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In Vitro Selection and Characterizations of Gamma Radiation-Induced Salt Tolerant Lines in Rice (방사선을 이용한 내염성 계통의 기내선발 및 특징)

  • Lee, In-Sok;Kim, Dong-Sub;Hyun, Do-Yoon;Lim, Yong-Pyo;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • v.29 no.4
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    • pp.247-252
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    • 2002
  • The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

Quench Distribution in AU/YBCO Thin Film Meander Lines with a Au Meander Line Heater (금선 히터가 있는 금/YBCO 박막 선에서의 퀜치 분포)

  • Kim, H. R.;J. W. Shim;O. B. Hyun;J. M. Oh
    • Progress in Superconductivity
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    • v.5 no.2
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    • pp.118-123
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    • 2004
  • We investigated quench distribution in AU/YBCO thin film meander lines with a heater. Quench distribution during faults is important for superconducting fault current limter applications, because uniform quench allows application of higher voltages across the meander lines. AU/YBCO thin films grown on sapphire substrates were patterned into meander lines by photolithography. Gold films grown on the rear sides of the substrates were also patterned into meander lines, and used as heaters. Meander lines on the front and the rear sides were connected in parallel. The meander lines were subjected to simulated AC fault currents for quench measurements during faults. They were immersed in liquid nitrogen during the experiment for effective cooling. Resistance of the AU/YBCO meander lines initially increased more rapidly with the rear heater than without, and consequently the fault current was limited more. The resistance subsequently became similar, The resistance distribution was more uniform with the heater, especially during the initial quench. Quench was completed more uniformly and significantly earlier. This resulted in uniform distribution of dissipated power. These results could be explained with the concept of quench propagation, which was accelerated by heat transfer across the substrate from the rear heater.

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Metal Forming Simulation with Emphasis on Metal Flow Lines and its Applications (소성유동선도를 강조한 소성가공 시뮬레이션과 그 적용 사례)

  • Eom, J.G.;Jeong, S.W.;Joun, M.S.
    • Transactions of Materials Processing
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    • v.22 no.6
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    • pp.323-327
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    • 2013
  • In this paper, the flow lines as a function of product design as well as the forging process design are explored using typical application examples. The prediction of flow lines using metal forming simulation technology is introduced along with their characterization. Experimental studies have shown that the metal flow lines have a strong influence on the structural rigidity of the final product. In this study we present several typical applications. One example is the case of severely cut metal flow lines during machining, especially in the region where periodic contacting forces are applied. Another example is the case of abnormal distortion of flow lines which can cause too much elongation or hot shortness due to viscous heating in the region of distortion. A third example is the case of a macrosegregation region which needs to be controlled so it is not adjacent to the region where the force is applied in the use of the final component. An example of weight reduction for an automobile component with improved flow lines is also introduced. These typical applications can provide process engineers with the insight in designing automobile or mechanical components as well as in designing the manufacturing methods to produce various parts.

Mode Analysis of Cascaded Four-Conductor Lines Using Extended Mixed-Mode S-Parameters

  • Zhang, Nan;Nah, Wansoo
    • Journal of electromagnetic engineering and science
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    • v.16 no.1
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    • pp.57-65
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    • 2016
  • In this paper, based on the mode analysis of four-conductor lines, the extended mixed-mode chain-parameters and S-parameters of four-conductor lines are estimated using current division factors. The extended mixed-mode chain-parameters of cascaded four-conductor lines are then obtained with mode conversion. And, the extended mixed-mode S-parameters of cascaded four-conductor lines can be predicted from the transformation of the extended chain-parameters. Compared to the extended mixed-mode S-parameters of four-conductor lines, the cross-mode S-parameters are induced in the extended mixed-mode S-parameters of cascaded four-conductor lines, due to the imbalanced current division factors of cascaded two sections. The generated cross-mode S-parameters make the equivalent different- and common-mode conductors not independent from each other again. In addition, a new mode conversion, which applies the imbalanced current division factors, between the extended mixed-mode S-parameters and standard S-parameters is also proposed in this paper. Finally, the validity of the proposed extended mixed-mode S-parameters and mode conversion is confirmed by a comparison of the simulated and estimated results of shielded cable.

Thermal Cycling Screening Criteria to RCS Branch Lines in Domestic Nuclear Power Plant (국내 원전 RCS 분기배관에 대한 열피로 선정기준)

  • Park, Jeong Soon;Choi, Young Hwan;Lim, Kuk Hee;Kim, Sun Hye
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.6 no.2
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    • pp.54-60
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    • 2010
  • Piping failures due to thermal fatigue have been widely reported in normally stagnant non-isolable reactor coolant branch lines. Since the thermal fatigue due to thermal stratification was not considered in the piping fatigue design in old NPPs, it is important to evaluate the effect of thermal stratification on the integrity of branch lines. In this study, geometrical screening criteria for Up-horizontal branch lines in MRP-132 were applied to SI(Safety Injection) lines of KSNP 2-loop and WH 3-loop. Some computational fluid dynamic(CFD) analyses on the Reactor Coolant System(RCS) branch lines were also performed to develop the regulatory guidelines for screening criteria. As a result of applying MRP-132 screening criteria, KSNP 2-loop and WH 3-loop SI lines are determined to need further detailed evaluation. Results of CFD analyses show that both valve isolation and amount of leakage through valve can be used as technical bases for the screening criteria on the thermal fatigue analysis.

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Direct kinematic method for exactly constructing influence lines of forces of statically indeterminate structures

  • Yang, Dixiong;Chen, Guohai;Du, Zongliang
    • Structural Engineering and Mechanics
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    • v.54 no.4
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    • pp.793-807
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    • 2015
  • Constructing the influence lines of forces of statically indeterminate structures is a traditional issue in structural engineering and mechanics. However, the existing kinematic method for establishing these force influence lines is an indirect or mixed approach by combining the force method with the theorem of reciprocal displacements, which is yet inconsistent with the kinematic method for statically determinate structure. This paper proposes the direct kinematic method in conjunction with the load-displacement differential relation for exactly constructing influence lines of reaction and internal forces of indeterminate structures. Firstly, through applying the principle of virtual displacement, the formula for influence lines of reaction and internal forces of indeterminate structure via direct kinematic method is derived based on the released structure. Then, a computational approach with a clear concept and unified procedure as well as wide applicability based on the load-displacement differential relation of beam is suggested to achieve conveniently the closed-form expression of force influence lines, and exactly draw them. Finally, three representative examples for constructing force influence lines of statically indeterminate beams and frame illustrate the superiority of the proposed method.

Useful Image Back-projection Properties in Cameras under Planar and Vertical Motion (평면 및 수직 운동하는 카메라에서 유용한 영상 역투영 속성들)

  • Kim, Minhwan;Byun, Sungmin
    • Journal of Korea Multimedia Society
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    • v.25 no.7
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    • pp.912-921
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    • 2022
  • Autonomous vehicles equipped with cameras, such as robots, fork lifts, or cars, can be found frequently in industry sites or usual life. Those cameras show planar motion because the vehicles usually move on a plane. Sometimes the cameras in fork lifts moves vertically. The cameras under planar and vertical motion provides useful properties for horizontal or vertical lines that can be found easily and frequently in our daily life. In this paper, some useful back-projection properties are suggested, which can be applied to horizontal or vertical line images captured by a camera under planar and vertical motion. The line images are back-projected onto a virtual plane that is parallel to the planar motion plane and has the same orientation at the camera coordinate system regardless of camera motion. The back-projected lines on the virtual plane provide useful information for the world lines corresponding to the back-projected lines, such as line direction, angle between two horizontal lines, length ratio of two horizontal lines, and vertical line direction. Through experiments with simple plane polygons, we found that the back-projection properties were useful for estimating correctly the direction and the angle for horizontal and vertical lines.

Analysis of agricultural characteristics and qualities of CIMMYT resources in seeding time

  • Kang, Chon-Sik;Yoon, Young-Mi;Cheong, Young-Keun;Son, Jae-Han;Park, Jong-Chul;Kim, Yang-Kil;Park, Jong-Ho;Song, Tae-Hwa;Park, Tae-Il;Kim, Kyong-Ho;Kim, Bo-Kyeong
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.258-258
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    • 2017
  • We received 73 wheat resources from CIMMYT for analysis of agricultural characteristics and qualities of them. We sowed 73 lines and 4 Korean varieties in fall and spring to compare differences of wheat traits between fall and spring seeding. The heading and maturity date of fall seeding lines has coming faster average 18 days than spring seeding lines. Fall seeding lines have more number of spike per $m^2$ and grain number of spike. Spike and awn length of spring seeding lines were shorter than fall seeding lines and stem length was variable to varieties in both condition. Protein contents of five of 73 lines, Jokyoung and Jopoom varieties decreased but the others increased. The sedimentation values of 34 lines include Jokyoung and falling number (F/N) of 44 lines include two varieties, Keumkang and Jokyoung, decreased but the others increased, respectively. Particle size was presented that most spring seeding lines were bigger. However, when it sowed in fall, the lightness (L) of wheat flour was lighter. Consequently, we confirmed when it was sowed in spring, agricultural traits and qualities of wheat got weak.

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Development of a Simple Method to Determine the Mouse Strain from Which Cultured Cell Lines Originated

  • Yoshino, Kaori;Saijo, Kaoru;Noro, Chikako;Nakamura, Yukio
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.14.1-14.9
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    • 2010
  • Misidentification of cultured cell lines results in the generation of erroneous scientific data. Hence, it is very important to identify and eliminate cell lines with a different origin from that being claimed. Various methods, such as karyotyping and isozyme analysis, can be used to detect inter-species misidentification. However, these methods have proved of little value for identifying intra-species misidentification, and it will only be through the development and application of molecular biological approaches that this will become practical. Recently, the profiling of microsatellite variants has been validated as a means of detecting gene polymorphisms and has proved to be a simple and reliable method for identifying individual cell lines. Currently, the human cell lines provided by cell banks around the world are routinely authenticated by microsatellite polymorphism profiling. Unfortunately, this practice has not been widely adopted for mouse cells lines. Here we show that the profiling of microsatellite variants can be also applied to distinguish the commonly used mouse inbred strains and to determine the strain of origin of cultured cell lines. We found that approximately 4.2% of mouse cell lines have been misidentified; this is a similar rate of misidentification as detected in human cell lines. Although this approach cannot detect intra-strain misidentification, the profiling of microsatellite variants should be routinely carried out for all mouse cell lines to eliminate inter-strain misidentification.

The Extraction of Table Lines and Data in Document Image (문서영상에서 표 구성 직선과 데이터 추출)

  • Jang, Dae-Geun;Kim, Eui-Jeong
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.3
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    • pp.556-563
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    • 2006
  • We should extract lines and data which consist of the table in order to classify the table region and analyze its structure in document image. But it is difficult to extract lines and data exactly because the lines are cut and their lengths are changed, or characters or noises are merged to the table lines. These problems result from the error of image input device or image reduction. In this paper, we propose the better method of extracting lines and data for table region classification and structure analysis than the previous ones including commercial softwares. The prposed method extracts horizontal and vertical lines which consist of the table by the use of one dimensional median filter. This filter not only eliminates the noises which attach to the line and the lines which are orthogonal to the filtering direction, but also connects the cut line of which the gap is shorter than the length of the filter tap in the process of extracting lines to the filtering direction. Furthermore, texts attached to the line are separated in the process of extracting vertical lines. This is an example of ABSTRACT format.