• Asian Pacific Journal of Cancer Prevention
• /
• 제15권23호
• /
• pp.10299-10306
• /
• 2015

The esophageal squamous cell carcinoma (ESCC) is thought to develop through a multi-stage process. Epigenetic gene silencing constitutes an alternative or complementary mechanism to mutational events in tumorigenesis. Posttranscriptional regulation of human leukocyte antigen class I (HLA-I) and antigen processing machinery (APM) proteins expression may be associated with novel epigenetic modifications in cancer development. In the present study, we determined the expression levels of HLA-I antigen and APM components by immunohistochemistry. Then by a bisulfite-sequencing PCR (BSP) approach, we identified target CpG islands methylated at the gene promoter region of APM family genes in a ESCC cell line (ECa109), and further quantitative analysis of CpG site specific methylation of these genes in cases of Kazakh primary ESCCs with corresponding non-cancerous esophageal tissues using the Sequenom MassARRAY platform. Here we showed that the development of ESCCs was accompanied by partial or total loss of protein expression of HLA-B, TAP2, LMP7, tapasin and ERp57. The results demonstrated that although no statistical significance was found of global target CpG fragment methylation level sof HLA-B, TAP2, tapasin and ERp57 genes between ESCC and corresponding non-cancerous esophageal tissues, there was significant differences in the methylation level of several single sites between the two groups. Of thesse only the global methylation level of LMP7 gene target fragments was statistically higher ($0.0517{\pm}0.0357$) in Kazakh esophageal cancer than in neighboring normal tissues ($0.0380{\pm}0.0214$, p<0.05). Our results suggest that multiple CpG sites, but not methylation of every site leads to down regulation or deletion of gene expression. Only some of them result in genetic transcription, and silencing of HLA-B, ERp57, and LMP7 expression through hypermethylation of the promoters or other mechanisms may contribute to mechanisms of tumor escape from immune surveillance in Kazakh esophageal carcinogenesis.

• Journal of Microbiology and Biotechnology
• /
• 제10권5호
• /
• pp.620-627
• /
• 2000

To determine the prevalence of genetic polymorphisms in Epstein-Barr virus (EBV) strains in the Korean population, the restriction site polymorphisms for BamHI and XhoI enzymes were analyzed with 16 EBV isolates from cancer patients and 7 EBV isolates from healthy carriers, using polymerase chain reaction techniques. None of the 23 isolates were found to carry an extra BamHI site in the BamHI F-fragment (f-variant). Of the 12 type-1 isolates from the cancer patients, 10 lost both the LMP1 XhoI site and the BamHI site between the BamHi W1* and I1* fragments (a W1*I1* fusion variant or type C). The latter W1*I1* fusion variant was due to a mutation of thymidine to adenine, as evidenced by a sequence analysis. The remaining two type-1 isolates showed either no variation at both sites or the loss of only the XhoI site. In contrast, two type-2 isolates and two intertypic recombinants with a type-1 allele at the EBNA2 locus and type-2 alleles at all or some of the EBNA3 loci retained both enzyme sites. In similar analyses of the 7 isolates from the healthy carriers, five of six type-1 isolates lost these two sites, however, one type-2 isolate did not. These results clearly indicate a strong association of both the LMP1 XhoI site loss and the W1*I1* fusion variant with the type-1 rather than the type-2 EBV strains circulating in the immunocompetent Korean carriers.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
• /
• pp.191-192
• /
• 2002

• 한국정보통신학회논문지
• /
• 제7권5호
• /
• pp.925-933
• /
• 2003

네트워크는 IP 계층과 광 계층이 수렴되는 광인터넷 네트워크로 진화할 것으로 예상되나, 현재의 기술수준으로 인해 과도적인 진화단계로서 GMPLS가 대두되었다. MPLS가 확장되고 일반화된 GMPLS는 패킷 교환 장치뿐만 아니라 시간, 파장 및 공간 영역에서 교환을 수행하는 장치까지 지원할 수 있도록 하고 있다. 이런 다양한 스위칭 타입에 공통적인 제어 평면을 구현하기 위해 GMPLS에서는 기존의 MPLS 신호 및 라우팅 프로토콜을 확장하게된다. 본 논문에서는 GMPLS 기술의 개요와 MPLS에서 링크의 상태 정보를 교환하기 위해 사용되었던 OSPF(Open shortest Path First) 프로토콜을 광 네트워크에서 다양한 링크 타입, 대역폭, 링크 보호 타입 등의 정보를 주고받기 위한 라우팅 확장 방안에 대하여 알아보고, 두 노드간의 수백, 수천개의 링크를 관리하는 복잡한 문제를 해결하기 위한 신호 프로토콜로 LMP라는 새로운 프로토콜의 정의를 기술한다. 그리고 MPLS에서 트래픽 엔지니어링을 위한 신호 프로토콜인 RSVP-TE를 GMPLS에 적용하기 위한 신호 프로토콜의 확장 방안과 수정된 RSVP 관련 메시지들을 검토분석 한다.

• 소음진동
• /
• 제7권3호
• /
• pp.427-437
• /
• 1997

Mean active intensity based active control for the cancellation of radiated noise out of the duct exit is studied. The active intensity control strategy is drerived based on the relation of the exterior sound field out of the duct termination and interior sound field of the duct. One of the characteristics of this control strategy is that the control performance can be maintained regardless of the sensor loction, compared with the conventional local pressure control methods at either interior downstream or exterior field positions. It is also suggested that the digital filtering for the active intensity control can be achieved by time-domain filtered-x LMP (Lest-Mean-Product) adaptive algorithm. Experiments for an open-ended duct are performed to compare the active intensity control performance with conventional pressure control one. Active control experiment of local sound pressure is conducted by widely used filtered-x LMS adaptive Algorithm and active intensity control implementaion uses the derived filter d-x LMP algorithm. It is shown that the exterior sound fileds was much better observable by sensing of the active intensity than by just sound pressure. It is also demonstrated that the global control performance of external field by acoustic intensity is superior to the conventional sound pressure control performance.

• 한국해양공학회지
• /
• 제15권2호
• /
• pp.46-52
• /
• 2001

In this paper, the real-time prediction of high temperature creep life was carried out for the friction welded joints of dissimilar heat resisting steels (SUH3-SUH35). various life prediction method such as LMP (Larson_miller Parameter) and ISM (initial strain method) were applied. The creep behaviors of those steels and the welds under static load were examined by ISM combined with LMP at 500, 600 and $700^{\circ}C$, and the relationship between these two methods was investigated. A real-time creep lie (tr, hr) prediction equation by initial strain (${\varepsilon}_0$, %) under any creep stress ($\sigma$, MPa) at any high temperature (T, K) was developed as follows: $t_r={\alpha}{\varepsilon}_0^{\beta}{\sigma}^{-1}$ where, ${\phi}=16: {\alpha}=10^{51.412-0.104T+5.375{\times}10^5T^2}$, $ {\beta}=-83.989+0.180T-9.957{\times}10^{-5}T^2,{\phi}=20:$ ${\alpha}=10^{69.910-0.146T+7.744{\times}10^{-5}T^2$, ${\beta}=-51.442+0.105T-5.595{\times}10^{-5}T^2$ for SUH3-SUH35 friction weld of =16mm and 20mm, respectively.

• 농업과학연구
• /
• 제48권4호
• /
• pp.655-668
• /
• 2021

The present study was conducted to investigate the effects of the supplementation of Achyranthes japonica extract (AJE) on overall performance levels with different nutrition concentrations in the diets of finishing pigs. Here, 140 finishing pigs with initial body weights of 50.18 ± 2.37 kg were used in a ten-week trial involving a 2 × 2 factorial design in two phases with the following factors: diet types with different nutrition concentrations (Positive control [PC] vs. Negative control [NC]) and 0 or 0.05% AJE supplementation to NC and PC diets. The PC diet was a basal diet, whereas the NC diet consisted of a 5% and 7% crude protein (CP) reduced basal diet during phase 1 (1 - 35 days) and phase 2 (36 - 70 days), respectively. A significant effect (p < 0.05) on the gain-to-feed ratio (G : F) was observed with interaction effects between the diets and AJE supplementation. However, during phase 1 and in all periods, the pigs fed the PC and NC diets with average daily feed intake (ADFI) tended to decrease compared to those fed diets with AJE. A significant effect (p = 0.0380) of E. coli was observed in pigs fed the PC and NC diets compared to AJE supplementation in phase 2 of the experiment. The backfat thickness (BFT) tended to decrease and the lean meat percentage (LMP) was significantly improved in phase 2 for pigs fed the PC and NC diets. In summary, BFT and LMP showed beneficial effects and fecal microbiota of E. coli counts were positively affected when pigs were fed diets with different nutrient concentrations.

• 전기학회논문지
• /
• 제57권7호
• /
• pp.1157-1166
• /
• 2008

This paper presents a new approach for the evaluation of location marginal prices (LMPs) considering demand-side bidding (DSB) in a competitive electricity market. The stabilization of the electric power supply and demand balance is one of the major important activities in electric power industry. In this paper, we present an analytical method for calculation of LMPs considering DSB, which has opportunity to compete with generating units, as England & Wales Pool's DSB scheme[1]. Also, we propose a new approach that LMP considering DSB is divided into three components. The proposed approach can be used for the evaluation of demand-side bidding into the electricity market and the assessment of the influence of DSB on total production costs and LMPs as well as three components.

• 한국식품영양과학회지
• /
• 제12권1호
• /
• pp.7-11
• /
• 1983

본 연구에서는 감귤류 폐과피의 효율적 이용을 위해, 온주밀감 과피내에 양질의 펙틴과 pectinesterase(PE)가 함께 존재하고 있는 사실에 근거하여 간단한 공정에 의한 저메톡실펙틴의 조제를 시도하였다. 한편 조제된 펙틴들의 물리화학적 특성과 gelling power를 조사하여 상품 펙틴과 비교하여 보았다. (1) 온주밀감 albedo를 갈아서 침출시키고, 이를 $30^{\circ}C$에서 $Na_2Co_3$로 pH를 조절하여 albedo내의 PE를 작용케 하였다. 가열에 의해 PE를 불활성화시키고 원심분리한 후 상층액에 알코올을 가해 저메톡실펙틴을 침전시키고, 알코올로 세척하여 조제하였다. (2) albedo내의 펙틴 수율은 건물 중량으로 12.71%~12.98%였다. (3) 각 펙틴 시료들의 메톡실 함량은 효소 작용을 받지않고 추출된 것이 13.71%로 고메톡실펙틴인 것으로 밝혀졌고, pH 7.5에서 10분 처리시 5.12%, pH 8.5에서 10분, 20분, 30분 처리됨에 따라 4.27%, 3.08%, 1.85%인 저메톡실펙틴이 조제되었다. 아세틸함량은 0.09~0.12%로서 겔 형성에 방해를 주는 범위는 아니였고, anhydrouronic acid 함량은 94.2~96.8%로 순도가 높았다. 효소 작용을 받는 정도가 많아짐에 따라 점도와 분자량은 감소하였다. (4) 저메톡실 펙틴들은 효소 작용을 받은 정도가 많음에 따라 consistency가 낮아졌으며 $18^{\circ}C$에서 측정한 값이 $4^{\circ}C$에서 측정한 값보다 낮았다. (5) 겔들의 %sag은 상품 펙틴겔보다 높았다. (6) Instron에 의한 texture 측정 결과는 고메톡실펙틴 겔의 경우 견고도와 탄성이 가장 높은 것으로 나타났다. 겔의 부착성은 pH 8.5에서 10분 처리한 것이, 응집성은 pH 7.5에서 10분 처리한 것이 가장 높았다. 겔들의 점착성과 저작성은 상품 저메톡실펙틴이 높았다.

• The Plant Pathology Journal
• /
• 제27권3호
• /
• pp.280-284
• /
• 2011

Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense is widespread in Indonesia. However, the distribution of tropical race 4 strains has not been well studied. Thirty nine isolates of F. oxysporum f. sp. cubense were collected from Java and 7 isolates were from Sumatera, Bangka, and Kalimantan. All isolates produced volatile odor when grown on steamed rice. These isolates were further tested for their vegetative compatibility with nitM testers of 20 reported vegetative compatibility groups representing strains that belong to race 1, 2, and 4. Three isolates formed heterokaryons with nitM testers belong to race 1, 11 isolates with race 4, and the rest did not form heterokaryons with all nitM testers used. F. oxysporum f. sp. cubense tropical race 4 specific primer pair was used to amplify a 1400 bp fragment of tropical race 4 DNA. Seven isolates (Bnt2, Mln1, Srg1, Bgl3, Bgl6, Lmp1, and Kjg1) produced the 1400 bp amplification product were therefore tropical race 4.