratory test data collected in Korea. Further comparisons with another stress based pore pressure model highlighted the superiority of the proposed model.

A GateSensor for Effective and Efficient Entering/Taking Management of Vehicles for Construction Logistics (건설 자재 반출입 차량 관리 효율화를 위한 게이트센서)

  • Yoon, Su-Won;Song, Jae-Hong;Shin, Tae-Hong;Chin, Sang-Yoon
    • Korean Journal of Construction Engineering and Management
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    • v.12 no.1
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    • pp.85-96
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    • 2011
  • The access monitoring and control system of vehicles involved in construction logistics has been evolved from paper-based through bar code-based and to RFID-based management with pursuing automatic data collection and management. However, existing management methods have a limit that they still need manpower allocation for access control of vehicles. Therefore this research has proposed an framework that includes devices and an information system for effective and efficient data collection for access control of vehicles for construction logistics. And the proposed framework has been verified through a laboratory test using a prototype system developed in this research. It is expected that the installation methods of RFID antenna and tag for the framework could be applied to not only the access control of construction site but also various RFID applications at construction sites.