• Title/Summary/Keyword: LaMa

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Proliferation of Toxoplasma gondii Suppresses Host Cell Autophagy

  • Lee, Youn-Jin;Song, Hyun-Ouk;Lee, Young-Ha;Ryu, Jae-Sook;Ahn, Myoung-Hee
    • Parasites, Hosts and Diseases
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    • v.51 no.3
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    • pp.279-287
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    • 2013
  • Autophagy is a process of cytoplasmic degradation of endogenous proteins and organelles. Although its primary role is protective, it can also contribute to cell death. Recently, autophagy was found to play a role in the activation of host defense against intracellular pathogens. The aims of our study was to investigate whether host cell autophagy influences Toxoplasma gondii proliferation and whether autophagy inhibitors modulate cell survival. HeLa cells were infected with T. gondii with and without rapamycin treatment to induce autophagy. Lactate dehydrogenase assays showed that cell death was extensive at 36-48 hr after infection in cells treated with T. gondii with or without rapamycin. The autophagic markers, LC3 II and Beclin 1, were strongly expressed at 18-24 hr after exposure as shown by Western blotting and RT-PCR. However, the subsequent T. gondii proliferation suppressed autophagy at 36 hr post-infection. Pre-treatment with the autophagy inhibitor, 3-methyladenine (3-MA), down-regulated LC3 II and Beclin 1. The latter was also down-regulated by calpeptin, a calpain inhibitor. Monodansyl cadaverine (MDC) staining detected numerous autophagic vacuoles (AVs) at 18 hr post-infection. Ultrastructural observations showed T. gondii proliferation in parasitophorous vacuoles (PVs) coinciding with a decline in the numbers of AVs by 18 hr. FACS analysis failed to confirm the presence of cell apoptosis after exposure to T. gondii and rapamycin. We concluded that T. gondii proliferation may inhibit host cell autophagy and has an impact on cell survival.

Effect of heat-treatment parameter of YBCO film by TFA-MOD process (TFA-MOD법에 의한 YBCO 박막의 열처리변수 효과)

  • Jang, Seok-Hern;Lim, Jun-Hyung;Kim, Kyu-Tae;Lee, Jin-Sung;Yoon, Kyung-Min;Joo, Jin-Ho;Lee, Hee-Gyoun;Hong, Gye-Won
    • Progress in Superconductivity
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    • v.7 no.2
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    • pp.135-139
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    • 2006
  • We fabricated YBCO coated conductors (CCs) by TFA-MOD process and evaluated microstructure, texture formation, and critical temperature ($T_c$) and current ($I_c$). YBCO precursor solution was synthesized using metal-trifluoroacetates and dip coated on $LaAlO_3$(LAO) substrate. The phase formation and microstructure was characterized by X-ray diffraction and scanning electron microscopy (SEM) and the degree of texture was evaluated by pole-figure analysis. The CC was heat-treated in various calcining temperatures ($370^{\circ}C-460^{\circ}C$) and firing temperatures ($750^{\circ}C-800^{\circ}C$). As fired at $775^{\circ}C$ for 4h, the CC had the highest $T_c$ of 89.5 K and $I_c$ of 40 A/cm-width ($J_c=2.0\;MA/cm^2$). Microstructural observation indicated that the YBCO film was dense and homogeneous and had a strong cube texture without formation of second phase and its in-plane full-width at half-maxima; $5.2^{\circ}$ under optimum condition.

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New processing technique of TFA-MOD YBCO coated conductors using the '211' process (211 공정을 이용한 새로운 TFA-MOD YBCO 박막 선재 제조)

  • Lim, Jun-Hyung;Jang, Seok-Hern;Kim, Kyu-Tae;Lee, Jin-Sung;Yoon, Kyung-Min;Park, Eui-Cheol;Joo, Jin-Ho
    • Progress in Superconductivity
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    • v.7 no.2
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    • pp.140-144
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    • 2006
  • We fabricated the YBCO films on single crystal $LaAlO_3$ substrates via a metal organic deposition (MOD) process. In the process, $Y_2Ba_1Cu_1O_x$ and $Ba_3Cu_5O_8$ powders were dissolved in trifluoroacetic acid (TFA) followed by calcining and firing heat treatments. To evaluate the effects of the firing temperature on YBCO phase formation and critical properties, the films were fired at $750^{\circ}C,\;775^{\circ}C\;and\;800^{\circ}C$ after calcining at $430^{\cric}C$. Microstructure observation indicated that a crack-free surface formed and a strong biaxial texture was developed. The FWHM of out-of-plane texture was measured to be in the range of $4.3^{\cric}-7.0^{\circ}$ for all the films. When the YBCO film was fired at $775^{\cric}C$, it had the highest critical properties: 88.5 K of critical temperature and 16 A/cm-width of critical current ($1MA/cm^2$ as critical current density). On the other hand, those properties were degraded as firing at $750^{\circ}C\;and\;800^{\circ}C$. It is considered that the improved critical values are partly owing to dense and homogeneous microstructure, strong texture, and high oxygen content.

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Microstructural Observation of Multi-coated YBCO Films Prepared by TFA-MOD (TFA-MOD법으로 제조된 다층 YBCO 박막의 미세구조 관찰)

  • Jang, Seok-Hern;Lim, Jun-Hyung;Lee, Chang-Min;Hwang, Soo-Min;Choi, Jun-Hyuk;Shim, Jong-Hyun;Joo, Jin-Ho;Kim, Chan-Joong
    • Progress in Superconductivity
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    • v.9 no.2
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    • pp.167-172
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    • 2008
  • We fabricated $YBa_2Cu_3O_{7-x}$(YBCO) films on (00l) $LaAlO_3$ substrates prepared by metal organic deposition(MOD) method using trifluoroacetate(TFA) solution. The films with various thicknesses were prepared by repeating the dip-coating and calcining processes. The effects of film thickness on phase formation, microstructures, and critical properties were evaluated by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The microstructure and resultant critical current($I_C$) and critical current density($J_C$) varied remarkably with film thickness: The ($I_C$) value increased from 39 to 160 A/cm-width as the number of coatings increased from one to four, while the corresponding $J_C$ was measured to be in the range of $0.84-1.21\;MA/cm^2$. Both the $I_C$ and $J_C$ decreased when an additional coating was applied due to microstructural degradation, indicating that the optimum thickness is in the range of $1.1-1.8\;{\mu}m$. The possible cause for the decrease in the $I_C$ and $J_C$ value for film thicker than $1.8\;{\mu}m$ include non-uniform thickness, increased surface roughness, and the poor formability of the YBCO phase and texture arising from the insufficient heat treatment time with respect to the increased thickness.

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Fabrication of Thick SmBCO/IBAD-MgO coated conductor (후막 SmBCO/IBAD-MgO 초전도 박막선재의 제조)

  • Lee, J.H.;Kang, D.K.;Ha, H.S.;Ko, R.K.;Oh, S.S.;Kim, H.K.;Yang, J.S.;Jung, S.W.;Moon, S.H.;Youm, D.;Kim, C.J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.05a
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    • pp.9-9
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    • 2009
  • Coated conductor is required to have good critical current property for high efficiency of electric power applications. Until now, long coated conductor does not show high Jc over 3 MA/$cm^2$ in thick superconducting layer because of texture degradation by thick superconducting layer. In this study, in order to overcome this issue, thicker superconducting layer was deposited with optimized conditions to reduce the degradation of critical current density. SmBCO superconducting coated conductor was deposited with 1~3 um of thickness at $750\sim850^{\circ}C$ under 15~20 mTorr of oxygen partial pressure using batch type EDDC( evaporation using drum in dual chamber). The buffered substrate for superconducting layer deposition was used IBAD-MgO template with the architecture of $LaMnO_3/MgO/Y_2O_3/Al_2O_3$/Hastelloy. After fabrication of coated conductor, critical current was measured by 4-prove method under self-magnetic field and 77K. In addition, surface morphology and texture were analyzed by SEM and XRD, respectively. 3 um thick SmBCO coated conductor shows highest $I_C$ values of 638A/cm-w in 1 m long in the world.

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THE TURFAN MINARET INSCRIPTION: A SYMBOL OF CULTURAL CONFLUENCE ON THE SILK ROAD

  • VOSOOGHI, MOHAMMADBAGHER;KARIMIAN, HASSAN
    • Acta Via Serica
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    • v.2 no.1
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    • pp.31-47
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    • 2017
  • The corridors to the north and south of the $Takl{\bar{a}}m{\bar{a}}k{\bar{a}}n$ (塔克拉瑪干 Ta-ke-la-ma-gan) Desert are the most important regions for cultural confluence on the Silk Road, where caravans made it to the Chinese capital or the Korean Peninsula by the northern road, through the city of Turfan, or the southern path of Khutan. Being an important part of the Silk Road in the course of history, this region was heavily influenced by the cultures of various nations and ethnic communities whose merchants utilized the road to advance their business. The region's language, writing system and literary structure were also affected, so much so that in the course of its tumultuous history, many words, phrases and terms belonging to neighboring cultures found their way into the region, leaving their mark on its linguistic structure. Of the cultural exchanges that took place between the peoples of the region, conspicuous traces can be seen in the architecture, music, literature, texts, and inscriptions. Located in the Turfan region, the minaret of Su Gong (蘇公 Su Gong ) is host to an inscription which bears many signs of such exchanges. As so far no independent research has been conducted to identify the cultural, literary and structural features conveyed in this inscription, the present paper is an attempt to study the inscription in terms of the script, language and syntax in order to unravel the effects of cultures prevalent on the Silk Road on this particular inscription. This study mainly aims to investigate the linguistic structure of the inscription and the impact of the Persian language on Silk Road culture. In fact, we approach the inscription as a symbol of cultural exchange on the Silk Road and will focus on the tradition of Persian inscription-making which affected the Turfan inscription.

Detrital zircon U-Pb ages of the uppermost Jinju Formation in the Natural Monument No. 534 'Tracksite of Pterosaurs, Birds, and Dinosaurs in Hotandong, Jinju', Korea

  • Chae, Yong-Un;Lim, Jong Deock;Kim, Cheong-Bin;Kim, Kyung Soo;Ha, Sujin;Lim, Hyoun Soo
    • Journal of the Korean earth science society
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    • v.41 no.4
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    • pp.367-380
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    • 2020
  • LA-MC-ICP-MS zircon U-Pb dating was conducted to constrain the timing of fossil formation and the depositional age of the uppermost Jinju Formation located in Natural Monument No. 534 (Tracksite of Pterosaurs, Birds, and Dinosaurs in Hotandong, Jinju), and 87 Cretaceous, 1 Precambrian, and 2 Jurassic zircons were obtained from 90 valid analytical points. Most Cretaceous zircons were found to have a youngest graphical peak age of ca. 106.5 Ma, suggesting the depositional age of the uppermost Jinju Formation. Based on this study and previous works, the average sedimentation rate of the Jinju Formation was calculated to be approximately 0.17-0.31 mm/year in the Milyang Subbasin, and the Cretaceous zircons of the uppermost Jinju Formation seem to have originated mainly from the western or northwestern parts of the Gyeonggi Massif. Unlike the Nakdong and Hasandong formations of the Sindong Group, most zircons analyzed in the uppermost Jinju Formation were Cretaceous. This suggests that volcanic activity occurred in the area closer to the Gyeongsang Basin due to the roll-back of subducting paleo-Pacific Plates during the Jinju period.

Biomechanical Analysis of Golf Driver Swing Motion According to Gender

  • Bae, Kang Ho;Lee, Joong Sook;Han, Ki Hoon;Shin, Jin Hyung
    • Korean Journal of Applied Biomechanics
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    • v.28 no.1
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    • pp.1-8
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    • 2018
  • Objective: The purpose of this study is to investigate the differences in biomechanical variables of golf driving motion according to gender. Method: A total of 21 healthy golfers (11 men and 10 women) who have more than 5 years of professional experience and have been registered in the Korea Golf Association was recruited. A 250-Hz 8-camera motion capture system (MX-T20, Vicon, LA, USA) was used to capture the motion trajectories of a total of 42 reflective markers attached to the golfer's body and club. Moreover, two 1,000-Hz AMTI force plates (AMTI OR6-7-400, AMTI, MA, USA) were used to measure the ground reaction force. The mean and standard deviation for each parameter were then calculated for both groups of 21 subjects. SPSS Windows version 23.0 was used for statistical analysis. The independent t-test was used to determine the differences between groups. An alpha level of .05 was utilized in all tests. Results: There were differences in joint angles according to gender during golf driver swing. Men showed a statistically significantly higher peak joint angle and maximum range of angle in sagittal and frontal axis of the pelvis, hip, and knee. Moreover, women's swing of the pelvis and hips was found to have a pattern using the peak joint angle and range of angle in the vertical axis of the pelvis and hip. There were the differences in peak joint moment according to gender during golf driver swing. Men used higher joint moment in the downswing phase than women in the extensor, abductor, and external rotator muscles of the right hip; flexor and adductor muscles of left hip joint; and flexor and extensor muscles of the right knee. Conclusion: This result reveals that male golfers conducted driver swing using stronger force of the lower body and ground reaction force based on strength of hip and thigh than female golfers.

Geochemical Characteristics of the Cretaceous Jindong Granites in the Southwestern Part of the Gyeongsang Basin, Korea: Focussed on Adakitic Signatures (경상분지 서남부에 분포하는 백악기 진동화강암의 지화학적 특성:아다카이틱(adakitic)한 특성을 중심으로)

  • Wee, Soo-Meen;Choi, Seon-Gyu;Ryu, In-Chang;Shin, Hong-Ja
    • Economic and Environmental Geology
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    • v.39 no.5 s.180
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    • pp.555-566
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    • 2006
  • Major, trace and rare earth elements data of the Cretaceous Jindong granitic rocks were investigated in order to constrain the magma source characteristics and to establish the paleotectonic environment of the southwestern part of the Gyeongsang Basin. Geochemical signatures of the granitic rocks from the study area indicate that all of the rocks have characteristics of calc-alkaline series in the subalkaline field, and progressively shift from metaluminous to peraluminous with differentiation. In the variation diagrams, the overall geochemical features of the granites show systematic variations in major and trace elements. Chondrite normalized REE patterns show generally enriched LREE((La/Yb)c=4.2-12.8) and slight negative to flat Eu anomalies. Rb-Sr isotope data of the Jindong granites show that the whole rock age and Sr initial ratio are $114.6{\pm}9.1Ma$ and 0.70457, respectively. The Sr initial ratio of the Jindong granites is very similar to those of the Creataceous granites from Masan, Kimhae and Busan area($^{87}Sr/^{86}Sr=0.7049-0.707$). These results suggest that the magma have the mantle signature and intruded into the area during the early Cretaceous age. The Jindong granites have higher $Al_{2}O_{3},\;Na_{2}O$, Sr and lower $K_{2}O$, Y concentrations compared with typical calc-alkaline granitic rocks. These adakitic signatures are similar to those of adakitic pluton on Kyushu Island, southwest Japan arc. On the ANK vs. ACNK and tectonic discrimination diagrams, parental magma type of the granites corresponds to I-type, VAG granite. Interpretations of the geochemical characteristics of the granitic rocks favor their emplacement at continental margin during the subduction of Izanagi plate.

Study on the Technique of Weaving Fabrics in Korea( I ) -focused on hemp and ramie of the Three Kingdoms and the Korea Dynasty- (우리나라 직물제직기술에 대한 연구(I) -삼국시대와 고려시대의 대마와 저마직물을 중심으로-)

  • Min Gil Ja;Lee Soon Mee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.8 no.2
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    • pp.41-46
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    • 1984
  • Ancient Korean and China litteratures show us that it has been long since people from humbler classes to king most commonly used bast fabrics as materials of their cloth. According to the record of these ancient litteratures, highly developed fine bast fabrics and those woven in figures were used as tribute between ancient Korea and China. In this study we will make a brief research of the history of bast fabric culture from ancient times to the Korea Dynasty in which the bast fabric culture flourished and reached the summit of prosperity. After having compared the bast fabric culture of ancient Korea with that of ancient China in which the bast fabric culture developed very much and bast fabrics were used more commonly as materials of cloth than any other fabrics, we could come to conclusions as follows. 1. Names were given to bast fabrics according to the kinds of bast fibers, the degree of fineness and the containing of figures: Ma-po, Jeo, Jeo-po, Bag-jeo-po, Moon-jeo-po, Po and Se-po. The number of 'Seung' which indicated the degree of fineness was attached to the top of each name to show how fine they are. 2. While the bast fabric of 30 Seung is known to be the most fine one of the ancient China, in ancient Korea fine bast fabric of higher than 30 Seung was woven. This fact proves that the technique of weaving bast fabrics of the ancient Korea was more highly developed than that of ancient China. 3. In ancient China the highest Seung number of the ordinary clothes which were put on after putting off livery of grief was regulated to be 15. But in ancient Korea, Sil-la, it was regulated to be 28. Judging from this fact, we may say that the consumption level of the ancient Korean people in cloth was higher than that of the ancient China people. 4. The reason why in ancient Korea the technique of weaving bast fabrics was so highly developed is suppoed to be that the ancient Korean people preferred elegant, refine and simple taste in cloth. 5. The excellent bast fabric culture of the ancient Korea flowed into ancient Japan. It proves that Korea played an important part in the history of textile development of the world.

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