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Prediction on the Quality of Total Mixed Ration for Dairy Cows by Near Infrared Reflectance Spectroscopy (근적외선 분광법에 의한 국내 축우용 TMR의 성분추정)

  • Ki, Kwang-Seok;Kim, Sang-Bum;Lee, Hyun-June;Yang, Seung-Hak;Lee, Jae-Sik;Jin, Ze-Lin;Kim, Hyeon-Shup;Jeo, Joon-Mo;Koo, Jae-Yeon;Cho, Jong-Ku
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.3
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    • pp.253-262
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    • 2009
  • The present study was conducted to develop a rapid and accurate method of evaluating chemical composition of total mixed ration (TMR) for dairy cows using near infrared reflectance spectroscopy (NIRS). A total of 253 TMR samples were collected from TMR manufacturers and dairy farms in Korea. Prior to NIR analysis, TMR samples were dried at $65^{\circ}C$ for 48 hour and then ground to 2 mm size. The samples were scanned at 2 nm interval over the wavelength range of 400-2500 nm on a FOSS-NIR Systems Model 6500. The values obtained by NIR analysis and conventional chemical methods were compared. Generally, the relationship between chemical analysis and NIR analysis was linear: $R^2$ and standard error of calibration (SEC) were 0.701 (SEC 0.407), 0.965 (SEC 0.315), 0.796 (SEC 0.406), 0.889 (SEC 0.987), 0.894 (SEC 0.311), 0.933 (SEC 0.885) and 0.889 (SEC 1.490) for moisture, crude protein, ether extract, crude fiber, crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF), respectively. In addition, the standard error of prediction (SEP) value was 0.371, 0.290, 0.321, 0.380, 0.960, 0.859 and 1.446 for moisture, crude protein, ether extract, crude fiber, crude ash, ADF and NDF, respectively. The results of the present study showed that the NIR analysis for unknown TMR samples would be relatively accurate. Use of the developed NIR calibration curve can obtain fast and reliable data on chemical composition of TMR. Collection and analysis of more TMR samples will increase accuracy and precision of NIR analysis to TMR.

Estimation of Availability and TDN of Various Silages by Cattle (소에 의한 다양한 사일리지의 이용율 및 TDN 평가)

  • Ji, Byung-Ju;Jin, Guang-Lin;Shinekhuu, Judder;Qin, Wei-Ze;Oh, Young-Kyoon;Sohn, Yong-Suk;Seo, Sung;Song, Man-Kang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.2
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    • pp.169-178
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    • 2010
  • The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 mg/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate ($C_2$) proportion, however, was greater (p<0.001~p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest $C_2$ proportion was observed from SGS. The butyrate proportion was lowest (p<0.007~p<0.027) in the cattle fed BS at 1~6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

Comparison of X-ray VCUG with RI VCUG for Diagnosing VUR in Children (소아의 방광 요관 역류 진단시 X-ray 배뇨성 방광 요도 조영술(X-ray VCUG)과 방사성 동위원소 배뇨성 방광 요도 조영술(RI VCUG)의 비교)

  • Hong Hyun-Soook;Choi Deuk-Lin;Kim Eun-Mi;Kim Sung-Jun
    • Childhood Kidney Diseases
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    • v.3 no.2
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    • pp.187-195
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    • 1999
  • Purpose : VUR is state where urine regurge from bladder to ureter and kidney. It is shown in about 1/3 of urinary tract infection patients and it is classified as grade I to V. We compared results from RI VCUG(Radiisotope voiding cystourethrography) and X-ray VCUG which used in diagnosing VUR in children, to evaluate which is better in diagnosing VUR in children. Methods : 41 Patients(19 males, 21 females), who visited Pediatric department, Soonchunhyang university Hospital from peroid of 1991. January to 1998. July for recurrent urinary tract infection or abnormalities in ultrasonogams, were enrolled in the study. The age ranged from 9 months to 17 years and mean age was 5 1/2 years. Both RI VCUG and X-ray VCUG were done and follow-up test of urine culture, renal ultrasonogram and RI VCUG were done every month, every 3 month and every 6 month, respectively to observe the disappearance of VUR and evaluated the prognosis. Results : 24 patients had taken RI VCUG and 17(70.1%) patients showed positive result. 22 patients had taken X-ray VCUG and 9(40.1%) patients showed findings of VUR. 17 patients had taken both tests and 14 patients showed positive result in RI VCUG and 6 of these patients also showed reflux in X-ray VCUG. 3 patients who showed negative in RI VCUG, showed negative also in X-ray VCUG. For prognosis, resolution and scar formation was shown in 8 patients each. Persistent VUR was shown in 6 patients and 2 of these patients VUR was corrected by operation, 1 patient showed decreased renal function, and 1 patient was not follwed up. 8 of 9 patients who showed findings of VUR on DMSA scan formed a scar and 8 patients who showed no findings of VUR didn't form a scar. Urine culture was positive in 17 of 19 patients with VUR. Positive rate in urine culture was higher than that of patients with no VUR who showed positivity in 15 of 21 patients for urine culture. E. coli was most common organism and the period free of UTI was 14 months in VUR patients and it was shorter compared to patients without VUR which was 26 months. Conclusion : In diagnosing VUR in children, the positive rate was higher in RI VCUG than X-ray VCUG. Therefore, in early diagnosis when VUR is suspicious but not shown in X-ray VCUG, RI VCUG should be done and it will help to make accurate diagnosis.

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The auditory evoked potential in premature small for gestational age infants (미숙아로 태어난 부당 경량아의 청각유발전위검사)

  • Moon, Il Hong;Ha, Kee Soo;Kim, Gui Sang;Choi, Byung Min;Eun, Baik-Lin;Yoo, Kee Hwan;Hong, Young Sook;Lee, Joo Won
    • Clinical and Experimental Pediatrics
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    • v.49 no.12
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    • pp.1308-1314
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    • 2006
  • Purpose : This study aimed to evaluate the usefulness of auditory evoked potential (AEP) in clarifying neuronal development in premature small for gestational age (SGA), and appropriate for gestational age (AGA) infants. Methods : A total of 183 premature infants who were born from August 2002 to July 2005, were examined with AEP. They were divided into three groups; AGA, symmetric-SGA and asymmetric-SGA group. Results : Statistically significant differences in the head circumference were observed in three groups. Among the risk factors, prevalence of hypoglycemia and hypoalbuminemia between AGA and asymmetric SGA infants were significantly different. V absolute peak latency (APL) in the right side of AGA infants was delayed were than that of asymmetric SGA infants. III-V interpeak latency (IPL) of asymmetric SGA infants was delayed more than that of symmetric SGA infants. Moreover, I-V IPL on both sides of symmetric SGA infants was shortened more than that of AGA infants. However, all the results of AEP were within the reference range, according to gestational age. Birth weight of, only asymmetric SGA, was related to the III APL on both sides and the III-V IPL on right side. Conclusion : This study shows that the values of APL and IPL of premature SGA infants are different than that of premature AGA infants. These data could be an indicator in evaluating the neurologic functions of small for gestational age infants.

Tibial Torsion in Children of the Jeju Area (제주지역 소아의 경골 염전)

  • Song, Dong Ho;Eun, Baik-Lin;Park, Sang Hee;Lee, Joon Young;Tockgo, Young Chang
    • Clinical and Experimental Pediatrics
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    • v.48 no.1
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    • pp.75-80
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    • 2005
  • Purpose : Internal tibial torsion is prevalent in East Asian countries such as Korea and Japan, where sitting on the floor is common behavior. Internal tibial torsion or excessive lateral tibial torsion may cause esthetical, functional, or psychological problems and also may induce degenerative arthritis in older age. The purpose of this study is to measure the tibial torsion in children of the Jeju area. Methods : Tibial torsion was measured in 1,042 lower extremities of 521 children from one to 12 years of age. The values of transmalleolar angles were analyzed for each age group divided by 6 months. Quadratic and linear regression models were used to fit patterns of changes in mean values of transmalleolar angles. The age at seven, which provides the highest coefficient of determination for quadratic regression analysis, was used as a cut-off point to fit different statistical models. Results : The mean transmalleolar angle was $0.10{\pm}5.79^{\circ}$ in all children,$ 0.90{\pm}5.49^{\circ}$ in males, and $-0.80{\pm}5.97^{\circ}$ in females. The value was $4.25{\pm}4.04$ in 1 year of age, gradually decreased to the lowest level of $-1.98^{\circ}$ in four years and seven months of age, increased again with age until it reached $0.67{\pm}1.10^{\circ}$ at seven years of age, and stayed at that level thereafter. Conclusion : Internal tibial torsion in infancy is known to correct spontaneously in the normal developing process. But in this study, the mean transmalleolar angle in children of Jeju area annually decreased after one year of age; to the lowest angle at four years and seven months of age; increased again gradually to the age of seven; and persisted in that level, about $10^{\circ}$ less than western children, not correcting further thereafter. These findings suggest tibial torsion might be caused by lifestyle, especially sitting on feet. To prevent abnormalities of joints and gaits, early diagnosis of tibial torsion in childhood and posture correction or early treatment when needed, seems to be necessary.

The effects of ibuprofen and indomethacin therapy for patent ductus arteriosus in preterm infants (미숙아 동맥관 개존 치료에서 indomethacin과 ibuprofen의 효과)

  • Choi, Wooksun;Shin, Jung-Yeon;Choi, Byung Min;Eun, Baik-Lin;Yoo, Kee Hwan;Hong, Young Sook;Lee, Joo Won
    • Clinical and Experimental Pediatrics
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    • v.49 no.12
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    • pp.1315-1323
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    • 2006
  • Purpose : This study was conducted to compare the efficacy and safety of ibuprofen and indomethacin in the treatment of patent ductus arteriosus (PDA) in preterm infants and to determine whether ibuprofen can be an alternative drug. Methods : A total of 32 preterm infants with symptomatic PDA were enrolled in the study. Twelve infants received intravenous ibuprofen 10 mg/kg, followed by 5 mg/kg after 24 and 48 hours. As a comparative group, twenty premature infants received three doses of indomethacin 0.1-0.2 mg/kg every 12 hours. Results : PDA was closed in 11 of 12 infants of the ibuprofen group (92 percent) and in 18 of 20 infants of the indomethacin group (90 percent). Serum sodium concentration decreased along with time significantly (P<0.0001) and to its lowest level at 48 hours after administration of the third dose (P=0.0011) in both groups, but showed no significant difference between two groups. Serum BUN and creatinine concentrations were not changed significantly before or after treatment in each group and showed no difference between thetwo groups. The amount of urine output did not change along with time significantly in both groups (P=0.0725), and showed no significant difference between two groups. Conclusion : Ibuprofen has similar effects to indomethacin in the rate of PDA closure and complication when compared. It has similar changes in serum sodium level and complications when compared to indomethacin for the treatment of PDA in preterm infants. Therefore, intravenous ibuprofen may be used as an alternative agent in the treatment of symptomatic PDA in preterm infants.

Functional recovery after transplantation of mouse bone marrow-derived mesenchymal stem cells for hypoxic-ischemic brain injury in immature rats (저산소 허혈 뇌 손상을 유발시킨 미성숙 흰쥐에서 마우스 골수 기원 중간엽 줄기 세포 이식 후 기능 회복)

  • Choi, Wooksun;Shin, Hye Kyung;Eun, So-Hee;Kang, Hoon Chul;Park, Sung Won;Yoo, Kee Hwan;Hong, Young Sook;Lee, Joo Won;Eun, Baik-Lin
    • Clinical and Experimental Pediatrics
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    • v.52 no.7
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    • pp.824-831
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    • 2009
  • Purpose : We aimed to investigate the efficacy of and functional recovery after intracerebral transplantation of different doses of mouse mesenchymal stem cells (mMSCs) in immature rat brain with hypoxic-ischemic encephalopathy (HIE). Methods : Postnatal 7-days-old Sprague-Dawley rats, which had undergone unilateral HI operation, were given stereotaxic intracerebral injections of either vehicle or mMSCs and then tested for locomotory activity in the 2nd, 4th, 6th, and 8th week of the stem cell injection. In the 8th week, Morris water maze test was performed to evaluate the learning and memory dysfunction for a week. Results : In the open field test, no differences were observed in the total distance/the total duration (F=0.412, P=0.745) among the 4 study groups. In the invisible-platform Morris water maze test, significant differences were observed in escape latency (F=380.319, P<0.01) among the 4 groups. The escape latency in the control group significantly differed from that in the high-dose mMSC and/or sham group on training days 2-5 (Scheffe's test, P<0.05) and became prominent with time progression (F=6.034, P<0.01). In spatial probe trial and visible-platform Morris water maze test, no significant improvement was observed in the rats that had undergone transplantation. Conclusion : Although the rats that received a high dose of mMSCs showed significant recovery in the learning-related behavioral test only, our data support that mMSCs may be used as a valuable source to improve outcome in HIE. Further study is necessary to identify the optimal dose that shows maximal efficacy for HIE treatment.

A Study on the form of korean Women's Hair Style-From the Viewpoint of Woman's Hair Style in Cho-Sun Dynasty- (한국 여성의 수발양식 관한 연구 -조선시대 여성 수발법을 중심으로-)

  • 정상숙;조효순
    • Journal of the Korean Society of Costume
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    • v.41
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    • pp.95-105
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    • 1998
  • SOO-BAL(Hair Style) is a method Which match hair style to face and clothes with using hair covering and protecting the head. Also SOO-BAL includes personal ornaments using to avoid one's hair be disheveled. In a standpoint of beauty and spirit, etiquette SOO-BAL is a very important thing as one being dressed up. Until now, since just a form of hair style have been studied, hair styling process is nothing to be known and studied. Time after time, our unique traditional SOO-BAL is forgotten with clothes and then this th-esis will be classified hair styling form follow-ing a form of hair style in royal palace of the C-hosun dynasty. According to the record of HAE DONG HISTORY, it shows the same of attire between Ko-rean and chinese style in ae of the chosun. The reason in that there were no any certain boundary border and the interaction of culture between two countries was happened spontaneously at ancient time like the GOCHO-SUN age. Until the period of the three states, the korean attire be changed had gone with chinese one s-imilarly. The chinese form gave to influence on the EONJIN MEURI·POON-GI-MYEONG MEURI·JJO-CJIN MEURI·MOOK-EUN GOONG-BAL MEURI·OL-LIN MEURI·SSANGSANG-TU ME-URI be drawn wall painting in the KOKUR-YU. And a gold chignon accesso-ry unearthed in a MOO-RYOUNG royal mausoleum is proof of the korean attrire be changed with chinese. In the shilla dynasty at three years after Cjin-Deuk(A.D. 649) reign. It was recorded that the dynasty let women wear the form of chinese attire. Also in the koryo dyn-asty, a rod-like hairpin (BIN-YEU) and DANG-GI employing EON-JIN MEURI was used. The SOO-BAL based on the Confucianism had lots of regulations which limited to use ornaments with classes of society in the CHOSUN dynasty. Until YOUNG CHO and CHUNG CHO period. EONJIN MEURI be decorated GACHAE was announced by dynasty as ind-ulging in luxury. Women of yangban used a rod-like hairpin and a chignon accessory made by jewerly. And 1-owly women weared a rod-like hairpin made of born and wood to perfom EONJIN MEURI with PUNCHAE. Most unmarried women decorated with DDA-AH-NEULIN MEURI, GUI-MIT MEURI, specially in palace with SAE-ANG MEURI. At palace, one put on a full dress with KEUN MEURI, and a simple dress with ER-YEO MEURI be decorated DDERL-JAM The CHOP-JI MEURI manifested social rank, class. Kids at CHO-SUN age had BA-DUK-PANMEURI and JONG-JONG MEURI. The ornament things are GACHE, DDERL JAM with EON-JIN M-EURI, and all kinds of rod-like hairpin and chignon accessory used in JJOK MEURI. IN DANGGE, JE-BI-BURI DANGGI used by ummarried women. DO-TOO-RAK DANGGI and AP DANGGI on a dress suit, and BE-SSI DANGGI used by 3∼4 years ungrown kids etc. were used. And at palace, kinds of CHUPJI used with JJOK MEURI showed social rank. In CHOSUN age, women want to keep shiny hair washed at TA-NO festival day, a treatment of bald hair used a forked remedy. In CHOSUN age, woman Soo-Bal hair style has DAE-SOO·DDEU-KOO-JI MEURI·CHO-P-GI MEURI·EON-JIN MEURI·SAE-ANG MEURI· and so on. We could find out Soo-Bal was developed very well by these variety hair styles. I attatched all of the hair style pictures step by step, and also explained detail my research foll owing these pictures.

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Development of a Nutrition Questionnaire and Guidelines for the Korea National Health Screening Program for Infants and Children (영유아 건강검진 영양 문진 및 지침 개발)

  • Moon, Jin-Soo;Kim, Jae-Young;Chang, Soo-Hee;Choi, Kwang-Hae;Yang, Hye-Ran;Seo, Jeong-Kee;Ko, Jae-Sung;Choi, Kyung-Dan;Seo, Jeong-Wan;Chung, Hee-Jung;Eun, Baik-Lin;Hwang, Seung-Sik;Seo, Soon-Ryu;Kim, Han-Suk
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.11 no.1
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    • pp.42-55
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    • 2008
  • Purpose: A new health policy, referred to as the National Health Screening Program for Infants and Children, was launched in November 2007 by the Ministry of Health and Welfare and National Health Insurance Corporation in Korea. We have developed a nutrition-counseling program that was incorporated into this project. Methods: We reviewed the nutritional guidelines published by The Korean Pediatric Society and internationally well-known screening programs such as Bright Future in the United States. We also reviewed the recent Korean national surveys on nutritional issues, including the Korea National Health and Nutrition Examination Survey (KNHANES) and the 2005 National Survey of Physical Body and Blood Pressure in Children and Adolescents. The development of questions, pamphlets, computer programs, and manuals for doctors was carried out after several meetings of researchers and governmental officers. Results: We summarized the key nutritional issues according to age, including breastfeeding in infants, healthier complementary feeding, and prevention of iron deficiency anemia, establishment of healthier diets, as well as dietary prevention of overweight children with an emphasis on physical exercise. We have constructed a new Korean nutrition questionnaire and an anticipatory guidance program based on the primary care schedule of visits at 4, 9, 18, 30, and 60 months of age. Five to eight questions were asked at each visit and age-matched pamphlets for parents and guidelines for doctors were provided. Conclusion: We developed a nutrition-counseling program based on recent scientific evidence for Korean infants and children. Further research on this national program for screening the nutritional problems in detail and setting the therapeutic approaches may help identify areas of success as well as those that need further attention.

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Urine Collection from Disposable Diapers in Infants and Young Children (유소아에서 일회용 기저귀를 이용한 소변 채취 방법)

  • Kim Mi Jeong;Kim Ji Hae;Yim Hyung Eun;Kang Hee;Eun Baik Lin;Yoo Kee Hwan;Hong Young Sook;Lee Joo Won;Kwon Junga
    • Childhood Kidney Diseases
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    • v.9 no.2
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    • pp.128-136
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    • 2005
  • Purpose : Urine collection using a sterile adhesive bag for urinalysis has been used commonly in infants and young children. However, this method has had some drawbacks. So, this study was performed to evaluate the usage of disposable diapers as a substitute for the routine urine collection method. Methods : 60 mL of self voided fresh urine was collected from 99 patients. Half of the urine was poured on the disposable diapers which did not contain absorbent gel beads. After 1 hr, we obtained the urine samples(extracted urine) by compressing the wet diapers using a 50 ml syringe. Routine, microscopic and biochemical analyses were performed on the other half of fresh urine and extracted urine. Then we compared each result by correlative analysis. Results : 198 samples from 99 patients were evaluated. The results of routine urinalysis except WBC and biochemical urinalysis showed a significant correlation between the two groups(P<0.05). The relative coefficients of urine SG, pH, glucose, protein, blood and leukocytes between the two groups were 0.964, 0.938, 0.977, 0.956, 0.931 and 0.738, respectively. Those of urinary sodium, potassium, chloride and creatinine were 0.997, 0.998, 0.995 and 0.998, respectively Microscopic examinations showed lower relative coefficients than the other results, 0.740 for RBC and 0.602 for WBC, but these were still significant(P<0.05). Conclusion : The results of the urine analysis with extracted urine from diapers correlates well with that of fresh urine. This new method is very helpful and can be used as an alternative of urine collection, especially for infants and young children. (J Korean Soc Pediatr Nephrol 2005;9:128-136)

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