• 농업과학연구
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• 제46권4호
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• pp.827-841
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• 2019

The effect of genetically engineered rice Bt-9 on the diversity and abundance of plant-dwelling insects and spiders was tested under field conditions. Genetically engineered rice Bt-9, expressing mCry1Ac1 from Bacillus thuringiensis, confers resistance to rice leaf roller (Cnaphalocrocis medinalis) and provides tolerance to the herbicide glufosinate (PPT). The study compared Bt-9 and two non-GM reference varieties, Ilmi-byeo and Dongjin-byeo, at LMO isolated fields in Gunwi (Kyungpook National University) and Jeonju (National Institute Agricultural Sciences) in Southern Korea in 2016 - 2017. A total of 40,817 individuals from 62 families and 11 orders were collected from the two living modified organism (LMO) isolated fields. From the three types of rice fields, a total of 13,982, 14,105, and 12,730 individuals from the Bt-9, Ilmi-byeo and Dongjin-byeo were collected, respectively. Throughout the study, the analysis of variance indicated no significant differences (p < 0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects were similar. The data on insect species population densities were subjected to principal component analysis (PCA), which did not distinguish among the three varieties, Bt-9 and the non-GM, reference cultivars, during the cultivation years. However, the results of the PCA analysis were completely divided into four groups based on the yearly survey areas. Therefore, there was no evidence for a negative impact of Bt-9 on the above-ground insects and spiders.

• 농업과학연구
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• 제45권1호
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• pp.28-37
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• 2018

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

• 한국육종학회지
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• 제50권4호
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• pp.406-414
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• 2018

본 연구는 토양 세균인 B. thuringiensis에서 유래한 해충저항성 유전자(cry1Ac)를 일미벼에 도입시킴으로써 혹명나방(Cnaphalocrocis medinalis)에 대하여 살충성을 나타내는 해충저항성 Bt벼(Bt-T)의 환경위해성 평가에 대한 프로토콜 및 가이드라인을 개발하고자 수행하였다. 경북대학교 군위 LMO 격리포장에서 2년간 해충저항성 Bt벼(Bt-T)와 모품종인 동진벼 및 재배 품종인 일미벼를 재배하고, 혹명나방 등 표적곤충을 제외한 곤충류와 거미류가 포함한 다양성을 조사를 2016년과 2017년에 수행하였다. 조사기간 동안 채집된 개체들은 벼에 대한 기능별로 해충군, 천적군, 기타 곤충군으로 크게 구분하여 동정 계수하였으며, 2년간 총 11목 51과 9,552개체가 채집되었다. 해충저항성 Bt벼(Bt-T), 동진벼 및 일미벼에서 각각 3,042, 3,213, 3,297개체가 채집되었다. 조사된 개체군의 해충군, 천적군, 기타 곤충의 발생량은 해충군에서 노린목을 제외하고 해충저항성 Bt벼(Bt-T)과 모품종인 동진벼와 재배품종인 일미벼 품종 간의 각 분류군별 발생에서 통계적인 유의차가 없었으며, 시기별 곤충 발생 양상에서도 통계적인 유의적 차이를 보이지 않았다. 2년간의 곤충상 데이터를 주성분 분석법으로 분석한 결과, 2016년과 2017년 각각에서 동일하게 해충저항성 Bt벼(Bt-T)와 동진벼 및 일미벼 품종간의 곤충상 차이를 보이지 않았으나, 재배 시기에 따라 확연한 차이가 있음을 확인하였다. 유전자 변형에 의한 품종간의 차이 보다는 작물의 재배시기에 따라 곤충상 변화에 큰 영향을 끼치는 요인임을 새롭게 확인 할 수 있었다.